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The map label for this gene is rsuA [H]

Identifier: 66047208

GI number: 66047208

Start: 4734188

End: 4734880

Strand: Direct

Name: rsuA [H]

Synonym: Psyr_3981

Alternate gene names: 66047208

Gene position: 4734188-4734880 (Clockwise)

Preceding gene: 66047207

Following gene: 66047209

Centisome position: 77.69

GC content: 60.61

Gene sequence:

>693_bases
ATGCGCCTTGATCGTTTCCTCAGCAACCTGCCACGCTTCAATCGTCAGCAAGTGCGCCTGGCGCTGGCCAGCGGACGGGT
GCAGGTAGACGGCCAGGCAACCCGCGACCCGCACCACGAAGTACGCGAATTCAGTTGTGTGGCGCTTGACGAAGAAATCC
TCCAGGCAGGCAAAGTCCCCCGCTACTTCATGCTGCACAAGCCCGAAGGCTGCGTCAGCGCGACGAGCGATGCGCAGCAT
CCGACCGTGCTCGATTTGCTGGACGAGCCTGACAAGCACGAACTGCACATCGCCGGACGGCTGGACTTCAACACCACCGG
CCTGATGCTGATAACCAACGACGGTCAATGGTCGCGGCGCCTGACTCAGCCACAGAGCAAAATGCCCAAAGTCTATTACG
TCGAGACCGAGCAACCGATCGACGAACGCTACGCCGCGAAATTCGCCGAAGGCCTGTATTTCGCATTCGAAGGCATCACC
ACGCAACCGGCGCAATTGACCTTATTGGGGCCGCGCAGTGCGCGCCTGAGCATTGTCGAAGGCCGCTATCATCAGGTGAA
ACGAATGTTCGGCCACTTCGACAACAAAGTGTTGCGCCTGCATCGGGAAACCATCGGCGCGCTGCTGCTGGACCCCGACC
TGCGCCCCGGCAGCTACCGTGCGCTGAACGCCGGGGAAATCGGCCTGTTTTGA

Upstream 100 bases:

>100_bases
GCCCGCGCTGCGCCGGGATCAAGGATGCTGCGCCAAATCCCCAGGCACGCCGGGCCACAGAGTAAGATGCGCGCCCCGTC
CTCCGCTCGCCATTATCGCC

Downstream 100 bases:

>100_bases
ACGTCATAGCCGCCTGGCAGACGCTGGTCGGATCGGCACAGGCCTTTGATCGACTATCGCATGTCTGTCACGAAACGCGG
GCAATGTCATAGGCACGCAT

Product: pseudouridine synthase, Rsu

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MRLDRFLSNLPRFNRQQVRLALASGRVQVDGQATRDPHHEVREFSCVALDEEILQAGKVPRYFMLHKPEGCVSATSDAQH
PTVLDLLDEPDKHELHIAGRLDFNTTGLMLITNDGQWSRRLTQPQSKMPKVYYVETEQPIDERYAAKFAEGLYFAFEGIT
TQPAQLTLLGPRSARLSIVEGRYHQVKRMFGHFDNKVLRLHRETIGALLLDPDLRPGSYRALNAGEIGLF

Sequences:

>Translated_230_residues
MRLDRFLSNLPRFNRQQVRLALASGRVQVDGQATRDPHHEVREFSCVALDEEILQAGKVPRYFMLHKPEGCVSATSDAQH
PTVLDLLDEPDKHELHIAGRLDFNTTGLMLITNDGQWSRRLTQPQSKMPKVYYVETEQPIDERYAAKFAEGLYFAFEGIT
TQPAQLTLLGPRSARLSIVEGRYHQVKRMFGHFDNKVLRLHRETIGALLLDPDLRPGSYRALNAGEIGLF
>Mature_230_residues
MRLDRFLSNLPRFNRQQVRLALASGRVQVDGQATRDPHHEVREFSCVALDEEILQAGKVPRYFMLHKPEGCVSATSDAQH
PTVLDLLDEPDKHELHIAGRLDFNTTGLMLITNDGQWSRRLTQPQSKMPKVYYVETEQPIDERYAAKFAEGLYFAFEGIT
TQPAQLTLLGPRSARLSIVEGRYHQVKRMFGHFDNKVLRLHRETIGALLLDPDLRPGSYRALNAGEIGLF

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=228, Percent_Identity=46.4912280701754, Blast_Score=199, Evalue=9e-53,
Organism=Escherichia coli, GI1787524, Length=233, Percent_Identity=25.7510729613734, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1790453, Length=233, Percent_Identity=24.4635193133047, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26209; Mature: 26209

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDRFLSNLPRFNRQQVRLALASGRVQVDGQATRDPHHEVREFSCVALDEEILQAGKVP
CCHHHHHHHCCCCCHHHEEEEEECCEEEECCCCCCCHHHHHHHHHHEEECHHHHHHCCCC
RYFMLHKPEGCVSATSDAQHPTVLDLLDEPDKHELHIAGRLDFNTTGLMLITNDGQWSRR
EEEEEECCCCCCCCCCCCCCCEEEHHCCCCCCCEEEEEEEECCCCCEEEEEECCCCHHHH
LTQPQSKMPKVYYVETEQPIDERYAAKFAEGLYFAFEGITTQPAQLTLLGPRSARLSIVE
HCCCHHHCCEEEEEECCCCHHHHHHHHHHHCEEEEEECCCCCCCEEEEECCCCCEEEEEC
GRYHQVKRMFGHFDNKVLRLHRETIGALLLDPDLRPGSYRALNAGEIGLF
CHHHHHHHHHHHHCHHHHHHHHHHHCEEEECCCCCCCCEEEECCCCCCCC
>Mature Secondary Structure
MRLDRFLSNLPRFNRQQVRLALASGRVQVDGQATRDPHHEVREFSCVALDEEILQAGKVP
CCHHHHHHHCCCCCHHHEEEEEECCEEEECCCCCCCHHHHHHHHHHEEECHHHHHHCCCC
RYFMLHKPEGCVSATSDAQHPTVLDLLDEPDKHELHIAGRLDFNTTGLMLITNDGQWSRR
EEEEEECCCCCCCCCCCCCCCEEEHHCCCCCCCEEEEEEEECCCCCEEEEEECCCCHHHH
LTQPQSKMPKVYYVETEQPIDERYAAKFAEGLYFAFEGITTQPAQLTLLGPRSARLSIVE
HCCCHHHCCEEEEEECCCCHHHHHHHHHHHCEEEEEECCCCCCCEEEEECCCCCEEEEEC
GRYHQVKRMFGHFDNKVLRLHRETIGALLLDPDLRPGSYRALNAGEIGLF
CHHHHHHHHHHHHCHHHHHHHHHHHCEEEECCCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]