Definition Pseudomonas syringae pv. syringae B728a, complete genome.
Accession NC_007005
Length 6,093,698

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The map label for this gene is recD [H]

Identifier: 66043949

GI number: 66043949

Start: 784699

End: 786792

Strand: Direct

Name: recD [H]

Synonym: Psyr_0682

Alternate gene names: 66043949

Gene position: 784699-786792 (Clockwise)

Preceding gene: 66043948

Following gene: 66043950

Centisome position: 12.88

GC content: 64.33

Gene sequence:

>2094_bases
ATGAGCCGCTCGTTCGATGATCTGTTGCCTACCGCGCTGGACGACATCAGCCTCGCTGAGTTGAGCCCGCTGACGCGTGT
CAGCGATCTGCTGATCCTGCTCGAACGTTGGGTCGAGCGCGGCTGGTTGCGTGCTCTGGACAAGGCGTTCGTCGCGTTCC
TCAGTGACCTCGATCCGCAGGCCGATCCGTTGGTGCTGGTGGCTGCGGCGCTTACCAGCCATCAGCTCGGCCACGGTCAC
GTCTGCCTTGATCTGTACGAGACCCTCAAAGAACCGGACTTCGCCCTGTCACTGCCACCTGAAGGCGATCAGCAGAGCGC
GCCGATGCTGCTCCCCTCACAACTGTTGGCAGCGTTGGATGGTGCCGCTTGGTGCCAGGCGCTGGCAGCCAGCATGCTGG
TCGCCGAGGTGGGCGATTTAAGTGCCGAAGCGCGACAAAAGCCATTGGTGCTGGCCGAGCGGCGTCTGTACCTGCGTCGC
TACTGGACTTACGAACGGCGTATTGCTGCCGCCTTGCGTCAGCGCCTGGCCCAGCGTGAAACACCGCCGGAGGGTCTGCC
GCAGCGGCTCGACGCATTGTTCGGCCCGGCCGATTCTTCGCCACAAGCCGTCATCGACTGGCAGAAACTTGCCTGCGCAC
TGGCGACCCGCAAGGGCTTCAGCATCATCACCGGCGGCCCCGGCACCGGCAAAACCACCACGGTGGTGCGCCTGCTGGCT
TTGTTGCAGGCACCGGCGGTACAGAGTGGCCAGCCGCTGCGCATCCGTCTCGCGGCACCCACCGGCAAGGCGGCGGCACG
TCTGACCGAGTCCATCAGCCAACAGGTGCAGAGCCTGGACGTCAGTGACGACGTACGCCAGAAAATCCCCAGCGAGGTGA
CCACCGTCCACCGCCTGCTGGGCAGTCGCCCCGGTACCCGGCACTTCCGCCACCATGCGGGTAATTTACTGCCGCTGGAT
GTGCTGGTGGTTGATGAAGCCTCCATGATCGACCTGGAAATGATGGCTAACCTGCTCTACGCACTGCCTGCGCATGCACG
CATGGTTCTGCTGGGTGACAAGGACCAGCTGGCATCCGTTGAGGCGGGGGCGGTACTCGGGGATTTGTGCCGCGACGCCG
AAGAAGGTTTCTACAGCCCCGACACCCAGGCCTGGCTGGAAGCCGTCAGCGGCGAAGACCTCGGCAAGGGCGGTTTGCGC
CAGGGCGATGCGCAGCAGCATCCGTTGGCCCAGCAAGTGGTGATGCTGCGCCACTCACGTCGTTTCGGCCAAGGCAGCGG
TATCGGCCAGTTGGCCCGGCTGGTCAACCAGCAACAGGACCAACAGGCCCGCGCGCTGCTGTCGGCGGGCAGTCATGACG
ACCTGTTCGCCTTGGCTCTCAAGGGCGAGCAGGACCTCAAGTTCGAACGCCTGGTGCTCGACGGTCTTGGCAGGGGCGAG
CAAGGTCCGCAGGGTTACCGGCATTACCTGAACGTACTCGCCGCCGAGCGTCCCGCCGATGATTCGGCACTGGACGACGC
GTGCTGGACCCGCTGGGCGCGCTCGGTACTCAGCGCCTTCGATGCCTTTCAACTGCTGTGTGCCGTGCGCAAAGGCCCGT
GGGGCGTGGAAGGCTTGAACCAGCGCATCACGCACGCGCTGTTCAGCGCGAAGCTGATCGAAAGCGAACAGCAATGGTAC
GAAGGTCGCCCGGTGCTGATGACCCACAACGACTACGGCCTCGGCCTGATGAACGGCGACATCGGCATCACTTTGCGCCT
GCCCGAGCGCGACGGCGAGGGCAAACAGGTGTTGCGCGTCGCCTTCCCCCGCAACGACGGCAGCGAAGGCGTACGCTTCG
TCCTGCCCAGCCGCCTCAACGAGGTCGAAACCGTGTACGCCATGACCGTACACAAATCCCAGGGCTCGGAATTCGCCCAC
ACCGCCCTGATCCTGCCCGAAGCGCTCAACCCGGTACTGACCAAGGAGCTCATCTACACCGGCATCACCCGCGCCAAGCA
CTGGTTCAGCCTCATCGAACCGCGCCAGGGCATCTTCGAAGAAGCCCTGCGCCGCAAGGTCAAACGCCTGAGTGGGTTGA
TGCTGGGGCTTTGA

Upstream 100 bases:

>100_bases
AGGGCGCGTATTTCGCCCGTCCGCCACGTGAGCTGATCGAGAGTCTTGACCTGCTGTTTCAGGGCAAACCGATAGCGCAT
GACCTGCCGGGAGCATTGGC

Downstream 100 bases:

>100_bases
TCATGTCCGTGCTGTCTGGCGATTCGTTACCGTGCATTGCAGGTACGAAGTCGAGCGCCATAATCCCTATGCGCTTCAAC
CGGGTTCAAGGGTCAATTGA

Product: exodeoxyribonuclease V, alpha subunit

Products: NA

Alternate protein names: Exodeoxyribonuclease V 67 kDa polypeptide [H]

Number of amino acids: Translated: 697; Mature: 696

Protein sequence:

>697_residues
MSRSFDDLLPTALDDISLAELSPLTRVSDLLILLERWVERGWLRALDKAFVAFLSDLDPQADPLVLVAAALTSHQLGHGH
VCLDLYETLKEPDFALSLPPEGDQQSAPMLLPSQLLAALDGAAWCQALAASMLVAEVGDLSAEARQKPLVLAERRLYLRR
YWTYERRIAAALRQRLAQRETPPEGLPQRLDALFGPADSSPQAVIDWQKLACALATRKGFSIITGGPGTGKTTTVVRLLA
LLQAPAVQSGQPLRIRLAAPTGKAAARLTESISQQVQSLDVSDDVRQKIPSEVTTVHRLLGSRPGTRHFRHHAGNLLPLD
VLVVDEASMIDLEMMANLLYALPAHARMVLLGDKDQLASVEAGAVLGDLCRDAEEGFYSPDTQAWLEAVSGEDLGKGGLR
QGDAQQHPLAQQVVMLRHSRRFGQGSGIGQLARLVNQQQDQQARALLSAGSHDDLFALALKGEQDLKFERLVLDGLGRGE
QGPQGYRHYLNVLAAERPADDSALDDACWTRWARSVLSAFDAFQLLCAVRKGPWGVEGLNQRITHALFSAKLIESEQQWY
EGRPVLMTHNDYGLGLMNGDIGITLRLPERDGEGKQVLRVAFPRNDGSEGVRFVLPSRLNEVETVYAMTVHKSQGSEFAH
TALILPEALNPVLTKELIYTGITRAKHWFSLIEPRQGIFEEALRRKVKRLSGLMLGL

Sequences:

>Translated_697_residues
MSRSFDDLLPTALDDISLAELSPLTRVSDLLILLERWVERGWLRALDKAFVAFLSDLDPQADPLVLVAAALTSHQLGHGH
VCLDLYETLKEPDFALSLPPEGDQQSAPMLLPSQLLAALDGAAWCQALAASMLVAEVGDLSAEARQKPLVLAERRLYLRR
YWTYERRIAAALRQRLAQRETPPEGLPQRLDALFGPADSSPQAVIDWQKLACALATRKGFSIITGGPGTGKTTTVVRLLA
LLQAPAVQSGQPLRIRLAAPTGKAAARLTESISQQVQSLDVSDDVRQKIPSEVTTVHRLLGSRPGTRHFRHHAGNLLPLD
VLVVDEASMIDLEMMANLLYALPAHARMVLLGDKDQLASVEAGAVLGDLCRDAEEGFYSPDTQAWLEAVSGEDLGKGGLR
QGDAQQHPLAQQVVMLRHSRRFGQGSGIGQLARLVNQQQDQQARALLSAGSHDDLFALALKGEQDLKFERLVLDGLGRGE
QGPQGYRHYLNVLAAERPADDSALDDACWTRWARSVLSAFDAFQLLCAVRKGPWGVEGLNQRITHALFSAKLIESEQQWY
EGRPVLMTHNDYGLGLMNGDIGITLRLPERDGEGKQVLRVAFPRNDGSEGVRFVLPSRLNEVETVYAMTVHKSQGSEFAH
TALILPEALNPVLTKELIYTGITRAKHWFSLIEPRQGIFEEALRRKVKRLSGLMLGL
>Mature_696_residues
SRSFDDLLPTALDDISLAELSPLTRVSDLLILLERWVERGWLRALDKAFVAFLSDLDPQADPLVLVAAALTSHQLGHGHV
CLDLYETLKEPDFALSLPPEGDQQSAPMLLPSQLLAALDGAAWCQALAASMLVAEVGDLSAEARQKPLVLAERRLYLRRY
WTYERRIAAALRQRLAQRETPPEGLPQRLDALFGPADSSPQAVIDWQKLACALATRKGFSIITGGPGTGKTTTVVRLLAL
LQAPAVQSGQPLRIRLAAPTGKAAARLTESISQQVQSLDVSDDVRQKIPSEVTTVHRLLGSRPGTRHFRHHAGNLLPLDV
LVVDEASMIDLEMMANLLYALPAHARMVLLGDKDQLASVEAGAVLGDLCRDAEEGFYSPDTQAWLEAVSGEDLGKGGLRQ
GDAQQHPLAQQVVMLRHSRRFGQGSGIGQLARLVNQQQDQQARALLSAGSHDDLFALALKGEQDLKFERLVLDGLGRGEQ
GPQGYRHYLNVLAAERPADDSALDDACWTRWARSVLSAFDAFQLLCAVRKGPWGVEGLNQRITHALFSAKLIESEQQWYE
GRPVLMTHNDYGLGLMNGDIGITLRLPERDGEGKQVLRVAFPRNDGSEGVRFVLPSRLNEVETVYAMTVHKSQGSEFAHT
ALILPEALNPVLTKELIYTGITRAKHWFSLIEPRQGIFEEALRRKVKRLSGLMLGL

Specific function: Exhibits several catalytic activities, including ATP- dependent exonuclease, ATP-stimulated endonuclease, ATP-dependent unwinding and DNA-dependent ATPase activities. Strand cleavage occurs 5' to 3' during the unwinding of duplex DNA at CHI sequences, whi

COG id: COG0507

COG function: function code L; ATP-dependent exoDNAse (exonuclease V), alpha subunit - helicase superfamily I member

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recD family [H]

Homologues:

Organism=Escherichia coli, GI1789182, Length=658, Percent_Identity=42.4012158054711, Blast_Score=395, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007807
- InterPro:   IPR006344
- InterPro:   IPR000606 [H]

Pfam domain/function: PF05127 DUF699; PF01443 Viral_helicase1 [H]

EC number: =3.1.11.5 [H]

Molecular weight: Translated: 76566; Mature: 76434

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSFDDLLPTALDDISLAELSPLTRVSDLLILLERWVERGWLRALDKAFVAFLSDLDPQ
CCCCHHHHHHHHHCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ADPLVLVAAALTSHQLGHGHVCLDLYETLKEPDFALSLPPEGDQQSAPMLLPSQLLAALD
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCHHHHHHHHC
GAAWCQALAASMLVAEVGDLSAEARQKPLVLAERRLYLRRYWTYERRIAAALRQRLAQRE
HHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TPPEGLPQRLDALFGPADSSPQAVIDWQKLACALATRKGFSIITGGPGTGKTTTVVRLLA
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHH
LLQAPAVQSGQPLRIRLAAPTGKAAARLTESISQQVQSLDVSDDVRQKIPSEVTTVHRLL
HHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHH
GSRPGTRHFRHHAGNLLPLDVLVVDEASMIDLEMMANLLYALPAHARMVLLGDKDQLASV
CCCCCCHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHH
EAGAVLGDLCRDAEEGFYSPDTQAWLEAVSGEDLGKGGLRQGDAQQHPLAQQVVMLRHSR
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
RFGQGSGIGQLARLVNQQQDQQARALLSAGSHDDLFALALKGEQDLKFERLVLDGLGRGE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCC
QGPQGYRHYLNVLAAERPADDSALDDACWTRWARSVLSAFDAFQLLCAVRKGPWGVEGLN
CCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
QRITHALFSAKLIESEQQWYEGRPVLMTHNDYGLGLMNGDIGITLRLPERDGEGKQVLRV
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEECCCEEEEEEECCCCCCCCEEEEE
AFPRNDGSEGVRFVLPSRLNEVETVYAMTVHKSQGSEFAHTALILPEALNPVLTKELIYT
EECCCCCCCCCEEECCHHCCHHHHHHEEEEECCCCCCHHHHHHHCHHHHCHHHHHHHHHH
GITRAKHWFSLIEPRQGIFEEALRRKVKRLSGLMLGL
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRSFDDLLPTALDDISLAELSPLTRVSDLLILLERWVERGWLRALDKAFVAFLSDLDPQ
CCCHHHHHHHHHCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ADPLVLVAAALTSHQLGHGHVCLDLYETLKEPDFALSLPPEGDQQSAPMLLPSQLLAALD
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCHHHHHHHHC
GAAWCQALAASMLVAEVGDLSAEARQKPLVLAERRLYLRRYWTYERRIAAALRQRLAQRE
HHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TPPEGLPQRLDALFGPADSSPQAVIDWQKLACALATRKGFSIITGGPGTGKTTTVVRLLA
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHH
LLQAPAVQSGQPLRIRLAAPTGKAAARLTESISQQVQSLDVSDDVRQKIPSEVTTVHRLL
HHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHH
GSRPGTRHFRHHAGNLLPLDVLVVDEASMIDLEMMANLLYALPAHARMVLLGDKDQLASV
CCCCCCHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHH
EAGAVLGDLCRDAEEGFYSPDTQAWLEAVSGEDLGKGGLRQGDAQQHPLAQQVVMLRHSR
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
RFGQGSGIGQLARLVNQQQDQQARALLSAGSHDDLFALALKGEQDLKFERLVLDGLGRGE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCC
QGPQGYRHYLNVLAAERPADDSALDDACWTRWARSVLSAFDAFQLLCAVRKGPWGVEGLN
CCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
QRITHALFSAKLIESEQQWYEGRPVLMTHNDYGLGLMNGDIGITLRLPERDGEGKQVLRV
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEECCCEEEEEEECCCCCCCCEEEEE
AFPRNDGSEGVRFVLPSRLNEVETVYAMTVHKSQGSEFAHTALILPEALNPVLTKELIYT
EECCCCCCCCCEEECCHHCCHHHHHHEEEEECCCCCCHHHHHHHCHHHHCHHHHHHHHHH
GITRAKHWFSLIEPRQGIFEEALRRKVKRLSGLMLGL
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3537961; 9278503; 3537960 [H]