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The map label for this gene is dnaB [H]

Identifier: 66043852

GI number: 66043852

Start: 665791

End: 667185

Strand: Direct

Name: dnaB [H]

Synonym: Psyr_0585

Alternate gene names: 66043852

Gene position: 665791-667185 (Clockwise)

Preceding gene: 66043851

Following gene: 66043854

Centisome position: 10.93

GC content: 60.0

Gene sequence:

>1395_bases
ATGAACGATATTTCCGCCCCCGAGCAATACGATCTGCAAACCGCCGCCCTGAAGGTGCCGCCACATTCCATTGAGGCCGA
ACAGGCTGTACTCGGTGGTCTGATGCTGGACAACAACGCCTGGGAGCGGGTGCTCGATCAAGTTTCCGATGGCGATTTCT
ATCGACATGACCACCGCCTGATATTCCGCGCCATCGCCCGATTGGCCGACCAGAACAGCCCGATCGACGTGGTCACCCTG
GCTGAACAGCTGGACAAGGAAGGGCAGACCTCGCAAGTGGGCGGCCTGGGCTACCTCAGTGAGCTGGCGAAAAACACGCC
TTCTGTCGCCAACATCAAGGCCTACGCGCAGATCGTCCGGGCCCGGGCCACGCTTCGGCAGTTGATCGGCATCAGTACCG
AAATCGCCGACAGTGCGTTCAACCCCGAAGGCCGGACCGCCGAAGAGATTCTTGACGAGGCTGAACGGCAGATCTTCCAG
ATCGCCGAAGCGCGCCCCAAGACCGGCGGTCCGGTCAGCGTCAACGACCTGCTGACCAAGGCCATCGACCGTATCGATAC
GCTGTTCAACAGCGACAACGCCATTACCGGGCTGTCGACCGGGTATACCGATCTCGATGAAAAGACCAGCGGACTGCAAC
CCTCCGACCTGATCATCGTCGCCGGACGTCCGTCGATGGGTAAGACCACCTTCGCCATGAACCTGGTCGAGAACGCGGTG
CTGCGCAGTGACAAGGTGGTGCTGGTCTACTCGCTGGAGATGCCAGGCGAATCGCTGATCATGCGTATGTTGTCCTCGCT
GGGGCGCATCGACCAGACCAAGGTGCGGGCCGGCCAGCTTGATGACGATGACTGGCCGCGTCTGACATCGGCGGTCAATC
TGCTCAATGACCGCAAGCTGTTCATCGACGACACGGCCGGTATCAGCCCGTCCGAAATGCGCGCGCGGACGCGGCGGATC
GTGCGTGAGCATGGCGACATCGCCATGATCATGATCGACTACCTGCAGTTGATGCAGATTCCCGGCTCCAGTGGCGACAA
CCGTACCAACGAGATTTCCGAGATCTCGCGCTCGTTGAAAGCACTGGCCAAGGAATTCAACTGCCCGGTGATTGCCCTGT
CGCAGCTCAACCGCTCCCTTGAGCAGCGTCCCAACAAACGCCCGATCAACTCCGACCTCCGGGAATCCGGAGCGATCGAG
CAGGATGCCGACGTCATCATGTTCGTTTACCGGGACGAGGTGTATCACCCGGAAACCGAGCACAAGGGTATTGCCGAGAT
CATCATCGGCAAGCAGCGTAACGGCCCTATCGGCACCACGCGCCTGGCGTTCATCGGCAAATACACGCGCTTCGAGAATC
TGGCACCGGGCAGTTACAACTTTGACGACGATTGA

Upstream 100 bases:

>100_bases
ATTGCTGCCAGAGGATTAACATCGGGCACGATCCTGTTTACAGGTCGTGCCCTTTGTCTTTGTGCCATCCCCATTCTGCA
AACCCCGAATTAAAGTGGCC

Downstream 100 bases:

>100_bases
TTTTGCCGCCAGATGCTGTTGCCGTTTCATCATGGCTCGTGCTGAGACGGTGGCAGCATCTATCGGTGCGTATCCGTGAT
TCGGGTTGGATACAATCGCC

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MNDISAPEQYDLQTAALKVPPHSIEAEQAVLGGLMLDNNAWERVLDQVSDGDFYRHDHRLIFRAIARLADQNSPIDVVTL
AEQLDKEGQTSQVGGLGYLSELAKNTPSVANIKAYAQIVRARATLRQLIGISTEIADSAFNPEGRTAEEILDEAERQIFQ
IAEARPKTGGPVSVNDLLTKAIDRIDTLFNSDNAITGLSTGYTDLDEKTSGLQPSDLIIVAGRPSMGKTTFAMNLVENAV
LRSDKVVLVYSLEMPGESLIMRMLSSLGRIDQTKVRAGQLDDDDWPRLTSAVNLLNDRKLFIDDTAGISPSEMRARTRRI
VREHGDIAMIMIDYLQLMQIPGSSGDNRTNEISEISRSLKALAKEFNCPVIALSQLNRSLEQRPNKRPINSDLRESGAIE
QDADVIMFVYRDEVYHPETEHKGIAEIIIGKQRNGPIGTTRLAFIGKYTRFENLAPGSYNFDDD

Sequences:

>Translated_464_residues
MNDISAPEQYDLQTAALKVPPHSIEAEQAVLGGLMLDNNAWERVLDQVSDGDFYRHDHRLIFRAIARLADQNSPIDVVTL
AEQLDKEGQTSQVGGLGYLSELAKNTPSVANIKAYAQIVRARATLRQLIGISTEIADSAFNPEGRTAEEILDEAERQIFQ
IAEARPKTGGPVSVNDLLTKAIDRIDTLFNSDNAITGLSTGYTDLDEKTSGLQPSDLIIVAGRPSMGKTTFAMNLVENAV
LRSDKVVLVYSLEMPGESLIMRMLSSLGRIDQTKVRAGQLDDDDWPRLTSAVNLLNDRKLFIDDTAGISPSEMRARTRRI
VREHGDIAMIMIDYLQLMQIPGSSGDNRTNEISEISRSLKALAKEFNCPVIALSQLNRSLEQRPNKRPINSDLRESGAIE
QDADVIMFVYRDEVYHPETEHKGIAEIIIGKQRNGPIGTTRLAFIGKYTRFENLAPGSYNFDDD
>Mature_464_residues
MNDISAPEQYDLQTAALKVPPHSIEAEQAVLGGLMLDNNAWERVLDQVSDGDFYRHDHRLIFRAIARLADQNSPIDVVTL
AEQLDKEGQTSQVGGLGYLSELAKNTPSVANIKAYAQIVRARATLRQLIGISTEIADSAFNPEGRTAEEILDEAERQIFQ
IAEARPKTGGPVSVNDLLTKAIDRIDTLFNSDNAITGLSTGYTDLDEKTSGLQPSDLIIVAGRPSMGKTTFAMNLVENAV
LRSDKVVLVYSLEMPGESLIMRMLSSLGRIDQTKVRAGQLDDDDWPRLTSAVNLLNDRKLFIDDTAGISPSEMRARTRRI
VREHGDIAMIMIDYLQLMQIPGSSGDNRTNEISEISRSLKALAKEFNCPVIALSQLNRSLEQRPNKRPINSDLRESGAIE
QDADVIMFVYRDEVYHPETEHKGIAEIIIGKQRNGPIGTTRLAFIGKYTRFENLAPGSYNFDDD

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=457, Percent_Identity=62.144420131291, Blast_Score=589, Evalue=1e-169,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 51416; Mature: 51416

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDISAPEQYDLQTAALKVPPHSIEAEQAVLGGLMLDNNAWERVLDQVSDGDFYRHDHRL
CCCCCCCCCCCCHHEEEECCCCCCCHHHHHHCCEEECCHHHHHHHHHCCCCCHHHHHHHH
IFRAIARLADQNSPIDVVTLAEQLDKEGQTSQVGGLGYLSELAKNTPSVANIKAYAQIVR
HHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHH
ARATLRQLIGISTEIADSAFNPEGRTAEEILDEAERQIFQIAEARPKTGGPVSVNDLLTK
HHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
AIDRIDTLFNSDNAITGLSTGYTDLDEKTSGLQPSDLIIVAGRPSMGKTTFAMNLVENAV
HHHHHHHHHCCCCCEEECCCCCCCCHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHH
LRSDKVVLVYSLEMPGESLIMRMLSSLGRIDQTKVRAGQLDDDDWPRLTSAVNLLNDRKL
HCCCCEEEEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCEE
FIDDTAGISPSEMRARTRRIVREHGDIAMIMIDYLQLMQIPGSSGDNRTNEISEISRSLK
EEECCCCCCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
ALAKEFNCPVIALSQLNRSLEQRPNKRPINSDLRESGAIEQDADVIMFVYRDEVYHPETE
HHHHHCCCCEEEHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCCCHH
HKGIAEIIIGKQRNGPIGTTRLAFIGKYTRFENLAPGSYNFDDD
CCCHHHHHEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNDISAPEQYDLQTAALKVPPHSIEAEQAVLGGLMLDNNAWERVLDQVSDGDFYRHDHRL
CCCCCCCCCCCCHHEEEECCCCCCCHHHHHHCCEEECCHHHHHHHHHCCCCCHHHHHHHH
IFRAIARLADQNSPIDVVTLAEQLDKEGQTSQVGGLGYLSELAKNTPSVANIKAYAQIVR
HHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHH
ARATLRQLIGISTEIADSAFNPEGRTAEEILDEAERQIFQIAEARPKTGGPVSVNDLLTK
HHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
AIDRIDTLFNSDNAITGLSTGYTDLDEKTSGLQPSDLIIVAGRPSMGKTTFAMNLVENAV
HHHHHHHHHCCCCCEEECCCCCCCCHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHH
LRSDKVVLVYSLEMPGESLIMRMLSSLGRIDQTKVRAGQLDDDDWPRLTSAVNLLNDRKL
HCCCCEEEEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCEE
FIDDTAGISPSEMRARTRRIVREHGDIAMIMIDYLQLMQIPGSSGDNRTNEISEISRSLK
EEECCCCCCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
ALAKEFNCPVIALSQLNRSLEQRPNKRPINSDLRESGAIEQDADVIMFVYRDEVYHPETE
HHHHHCCCCEEEHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCCCHH
HKGIAEIIIGKQRNGPIGTTRLAFIGKYTRFENLAPGSYNFDDD
CCCHHHHHEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]