Click here to switch to the map view.

The map label for this gene is amiC [H]

Identifier: 66043836

GI number: 66043836

Start: 646192

End: 647607

Strand: Direct

Name: amiC [H]

Synonym: Psyr_0569

Alternate gene names: 66043836

Gene position: 646192-647607 (Clockwise)

Preceding gene: 66043835

Following gene: 66043837

Centisome position: 10.6

GC content: 61.58

Gene sequence:

>1416_bases
ATGCGCATGCGCGCGCTGGTTACTGTAGTGGGTCTGCTGTTGATGGCCATGGCTGTCGAGGCGGTTGCCGCTACGCAAGT
ACGAAGTGTCCGCTTATGGCGAGCGCCGGATAACACACGTCTGGTCTTCGACCTGACGGGGCCGGTTCAGCACAGCCTGT
TTACCCTGACTTCGCCTGACCGTCTGGTGATCGATATCAATGGTGCGACGCTCGGCGGACCCTTGAACGTACCCACGGCC
AATACGCCGATCAGCAGCATGCGTTCTGCGCAGCGCACACCTACCGATCTGCGCGTGGTCATCGATCTGAAAAAGGGTGT
GACCCCGAAGAGCTTCACGCTGGCGCCCAACCAGCAATACGGCAACCGTCTGGTGGTCGACCTGTACGACAACGCCGCCG
ATGCCAACCCCACGCCGGTGATTCCCGACACCGCTGCCAACACCGCACCCGCAGTTCCGGTCAGCCCGGCCAAGCCTGAA
ATCAAGCTGACTCCGGTGCCGAATGGCAAGCGCGATATCGTCGTGGTGATCGATGCCGGGCACGGCGGGGAAGACCCTGG
TGCCTCGGGCGGCGCCGGTCAGAAAGAGAAAAACGTGGTGCTGTCGATTGCCAGGGAGTTGCAGCGCCAGGTGAATGCCG
AGAAAGGTTACCGCGCCGAACTGACGCGGACCGGCGACTACTTTATTCCGCTGCGCAAACGTACCGAGATCGCCCGCTCC
AAGGGCGCTGACCTGTTTGTGTCGATCCACGCCGACGCCGCGCCGTCTTCCGCTGCGTTCGGCGCATCGGTGTTCGCCCT
GTCTGATCGGGGTGCGACTTCCGAAACCGCCCGCTGGCTGGCGGACAGTGAAAACCGTTCTGACCTGATCGGTGGGGCCG
GCGCAGTCAGCCTCGATGACAAGGACCGCATGCTCGCGGGGGTGTTGCTGGACTTGTCCATGACCGCCTCGCTGTCTTCG
AGCCTGAACGTTGGCCAGAAGGTATTGAGCAATATTGGCCGCGTGACCTCGCTGCACAAGGCACGCGTCGAGCAGGCGGG
CTTCATGGTGCTCAAGTCGCCGGATATCCCGTCGATTCTGGTGGAAACCGGCTTTATCTCCAACGCCAATGAGGCCAACA
AGCTCGGTTCTGCGTCGCATCAGCAGGCGCTGGCGCGTTCGATCACGTCGGGTGTCAAGCAGTTCTTCCAGCAGAACCCG
CCGCAGGGCACCTACATTGCCTGGCTGCGTGACAACGGCAAGCTGGCCCAGGGGCCGCGCACTCATGTCGTTCGTTCCGG
CGAAACCCTGGCCATGCTTGCTGCACGCTATGACATGAACATCGCCACTTTGCGCAGCGCCAACAATCTGAAGTCCGATG
AATTGAAGATCGGCCAGGATCTGCGTATTCCAAGTTCCGAGGTGGCGACTCAGTAA

Upstream 100 bases:

>100_bases
ACGGTGAAGGTCGGTCCGTGATATTGAGCCCGCTGGGCTCGCGTGGCGAACAGTGGTGTGCCACTTTGGCTTTGGAATTC
AAATAGATATGGGGTTAGGT

Downstream 100 bases:

>100_bases
TGACCGATCTCCTGCTCGACGGCGCCGAAGCCGATAACGCTCAGACTGCCCTGAACGCAGCACGCATCGAACTGCTCAGT
CCGCGTCTGGCCAACCAGAT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MRMRALVTVVGLLLMAMAVEAVAATQVRSVRLWRAPDNTRLVFDLTGPVQHSLFTLTSPDRLVIDINGATLGGPLNVPTA
NTPISSMRSAQRTPTDLRVVIDLKKGVTPKSFTLAPNQQYGNRLVVDLYDNAADANPTPVIPDTAANTAPAVPVSPAKPE
IKLTPVPNGKRDIVVVIDAGHGGEDPGASGGAGQKEKNVVLSIARELQRQVNAEKGYRAELTRTGDYFIPLRKRTEIARS
KGADLFVSIHADAAPSSAAFGASVFALSDRGATSETARWLADSENRSDLIGGAGAVSLDDKDRMLAGVLLDLSMTASLSS
SLNVGQKVLSNIGRVTSLHKARVEQAGFMVLKSPDIPSILVETGFISNANEANKLGSASHQQALARSITSGVKQFFQQNP
PQGTYIAWLRDNGKLAQGPRTHVVRSGETLAMLAARYDMNIATLRSANNLKSDELKIGQDLRIPSSEVATQ

Sequences:

>Translated_471_residues
MRMRALVTVVGLLLMAMAVEAVAATQVRSVRLWRAPDNTRLVFDLTGPVQHSLFTLTSPDRLVIDINGATLGGPLNVPTA
NTPISSMRSAQRTPTDLRVVIDLKKGVTPKSFTLAPNQQYGNRLVVDLYDNAADANPTPVIPDTAANTAPAVPVSPAKPE
IKLTPVPNGKRDIVVVIDAGHGGEDPGASGGAGQKEKNVVLSIARELQRQVNAEKGYRAELTRTGDYFIPLRKRTEIARS
KGADLFVSIHADAAPSSAAFGASVFALSDRGATSETARWLADSENRSDLIGGAGAVSLDDKDRMLAGVLLDLSMTASLSS
SLNVGQKVLSNIGRVTSLHKARVEQAGFMVLKSPDIPSILVETGFISNANEANKLGSASHQQALARSITSGVKQFFQQNP
PQGTYIAWLRDNGKLAQGPRTHVVRSGETLAMLAARYDMNIATLRSANNLKSDELKIGQDLRIPSSEVATQ
>Mature_471_residues
MRMRALVTVVGLLLMAMAVEAVAATQVRSVRLWRAPDNTRLVFDLTGPVQHSLFTLTSPDRLVIDINGATLGGPLNVPTA
NTPISSMRSAQRTPTDLRVVIDLKKGVTPKSFTLAPNQQYGNRLVVDLYDNAADANPTPVIPDTAANTAPAVPVSPAKPE
IKLTPVPNGKRDIVVVIDAGHGGEDPGASGGAGQKEKNVVLSIARELQRQVNAEKGYRAELTRTGDYFIPLRKRTEIARS
KGADLFVSIHADAAPSSAAFGASVFALSDRGATSETARWLADSENRSDLIGGAGAVSLDDKDRMLAGVLLDLSMTASLSS
SLNVGQKVLSNIGRVTSLHKARVEQAGFMVLKSPDIPSILVETGFISNANEANKLGSASHQQALARSITSGVKQFFQQNP
PQGTYIAWLRDNGKLAQGPRTHVVRSGETLAMLAARYDMNIATLRSANNLKSDELKIGQDLRIPSSEVATQ

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=394, Percent_Identity=40.8629441624365, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1790611, Length=436, Percent_Identity=36.697247706422, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1788776, Length=241, Percent_Identity=49.3775933609959, Blast_Score=211, Evalue=7e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 50122; Mature: 50122

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMRALVTVVGLLLMAMAVEAVAATQVRSVRLWRAPDNTRLVFDLTGPVQHSLFTLTSPD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCCCCEEEECCCC
RLVIDINGATLGGPLNVPTANTPISSMRSAQRTPTDLRVVIDLKKGVTPKSFTLAPNQQY
EEEEEECCCEECCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCEEEECCCHHH
GNRLVVDLYDNAADANPTPVIPDTAANTAPAVPVSPAKPEIKLTPVPNGKRDIVVVIDAG
CCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECC
HGGEDPGASGGAGQKEKNVVLSIARELQRQVNAEKGYRAELTRTGDYFIPLRKRTEIARS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEECHHHHHHHHC
KGADLFVSIHADAAPSSAAFGASVFALSDRGATSETARWLADSENRSDLIGGAGAVSLDD
CCCEEEEEEECCCCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCHHCCCCCEEEECC
KDRMLAGVLLDLSMTASLSSSLNVGQKVLSNIGRVTSLHKARVEQAGFMVLKSPDIPSIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEE
VETGFISNANEANKLGSASHQQALARSITSGVKQFFQQNPPQGTYIAWLRDNGKLAQGPR
EECCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCC
THVVRSGETLAMLAARYDMNIATLRSANNLKSDELKIGQDLRIPSSEVATQ
CEEEECCCCEEEEEHHHCCCEEEECCCCCCCCCCEECCCCCCCCCHHHCCC
>Mature Secondary Structure
MRMRALVTVVGLLLMAMAVEAVAATQVRSVRLWRAPDNTRLVFDLTGPVQHSLFTLTSPD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCCCCEEEECCCC
RLVIDINGATLGGPLNVPTANTPISSMRSAQRTPTDLRVVIDLKKGVTPKSFTLAPNQQY
EEEEEECCCEECCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCEEEECCCHHH
GNRLVVDLYDNAADANPTPVIPDTAANTAPAVPVSPAKPEIKLTPVPNGKRDIVVVIDAG
CCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECC
HGGEDPGASGGAGQKEKNVVLSIARELQRQVNAEKGYRAELTRTGDYFIPLRKRTEIARS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEECHHHHHHHHC
KGADLFVSIHADAAPSSAAFGASVFALSDRGATSETARWLADSENRSDLIGGAGAVSLDD
CCCEEEEEEECCCCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCHHCCCCCEEEECC
KDRMLAGVLLDLSMTASLSSSLNVGQKVLSNIGRVTSLHKARVEQAGFMVLKSPDIPSIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEE
VETGFISNANEANKLGSASHQQALARSITSGVKQFFQQNPPQGTYIAWLRDNGKLAQGPR
EECCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCC
THVVRSGETLAMLAARYDMNIATLRSANNLKSDELKIGQDLRIPSSEVATQ
CEEEECCCCEEEEEHHHCCCEEEECCCCCCCCCCEECCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]