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The map label for this gene is vanB [H]

Identifier: 66766651

GI number: 66766651

Start: 364258

End: 365238

Strand: Reverse

Name: vanB [H]

Synonym: XC_0307

Alternate gene names: 66766651

Gene position: 365238-364258 (Counterclockwise)

Preceding gene: 66766652

Following gene: 66766649

Centisome position: 7.09

GC content: 69.72

Gene sequence:

>981_bases
TTGACCGGCGAGGATGACGGCATGAGCCTGCATGAGGTGCGTGTGGCGGAAGTCGTCGACCAGGGACATCGCCAGCGGGC
GATCCGGTTGGAGCCGATCGGCGCGGAGTTGCCGGCCTTTGAAGCCGGCGCGCATGTGGACCTGCACCTGCCCGATGGTT
TGATCCGCCAGTACTCCATCGCCAGCGCGCCGCATGCGCGCGACCATTACCTGTTGTGCGTGAAGCTGGCCGACGCCTCG
CGCGGCGGCTCGCGGCATCTGTGCGAACAACTCGCGGCCGGCGACCGGCTGCAGATCTCCACGCCGCGCAACCTGTTCCC
GTTACACCCGGGCGAACGCCATGTGCTGCTGGCGGCCGGGATCGGCATCACTCCGCTGCTGTCGATGGTCGAGGCGCTGG
AAGCGCGCGGCGAGGCCTTCGTGCTGCACTACTACGCACGCCGGCAGGCGGATGTGGCCTTCGGGCAGCGCCTGCAGCAG
GGCTTCCAGCACGGCCAGGTGCAGGTGCACCTGAGCGATGGGGGCGAGAGCCCGCGTGTGCACATCCCCACCGAACTGCG
CAAGGCACACGCGCGCGACCAGCTGTATCTGTGCGGGCCGGCCGCGTTCATGGATCACTTCAGCGCGCTGGCCCTGGCGC
AGGGCTGGGCGCCGGCGCAGCTGCATCGCGAACACTTCGCCGCGGTCGCGCCGGCCGTGCCGCACGCCGCCGACGACGCC
TTCGAAGTTGAGCTGGCCGCCAGCGGGCGCGTGGTGCAGGTGGCGGCCGAGTGCAGTATCGCCAGTGCGCTGATGGCCGC
AGGCGTGGAGGTGCCGCTGTCGTGCGAGCAAGGCATGTGCGGGGCCTGCCTGACCGGCGTGCTCGATGGCGTGCCCGATC
ACCGCGACAGCGTGCTCAGCGACAGCGAACACGCGCAGAACACGCAGATCACCCTGTGCTGTTCGCGCAGCCGCACGCCA
CGGCTGGTGCTGGAACTTTGA

Upstream 100 bases:

>100_bases
CGCTGGACCCGCTGCTGGAAGTGCACATCCCCGCCGATCGCAGTTCGATCGCCTATCGCCGTGGCCTGCGCAGCCAGGGC
TATAGCGACTTTTTCCTGCG

Downstream 100 bases:

>100_bases
GCACCGCGGCAAGGCCGACACGTGATGCGACCGACGCGGCAACGATCAAGCGCGGGCGCGTTGGCGATGCTCGAACACGC
TGTGATCCATGTCCCCGCTA

Product: vanillate O-demethylase oxidoreductase

Products: 3,4-dihydroxybenzoate; NAD+; H2O; formaldehyde

Alternate protein names: Vanillate degradation ferredoxin-like protein [H]

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MTGEDDGMSLHEVRVAEVVDQGHRQRAIRLEPIGAELPAFEAGAHVDLHLPDGLIRQYSIASAPHARDHYLLCVKLADAS
RGGSRHLCEQLAAGDRLQISTPRNLFPLHPGERHVLLAAGIGITPLLSMVEALEARGEAFVLHYYARRQADVAFGQRLQQ
GFQHGQVQVHLSDGGESPRVHIPTELRKAHARDQLYLCGPAAFMDHFSALALAQGWAPAQLHREHFAAVAPAVPHAADDA
FEVELAASGRVVQVAAECSIASALMAAGVEVPLSCEQGMCGACLTGVLDGVPDHRDSVLSDSEHAQNTQITLCCSRSRTP
RLVLEL

Sequences:

>Translated_326_residues
MTGEDDGMSLHEVRVAEVVDQGHRQRAIRLEPIGAELPAFEAGAHVDLHLPDGLIRQYSIASAPHARDHYLLCVKLADAS
RGGSRHLCEQLAAGDRLQISTPRNLFPLHPGERHVLLAAGIGITPLLSMVEALEARGEAFVLHYYARRQADVAFGQRLQQ
GFQHGQVQVHLSDGGESPRVHIPTELRKAHARDQLYLCGPAAFMDHFSALALAQGWAPAQLHREHFAAVAPAVPHAADDA
FEVELAASGRVVQVAAECSIASALMAAGVEVPLSCEQGMCGACLTGVLDGVPDHRDSVLSDSEHAQNTQITLCCSRSRTP
RLVLEL
>Mature_325_residues
TGEDDGMSLHEVRVAEVVDQGHRQRAIRLEPIGAELPAFEAGAHVDLHLPDGLIRQYSIASAPHARDHYLLCVKLADASR
GGSRHLCEQLAAGDRLQISTPRNLFPLHPGERHVLLAAGIGITPLLSMVEALEARGEAFVLHYYARRQADVAFGQRLQQG
FQHGQVQVHLSDGGESPRVHIPTELRKAHARDQLYLCGPAAFMDHFSALALAQGWAPAQLHREHFAAVAPAVPHAADDAF
EVELAASGRVVQVAAECSIASALMAAGVEVPLSCEQGMCGACLTGVLDGVPDHRDSVLSDSEHAQNTQITLCCSRSRTPR
LVLEL

Specific function: Unknown

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=323, Percent_Identity=36.8421052631579, Blast_Score=181, Evalue=4e-47,
Organism=Escherichia coli, GI1787658, Length=316, Percent_Identity=27.5316455696203, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.14.13.82

Molecular weight: Translated: 35102; Mature: 34971

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGEDDGMSLHEVRVAEVVDQGHRQRAIRLEPIGAELPAFEAGAHVDLHLPDGLIRQYSI
CCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHH
ASAPHARDHYLLCVKLADASRGGSRHLCEQLAAGDRLQISTPRNLFPLHPGERHVLLAAG
CCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCCEEEEEEC
IGITPLLSMVEALEARGEAFVLHYYARRQADVAFGQRLQQGFQHGQVQVHLSDGGESPRV
CCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEE
HIPTELRKAHARDQLYLCGPAAFMDHFSALALAQGWAPAQLHREHFAAVAPAVPHAADDA
ECCHHHHHHHCCCCEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCC
FEVELAASGRVVQVAAECSIASALMAAGVEVPLSCEQGMCGACLTGVLDGVPDHRDSVLS
EEEEEECCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHCC
DSEHAQNTQITLCCSRSRTPRLVLEL
CCCCCCCCEEEEEECCCCCCEEEEEC
>Mature Secondary Structure 
TGEDDGMSLHEVRVAEVVDQGHRQRAIRLEPIGAELPAFEAGAHVDLHLPDGLIRQYSI
CCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHH
ASAPHARDHYLLCVKLADASRGGSRHLCEQLAAGDRLQISTPRNLFPLHPGERHVLLAAG
CCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCCEEEEEEC
IGITPLLSMVEALEARGEAFVLHYYARRQADVAFGQRLQQGFQHGQVQVHLSDGGESPRV
CCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEE
HIPTELRKAHARDQLYLCGPAAFMDHFSALALAQGWAPAQLHREHFAAVAPAVPHAADDA
ECCHHHHHHHCCCCEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCC
FEVELAASGRVVQVAAECSIASALMAAGVEVPLSCEQGMCGACLTGVLDGVPDHRDSVLS
EEEEEECCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHCC
DSEHAQNTQITLCCSRSRTPRLVLEL
CCCCCCCCEEEEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: vanillate; O2; NADH; H+

Specific reaction: vanillate + O2 + NADH + H+ = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9098058 [H]