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The map label for this gene is amtB [H]

Identifier: 66045517

GI number: 66045517

Start: 2643672

End: 2644997

Strand: Direct

Name: amtB [H]

Synonym: Psyr_2277

Alternate gene names: 66045517

Gene position: 2643672-2644997 (Clockwise)

Preceding gene: 66045516

Following gene: 66045520

Centisome position: 43.38

GC content: 62.75

Gene sequence:

>1326_bases
ATGGTCAATCGTCTACACGGCAAATCCCTTACAGCCTTACTCGCGCTCGGCGCCTTTGCCCCGATTGCGCAGGCAGCTGA
CGCCCCCGTTCTGAACACCGGCAGCACGGCCTGGATGGTCACCGCAGCCGTGCTGGTGCTGTTCATGTGCCTTCCGGGGC
TGGCACTGTTTTACGGCGGTCTGGTACGGGCCAAGAACATGCTCTCGCTGTTCACCCAGTGTTTTGGCATTGCCGGAGTC
GTGGGCGTGCTGTGGGTCATCTATGGCTACAGCATGGTGGTCGACACCAGCAACATGGTCGAAGGACAAGTGAGTTTCAA
TAGTTTCGTGGGCGGTCTGAGTCGTGCGTTTCTGGCCGGCATGACCCCCGACAGCCTGGTCGGCGATATCCCTGAAGGGG
TATTCGTAACCTTCCAGATGACCTTCGCCATCATCACCCCGGCGCTGATTGCCGGGGCCTTCGCCGAACGCATGAAGTTC
TCTGCGGCGCTGCTGTTCATGGCGCTGTGGTTCACCCTGGTGTATGCGCCGGTCGCGCACATGGTCTGGGGGGGCGCCGG
CGCCTTGATGCACAACTGGGGCGTGCTGGACTTCGCTGGCGGCACCGCGGTGCACATCAATGCCGGTGTCGCGGCACTGG
CGGCATGTCTGGTACTGGGCAAGCGCAAGGGTTATCAGAACACCCCGATGCCGGCGCACAACCTGAGCCTGACCATGGCC
GGCGCAGCGATGCTCTGGGTCGGCTGGTTCGGTTTCAATATCGGTTCCGGTGGTGGCCTGAACGGCACGTCCGGCATCGT
CATGCTCAACACGCAAGTGGGGGCGTGCGCCGGGATACTCGGCTGGATGTTCACCGAGTGGTTCAAGGTCGGCAAACCCA
GCGCGCTGGGTCTGGCCAGCGGTGCATTGGCGGGTCTGGTCGGCATCACCCCGGCGTGTGCCTACGTCGGAGTCGGCGGG
GCACTGGCCATCGGCGTGCTGTGCGGAGTGTTCTGCTACCTGAGCGTGACCGTGCTGAAAAAACGCCTGGGCTATGACGA
CAGCCTCGACGTCTTCGGCCTGCACGGCATCGGCGGCATGATCGGTGCAATACTGACCGGCGTGTTCTGCGTACCCGCAC
TGGGCGGTCTGGTGCCCGATGTCAGCATGGGCGCCCAAGTGATTGCCCAGATCAAAGGCGTGCTGTTCACCACCGCCTAC
TGCTTCGCGGTCAGCTGGATCATCCTCAAAGCCGTGCAGGCACTGATCGGCCTGCGCGCTCACGAGTCAGTCGAAGAAAT
GGGCCTGGACCTGGCCGAGCACAACGAACGGGCCTACAACCATTGA

Upstream 100 bases:

>100_bases
CTCCAGGACGATGAACCCTCAAGAGGCCAAAGAGCCTCGCCTTTCTGAATAACCCTCTTTGCTGCCAGACACTTTCCATT
CATTTGCGGGAGCTTTGAAC

Downstream 100 bases:

>100_bases
GAGGGCTGCCGGCCAATGTCATGCCTTGCGCGAGGTCGCTGCATATACAAGCGCTTTTCTATCGCGATGGCACAGACGAC
GCAGAGTGCGACGTTAGTGA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MVNRLHGKSLTALLALGAFAPIAQAADAPVLNTGSTAWMVTAAVLVLFMCLPGLALFYGGLVRAKNMLSLFTQCFGIAGV
VGVLWVIYGYSMVVDTSNMVEGQVSFNSFVGGLSRAFLAGMTPDSLVGDIPEGVFVTFQMTFAIITPALIAGAFAERMKF
SAALLFMALWFTLVYAPVAHMVWGGAGALMHNWGVLDFAGGTAVHINAGVAALAACLVLGKRKGYQNTPMPAHNLSLTMA
GAAMLWVGWFGFNIGSGGGLNGTSGIVMLNTQVGACAGILGWMFTEWFKVGKPSALGLASGALAGLVGITPACAYVGVGG
ALAIGVLCGVFCYLSVTVLKKRLGYDDSLDVFGLHGIGGMIGAILTGVFCVPALGGLVPDVSMGAQVIAQIKGVLFTTAY
CFAVSWIILKAVQALIGLRAHESVEEMGLDLAEHNERAYNH

Sequences:

>Translated_441_residues
MVNRLHGKSLTALLALGAFAPIAQAADAPVLNTGSTAWMVTAAVLVLFMCLPGLALFYGGLVRAKNMLSLFTQCFGIAGV
VGVLWVIYGYSMVVDTSNMVEGQVSFNSFVGGLSRAFLAGMTPDSLVGDIPEGVFVTFQMTFAIITPALIAGAFAERMKF
SAALLFMALWFTLVYAPVAHMVWGGAGALMHNWGVLDFAGGTAVHINAGVAALAACLVLGKRKGYQNTPMPAHNLSLTMA
GAAMLWVGWFGFNIGSGGGLNGTSGIVMLNTQVGACAGILGWMFTEWFKVGKPSALGLASGALAGLVGITPACAYVGVGG
ALAIGVLCGVFCYLSVTVLKKRLGYDDSLDVFGLHGIGGMIGAILTGVFCVPALGGLVPDVSMGAQVIAQIKGVLFTTAY
CFAVSWIILKAVQALIGLRAHESVEEMGLDLAEHNERAYNH
>Mature_441_residues
MVNRLHGKSLTALLALGAFAPIAQAADAPVLNTGSTAWMVTAAVLVLFMCLPGLALFYGGLVRAKNMLSLFTQCFGIAGV
VGVLWVIYGYSMVVDTSNMVEGQVSFNSFVGGLSRAFLAGMTPDSLVGDIPEGVFVTFQMTFAIITPALIAGAFAERMKF
SAALLFMALWFTLVYAPVAHMVWGGAGALMHNWGVLDFAGGTAVHINAGVAALAACLVLGKRKGYQNTPMPAHNLSLTMA
GAAMLWVGWFGFNIGSGGGLNGTSGIVMLNTQVGACAGILGWMFTEWFKVGKPSALGLASGALAGLVGITPACAYVGVGG
ALAIGVLCGVFCYLSVTVLKKRLGYDDSLDVFGLHGIGGMIGAILTGVFCVPALGGLVPDVSMGAQVIAQIKGVLFTTAY
CFAVSWIILKAVQALIGLRAHESVEEMGLDLAEHNERAYNH

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=432, Percent_Identity=45.6018518518519, Blast_Score=350, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI32564042, Length=327, Percent_Identity=29.0519877675841, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI133931100, Length=444, Percent_Identity=25.9009009009009, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17550182, Length=446, Percent_Identity=27.1300448430493, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17550180, Length=445, Percent_Identity=24.9438202247191, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324187, Length=426, Percent_Identity=34.9765258215962, Blast_Score=200, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6325396, Length=425, Percent_Identity=32.7058823529412, Blast_Score=192, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6321559, Length=432, Percent_Identity=32.1759259259259, Blast_Score=182, Evalue=1e-46,
Organism=Drosophila melanogaster, GI161078314, Length=430, Percent_Identity=29.3023255813954, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 45722; Mature: 45722

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNRLHGKSLTALLALGAFAPIAQAADAPVLNTGSTAWMVTAAVLVLFMCLPGLALFYGG
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LVRAKNMLSLFTQCFGIAGVVGVLWVIYGYSMVVDTSNMVEGQVSFNSFVGGLSRAFLAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHCCCHHHHHHHHHHHHHHHHC
MTPDSLVGDIPEGVFVTFQMTFAIITPALIAGAFAERMKFSAALLFMALWFTLVYAPVAH
CCCHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVWGGAGALMHNWGVLDFAGGTAVHINAGVAALAACLVLGKRKGYQNTPMPAHNLSLTMA
HHHCCCHHHHHCCCCEEECCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHH
GAAMLWVGWFGFNIGSGGGLNGTSGIVMLNTQVGACAGILGWMFTEWFKVGKPSALGLAS
HHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHH
GALAGLVGITPACAYVGVGGALAIGVLCGVFCYLSVTVLKKRLGYDDSLDVFGLHGIGGM
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHH
IGAILTGVFCVPALGGLVPDVSMGAQVIAQIKGVLFTTAYCFAVSWIILKAVQALIGLRA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HESVEEMGLDLAEHNERAYNH
HHHHHHHCCCHHHCCCCCCCC
>Mature Secondary Structure
MVNRLHGKSLTALLALGAFAPIAQAADAPVLNTGSTAWMVTAAVLVLFMCLPGLALFYGG
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LVRAKNMLSLFTQCFGIAGVVGVLWVIYGYSMVVDTSNMVEGQVSFNSFVGGLSRAFLAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHCCCHHHHHHHHHHHHHHHHC
MTPDSLVGDIPEGVFVTFQMTFAIITPALIAGAFAERMKFSAALLFMALWFTLVYAPVAH
CCCHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVWGGAGALMHNWGVLDFAGGTAVHINAGVAALAACLVLGKRKGYQNTPMPAHNLSLTMA
HHHCCCHHHHHCCCCEEECCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHH
GAAMLWVGWFGFNIGSGGGLNGTSGIVMLNTQVGACAGILGWMFTEWFKVGKPSALGLAS
HHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHH
GALAGLVGITPACAYVGVGGALAIGVLCGVFCYLSVTVLKKRLGYDDSLDVFGLHGIGGM
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHH
IGAILTGVFCVPALGGLVPDVSMGAQVIAQIKGVLFTTAYCFAVSWIILKAVQALIGLRA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HESVEEMGLDLAEHNERAYNH
HHHHHHHCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]