Click here to switch to the map view.

The map label for this gene is rihA [H]

Identifier: 66045397

GI number: 66045397

Start: 2512612

End: 2513637

Strand: Direct

Name: rihA [H]

Synonym: Psyr_2157

Alternate gene names: 66045397

Gene position: 2512612-2513637 (Clockwise)

Preceding gene: 66045396

Following gene: 66045399

Centisome position: 41.23

GC content: 59.84

Gene sequence:

>1026_bases
ATGACATTGATACAGTTTTCCAGACAGTTCATTCGTGGAGCGTTGCTCTTGTCCATTCTCGGTACTGCTGCTGTCCAGGC
GGCTGAAAAGCGTGATCTGATCATCGATACGGATCCAGGTGCGGACGATGTCGTTGCACTGTTACTGGCGCTGGCGTCAC
CTGAAGAGCTCAACGTGATGGCTATCACCACGGTGGCCGGCAATGTGCGGCTCGACAAGACCTCGCGTAATGCCAGGCTG
GCGCGTGAGTGGGCCGGGCGCGAAGAGGTGCCTGTGTATGCCGGTGCGCCCAAGCCGTTGGTGCGTACCCCGATCTATGC
CGAGAACGTTCACGGTCAGGAAGGTCTGCCGGGCGTGCCCGTGCACGAGCCCGCCAAGGGCCTTGCCGAAGGCAATGCCG
TCGATTACCTGATCCGCACCCTGAGCAAGGCCAAGCCGCATAGCATCACCATCGCCATGCTCGGCCCGCAGACCAACCTG
GCGCTGGCCTTGGTACAGGCTCCGGAAATCACTCAGGGCATCAAGGAAGTGGTGGTCATGGGTGGCGCGCATTTCAACGG
CGGCAATATCACCCCGGTTGCCGAATTCAATCTGTTCGCCGACCCGCATGCCGCGCAGATCGTTCTGGCCAGTGGTGTGA
AGCTGACGTATGTGCCGCTGGATGTGACCCACAAGATTCTCACCAGTGAACAACGCCTCAAGCAGATCGCCGCACTGGGC
AACAATGCTGGCAAGCTGGTCGACGGCATTCTCAATGAATACGTCAAGCTGGACATGGAGCATTACGGTTTGCCCGGCGG
GCCGGTACACGACGCCAGCGTGATTGCCTGGCTGCTCAAGCCCGAGCTGTTTTCCGGGCGGCAGATCAACGTGACAGTGG
ACACGCGTGAGGGCATCGGTTTTGGTCAGACAGTGGCCGACTGGTACGGCACGCTCAAGCAGCCGCAGAATGTATTCTGG
GTCGAGAACGGCGACGCTCAGGGGTTCTTCGATCTGCTGACCGAGCGTCTGGCGCGTCTCAAGTAA

Upstream 100 bases:

>100_bases
AGGCCAAGGCAATCATCCGTACCGGAGAATGTCAGCCGTACAGTAATGTGGCGCTGGTATCCGGAGTCGTCTTCTAATAA
TTCAATAAAGGGAGTTTCAT

Downstream 100 bases:

>100_bases
GTACGCAACCTGAGGCCGCGTATCAGGCGGGCAGTCCGGCCGCGGCATAGCGGCTCAGCACCTGGCTGATGAAGGCACGG
GCACCCTCGGTGCCCAGTTC

Product: inosine/uridine-preferring nucleoside hydrolase

Products: cytosine; ribose; uracil [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MTLIQFSRQFIRGALLLSILGTAAVQAAEKRDLIIDTDPGADDVVALLLALASPEELNVMAITTVAGNVRLDKTSRNARL
AREWAGREEVPVYAGAPKPLVRTPIYAENVHGQEGLPGVPVHEPAKGLAEGNAVDYLIRTLSKAKPHSITIAMLGPQTNL
ALALVQAPEITQGIKEVVVMGGAHFNGGNITPVAEFNLFADPHAAQIVLASGVKLTYVPLDVTHKILTSEQRLKQIAALG
NNAGKLVDGILNEYVKLDMEHYGLPGGPVHDASVIAWLLKPELFSGRQINVTVDTREGIGFGQTVADWYGTLKQPQNVFW
VENGDAQGFFDLLTERLARLK

Sequences:

>Translated_341_residues
MTLIQFSRQFIRGALLLSILGTAAVQAAEKRDLIIDTDPGADDVVALLLALASPEELNVMAITTVAGNVRLDKTSRNARL
AREWAGREEVPVYAGAPKPLVRTPIYAENVHGQEGLPGVPVHEPAKGLAEGNAVDYLIRTLSKAKPHSITIAMLGPQTNL
ALALVQAPEITQGIKEVVVMGGAHFNGGNITPVAEFNLFADPHAAQIVLASGVKLTYVPLDVTHKILTSEQRLKQIAALG
NNAGKLVDGILNEYVKLDMEHYGLPGGPVHDASVIAWLLKPELFSGRQINVTVDTREGIGFGQTVADWYGTLKQPQNVFW
VENGDAQGFFDLLTERLARLK
>Mature_340_residues
TLIQFSRQFIRGALLLSILGTAAVQAAEKRDLIIDTDPGADDVVALLLALASPEELNVMAITTVAGNVRLDKTSRNARLA
REWAGREEVPVYAGAPKPLVRTPIYAENVHGQEGLPGVPVHEPAKGLAEGNAVDYLIRTLSKAKPHSITIAMLGPQTNLA
LALVQAPEITQGIKEVVVMGGAHFNGGNITPVAEFNLFADPHAAQIVLASGVKLTYVPLDVTHKILTSEQRLKQIAALGN
NAGKLVDGILNEYVKLDMEHYGLPGGPVHDASVIAWLLKPELFSGRQINVTVDTREGIGFGQTVADWYGTLKQPQNVFWV
ENGDAQGFFDLLTERLARLK

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788486, Length=308, Percent_Identity=42.2077922077922, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1786871, Length=306, Percent_Identity=42.156862745098, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI1786213, Length=311, Percent_Identity=38.5852090032154, Blast_Score=171, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI32565474, Length=202, Percent_Identity=40.5940594059406, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17565698, Length=190, Percent_Identity=33.1578947368421, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320608, Length=262, Percent_Identity=31.2977099236641, Blast_Score=103, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24641837, Length=333, Percent_Identity=30.03003003003, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24641839, Length=193, Percent_Identity=35.2331606217617, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24583915, Length=193, Percent_Identity=32.1243523316062, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 36708; Mature: 36577

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLIQFSRQFIRGALLLSILGTAAVQAAEKRDLIIDTDPGADDVVALLLALASPEELNVM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCCCCEEE
AITTVAGNVRLDKTSRNARLAREWAGREEVPVYAGAPKPLVRTPIYAENVHGQEGLPGVP
EEEEEECCEEEECCCCCHHHHHHHCCCCCCCEEECCCCCHHCCCCEECCCCCCCCCCCCC
VHEPAKGLAEGNAVDYLIRTLSKAKPHSITIAMLGPQTNLALALVQAPEITQGIKEVVVM
CCCCCHHHCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEECCHHHHHHHHHHHC
GGAHFNGGNITPVAEFNLFADPHAAQIVLASGVKLTYVPLDVTHKILTSEQRLKQIAALG
CCCEECCCCCCEEEEEEEECCCCHHEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHC
NNAGKLVDGILNEYVKLDMEHYGLPGGPVHDASVIAWLLKPELFSGRQINVTVDTREGIG
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCHHHCCCCEEEEEEECCCCCC
FGQTVADWYGTLKQPQNVFWVENGDAQGFFDLLTERLARLK
CCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TLIQFSRQFIRGALLLSILGTAAVQAAEKRDLIIDTDPGADDVVALLLALASPEELNVM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCCCCEEE
AITTVAGNVRLDKTSRNARLAREWAGREEVPVYAGAPKPLVRTPIYAENVHGQEGLPGVP
EEEEEECCEEEECCCCCHHHHHHHCCCCCCCEEECCCCCHHCCCCEECCCCCCCCCCCCC
VHEPAKGLAEGNAVDYLIRTLSKAKPHSITIAMLGPQTNLALALVQAPEITQGIKEVVVM
CCCCCHHHCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEECCHHHHHHHHHHHC
GGAHFNGGNITPVAEFNLFADPHAAQIVLASGVKLTYVPLDVTHKILTSEQRLKQIAALG
CCCEECCCCCCEEEEEEEECCCCHHEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHC
NNAGKLVDGILNEYVKLDMEHYGLPGGPVHDASVIAWLLKPELFSGRQINVTVDTREGIG
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCHHHCCCCEEEEEEECCCCCC
FGQTVADWYGTLKQPQNVFWVENGDAQGFFDLLTERLARLK
CCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: cytidine; H2O; uridine [C]

Specific reaction: cytidine + H2O = cytosine + ribose uridine + H2O = uracil + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA