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The map label for this gene is clpB [H]

Identifier: 66043993

GI number: 66043993

Start: 832326

End: 834890

Strand: Direct

Name: clpB [H]

Synonym: Psyr_0728

Alternate gene names: 66043993

Gene position: 832326-834890 (Clockwise)

Preceding gene: 66043992

Following gene: 66043996

Centisome position: 13.66

GC content: 60.47

Gene sequence:

>2565_bases
ATGCGTATCGACAGATTGACCAGTAAATTACAGTTAGCGTTATCCGATTCACAGTCGCTGGCCGTTGGCCTCGACCATCC
CGCCATCGAACCTGCGCACTTGATGCAGGCGCTGCTTGAGCAGCAGGGCGGTTCCATCAAGCCTCTGCTGTTGCAGGTAG
GCTTCGACATCAACAGCCTGCGCAAGGAACTGAGCGCAGAGCTGGACCGCCTGCCAAAAATCCAGAACCCGACCGGCGAC
GTCAACATGTCGCAGGATCTGGCGCGCCTGCTCAACCAGGCTGACCGTCTGGCGCAGCAGAAAGGCGACCAGTTCATTTC
CAGCGAGCTGGTGCTGCTCGCGGCAATGGACGAGAACAGCAAGCTCGGCAAATTGTTGCTGGGGCAGGGCGTCAGCAAGA
AGGCCCTGGAAAACGCCATCAACAACCTGCGCGGCGAAGGTGCCGTGAATGACCCGAACGTCGAAGAGTCGCGTCAGGCG
CTGGACAAATACACCGTCGACCTGACCAAGCGTGCCGAAGAGGGCAAACTCGACCCGGTGATCGGACGTGATGACGAGAT
TCGTCGCACCATTCAGGTCCTGCAGCGGCGCACCAAGAACAACCCGGTGTTGATCGGCGAACCTGGTGTGGGCAAGACCG
CCATTGCCGAGGGCCTGGCGCAGCGCATCATCAATGGCGAAGTGCCGGATGGCCTGCGCGGCAAGCGCTTGCTGTCGCTG
GACATGGGCGCGTTGATTGCCGGTGCCAAGTACCGTGGCGAGTTCGAAGAGCGCCTCAAATCGCTGCTCAACGAACTGTC
CAAGCAGGAAGGCCAGATCATCCTGTTCATCGACGAGTTGCACACCATGGTCGGCGCCGGCAAGGGCGAAGGCTCGATGG
ACGCTGGCAACATGCTCAAACCTGCGCTGGCGCGCGGCGAGCTGCATTGTGTGGGGGCTACCACGCTCAACGAGTACCGC
CAATATATAGAGAAGGATGCGGCGCTTGAGCGTCGATTCCAGAAAGTCCTGGTCGACGAGCCAAGTGAAGAAGACACCAT
CGCGATCCTGCGTGGCCTCAAAGAGCGCTACGAAGTGCACCACAAGGTTGCCATCACCGACGGCGCGATCATCGCGGCGG
CCAAGCTGAGCCATCGCTATATCACCGACCGCCAGTTGCCGGACAAGGCCATCGACCTGATCGACGAAGCGGCCAGCCGT
ATTCGCATGGAGATCGACTCCAAGCCTGAAGTGCTCGACCGCCTTGAACGTCGCCTGATCCAGCTGAAAGTAGAAGCTCA
GGCGCTCAAGAAGGAAAAGGACGAGGCGGCGATCAAGCGTCTGGAAAAACTCCAGGGTGAAATCGAGCGTCTTGAGCTTG
AGTACGCGGATCTTGAAGAGATCTGGACCTCGGAAAAAGCCGAGGTCACCGGCTCGGCACAGATCCAGCAGAAAATCGAA
CAGTCTCGTCAGGAGCTGGAAGCCGCTCGCCGCCGTGGCGATCTGAACCGCATGGCGGAGTTGCAGTACGGGATCATTCC
CGACCTGGAACGCAGCCTGCAAATGGTCGACCAGCACGGCAAGCCGGAAAACCAGTTGCTGCGCAGCAAGGTGACCGAGG
AAGAAATTGCCGAGGTCGTGTCGAAGTGGACCGGTATCCCGGTGTCGAAGATGCTCGAAGGCGAGCGCGACAAGCTGTTG
CGCATGGAAACCCTGCTGCACAACCGGGTGATCGGCCAGGAAGAAGCGGTGGTCGCGGTCTCCAATGCCGTGCGTCGTTC
GCGTGCCGGGCTTTCCGACCCTAATCGTCCGAGCGGATCGTTCATGTTCCTCGGCCCGACCGGCGTGGGCAAGACCGAGC
TGTGCAAGGCGCTGGCCGAGTTCCTGTTCGACACCGAAGAGGCAATGGTGCGTATCGACATGTCCGAGTTCATGGAGAAA
CATTCCGTGGCTCGCCTGATCGGTGCGCCGCCAGGCTACGTCGGTTACGAAGAGGGCGGCTACCTGACCGAAGCCGTGCG
TCGCAAACCGTATTCGCTGATTCTGCTCGACGAAGTCGAGAAGGCGCATTCCGACGTGTTCAACATCCTCTTGCAGGTGC
TTGAAGACGGACGCTTGACTGACAGCCACGGGCGTACGGTGGACTTCCGCAATACCGTGATCGTCATGACCTCCAACCTG
GGCTCGGCGCAGATCCAGGAACTGGTCGGTGATCGTGAGGCGCAGCGTGCTGCGGTAATGGATGCGGTCGGTACGCACTT
CCGTCCGGAGTTCGTTAACCGGATCGACGAAGTGGTGATCTTCGAACCGCTGGCGCGTGACCAGATTGCCGGTATCACCG
ACATCCAGCTGGGCCGCCTGCGCAAGCGTCTGGCCGAGCGCGAGCTGTCGATGACCCTGAGCCCCGAAGCGCTGGACAAG
CTGATCGCGGTTGGTTACGACCCGGTCTATGGCGCACGGCCGCTGAAGCGGGCGATTCAGCGCTGGATCGAGAACCCGCT
GGCGCAGATGATCCTCTCGGGCAGCTTCATGCCCGGCACGACCATTACCGGCAAGGTGGTCGACGACGAGATCACGTTTG
TCTGA

Upstream 100 bases:

>100_bases
GCTTGAAACGCGCAGAATCGTCCGCATCTAACTGGCTATTCAGCAGGTTTTCTTTATAGGTGTGTTCATTGCTCCGGCCT
GCATTAAAGGAAGGTGACAA

Downstream 100 bases:

>100_bases
CAGCGCTCGTCAACGCTCTATAGAAGGGCCCTCGAACCGAGGGCCTTTTTTTGAGGCGTGGTCAGCAGTAGAATCAGGCC
CGAGAGGCGATCTGAAAAAA

Product: AAA ATPase, central region:Clp, N terminal:Clp, N terminal

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 854; Mature: 854

Protein sequence:

>854_residues
MRIDRLTSKLQLALSDSQSLAVGLDHPAIEPAHLMQALLEQQGGSIKPLLLQVGFDINSLRKELSAELDRLPKIQNPTGD
VNMSQDLARLLNQADRLAQQKGDQFISSELVLLAAMDENSKLGKLLLGQGVSKKALENAINNLRGEGAVNDPNVEESRQA
LDKYTVDLTKRAEEGKLDPVIGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIAEGLAQRIINGEVPDGLRGKRLLSL
DMGALIAGAKYRGEFEERLKSLLNELSKQEGQIILFIDELHTMVGAGKGEGSMDAGNMLKPALARGELHCVGATTLNEYR
QYIEKDAALERRFQKVLVDEPSEEDTIAILRGLKERYEVHHKVAITDGAIIAAAKLSHRYITDRQLPDKAIDLIDEAASR
IRMEIDSKPEVLDRLERRLIQLKVEAQALKKEKDEAAIKRLEKLQGEIERLELEYADLEEIWTSEKAEVTGSAQIQQKIE
QSRQELEAARRRGDLNRMAELQYGIIPDLERSLQMVDQHGKPENQLLRSKVTEEEIAEVVSKWTGIPVSKMLEGERDKLL
RMETLLHNRVIGQEEAVVAVSNAVRRSRAGLSDPNRPSGSFMFLGPTGVGKTELCKALAEFLFDTEEAMVRIDMSEFMEK
HSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSLILLDEVEKAHSDVFNILLQVLEDGRLTDSHGRTVDFRNTVIVMTSNL
GSAQIQELVGDREAQRAAVMDAVGTHFRPEFVNRIDEVVIFEPLARDQIAGITDIQLGRLRKRLAERELSMTLSPEALDK
LIAVGYDPVYGARPLKRAIQRWIENPLAQMILSGSFMPGTTITGKVVDDEITFV

Sequences:

>Translated_854_residues
MRIDRLTSKLQLALSDSQSLAVGLDHPAIEPAHLMQALLEQQGGSIKPLLLQVGFDINSLRKELSAELDRLPKIQNPTGD
VNMSQDLARLLNQADRLAQQKGDQFISSELVLLAAMDENSKLGKLLLGQGVSKKALENAINNLRGEGAVNDPNVEESRQA
LDKYTVDLTKRAEEGKLDPVIGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIAEGLAQRIINGEVPDGLRGKRLLSL
DMGALIAGAKYRGEFEERLKSLLNELSKQEGQIILFIDELHTMVGAGKGEGSMDAGNMLKPALARGELHCVGATTLNEYR
QYIEKDAALERRFQKVLVDEPSEEDTIAILRGLKERYEVHHKVAITDGAIIAAAKLSHRYITDRQLPDKAIDLIDEAASR
IRMEIDSKPEVLDRLERRLIQLKVEAQALKKEKDEAAIKRLEKLQGEIERLELEYADLEEIWTSEKAEVTGSAQIQQKIE
QSRQELEAARRRGDLNRMAELQYGIIPDLERSLQMVDQHGKPENQLLRSKVTEEEIAEVVSKWTGIPVSKMLEGERDKLL
RMETLLHNRVIGQEEAVVAVSNAVRRSRAGLSDPNRPSGSFMFLGPTGVGKTELCKALAEFLFDTEEAMVRIDMSEFMEK
HSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSLILLDEVEKAHSDVFNILLQVLEDGRLTDSHGRTVDFRNTVIVMTSNL
GSAQIQELVGDREAQRAAVMDAVGTHFRPEFVNRIDEVVIFEPLARDQIAGITDIQLGRLRKRLAERELSMTLSPEALDK
LIAVGYDPVYGARPLKRAIQRWIENPLAQMILSGSFMPGTTITGKVVDDEITFV
>Mature_854_residues
MRIDRLTSKLQLALSDSQSLAVGLDHPAIEPAHLMQALLEQQGGSIKPLLLQVGFDINSLRKELSAELDRLPKIQNPTGD
VNMSQDLARLLNQADRLAQQKGDQFISSELVLLAAMDENSKLGKLLLGQGVSKKALENAINNLRGEGAVNDPNVEESRQA
LDKYTVDLTKRAEEGKLDPVIGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIAEGLAQRIINGEVPDGLRGKRLLSL
DMGALIAGAKYRGEFEERLKSLLNELSKQEGQIILFIDELHTMVGAGKGEGSMDAGNMLKPALARGELHCVGATTLNEYR
QYIEKDAALERRFQKVLVDEPSEEDTIAILRGLKERYEVHHKVAITDGAIIAAAKLSHRYITDRQLPDKAIDLIDEAASR
IRMEIDSKPEVLDRLERRLIQLKVEAQALKKEKDEAAIKRLEKLQGEIERLELEYADLEEIWTSEKAEVTGSAQIQQKIE
QSRQELEAARRRGDLNRMAELQYGIIPDLERSLQMVDQHGKPENQLLRSKVTEEEIAEVVSKWTGIPVSKMLEGERDKLL
RMETLLHNRVIGQEEAVVAVSNAVRRSRAGLSDPNRPSGSFMFLGPTGVGKTELCKALAEFLFDTEEAMVRIDMSEFMEK
HSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSLILLDEVEKAHSDVFNILLQVLEDGRLTDSHGRTVDFRNTVIVMTSNL
GSAQIQELVGDREAQRAAVMDAVGTHFRPEFVNRIDEVVIFEPLARDQIAGITDIQLGRLRKRLAERELSMTLSPEALDK
LIAVGYDPVYGARPLKRAIQRWIENPLAQMILSGSFMPGTTITGKVVDDEITFV

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=320, Percent_Identity=35.625, Blast_Score=201, Evalue=2e-51,
Organism=Escherichia coli, GI1788943, Length=856, Percent_Identity=69.0420560747664, Blast_Score=1190, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=414, Percent_Identity=41.304347826087, Blast_Score=283, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6320464, Length=711, Percent_Identity=52.8832630098453, Blast_Score=752, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=857, Percent_Identity=42.8238039673279, Blast_Score=663, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150
- InterPro:   IPR002078 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 95199; Mature: 95199

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDRLTSKLQLALSDSQSLAVGLDHPAIEPAHLMQALLEQQGGSIKPLLLQVGFDINSL
CCHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHEECCCHHHH
RKELSAELDRLPKIQNPTGDVNMSQDLARLLNQADRLAQQKGDQFISSELVLLAAMDENS
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCC
KLGKLLLGQGVSKKALENAINNLRGEGAVNDPNVEESRQALDKYTVDLTKRAEEGKLDPV
CHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
IGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIAEGLAQRIINGEVPDGLRGKRLLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DMGALIAGAKYRGEFEERLKSLLNELSKQEGQIILFIDELHTMVGAGKGEGSMDAGNMLK
CHHHHHHCCHHCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCCHHH
PALARGELHCVGATTLNEYRQYIEKDAALERRFQKVLVDEPSEEDTIAILRGLKERYEVH
HHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
HKVAITDGAIIAAAKLSHRYITDRQLPDKAIDLIDEAASRIRMEIDSKPEVLDRLERRLI
HHEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QLKVEAQALKKEKDEAAIKRLEKLQGEIERLELEYADLEEIWTSEKAEVTGSAQIQQKIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
QSRQELEAARRRGDLNRMAELQYGIIPDLERSLQMVDQHGKPENQLLRSKVTEEEIAEVV
HHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHH
SKWTGIPVSKMLEGERDKLLRMETLLHNRVIGQEEAVVAVSNAVRRSRAGLSDPNRPSGS
HHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
FMFLGPTGVGKTELCKALAEFLFDTEEAMVRIDMSEFMEKHSVARLIGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHHCCHHHHEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAVRRKPYSLILLDEVEKAHSDVFNILLQVLEDGRLTDSHGRTVDFRNTVIVMTSNL
HHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCEEEEEECCC
GSAQIQELVGDREAQRAAVMDAVGTHFRPEFVNRIDEVVIFEPLARDQIAGITDIQLGRL
CHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHEEEECCHHHHHHCCCCHHHHHHH
RKRLAERELSMTLSPEALDKLIAVGYDPVYGARPLKRAIQRWIENPLAQMILSGSFMPGT
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TITGKVVDDEITFV
CEECEEECCCCCCC
>Mature Secondary Structure
MRIDRLTSKLQLALSDSQSLAVGLDHPAIEPAHLMQALLEQQGGSIKPLLLQVGFDINSL
CCHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHEECCCHHHH
RKELSAELDRLPKIQNPTGDVNMSQDLARLLNQADRLAQQKGDQFISSELVLLAAMDENS
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCC
KLGKLLLGQGVSKKALENAINNLRGEGAVNDPNVEESRQALDKYTVDLTKRAEEGKLDPV
CHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
IGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIAEGLAQRIINGEVPDGLRGKRLLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DMGALIAGAKYRGEFEERLKSLLNELSKQEGQIILFIDELHTMVGAGKGEGSMDAGNMLK
CHHHHHHCCHHCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCCHHH
PALARGELHCVGATTLNEYRQYIEKDAALERRFQKVLVDEPSEEDTIAILRGLKERYEVH
HHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
HKVAITDGAIIAAAKLSHRYITDRQLPDKAIDLIDEAASRIRMEIDSKPEVLDRLERRLI
HHEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QLKVEAQALKKEKDEAAIKRLEKLQGEIERLELEYADLEEIWTSEKAEVTGSAQIQQKIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
QSRQELEAARRRGDLNRMAELQYGIIPDLERSLQMVDQHGKPENQLLRSKVTEEEIAEVV
HHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHH
SKWTGIPVSKMLEGERDKLLRMETLLHNRVIGQEEAVVAVSNAVRRSRAGLSDPNRPSGS
HHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
FMFLGPTGVGKTELCKALAEFLFDTEEAMVRIDMSEFMEKHSVARLIGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHHCCHHHHEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAVRRKPYSLILLDEVEKAHSDVFNILLQVLEDGRLTDSHGRTVDFRNTVIVMTSNL
HHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCEEEEEECCC
GSAQIQELVGDREAQRAAVMDAVGTHFRPEFVNRIDEVVIFEPLARDQIAGITDIQLGRL
CHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHEEEECCHHHHHHCCCCHHHHHHH
RKRLAERELSMTLSPEALDKLIAVGYDPVYGARPLKRAIQRWIENPLAQMILSGSFMPGT
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TITGKVVDDEITFV
CEECEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]