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The map label for this gene is mltF [H]

Identifier: 66045301

GI number: 66045301

Start: 2407822

End: 2409243

Strand: Direct

Name: mltF [H]

Synonym: Psyr_2061

Alternate gene names: 66045301

Gene position: 2407822-2409243 (Clockwise)

Preceding gene: 66045297

Following gene: 66045302

Centisome position: 39.51

GC content: 61.32

Gene sequence:

>1422_bases
ATGATCCGATCCGCGCTTGTGTCTTTGATCTGCCTGTTACCGCTGCTGCCCTTGCAGGTTTCTGCTCGGCAGACCGGTCC
CGAGGTCGTGCAGCACACGGTGCAGGTGCGCGATTTGCCGGCGATTCGCAGCAGCAAGGTGCTGCGCGTGCTGGTCAATC
AGAGCCGTAACAGCTCCGGCGACGTCAAGGGGCAGGAAATCGGCGTCGAATACCATCGTCTGCAAGCTTTCGAGCAGTAC
CTCAACAGCCATTCCCGTGCCGGGCAGAAGGTCACGATCAAGGTCATCCCCAAGGCCAAGAACCAGCTGCTGACGGCCTT
GCAGCGCGGAGAAGGCGACATGATCGCGCCCGGCGAGCTGCTCGATGTCAGCGACGCCAAGGGCATTCAGGCCAGCGCAC
CGATCGTGCACGACGTGCCTCTGGTGCTGGTCGGCGTTCGTGGTCAGCGCAGTGTGCGCCGTGTGGATCAACTGTCCGGA
AAGACCCTGAGCCTGCCCACCGGCAGTGCCGCCGATGAAGCCCTGCATCAGGTCAATCGACAACTGGAGCTGCGCAAGCT
GCCCTTGGCGAAAATCGAATGGGTCGACCCCAGTCTGGCCGTCGAGGATGTGCTGGAAATGGTTCAGGCCGGGATTTACC
CGATGACGCTGGTGGAGCAGCCGATTGCCGAGCGCTGGGCGAGGATCATGCCCAAACTGCGCATCGAACGCGGCCTGACC
TTGCAGACCCACGGGGATATCAGCTGGTTCGTTCGTGACAATGCCACCCAGCTGCGGGCCAGTGTCGACGATTTCCTCAA
AGACTATAAGCCGTCGGCCCAGCAGGATCAGGTGTTCGCCAACGCGTACAAGAACACCTACAAGGTCAACAACCCGTTGG
TGCGCAATAACCTGCAGCGTCTGGAACAACTGCGTCCGATGTTGCAACGCCATGCCGATGCACAGGGCATGGATTGGCTG
GACTTGGCTGCCCTCGCGTTCAAGGAATCCAAACTCGACCCGTCTGCCAAGGGCAGTGGCGGCGCGACAGGCCTGTTGCA
GATCACGCCTTCGGCAGCAAAAAGCGTTGGCGTGCCGAGCATCCAGAGCGCTGACGACAACATCCGTGCCGGGTCGCGCT
ACATGGCGCTGATCCAACGCAAGTATTTCTCCAGTAACCGGGTCAACGAACGCGAGCGCATGGCATTCGTGATGGCCGCC
TATAACCTGGGGCCTGAGCGCGTGCAGGGCATGCGCGAGGAAGCGCGGCGTCGCGGGCTGAACCCGAACCAGTGGTTCTT
CCAGACCGAGCGTGTGGCGATGGAACAGGGCGGTGCCAATGTCGTGGCGTTCGTGAACAGCGTCAACAAGTACTACCTGG
CCTTCGACCGCGAGCGCGACTCGCTGGAAAAGAGCGGGCCCAAATCTGCGGTAAAACGCTAA

Upstream 100 bases:

>100_bases
AACCTCAAGGTTCAACGATTTCCTTCGCTGTTCGATGCTGCTGCGTGACCTCAAGATTTCCTTTTTCCTGTTTCTTTCCG
GCATTGCCCTGGGGAAGTGA

Downstream 100 bases:

>100_bases
AGATCGATTTATTCGATTTGTATGCGCCGTTATTTGCGGTTTTTACATGAGTGAATTTGATTATCATGGCGCCACTCGCT
GTTAATCACCCATCACTCAC

Product: SLT

Products: NA

Alternate protein names: Murein lyase F [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MIRSALVSLICLLPLLPLQVSARQTGPEVVQHTVQVRDLPAIRSSKVLRVLVNQSRNSSGDVKGQEIGVEYHRLQAFEQY
LNSHSRAGQKVTIKVIPKAKNQLLTALQRGEGDMIAPGELLDVSDAKGIQASAPIVHDVPLVLVGVRGQRSVRRVDQLSG
KTLSLPTGSAADEALHQVNRQLELRKLPLAKIEWVDPSLAVEDVLEMVQAGIYPMTLVEQPIAERWARIMPKLRIERGLT
LQTHGDISWFVRDNATQLRASVDDFLKDYKPSAQQDQVFANAYKNTYKVNNPLVRNNLQRLEQLRPMLQRHADAQGMDWL
DLAALAFKESKLDPSAKGSGGATGLLQITPSAAKSVGVPSIQSADDNIRAGSRYMALIQRKYFSSNRVNERERMAFVMAA
YNLGPERVQGMREEARRRGLNPNQWFFQTERVAMEQGGANVVAFVNSVNKYYLAFDRERDSLEKSGPKSAVKR

Sequences:

>Translated_473_residues
MIRSALVSLICLLPLLPLQVSARQTGPEVVQHTVQVRDLPAIRSSKVLRVLVNQSRNSSGDVKGQEIGVEYHRLQAFEQY
LNSHSRAGQKVTIKVIPKAKNQLLTALQRGEGDMIAPGELLDVSDAKGIQASAPIVHDVPLVLVGVRGQRSVRRVDQLSG
KTLSLPTGSAADEALHQVNRQLELRKLPLAKIEWVDPSLAVEDVLEMVQAGIYPMTLVEQPIAERWARIMPKLRIERGLT
LQTHGDISWFVRDNATQLRASVDDFLKDYKPSAQQDQVFANAYKNTYKVNNPLVRNNLQRLEQLRPMLQRHADAQGMDWL
DLAALAFKESKLDPSAKGSGGATGLLQITPSAAKSVGVPSIQSADDNIRAGSRYMALIQRKYFSSNRVNERERMAFVMAA
YNLGPERVQGMREEARRRGLNPNQWFFQTERVAMEQGGANVVAFVNSVNKYYLAFDRERDSLEKSGPKSAVKR
>Mature_473_residues
MIRSALVSLICLLPLLPLQVSARQTGPEVVQHTVQVRDLPAIRSSKVLRVLVNQSRNSSGDVKGQEIGVEYHRLQAFEQY
LNSHSRAGQKVTIKVIPKAKNQLLTALQRGEGDMIAPGELLDVSDAKGIQASAPIVHDVPLVLVGVRGQRSVRRVDQLSG
KTLSLPTGSAADEALHQVNRQLELRKLPLAKIEWVDPSLAVEDVLEMVQAGIYPMTLVEQPIAERWARIMPKLRIERGLT
LQTHGDISWFVRDNATQLRASVDDFLKDYKPSAQQDQVFANAYKNTYKVNNPLVRNNLQRLEQLRPMLQRHADAQGMDWL
DLAALAFKESKLDPSAKGSGGATGLLQITPSAAKSVGVPSIQSADDNIRAGSRYMALIQRKYFSSNRVNERERMAFVMAA
YNLGPERVQGMREEARRRGLNPNQWFFQTERVAMEQGGANVVAFVNSVNKYYLAFDRERDSLEKSGPKSAVKR

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI171474010, Length=393, Percent_Identity=24.9363867684478, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR001638 [H]

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT [H]

EC number: NA

Molecular weight: Translated: 52714; Mature: 52714

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRSALVSLICLLPLLPLQVSARQTGPEVVQHTVQVRDLPAIRSSKVLRVLVNQSRNSSG
CHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
DVKGQEIGVEYHRLQAFEQYLNSHSRAGQKVTIKVIPKAKNQLLTALQRGEGDMIAPGEL
CCCCHHHCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCEECCCCE
LDVSDAKGIQASAPIVHDVPLVLVGVRGQRSVRRVDQLSGKTLSLPTGSAADEALHQVNR
ECCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHHHH
QLELRKLPLAKIEWVDPSLAVEDVLEMVQAGIYPMTLVEQPIAERWARIMPKLRIERGLT
HHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCE
LQTHGDISWFVRDNATQLRASVDDFLKDYKPSAQQDQVFANAYKNTYKVNNPLVRNNLQR
EEECCCEEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEECCCCHHHHHHHH
LEQLRPMLQRHADAQGMDWLDLAALAFKESKLDPSAKGSGGATGLLQITPSAAKSVGVPS
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHCCCCC
IQSADDNIRAGSRYMALIQRKYFSSNRVNERERMAFVMAAYNLGPERVQGMREEARRRGL
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
NPNQWFFQTERVAMEQGGANVVAFVNSVNKYYLAFDRERDSLEKSGPKSAVKR
CCCHHHHHHHHHHHHCCCCEEEHEECCCCCEEEEECCCHHHHHHCCCHHHHCC
>Mature Secondary Structure
MIRSALVSLICLLPLLPLQVSARQTGPEVVQHTVQVRDLPAIRSSKVLRVLVNQSRNSSG
CHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
DVKGQEIGVEYHRLQAFEQYLNSHSRAGQKVTIKVIPKAKNQLLTALQRGEGDMIAPGEL
CCCCHHHCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCEECCCCE
LDVSDAKGIQASAPIVHDVPLVLVGVRGQRSVRRVDQLSGKTLSLPTGSAADEALHQVNR
ECCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHHHH
QLELRKLPLAKIEWVDPSLAVEDVLEMVQAGIYPMTLVEQPIAERWARIMPKLRIERGLT
HHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCE
LQTHGDISWFVRDNATQLRASVDDFLKDYKPSAQQDQVFANAYKNTYKVNNPLVRNNLQR
EEECCCEEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEECCCCHHHHHHHH
LEQLRPMLQRHADAQGMDWLDLAALAFKESKLDPSAKGSGGATGLLQITPSAAKSVGVPS
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHCCCCC
IQSADDNIRAGSRYMALIQRKYFSSNRVNERERMAFVMAAYNLGPERVQGMREEARRRGL
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
NPNQWFFQTERVAMEQGGANVVAFVNSVNKYYLAFDRERDSLEKSGPKSAVKR
CCCHHHHHHHHHHHHCCCCEEEHEECCCCCEEEEECCCHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA