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The map label for this gene is pheS

Identifier: 66045406

GI number: 66045406

Start: 2518954

End: 2519970

Strand: Direct

Name: pheS

Synonym: Psyr_2166

Alternate gene names: 66045406

Gene position: 2518954-2519970 (Clockwise)

Preceding gene: 66045405

Following gene: 66045407

Centisome position: 41.34

GC content: 58.21

Gene sequence:

>1017_bases
ATGGAAAACCTGGACGCGCTGGTCTCTCAAGCTCTTGAGGCTGTGCAAAGCGCCGAAGATATCAATGCCCTGGAGCAAAT
CCGGGTTCACTACCTCGGCAAGAAAGGCGAGTTGACTCAGGTGATGAAGACCCTGGGGAACCTGCCAGCTGAAGAGCGTC
CGCAAGTCGGTGCGCTGATCAACGTTGCCAAGGAACGTGTCACAGAGGTTCTCAATGCACGCAAGGCATCGTTTGAGCAG
GCAGAACTGACTGCCAGGCTCGCGGCCGAATGCATCGATGTGACCCTGCCGGGCCGCGGTCAGACCTCAGGTGGTCTGCA
CCCGATTACCCGTACTCTGGAACGTATCGAACAATTCTTCACGCACATCGGCTACGGCATCGCCGAAGGCCCTGAAGTGG
AAGACGATTACCACAACTTCGAGGCGCTCAACATCCCAGGCCACCACCCGGCCCGGTCGATGCACGACACCTTCTATTTC
AACGCGAACATGCTGCTGCGCACTCACACCTCGCCGGTACAGGTACGCACCATGGAATCGCAGCAGCCGCCGATCCGCAT
CGTCTGCCCAGGCCGTGTGTACCGTAGCGACTCGGATATCACCCACTCGCCGATGTTCCACCAGATCGAAGGTCTGCTGG
TCGATCGCGACATCAATTTTGCCGATCTGAAAGGCACCATCGAAGAGTTCCTGCGTGTGTTCTTCGAGAAAGAGCTGGCC
GTGCGCTTCCGTCCTTCGTATTTCCCGTTCACCGAGCCCTCGGCGGAAGTGGATATGGAATGCGTGATGTGCAGTGGCAA
GGGCTGCCGCGTCTGCAAGCAGACCGGCTGGCTTGAAGTCATGGGGTGCGGCATGGTCCACCCGAACGTGCTGCGCATGT
CCGGCATCGATCCTGAAGAGTTTCAGGGCTTTGCGTTCGGCATGGGCGTAGAGCGTCTGGCCATGCTGCGTTACGGCGTC
AATGATTTGCGCCTGTTCTTCGACAACGATTTGCGGTTCCTCGCGCAATTTCGCTAA

Upstream 100 bases:

>100_bases
CCGACAATCACTTGCCCCGGTTCTCCGGGGTCGGTGTTAAACGTCATAAATAGGGGAAGAGCCTTCAGCTCTTCCCCTAT
TTTGTATCTGGAGTCTGTAC

Downstream 100 bases:

>100_bases
GTCGTACGAACCCATCAGGAGAGCAGGATGAAATTCAGTGAACAATGGCTGCGCGGCTGGGTAAGCCCGCAGGTTTCCCG
CGACGAGCTGGTTGCTCGTC

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MENLDALVSQALEAVQSAEDINALEQIRVHYLGKKGELTQVMKTLGNLPAEERPQVGALINVAKERVTEVLNARKASFEQ
AELTARLAAECIDVTLPGRGQTSGGLHPITRTLERIEQFFTHIGYGIAEGPEVEDDYHNFEALNIPGHHPARSMHDTFYF
NANMLLRTHTSPVQVRTMESQQPPIRIVCPGRVYRSDSDITHSPMFHQIEGLLVDRDINFADLKGTIEEFLRVFFEKELA
VRFRPSYFPFTEPSAEVDMECVMCSGKGCRVCKQTGWLEVMGCGMVHPNVLRMSGIDPEEFQGFAFGMGVERLAMLRYGV
NDLRLFFDNDLRFLAQFR

Sequences:

>Translated_338_residues
MENLDALVSQALEAVQSAEDINALEQIRVHYLGKKGELTQVMKTLGNLPAEERPQVGALINVAKERVTEVLNARKASFEQ
AELTARLAAECIDVTLPGRGQTSGGLHPITRTLERIEQFFTHIGYGIAEGPEVEDDYHNFEALNIPGHHPARSMHDTFYF
NANMLLRTHTSPVQVRTMESQQPPIRIVCPGRVYRSDSDITHSPMFHQIEGLLVDRDINFADLKGTIEEFLRVFFEKELA
VRFRPSYFPFTEPSAEVDMECVMCSGKGCRVCKQTGWLEVMGCGMVHPNVLRMSGIDPEEFQGFAFGMGVERLAMLRYGV
NDLRLFFDNDLRFLAQFR
>Mature_338_residues
MENLDALVSQALEAVQSAEDINALEQIRVHYLGKKGELTQVMKTLGNLPAEERPQVGALINVAKERVTEVLNARKASFEQ
AELTARLAAECIDVTLPGRGQTSGGLHPITRTLERIEQFFTHIGYGIAEGPEVEDDYHNFEALNIPGHHPARSMHDTFYF
NANMLLRTHTSPVQVRTMESQQPPIRIVCPGRVYRSDSDITHSPMFHQIEGLLVDRDINFADLKGTIEEFLRVFFEKELA
VRFRPSYFPFTEPSAEVDMECVMCSGKGCRVCKQTGWLEVMGCGMVHPNVLRMSGIDPEEFQGFAFGMGVERLAMLRYGV
NDLRLFFDNDLRFLAQFR

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=316, Percent_Identity=30.0632911392405, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI4758340, Length=270, Percent_Identity=30.3703703703704, Blast_Score=118, Evalue=9e-27,
Organism=Escherichia coli, GI1788007, Length=338, Percent_Identity=64.4970414201183, Blast_Score=461, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI32566635, Length=249, Percent_Identity=34.136546184739, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI32563657, Length=275, Percent_Identity=29.0909090909091, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17508957, Length=275, Percent_Identity=28.7272727272727, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321087, Length=270, Percent_Identity=29.2592592592593, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6325304, Length=279, Percent_Identity=29.0322580645161, Blast_Score=103, Evalue=4e-23,
Organism=Drosophila melanogaster, GI18858079, Length=274, Percent_Identity=29.9270072992701, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI17137424, Length=290, Percent_Identity=28.6206896551724, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_PSE14 (Q48JR9)

Other databases:

- EMBL:   CP000058
- RefSeq:   YP_274358.1
- ProteinModelPortal:   Q48JR9
- SMR:   Q48JR9
- STRING:   Q48JR9
- GeneID:   3559035
- GenomeReviews:   CP000058_GR
- KEGG:   psp:PSPPH_2139
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- PhylomeDB:   Q48JR9
- ProtClustDB:   PRK00488
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 38199; Mature: 38199

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENLDALVSQALEAVQSAEDINALEQIRVHYLGKKGELTQVMKTLGNLPAEERPQVGALI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHH
NVAKERVTEVLNARKASFEQAELTARLAAECIDVTLPGRGQTSGGLHPITRTLERIEQFF
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHH
THIGYGIAEGPEVEDDYHNFEALNIPGHHPARSMHDTFYFNANMLLRTHTSPVQVRTMES
HHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEEECEEEEEECCCCEEEEEECC
QQPPIRIVCPGRVYRSDSDITHSPMFHQIEGLLVDRDINFADLKGTIEEFLRVFFEKELA
CCCCEEEECCCCEECCCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
VRFRPSYFPFTEPSAEVDMECVMCSGKGCRVCKQTGWLEVMGCGMVHPNVLRMSGIDPEE
EEECCCCCCCCCCCCCCCEEEEEECCCCCHHHHHCCCEEEEECCCCCCCEEEECCCCHHH
FQGFAFGMGVERLAMLRYGVNDLRLFFDNDLRFLAQFR
HCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCC
>Mature Secondary Structure
MENLDALVSQALEAVQSAEDINALEQIRVHYLGKKGELTQVMKTLGNLPAEERPQVGALI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHH
NVAKERVTEVLNARKASFEQAELTARLAAECIDVTLPGRGQTSGGLHPITRTLERIEQFF
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHH
THIGYGIAEGPEVEDDYHNFEALNIPGHHPARSMHDTFYFNANMLLRTHTSPVQVRTMES
HHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEEECEEEEEECCCCEEEEEECC
QQPPIRIVCPGRVYRSDSDITHSPMFHQIEGLLVDRDINFADLKGTIEEFLRVFFEKELA
CCCCEEEECCCCEECCCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
VRFRPSYFPFTEPSAEVDMECVMCSGKGCRVCKQTGWLEVMGCGMVHPNVLRMSGIDPEE
EEECCCCCCCCCCCCCCCEEEEEECCCCCHHHHHCCCEEEEECCCCCCCEEEECCCCHHH
FQGFAFGMGVERLAMLRYGVNDLRLFFDNDLRFLAQFR
HCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA