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The map label for this gene is rbsB [H]

Identifier: 66045391

GI number: 66045391

Start: 2506663

End: 2507622

Strand: Direct

Name: rbsB [H]

Synonym: Psyr_2151

Alternate gene names: 66045391

Gene position: 2506663-2507622 (Clockwise)

Preceding gene: 66045390

Following gene: 66045392

Centisome position: 41.14

GC content: 58.75

Gene sequence:

>960_bases
ATGAAGCTGCCATTCGCTGGACGCCTTCTCGCTGTCGCCATGTTTGCTGCCGTTACGGCAGTCATGCCCCTCTCTGCGGC
TTACGCCCAGACGCCTGAAAAACCCAAGGTCGCACTGGTCATGAAGTCGTTGGCCAACGAGTTCTTCCTGACCATGGAAG
ACGGTGCCAAGGCCTACCAGAAAGAACATGCCACCGAATTCGACCTGATCTCCAACGGGATCAAGGATGAGTCCGACACG
TCGGCGCAGATCCGCATCGTCGAACAGATGATCGCCTCCAACGTGAATGCGCTGGTGATCGCGCCAGCCGACTCCAAGGC
GCTGGTGCCGGTGCTCAAGAAAGCCACCGATGCCGGAATCAAGGTCGTCAACATCGACAACCAGCTCGATGTCGACGTGC
TCAAAAGCAAGAACCTGCAAATCCCCTTCGTAGGGCCTGACAACCGCAAGGGTGCCAAGCTGGTGGGCGACTATCTGGCC
GGGAAACTGACCGCTGGCGACGAGGTGGGCATCATCGAAGGGGTGTCGACCACCAACAATGCTCAGCAACGCACCGCCGG
GTTCAAGGACGCCATGGACGAAGCGAAAATGAAAGTGGTGTCCACGCAGTCCGGTAACTGGGAAATCGACAAGGGCAACG
CTGTGGCCTCGGCGATGCTCAACGAATACCCGAACCTGAAAGCCTTGCTGGCCGGTAATGACAGCATGGCGCTGGGCGCC
GTCTCTGCAGTGCGTGCGGCAGGCAAGGCTGGCAAGGTCATGGTTGTGGGCTACGACAACATCAATGCGATCAAGCCGAT
GCTCAAGGATGGCCGGGTTCTGGCCACGGCCGATCAGTACGCAGCCAAGCAGGCGGTCTTCGGCATCGAAGCTGCGCTCA
AGCTGGTCAAAGGCGAGAAGGTCGATACCAACGACAAGGGCGTGATCGAAACGCCGGTACAGCTCGTTACTCAACCTTGA

Upstream 100 bases:

>100_bases
TTTTCCATAGTTCGCTCCTCGCAAACGTTTGCGCGACGATGCCCGGCATCGATTCTGCGCCGTTCGCCCGAACAATAATC
ACAAGATCGGAGATGAACTC

Downstream 100 bases:

>100_bases
TCCACACCGTGTAAAGGTGCCCGTCCGCAGAGTACGGGCGCTGGGAGAAATGTATGTCAGCGTCTGCTCAGACAGCTGTT
CTTTCGGTCAGTGGCATCGG

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKLPFAGRLLAVAMFAAVTAVMPLSAAYAQTPEKPKVALVMKSLANEFFLTMEDGAKAYQKEHATEFDLISNGIKDESDT
SAQIRIVEQMIASNVNALVIAPADSKALVPVLKKATDAGIKVVNIDNQLDVDVLKSKNLQIPFVGPDNRKGAKLVGDYLA
GKLTAGDEVGIIEGVSTTNNAQQRTAGFKDAMDEAKMKVVSTQSGNWEIDKGNAVASAMLNEYPNLKALLAGNDSMALGA
VSAVRAAGKAGKVMVVGYDNINAIKPMLKDGRVLATADQYAAKQAVFGIEAALKLVKGEKVDTNDKGVIETPVQLVTQP

Sequences:

>Translated_319_residues
MKLPFAGRLLAVAMFAAVTAVMPLSAAYAQTPEKPKVALVMKSLANEFFLTMEDGAKAYQKEHATEFDLISNGIKDESDT
SAQIRIVEQMIASNVNALVIAPADSKALVPVLKKATDAGIKVVNIDNQLDVDVLKSKNLQIPFVGPDNRKGAKLVGDYLA
GKLTAGDEVGIIEGVSTTNNAQQRTAGFKDAMDEAKMKVVSTQSGNWEIDKGNAVASAMLNEYPNLKALLAGNDSMALGA
VSAVRAAGKAGKVMVVGYDNINAIKPMLKDGRVLATADQYAAKQAVFGIEAALKLVKGEKVDTNDKGVIETPVQLVTQP
>Mature_319_residues
MKLPFAGRLLAVAMFAAVTAVMPLSAAYAQTPEKPKVALVMKSLANEFFLTMEDGAKAYQKEHATEFDLISNGIKDESDT
SAQIRIVEQMIASNVNALVIAPADSKALVPVLKKATDAGIKVVNIDNQLDVDVLKSKNLQIPFVGPDNRKGAKLVGDYLA
GKLTAGDEVGIIEGVSTTNNAQQRTAGFKDAMDEAKMKVVSTQSGNWEIDKGNAVASAMLNEYPNLKALLAGNDSMALGA
VSAVRAAGKAGKVMVVGYDNINAIKPMLKDGRVLATADQYAAKQAVFGIEAALKLVKGEKVDTNDKGVIETPVQLVTQP

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=285, Percent_Identity=32.6315789473684, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1790526, Length=275, Percent_Identity=33.4545454545455, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1790674, Length=204, Percent_Identity=33.8235294117647, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788898, Length=307, Percent_Identity=25.0814332247557, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 33690; Mature: 33690

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPFAGRLLAVAMFAAVTAVMPLSAAYAQTPEKPKVALVMKSLANEFFLTMEDGAKAYQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEECCCHHHHH
KEHATEFDLISNGIKDESDTSAQIRIVEQMIASNVNALVIAPADSKALVPVLKKATDAGI
HHCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCE
KVVNIDNQLDVDVLKSKNLQIPFVGPDNRKGAKLVGDYLAGKLTAGDEVGIIEGVSTTNN
EEEECCCCCCHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCC
AQQRTAGFKDAMDEAKMKVVSTQSGNWEIDKGNAVASAMLNEYPNLKALLAGNDSMALGA
HHHHHCCHHHHHHHHHEEEEEECCCCEEEECCCHHHHHHHHCCCCEEEEEECCCCHHHHH
VSAVRAAGKAGKVMVVGYDNINAIKPMLKDGRVLATADQYAAKQAVFGIEAALKLVKGEK
HHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCC
VDTNDKGVIETPVQLVTQP
CCCCCCCEEECCHHHHCCC
>Mature Secondary Structure
MKLPFAGRLLAVAMFAAVTAVMPLSAAYAQTPEKPKVALVMKSLANEFFLTMEDGAKAYQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEECCCHHHHH
KEHATEFDLISNGIKDESDTSAQIRIVEQMIASNVNALVIAPADSKALVPVLKKATDAGI
HHCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCE
KVVNIDNQLDVDVLKSKNLQIPFVGPDNRKGAKLVGDYLAGKLTAGDEVGIIEGVSTTNN
EEEECCCCCCHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCC
AQQRTAGFKDAMDEAKMKVVSTQSGNWEIDKGNAVASAMLNEYPNLKALLAGNDSMALGA
HHHHHCCHHHHHHHHHEEEEEECCCCEEEECCCHHHHHHHHCCCCEEEEEECCCCHHHHH
VSAVRAAGKAGKVMVVGYDNINAIKPMLKDGRVLATADQYAAKQAVFGIEAALKLVKGEK
HHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCC
VDTNDKGVIETPVQLVTQP
CCCCCCCEEECCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6313683; 7686882; 9278503; 3011793; 3011794; 8899705; 9298646; 1583688; 7982928; 9641984 [H]