Click here to switch to the map view.

The map label for this gene is tsf [H]

Identifier: 66044592

GI number: 66044592

Start: 1524151

End: 1525014

Strand: Direct

Name: tsf [H]

Synonym: Psyr_1344

Alternate gene names: 66044592

Gene position: 1524151-1525014 (Clockwise)

Preceding gene: 66044591

Following gene: 66044593

Centisome position: 25.01

GC content: 55.32

Gene sequence:

>864_bases
ATGGCAGAGATTACTGCAGCGTTGGTCAAAGAACTGCGCGAGCGTACCGGCGAAGGCATGATGGATTGCAAAAAGGCCTT
GACCAAGGCTGGCGGCGACATCGAAAAAGCAATCGACGACATGCGTGCTTCCGGCGCCATCAAGGCCGCCAAGAAAGCAG
GCAACGTGGCTGCTGAAGGCGCTATCGCTATCAAGGACGACGGCAAGGCTGCTGTCATCATCGAAGTCAACTCGCAGACC
GACTTCCTGGCCCTGCAGGACGACTTCAAGGCTTTCGTTGCTGCAAGCGTCGAAAAAGCCTTCGCTGACAAGCTGACTGA
TGTCGCTCCGTTGATCGAAGCTCAGGAAGCTGCACGTCTGGTACTGGTCGGCAAGGTTGGCGAAAACGTCAACATCCGTC
GCTTGAAGCGTATCGAAGGTGACGTCGTAGGTACTTACCTGCACGGCAACAAGATCGGCGTGGTAGTTACCCTCAAGGGT
GGCGACGTCGAGCTGGCCAAGGATATCGCCATGCACGTCGCGGCAAGCAACCCCGAGTTCCTGTTCCCTTCGGAAGTTTC
TGCCGAAGCGATCGAGCGCGAGAAGAATGTCTTCCTGCAACTGAACGAAGACAAGATCAAAGGCAAGCCAGCTGAAATCG
TTGAAAAAATGGTTGGCGGTCGTATCACCAAGTTCCTGGCAGAAGCCAGCCTGGTCGAGCAAGCCTTCGTCAAGAACCCG
GAAGTCAAGGTCGGTGATCTGGCCAAGAAAGCCGGCGCTGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGACGGCAT
CGAGAAGCCAGTTGATAACTTCGCTGACGAAGTGGCTGCTCAACTGGCTGCCGCCAAGCAGTAA

Upstream 100 bases:

>100_bases
GACCTCGCTGTCATGTGTTGCTGCCACCCTGATTTTTCAAGAATTGCACGCCCGGCCTGTCGGGTGGAATGGTTGAAGAC
CTATCCAAGAGGATTTTGAA

Downstream 100 bases:

>100_bases
GACGGTTTTTCAACTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGTGCGGCCTCTTTGTCGAAAGGGAAAAGGAAATTTT
CCTTGGAGATTGTGCTCACA

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MAEITAALVKELRERTGEGMMDCKKALTKAGGDIEKAIDDMRASGAIKAAKKAGNVAAEGAIAIKDDGKAAVIIEVNSQT
DFLALQDDFKAFVAASVEKAFADKLTDVAPLIEAQEAARLVLVGKVGENVNIRRLKRIEGDVVGTYLHGNKIGVVVTLKG
GDVELAKDIAMHVAASNPEFLFPSEVSAEAIEREKNVFLQLNEDKIKGKPAEIVEKMVGGRITKFLAEASLVEQAFVKNP
EVKVGDLAKKAGAEIVSFTYFKVGDGIEKPVDNFADEVAAQLAAAKQ

Sequences:

>Translated_287_residues
MAEITAALVKELRERTGEGMMDCKKALTKAGGDIEKAIDDMRASGAIKAAKKAGNVAAEGAIAIKDDGKAAVIIEVNSQT
DFLALQDDFKAFVAASVEKAFADKLTDVAPLIEAQEAARLVLVGKVGENVNIRRLKRIEGDVVGTYLHGNKIGVVVTLKG
GDVELAKDIAMHVAASNPEFLFPSEVSAEAIEREKNVFLQLNEDKIKGKPAEIVEKMVGGRITKFLAEASLVEQAFVKNP
EVKVGDLAKKAGAEIVSFTYFKVGDGIEKPVDNFADEVAAQLAAAKQ
>Mature_286_residues
AEITAALVKELRERTGEGMMDCKKALTKAGGDIEKAIDDMRASGAIKAAKKAGNVAAEGAIAIKDDGKAAVIIEVNSQTD
FLALQDDFKAFVAASVEKAFADKLTDVAPLIEAQEAARLVLVGKVGENVNIRRLKRIEGDVVGTYLHGNKIGVVVTLKGG
DVELAKDIAMHVAASNPEFLFPSEVSAEAIEREKNVFLQLNEDKIKGKPAEIVEKMVGGRITKFLAEASLVEQAFVKNPE
VKVGDLAKKAGAEIVSFTYFKVGDGIEKPVDNFADEVAAQLAAAKQ

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome [H]

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family [H]

Homologues:

Organism=Escherichia coli, GI1786366, Length=280, Percent_Identity=58.9285714285714, Blast_Score=300, Evalue=6e-83,
Organism=Caenorhabditis elegans, GI17561440, Length=295, Percent_Identity=25.7627118644068, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19921466, Length=293, Percent_Identity=26.962457337884, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449 [H]

Pfam domain/function: PF00889 EF_TS; PF00627 UBA [H]

EC number: NA

Molecular weight: Translated: 30523; Mature: 30392

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS01126 EF_TS_1 ; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEITAALVKELRERTGEGMMDCKKALTKAGGDIEKAIDDMRASGAIKAAKKAGNVAAEG
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHCCCEECCC
AIAIKDDGKAAVIIEVNSQTDFLALQDDFKAFVAASVEKAFADKLTDVAPLIEAQEAARL
EEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEE
VLVGKVGENVNIRRLKRIEGDVVGTYLHGNKIGVVVTLKGGDVELAKDIAMHVAASNPEF
EEEECCCCCCCHHHHHHHCCCEEEEEEECCEEEEEEEEECCCCHHHHHHHHHHCCCCCCE
LFPSEVSAEAIEREKNVFLQLNEDKIKGKPAEIVEKMVGGRITKFLAEASLVEQAFVKNP
ECCCCCCHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCC
EVKVGDLAKKAGAEIVSFTYFKVGDGIEKPVDNFADEVAAQLAAAKQ
CCCHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AEITAALVKELRERTGEGMMDCKKALTKAGGDIEKAIDDMRASGAIKAAKKAGNVAAEG
CHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHCCCEECCC
AIAIKDDGKAAVIIEVNSQTDFLALQDDFKAFVAASVEKAFADKLTDVAPLIEAQEAARL
EEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEE
VLVGKVGENVNIRRLKRIEGDVVGTYLHGNKIGVVVTLKGGDVELAKDIAMHVAASNPEF
EEEECCCCCCCHHHHHHHCCCEEEEEEECCEEEEEEEEECCCCHHHHHHHHHHCCCCCCE
LFPSEVSAEAIEREKNVFLQLNEDKIKGKPAEIVEKMVGGRITKFLAEASLVEQAFVKNP
ECCCCCCHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCC
EVKVGDLAKKAGAEIVSFTYFKVGDGIEKPVDNFADEVAAQLAAAKQ
CCCHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]