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The map label for this gene is glnT [H]

Identifier: 66045513

GI number: 66045513

Start: 2638817

End: 2640151

Strand: Direct

Name: glnT [H]

Synonym: Psyr_2273

Alternate gene names: 66045513

Gene position: 2638817-2640151 (Clockwise)

Preceding gene: 66045511

Following gene: 66045514

Centisome position: 43.3

GC content: 60.15

Gene sequence:

>1335_bases
ATGTTGCCACCCGAAACACAGCGTCTGATCGAACAGCACGGCATCAAGTACGTACTGGCTCAGTTCGTCGACATTCATGG
TTCGGCCAAGACCAAATCGGTTCCTGTGACAGGTCTGGAAATGGTTGCAGAGGACGGTGCCGGCTTTGCCGGTTTCGCGA
TCTGCGGGATGGGCATGGAGCCTCATGGGCCGGACTTCATGGCCAGGGGTGATCTGTCGTCGCTGACCCCGGTGCCATGG
CAGCCGGGCTATGGACGGGTGGTGTGCATCGGCCATGTGGATGGCAAACCCTGGCCCTATGACAGCCGCTATGTGCTGCA
GCAGCAGGTCGAACGCCTCAGTCAGCGTGGCTGGAGCCTCAATACCGGCCTGGAGCCCGAGTTCAGCCTGTTCAAGCGCG
ATGTCACCGGCAGCCTGCAGATGGTCGATGCCAGCGATAACCTCGACAAGCCTTGCTACGACTACAAGGGGCTGTCGCGC
TCTCGCGAGTTTCTCGAGCGCCTGACCGAGGCATTGCAGCCGGTGGGTTTCGACATTTATCAGATCGATCATGAAGACGC
CAACGGCCAGTTCGAGATCAATTACACCTACAGCGAGGCCATGGAATCGGCTGACCGTTTCACCTTCTTCCGCATGGCCG
CCGGTGAGATCGCCAATGACATGGGCATGATCTGCTCGTTCATGCCCAAGCCCGATCCCAAACGGGCCGGCAATGGCATG
CACTTTCACTTGTCGATCGCCAGTGCCAGTAACAAGAACCTGTTCCACGATGCCAGCGATCCGAGCGGCATGGGGTTGTC
GACGCTGGCCTATCACTTCGCTGCGGGCCTGCTGGCTCACGGGCCTGCGCTGTGCGCCTTTGCGGCACCCACGGTCAACT
CCTACAAACGCCTGGTGGTCGGCAACTCACTGTCGGGCGCCACCTGGGCCCCGGCGTTTATTGCCTTCGGCGCCAACAAC
CGCTCGGCGATGGTGCGCGTGCCCTATGGCCGCCTGGAGTTTCGTCTGCCCGACGCCGGTTGCAATCCGTATCTGGTCAG
CGCCGCAATCATTGCCGCAGGCCTGGACGGTATCGACCGGCAACTGGAGATCGATCACGTCTGCAACGAGAACCTCTACA
AATTGAGCCTCGAAGAGATCGCCGCACGGGGCATCAAGACCCTGCCGCAGTCGCTCAACGAAGCCTGCGACGCGCTGCAA
GCCGACCCGTTGTTTGGCGAAGTGCTGGGCAGCGAGATTGTCGATGAATTCATCCGCCTCAAGCGCATGGAGTGGGTGGA
ATACAGCCGCCACGTGAGCGATTGGGAAGTGAAGCGCTATATCGAATTTTTCTAG

Upstream 100 bases:

>100_bases
CCTAAAGGGAAAGTAACTTTCCTGTTCGGAATATATCCCGAAAGGCAGTGAACAGCGGCCCTTCCCTGAAACGTTTTGCT
TTTGCCAGGAGACACGTCCC

Downstream 100 bases:

>100_bases
CGGCAAGCTTCAGAGAGCAGCTGCAAGTTTCAAGCGGCAAGCTGCAAGAGGAAGCTGGATTGCGGCGTATTTGATTTCTC
TGTCTATGCGGTTGGTTTTA

Product: glutamate--ammonia ligase

Products: NA

Alternate protein names: Glutamate--ammonia ligase; Glutamine synthetase III; GSIII [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MLPPETQRLIEQHGIKYVLAQFVDIHGSAKTKSVPVTGLEMVAEDGAGFAGFAICGMGMEPHGPDFMARGDLSSLTPVPW
QPGYGRVVCIGHVDGKPWPYDSRYVLQQQVERLSQRGWSLNTGLEPEFSLFKRDVTGSLQMVDASDNLDKPCYDYKGLSR
SREFLERLTEALQPVGFDIYQIDHEDANGQFEINYTYSEAMESADRFTFFRMAAGEIANDMGMICSFMPKPDPKRAGNGM
HFHLSIASASNKNLFHDASDPSGMGLSTLAYHFAAGLLAHGPALCAFAAPTVNSYKRLVVGNSLSGATWAPAFIAFGANN
RSAMVRVPYGRLEFRLPDAGCNPYLVSAAIIAAGLDGIDRQLEIDHVCNENLYKLSLEEIAARGIKTLPQSLNEACDALQ
ADPLFGEVLGSEIVDEFIRLKRMEWVEYSRHVSDWEVKRYIEFF

Sequences:

>Translated_444_residues
MLPPETQRLIEQHGIKYVLAQFVDIHGSAKTKSVPVTGLEMVAEDGAGFAGFAICGMGMEPHGPDFMARGDLSSLTPVPW
QPGYGRVVCIGHVDGKPWPYDSRYVLQQQVERLSQRGWSLNTGLEPEFSLFKRDVTGSLQMVDASDNLDKPCYDYKGLSR
SREFLERLTEALQPVGFDIYQIDHEDANGQFEINYTYSEAMESADRFTFFRMAAGEIANDMGMICSFMPKPDPKRAGNGM
HFHLSIASASNKNLFHDASDPSGMGLSTLAYHFAAGLLAHGPALCAFAAPTVNSYKRLVVGNSLSGATWAPAFIAFGANN
RSAMVRVPYGRLEFRLPDAGCNPYLVSAAIIAAGLDGIDRQLEIDHVCNENLYKLSLEEIAARGIKTLPQSLNEACDALQ
ADPLFGEVLGSEIVDEFIRLKRMEWVEYSRHVSDWEVKRYIEFF
>Mature_444_residues
MLPPETQRLIEQHGIKYVLAQFVDIHGSAKTKSVPVTGLEMVAEDGAGFAGFAICGMGMEPHGPDFMARGDLSSLTPVPW
QPGYGRVVCIGHVDGKPWPYDSRYVLQQQVERLSQRGWSLNTGLEPEFSLFKRDVTGSLQMVDASDNLDKPCYDYKGLSR
SREFLERLTEALQPVGFDIYQIDHEDANGQFEINYTYSEAMESADRFTFFRMAAGEIANDMGMICSFMPKPDPKRAGNGM
HFHLSIASASNKNLFHDASDPSGMGLSTLAYHFAAGLLAHGPALCAFAAPTVNSYKRLVVGNSLSGATWAPAFIAFGANN
RSAMVRVPYGRLEFRLPDAGCNPYLVSAAIIAAGLDGIDRQLEIDHVCNENLYKLSLEEIAARGIKTLPQSLNEACDALQ
ADPLFGEVLGSEIVDEFIRLKRMEWVEYSRHVSDWEVKRYIEFF

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family. Type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=442, Percent_Identity=23.7556561085973, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI1790301, Length=473, Percent_Identity=28.7526427061311, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI87081870, Length=464, Percent_Identity=28.0172413793103, Blast_Score=141, Evalue=1e-34,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR017536 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 49134; Mature: 49134

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPPETQRLIEQHGIKYVLAQFVDIHGSAKTKSVPVTGLEMVAEDGAGFAGFAICGMGME
CCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCEEHHHHCCCCCC
PHGPDFMARGDLSSLTPVPWQPGYGRVVCIGHVDGKPWPYDSRYVLQQQVERLSQRGWSL
CCCCCCEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCC
NTGLEPEFSLFKRDVTGSLQMVDASDNLDKPCYDYKGLSRSREFLERLTEALQPVGFDIY
CCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCEEE
QIDHEDANGQFEINYTYSEAMESADRFTFFRMAAGEIANDMGMICSFMPKPDPKRAGNGM
EEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCE
HFHLSIASASNKNLFHDASDPSGMGLSTLAYHFAAGLLAHGPALCAFAAPTVNSYKRLVV
EEEEEEECCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCHHEEECCCCHHCCCEEEE
GNSLSGATWAPAFIAFGANNRSAMVRVPYGRLEFRLPDAGCNPYLVSAAIIAAGLDGIDR
CCCCCCCCCCCEEEEECCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCC
QLEIDHVCNENLYKLSLEEIAARGIKTLPQSLNEACDALQADPLFGEVLGSEIVDEFIRL
EEEHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
KRMEWVEYSRHVSDWEVKRYIEFF
HHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MLPPETQRLIEQHGIKYVLAQFVDIHGSAKTKSVPVTGLEMVAEDGAGFAGFAICGMGME
CCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCEEHHHHCCCCCC
PHGPDFMARGDLSSLTPVPWQPGYGRVVCIGHVDGKPWPYDSRYVLQQQVERLSQRGWSL
CCCCCCEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCC
NTGLEPEFSLFKRDVTGSLQMVDASDNLDKPCYDYKGLSRSREFLERLTEALQPVGFDIY
CCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCEEE
QIDHEDANGQFEINYTYSEAMESADRFTFFRMAAGEIANDMGMICSFMPKPDPKRAGNGM
EEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCE
HFHLSIASASNKNLFHDASDPSGMGLSTLAYHFAAGLLAHGPALCAFAAPTVNSYKRLVV
EEEEEEECCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCHHEEECCCCHHCCCEEEE
GNSLSGATWAPAFIAFGANNRSAMVRVPYGRLEFRLPDAGCNPYLVSAAIIAAGLDGIDR
CCCCCCCCCCCEEEEECCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCC
QLEIDHVCNENLYKLSLEEIAARGIKTLPQSLNEACDALQADPLFGEVLGSEIVDEFIRL
EEEHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
KRMEWVEYSRHVSDWEVKRYIEFF
HHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1356885 [H]