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The map label for this gene is pdxJ

Identifier: 66766364

GI number: 66766364

Start: 14113

End: 14883

Strand: Reverse

Name: pdxJ

Synonym: XC_0012

Alternate gene names: 66766364

Gene position: 14883-14113 (Counterclockwise)

Preceding gene: 66766365

Following gene: NA

Centisome position: 0.29

GC content: 67.32

Gene sequence:

>771_bases
GTGAGCACTCACCTCAGCGTCAACGTCAACAAGATCGCCGTACTGCGCAATTCGCGCGGCGGTCAGGACCCGGATGTAGT
GCAGGCCGCGCGCAGCTGCATTGCAGCCGGCGCCCATGGCATCACCGTGCATCCGCGCCCGGACCAACGGCATATCCGCG
CTGACGATGTCTACGCGCTCAGCACACTGACGCGCATGCATGGTGTGGAATTCAACATCGAAGGCAATCCGTTCGCGCCG
CCGCGCGCTGGCTATCCGGGCTTGCTGGAACTCTGCCGGGCCACGCGGCCGCAACAGGTCACCCTGGTGCCGGATGGCGA
CGGCCAACTCACCTCCGACCATGGCGTGGATTTCGCCCGCGATGGCGCGCGGCTGGCGCCGTTGATTGCCGCCTTCAAGA
CTCTCGGCTGCCGCGTGAGCCTGTTCGTGGATGCCGGTAATCCGGAGATTGCGCAGGCGGCTGCGTTGGGTGCCGATCGC
ATCGAGCTGTACACCGGCCCGTACGCCGAGGCCCATCACCACGGCCAATCGCAACCGTCCCTGGCCCTGTTCGCCGATGC
CGCCAGGCGTGCCCACGCGGCCGGCCTGGGCATCAATGCCGGCCACGATCTGTCCCAGCACAACCTGGCCGATTTCCTGG
CCGGCGTTCCCGACGTCCTGGAGGTCTCGATCGGCCACGCCTTGGTCGGCGAGGCGCTGTATCAGGGCCTGGAACCGACC
GTGCGCGCCTACTTGGCGATTATTGCGGGCGGCGCGACCACAGCCGCCTAG

Upstream 100 bases:

>100_bases
GACCCAGCCGAACTGCGCCGGCTGCGCGAACTTGCCGAAGCCCGCAAGGCCGCTGCACTGACGGCCGCGCGCGACGGCGA
GCCGCAGGCCTGAGCAGCAC

Downstream 100 bases:

>100_bases
GCCCGGGTTTTAGCGAGCCGCGCGCAGTCCACGCAACATGCAGCTGCGCGTCCGTCATGCGCATCGGCCGCACAGTCGAG
CTTGCGATGCGTGCAATCGC

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MSTHLSVNVNKIAVLRNSRGGQDPDVVQAARSCIAAGAHGITVHPRPDQRHIRADDVYALSTLTRMHGVEFNIEGNPFAP
PRAGYPGLLELCRATRPQQVTLVPDGDGQLTSDHGVDFARDGARLAPLIAAFKTLGCRVSLFVDAGNPEIAQAAALGADR
IELYTGPYAEAHHHGQSQPSLALFADAARRAHAAGLGINAGHDLSQHNLADFLAGVPDVLEVSIGHALVGEALYQGLEPT
VRAYLAIIAGGATTAA

Sequences:

>Translated_256_residues
MSTHLSVNVNKIAVLRNSRGGQDPDVVQAARSCIAAGAHGITVHPRPDQRHIRADDVYALSTLTRMHGVEFNIEGNPFAP
PRAGYPGLLELCRATRPQQVTLVPDGDGQLTSDHGVDFARDGARLAPLIAAFKTLGCRVSLFVDAGNPEIAQAAALGADR
IELYTGPYAEAHHHGQSQPSLALFADAARRAHAAGLGINAGHDLSQHNLADFLAGVPDVLEVSIGHALVGEALYQGLEPT
VRAYLAIIAGGATTAA
>Mature_255_residues
STHLSVNVNKIAVLRNSRGGQDPDVVQAARSCIAAGAHGITVHPRPDQRHIRADDVYALSTLTRMHGVEFNIEGNPFAPP
RAGYPGLLELCRATRPQQVTLVPDGDGQLTSDHGVDFARDGARLAPLIAAFKTLGCRVSLFVDAGNPEIAQAAALGADRI
ELYTGPYAEAHHHGQSQPSLALFADAARRAHAAGLGINAGHDLSQHNLADFLAGVPDVLEVSIGHALVGEALYQGLEPTV
RAYLAIIAGGATTAA

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=239, Percent_Identity=40.1673640167364, Blast_Score=141, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_XANC8 (Q4V0R7)

Other databases:

- EMBL:   CP000050
- RefSeq:   YP_241126.1
- ProteinModelPortal:   Q4V0R7
- SMR:   Q4V0R7
- STRING:   Q4V0R7
- GeneID:   3382631
- GenomeReviews:   CP000050_GR
- KEGG:   xcb:XC_0012
- eggNOG:   COG0854
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- PhylomeDB:   Q4V0R7
- ProtClustDB:   PRK05265
- BioCyc:   XCAM314565:XC_0012-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 26800; Mature: 26669

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: ACT_SITE 44-44 ACT_SITE 74-74 ACT_SITE 202-202 BINDING 8-8 BINDING 19-19 BINDING 46-46 BINDING 51-51 BINDING 111-111 BINDING 203-203

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTHLSVNVNKIAVLRNSRGGQDPDVVQAARSCIAAGAHGITVHPRPDQRHIRADDVYAL
CCCEEEEECEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHH
STLTRMHGVEFNIEGNPFAPPRAGYPGLLELCRATRPQQVTLVPDGDGQLTSDHGVDFAR
HHHHHHHCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHC
DGARLAPLIAAFKTLGCRVSLFVDAGNPEIAQAAALGADRIELYTGPYAEAHHHGQSQPS
CCCHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHCCCCEEEEEECCCHHHHCCCCCCCC
LALFADAARRAHAAGLGINAGHDLSQHNLADFLAGVPDVLEVSIGHALVGEALYQGLEPT
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHH
VRAYLAIIAGGATTAA
HHHHHHHHCCCCCCCC
>Mature Secondary Structure 
STHLSVNVNKIAVLRNSRGGQDPDVVQAARSCIAAGAHGITVHPRPDQRHIRADDVYAL
CCEEEEECEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHH
STLTRMHGVEFNIEGNPFAPPRAGYPGLLELCRATRPQQVTLVPDGDGQLTSDHGVDFAR
HHHHHHHCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHC
DGARLAPLIAAFKTLGCRVSLFVDAGNPEIAQAAALGADRIELYTGPYAEAHHHGQSQPS
CCCHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHCCCCEEEEEECCCHHHHCCCCCCCC
LALFADAARRAHAAGLGINAGHDLSQHNLADFLAGVPDVLEVSIGHALVGEALYQGLEPT
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHH
VRAYLAIIAGGATTAA
HHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA