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The map label for this gene is ydgK [H]

Identifier: 66043924

GI number: 66043924

Start: 744766

End: 745962

Strand: Direct

Name: ydgK [H]

Synonym: Psyr_0657

Alternate gene names: 66043924

Gene position: 744766-745962 (Clockwise)

Preceding gene: 66043923

Following gene: 66043926

Centisome position: 12.22

GC content: 63.83

Gene sequence:

>1197_bases
ATGAATCTACGAACGATCCTTATCCTTGGCTCATTGAGCGCCTTCGGCCCTCTGGCCATCGACTTCTATCTTCCTGGCTT
TCCGGCCATGGCCTCGTATTTCGGGACCGATGAAAAACACGTACAACTGACCCTGGCGGCCTACTTTCTGGGACTCTCCC
TGGGGCAACTGGCCTATGGCCCGGTGGCTGACCGGTTCGGTCGGCGCATTCCGCTTCTGGTCGGCGTAACCCTGTTCATG
CTTGCCTCGGTCGCCTGCGCCTTTGCGCCGAGTCTTGAATGGCTGATTGCCGCGCGCTTCGTGCAGGCGTTGGGCGGCTG
TGCCGGGATGGTCTTGTCACGCGCCATCGTCAGCGACAAGTGCAATGCCGTCGAGTCGGCCAAGGTTTTCTCGCAACTGA
TGCTGGTCATGGGCCTGGCACCGATTCTGGCCCCGATGCTGGGTGGTGTGCTGGTCAGTACCTTTGGCTGGCAATCGATC
TTCGTCAGCCTCGCCCTGTTCAGCGGTGCCTGCCTGGCGGCGGTTGCGCTGGGGTTGCCGGAAAGCATGCCGGCCACCAC
ACCGCGTCAGCCATTGAGCGGCGCGCTGGGCCAGTACCTCAATCTGTTCAAGGACGGTGTGTTCATCGGTCACGCGCTGA
CCGGCGGCATCGCCATGGCCGGGATGTTCGCCTACGTCGCAGGCTCGCCGTTTGTGTTCATCAAACTGTATGGCGTGCCT
GCCGAGCACTACGGCTGGCTGTTCGGGATGAATGCGGCGGGCTTCATTCTGGTGGCGCAGATCAACGCGCGTATCTTGCG
CAAGACCGGCCCGGCGTTTTTGCTGTCGCGCACGGTCTGGATTTATCTGGCTGCCGGGCTGGTGTTGCTGGCGATCAGCG
GGCTGCGCACCGAGGCGCTTTGGCCACTGCTGGTGCCGCTGTTCATCTGCGTGGCAAGCCTGGGCTGCATCATCCCCAAT
GCGTCGGCCTGCGCCATGAATGGCCAATGGGCGCGCGCGGGCAGTGCCTCGGCGTTGCTGGGTTGTCTGCAATTCAGCGT
TGCCGCCGTCGCTGCCAGTCTGGTGGGCGTGCTGCATGACGGCACGGCCACACCGATGGCGCTGGTCATCAGCCTGTGCG
GCGTGCTGACCGTGGGCCTCTCGGTGCTGACGCGACGTGCGCAGGCCCGGCGCGACGCATCGCTCCAGACCGCCTGA

Upstream 100 bases:

>100_bases
AAACATTGAAAAGTTTTGAATTAATTCCCCCGTTCGTCGTTCTCTGCAAGGTAAGCCGGGTAAACTAGCCCGTTGCTCTG
CATCTGTGAATGGACACCCG

Downstream 100 bases:

>100_bases
TCGCCAAAGCGGTGGTGTCAGTCGCGCCGGGCGTGACTGACACCCTCGACTTCAGACGGCTCAGAAACCTTCCAGTACGA
CCTTGCCCTTGGCCTTGCCG

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MNLRTILILGSLSAFGPLAIDFYLPGFPAMASYFGTDEKHVQLTLAAYFLGLSLGQLAYGPVADRFGRRIPLLVGVTLFM
LASVACAFAPSLEWLIAARFVQALGGCAGMVLSRAIVSDKCNAVESAKVFSQLMLVMGLAPILAPMLGGVLVSTFGWQSI
FVSLALFSGACLAAVALGLPESMPATTPRQPLSGALGQYLNLFKDGVFIGHALTGGIAMAGMFAYVAGSPFVFIKLYGVP
AEHYGWLFGMNAAGFILVAQINARILRKTGPAFLLSRTVWIYLAAGLVLLAISGLRTEALWPLLVPLFICVASLGCIIPN
ASACAMNGQWARAGSASALLGCLQFSVAAVAASLVGVLHDGTATPMALVISLCGVLTVGLSVLTRRAQARRDASLQTA

Sequences:

>Translated_398_residues
MNLRTILILGSLSAFGPLAIDFYLPGFPAMASYFGTDEKHVQLTLAAYFLGLSLGQLAYGPVADRFGRRIPLLVGVTLFM
LASVACAFAPSLEWLIAARFVQALGGCAGMVLSRAIVSDKCNAVESAKVFSQLMLVMGLAPILAPMLGGVLVSTFGWQSI
FVSLALFSGACLAAVALGLPESMPATTPRQPLSGALGQYLNLFKDGVFIGHALTGGIAMAGMFAYVAGSPFVFIKLYGVP
AEHYGWLFGMNAAGFILVAQINARILRKTGPAFLLSRTVWIYLAAGLVLLAISGLRTEALWPLLVPLFICVASLGCIIPN
ASACAMNGQWARAGSASALLGCLQFSVAAVAASLVGVLHDGTATPMALVISLCGVLTVGLSVLTRRAQARRDASLQTA
>Mature_398_residues
MNLRTILILGSLSAFGPLAIDFYLPGFPAMASYFGTDEKHVQLTLAAYFLGLSLGQLAYGPVADRFGRRIPLLVGVTLFM
LASVACAFAPSLEWLIAARFVQALGGCAGMVLSRAIVSDKCNAVESAKVFSQLMLVMGLAPILAPMLGGVLVSTFGWQSI
FVSLALFSGACLAAVALGLPESMPATTPRQPLSGALGQYLNLFKDGVFIGHALTGGIAMAGMFAYVAGSPFVFIKLYGVP
AEHYGWLFGMNAAGFILVAQINARILRKTGPAFLLSRTVWIYLAAGLVLLAISGLRTEALWPLLVPLFICVASLGCIIPN
ASACAMNGQWARAGSASALLGCLQFSVAAVAASLVGVLHDGTATPMALVISLCGVLTVGLSVLTRRAQARRDASLQTA

Specific function: Involved In Sulfonamide (Sulfathiazole) And Bicyclomycin Resistance. Probable Membrane Translocase. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=389, Percent_Identity=32.3907455012853, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI48994889, Length=397, Percent_Identity=30.4785894206549, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI87082312, Length=343, Percent_Identity=30.3206997084548, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1790146, Length=278, Percent_Identity=28.4172661870504, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787065, Length=253, Percent_Identity=30.0395256916996, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1790794, Length=394, Percent_Identity=25.1269035532995, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1790195, Length=136, Percent_Identity=33.0882352941176, Blast_Score=63, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324264, Length=160, Percent_Identity=28.75, Blast_Score=70, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41497; Mature: 41497

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLRTILILGSLSAFGPLAIDFYLPGFPAMASYFGTDEKHVQLTLAAYFLGLSLGQLAYG
CCCEEEEEEECCCCCCCEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
PVADRFGRRIPLLVGVTLFMLASVACAFAPSLEWLIAARFVQALGGCAGMVLSRAIVSDK
CHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CNAVESAKVFSQLMLVMGLAPILAPMLGGVLVSTFGWQSIFVSLALFSGACLAAVALGLP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ESMPATTPRQPLSGALGQYLNLFKDGVFIGHALTGGIAMAGMFAYVAGSPFVFIKLYGVP
CCCCCCCCCCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
AEHYGWLFGMNAAGFILVAQINARILRKTGPAFLLSRTVWIYLAAGLVLLAISGLRTEAL
HHHCCEEEECCCCCEEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
WPLLVPLFICVASLGCIIPNASACAMNGQWARAGSASALLGCLQFSVAAVAASLVGVLHD
HHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
GTATPMALVISLCGVLTVGLSVLTRRAQARRDASLQTA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNLRTILILGSLSAFGPLAIDFYLPGFPAMASYFGTDEKHVQLTLAAYFLGLSLGQLAYG
CCCEEEEEEECCCCCCCEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
PVADRFGRRIPLLVGVTLFMLASVACAFAPSLEWLIAARFVQALGGCAGMVLSRAIVSDK
CHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CNAVESAKVFSQLMLVMGLAPILAPMLGGVLVSTFGWQSIFVSLALFSGACLAAVALGLP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ESMPATTPRQPLSGALGQYLNLFKDGVFIGHALTGGIAMAGMFAYVAGSPFVFIKLYGVP
CCCCCCCCCCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
AEHYGWLFGMNAAGFILVAQINARILRKTGPAFLLSRTVWIYLAAGLVLLAISGLRTEAL
HHHCCEEEECCCCCEEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
WPLLVPLFICVASLGCIIPNASACAMNGQWARAGSASALLGCLQFSVAAVAASLVGVLHD
HHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
GTATPMALVISLCGVLTVGLSVLTRRAQARRDASLQTA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 9202461 [H]