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The map label for this gene is insH10 [H]

Identifier: 66766482

GI number: 66766482

Start: 162213

End: 163193

Strand: Reverse

Name: insH10 [H]

Synonym: XC_0133

Alternate gene names: 66766482

Gene position: 163193-162213 (Counterclockwise)

Preceding gene: 66766485

Following gene: 66766471

Centisome position: 3.17

GC content: 61.98

Gene sequence:

>981_bases
ATGCAACTGACGTTCGGTGACGCTGAGGGCCTGGGCAAGCGCAAGCAGACGCGCCGGGAGATCTTTCTGGCCGAGATGGA
GCAGGTCGTCCCGTGGCAGCAACTGCTCGGGCTGGTCGCGCCGCACTATCCGGTCTCGGGGCGGCCAGGTCGGCAGCCGT
ACGCACTGGCGACGATGTTGCGGATTCATCTGCTGCAGCAGTGGTATGCGTTGAGCGATCCGGCGATGGAAGAAGCACTG
CACGAGATCCCGACCTTGCGGCGCTTTGCCCAGCTCGGTGGCTTGGACAACGTTCCGGACGAGACCACGATTCTCAACTT
TCGCCGCCTGCTGGAGACCCATGGCCTTGCCGCGCGGATGCTGGAAGCGGTCAACGCGCATCTGGCGCGCAAGGGCCAGA
GCCTGCGGTCGGGCACGATCGTCGATGCGACGCTGATCGCGGCGCCCAGTTCGACCAAGAACGCCGACCATGCGCGCGAC
CCTGAGATGCATCAGACCAAGAAGGGCAATCAGTGGTATTTCGGGATGAAGGCGCACATCGGCGTGGATGAATTTTCCGG
GTTGGTGCACCACGTCCATTGCACAGCCGCCAACGTCGCCGATGTCACGGTGACGCACGCATTGCTGCATGGCAAAGAAG
ACAGCGTGTTTGGCGACAGCGGCTACACCGGTGCGGATAAGCGCGAAGAACTGCAGGACTGCGAGGCTGCATTTTTCATT
GCTGCCAAGCGTTCGGTGCTTCAAGCCATCGGCAACAAGCGGGAGCGTGCTCGGGAACAGCGCTGGGAACACTTCAAGGC
CAGCGTGCGTGCGAAGGTGGAGCATCCGTTCCGGGTGATCAAGCGCCAGTTCGGTTACACCAAGGTCCGCTATCGCGGCC
TGGCGAAGAACACCGCGCAGGTGCTGACGCTGTTTGCGCTGTCAAACCTGTGGATGAAGCGAAAGCAGTTATTGCCTGCT
ATGGGGAGCGTGCGCCTGTAA

Upstream 100 bases:

>100_bases
TGTGCATCCGCGACGGACCGCGGAATGCTGGATTTAAGGAACCTCTGAACAACGCACCACAGATACGCGACACTACCCGC
CTGATGTTGAGGGGTGATCC

Downstream 100 bases:

>100_bases
CGCAGGCAACACCCTGCAAACGCGCCGGAAACGGCAAAAAAATCGAGGATCTGAGCGCCGTCAGCCTGGCCGATGTAGTT
GGGCTCATCCTCCGACGCCG

Product: IS1479 transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MQLTFGDAEGLGKRKQTRREIFLAEMEQVVPWQQLLGLVAPHYPVSGRPGRQPYALATMLRIHLLQQWYALSDPAMEEAL
HEIPTLRRFAQLGGLDNVPDETTILNFRRLLETHGLAARMLEAVNAHLARKGQSLRSGTIVDATLIAAPSSTKNADHARD
PEMHQTKKGNQWYFGMKAHIGVDEFSGLVHHVHCTAANVADVTVTHALLHGKEDSVFGDSGYTGADKREELQDCEAAFFI
AAKRSVLQAIGNKRERAREQRWEHFKASVRAKVEHPFRVIKRQFGYTKVRYRGLAKNTAQVLTLFALSNLWMKRKQLLPA
MGSVRL

Sequences:

>Translated_326_residues
MQLTFGDAEGLGKRKQTRREIFLAEMEQVVPWQQLLGLVAPHYPVSGRPGRQPYALATMLRIHLLQQWYALSDPAMEEAL
HEIPTLRRFAQLGGLDNVPDETTILNFRRLLETHGLAARMLEAVNAHLARKGQSLRSGTIVDATLIAAPSSTKNADHARD
PEMHQTKKGNQWYFGMKAHIGVDEFSGLVHHVHCTAANVADVTVTHALLHGKEDSVFGDSGYTGADKREELQDCEAAFFI
AAKRSVLQAIGNKRERAREQRWEHFKASVRAKVEHPFRVIKRQFGYTKVRYRGLAKNTAQVLTLFALSNLWMKRKQLLPA
MGSVRL
>Mature_326_residues
MQLTFGDAEGLGKRKQTRREIFLAEMEQVVPWQQLLGLVAPHYPVSGRPGRQPYALATMLRIHLLQQWYALSDPAMEEAL
HEIPTLRRFAQLGGLDNVPDETTILNFRRLLETHGLAARMLEAVNAHLARKGQSLRSGTIVDATLIAAPSSTKNADHARD
PEMHQTKKGNQWYFGMKAHIGVDEFSGLVHHVHCTAANVADVTVTHALLHGKEDSVFGDSGYTGADKREELQDCEAAFFI
AAKRSVLQAIGNKRERAREQRWEHFKASVRAKVEHPFRVIKRQFGYTKVRYRGLAKNTAQVLTLFALSNLWMKRKQLLPA
MGSVRL

Specific function: Involved in the transposition of the insertion sequence IS5 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1787634, Length=311, Percent_Identity=54.0192926045016, Blast_Score=342, Evalue=2e-95,
Organism=Escherichia coli, GI1788303, Length=311, Percent_Identity=54.0192926045016, Blast_Score=340, Evalue=8e-95,
Organism=Escherichia coli, GI1789921, Length=311, Percent_Identity=53.6977491961415, Blast_Score=339, Evalue=1e-94,
Organism=Escherichia coli, GI1789612, Length=311, Percent_Identity=53.6977491961415, Blast_Score=339, Evalue=1e-94,
Organism=Escherichia coli, GI1789355, Length=311, Percent_Identity=53.6977491961415, Blast_Score=339, Evalue=1e-94,
Organism=Escherichia coli, GI1788518, Length=311, Percent_Identity=53.6977491961415, Blast_Score=339, Evalue=1e-94,
Organism=Escherichia coli, GI1788342, Length=311, Percent_Identity=53.6977491961415, Blast_Score=339, Evalue=1e-94,
Organism=Escherichia coli, GI1787592, Length=311, Percent_Identity=53.6977491961415, Blast_Score=339, Evalue=1e-94,
Organism=Escherichia coli, GI1786877, Length=311, Percent_Identity=53.6977491961415, Blast_Score=339, Evalue=1e-94,
Organism=Escherichia coli, GI1786764, Length=311, Percent_Identity=53.6977491961415, Blast_Score=339, Evalue=1e-94,
Organism=Escherichia coli, GI1786453, Length=311, Percent_Identity=53.6977491961415, Blast_Score=339, Evalue=1e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002559
- InterPro:   IPR008490 [H]

Pfam domain/function: PF05598 DUF772; PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 36732; Mature: 36732

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLTFGDAEGLGKRKQTRREIFLAEMEQVVPWQQLLGLVAPHYPVSGRPGRQPYALATML
CCCCCCCHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
RIHLLQQWYALSDPAMEEALHEIPTLRRFAQLGGLDNVPDETTILNFRRLLETHGLAARM
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHH
LEAVNAHLARKGQSLRSGTIVDATLIAAPSSTKNADHARDPEMHQTKKGNQWYFGMKAHI
HHHHHHHHHHCCCHHCCCCEEEHEEEECCCCCCCCCCCCCCCHHHCCCCCEEEEEEEEEC
GVDEFSGLVHHVHCTAANVADVTVTHALLHGKEDSVFGDSGYTGADKREELQDCEAAFFI
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AAKRSVLQAIGNKRERAREQRWEHFKASVRAKVEHPFRVIKRQFGYTKVRYRGLAKNTAQ
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VLTLFALSNLWMKRKQLLPAMGSVRL
HHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQLTFGDAEGLGKRKQTRREIFLAEMEQVVPWQQLLGLVAPHYPVSGRPGRQPYALATML
CCCCCCCHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
RIHLLQQWYALSDPAMEEALHEIPTLRRFAQLGGLDNVPDETTILNFRRLLETHGLAARM
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHH
LEAVNAHLARKGQSLRSGTIVDATLIAAPSSTKNADHARDPEMHQTKKGNQWYFGMKAHI
HHHHHHHHHHCCCHHCCCCEEEHEEEECCCCCCCCCCCCCCCHHHCCCCCEEEEEEEEEC
GVDEFSGLVHHVHCTAANVADVTVTHALLHGKEDSVFGDSGYTGADKREELQDCEAAFFI
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AAKRSVLQAIGNKRERAREQRWEHFKASVRAKVEHPFRVIKRQFGYTKVRYRGLAKNTAQ
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VLTLFALSNLWMKRKQLLPAMGSVRL
HHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9097039; 9097040; 9278503 [H]