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The map label for this gene is pilJ [H]

Identifier: 66043758

GI number: 66043758

Start: 526901

End: 528943

Strand: Direct

Name: pilJ [H]

Synonym: Psyr_0491

Alternate gene names: 66043758

Gene position: 526901-528943 (Clockwise)

Preceding gene: 66043757

Following gene: 66043759

Centisome position: 8.65

GC content: 61.67

Gene sequence:

>2043_bases
ATGAGTAGTACAGACCGGTCAAGGGAAGGCGCAAGAAGCCGGACGCAGATTATTCTGCTGTTCGTGGCGCTTATCGTTTT
CATCATGTTGCTGTTCGCCAACTTCGCCTACCTCAACACTCAGTCCAACTACGACAAACAGTACATCGGGCATGCCGGTG
AGCTGCGCGTGCTGTCGCAACGCATCGCGAAAAACGCCACCGAAGCGGCTGCCGGCAAAACCCAGGCGTTCAAATTGCTG
GCCGATGCGCGCAACGATTTCGACGTGCGCTGGGGCTACCTGAGAAAAGGCGACCCGGCCACCGGCCTGCCTGCGGCACC
CGAGCTGATACGCGACGAATTGCGCACCGTGCAACGTGACTGGGAAGGGCTGCGCAAGAGCACCGACGTGATTCTGGCCA
GTGAACAGACCGTCCTGTCGTTGCATCAGGTGGCGGCAACGCTGGCCGAAACCATTCCGCAATTGCAGGCCGAATACGAA
AAAGTCGTCGAGAGCCTGCTGCAAAGCCGTGCTCCTGCCGCACAGGTGGTGGTTGCGCAGCGCCAAGCGCTGCTCGCAGA
ACGGATTCTCGGCTCGGTCAATACCGTGCTGGCCGGTGATGAAACGGCTGTGCAGGCCGCCGATGCCTTTGGCCGCGACG
CCAGCCAGTTCGGTCGTGTGCTCAACGGCATGCTGGAGGGCAACGCCACGCTGCGCATATCCCAGGTCGAAGACCGCGAT
GCGCGCGCGCGGCTGGCGGAGATCGCCGAACTGTTCGAATTCGTCTCAGGCTCGGTGGACGAGATTCTCGAGACTTCGCC
CGAGCTGTATCAGGTGCGCGAGGCGTCGGGCAACATCTTCAACACCTCACAGACCCTGCTGGATGAAACCTCGGTACTCG
CCAACAGCCTGGAAAACCTCGCCAAGCGCCGCACCGTGAACACCGTCGGCGGCTATGTGCTGGGCTTGCTGGCACTGGCG
TCGATCATCCTCATCGGTCTGGTCATGGTGCGCGAGACCAATCGCCAGCTGCGCGAAACCGCGCAGAAAAGCGAGCGCAA
CCAGACAGCGATCATGCGCCTGCTGGACGAGATCGAGAACCTCGCCGACGGCGACCTGACGGTCACCGCCTCGGTGACCG
AGGACTTCACCGGGGCGATTGCCGACTCCATCAATTATTCCATTGACCAGTTGCGCGAGCTGGTGGTGACCATCAACCTG
ACCGCCGAGCAAGTGGCGGCTGCGGTGACCGAAACCCAGGCGACGGCCATGCAATTGTCGGCTGCCTCTGAGCACCAGGC
GTTGCAGATCAGTGCCGCTTCGACGGCGATCAACGACATGGCCGCGTCCATCGATCAGGTTTCGACCAACGCCTCGGAGT
CCTCGGCCGTGGCGGAGCGCTCCGTGACAATTGCCAATAAAGGCAACGAGGTCGTGCAAAACACCATTCACGGCATGGAC
AACATTCGCGAGCAGATTCAAGACACCTCCAAGCGCATCAAGCGTCTGGGCGAGTCCTCCCAGGAGATTGGCGACATCGT
CAGCCTGATCGACGACATTGCCGACCAGACCAATATCCTGGCCTTGAACGCCGCCATTCAGGCGTCGATGGCCGGTGATG
CCGGACGAGGGTTTGCCGTGGTGGCTGACGAAGTGCAGCGCCTGGCCGAGCGTTCGTCGTCTGCGACCAAGCAGATCGAA
ACGCTGGTCCGGGCCATCCAGAACGACACCAACGAGGCGGTTATCTCCATGGAGCAGACCACCAGCGAAGTGGTGCGCGG
CGCCCGGCTGGCGCAGGACGCCGGTGTGGCGCTGGGGGAAATCGAAGGGGTGTCCAGGGTGCTGGCCGAACTGATCGAAA
GTATCACCGACGCTGCCCACCAGCAGGCCGAGTCTGCCGGGCAGATTTCGCAGATCATGACCGTCATTCAGCAGACCACT
TCGCAGACCACCTCGGGCACATCGGCCACCGCCGAAAGCATCGGCAACCTGGCGAAAATGGCCAGCGAAATGCGCCGCTC
GGTATCGGGTTTTGCCCTGCCGTCATCGAAGAAAGCCGGATGA

Upstream 100 bases:

>100_bases
GCCCATGGGCGCTGGTGCAGTCTGCGGATTTCATGGACCTGGCTTCCTAGCATGTCGGGCCTGGTGTGTTTCACAATTGA
TCCAGGCGGGGGCCTGATTA

Downstream 100 bases:

>100_bases
TGTACCGCACATGAACAACCTTGATTGGGTAACGGACGGTACTCGTCATGGCTGATCGTCACGATTACGTAGCTCTTGAA
TGGCTGAAGGGCGAAATCGC

Product: chemotaxis sensory transducer

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 680; Mature: 679

Protein sequence:

>680_residues
MSSTDRSREGARSRTQIILLFVALIVFIMLLFANFAYLNTQSNYDKQYIGHAGELRVLSQRIAKNATEAAAGKTQAFKLL
ADARNDFDVRWGYLRKGDPATGLPAAPELIRDELRTVQRDWEGLRKSTDVILASEQTVLSLHQVAATLAETIPQLQAEYE
KVVESLLQSRAPAAQVVVAQRQALLAERILGSVNTVLAGDETAVQAADAFGRDASQFGRVLNGMLEGNATLRISQVEDRD
ARARLAEIAELFEFVSGSVDEILETSPELYQVREASGNIFNTSQTLLDETSVLANSLENLAKRRTVNTVGGYVLGLLALA
SIILIGLVMVRETNRQLRETAQKSERNQTAIMRLLDEIENLADGDLTVTASVTEDFTGAIADSINYSIDQLRELVVTINL
TAEQVAAAVTETQATAMQLSAASEHQALQISAASTAINDMAASIDQVSTNASESSAVAERSVTIANKGNEVVQNTIHGMD
NIREQIQDTSKRIKRLGESSQEIGDIVSLIDDIADQTNILALNAAIQASMAGDAGRGFAVVADEVQRLAERSSSATKQIE
TLVRAIQNDTNEAVISMEQTTSEVVRGARLAQDAGVALGEIEGVSRVLAELIESITDAAHQQAESAGQISQIMTVIQQTT
SQTTSGTSATAESIGNLAKMASEMRRSVSGFALPSSKKAG

Sequences:

>Translated_680_residues
MSSTDRSREGARSRTQIILLFVALIVFIMLLFANFAYLNTQSNYDKQYIGHAGELRVLSQRIAKNATEAAAGKTQAFKLL
ADARNDFDVRWGYLRKGDPATGLPAAPELIRDELRTVQRDWEGLRKSTDVILASEQTVLSLHQVAATLAETIPQLQAEYE
KVVESLLQSRAPAAQVVVAQRQALLAERILGSVNTVLAGDETAVQAADAFGRDASQFGRVLNGMLEGNATLRISQVEDRD
ARARLAEIAELFEFVSGSVDEILETSPELYQVREASGNIFNTSQTLLDETSVLANSLENLAKRRTVNTVGGYVLGLLALA
SIILIGLVMVRETNRQLRETAQKSERNQTAIMRLLDEIENLADGDLTVTASVTEDFTGAIADSINYSIDQLRELVVTINL
TAEQVAAAVTETQATAMQLSAASEHQALQISAASTAINDMAASIDQVSTNASESSAVAERSVTIANKGNEVVQNTIHGMD
NIREQIQDTSKRIKRLGESSQEIGDIVSLIDDIADQTNILALNAAIQASMAGDAGRGFAVVADEVQRLAERSSSATKQIE
TLVRAIQNDTNEAVISMEQTTSEVVRGARLAQDAGVALGEIEGVSRVLAELIESITDAAHQQAESAGQISQIMTVIQQTT
SQTTSGTSATAESIGNLAKMASEMRRSVSGFALPSSKKAG
>Mature_679_residues
SSTDRSREGARSRTQIILLFVALIVFIMLLFANFAYLNTQSNYDKQYIGHAGELRVLSQRIAKNATEAAAGKTQAFKLLA
DARNDFDVRWGYLRKGDPATGLPAAPELIRDELRTVQRDWEGLRKSTDVILASEQTVLSLHQVAATLAETIPQLQAEYEK
VVESLLQSRAPAAQVVVAQRQALLAERILGSVNTVLAGDETAVQAADAFGRDASQFGRVLNGMLEGNATLRISQVEDRDA
RARLAEIAELFEFVSGSVDEILETSPELYQVREASGNIFNTSQTLLDETSVLANSLENLAKRRTVNTVGGYVLGLLALAS
IILIGLVMVRETNRQLRETAQKSERNQTAIMRLLDEIENLADGDLTVTASVTEDFTGAIADSINYSIDQLRELVVTINLT
AEQVAAAVTETQATAMQLSAASEHQALQISAASTAINDMAASIDQVSTNASESSAVAERSVTIANKGNEVVQNTIHGMDN
IREQIQDTSKRIKRLGESSQEIGDIVSLIDDIADQTNILALNAAIQASMAGDAGRGFAVVADEVQRLAERSSSATKQIET
LVRAIQNDTNEAVISMEQTTSEVVRGARLAQDAGVALGEIEGVSRVLAELIESITDAAHQQAESAGQISQIMTVIQQTTS
QTTSGTSATAESIGNLAKMASEMRRSVSGFALPSSKKAG

Specific function: May be a part of a signal-transduction system that regulates twitching motility by controlling pilus function (extension and retraction) [H]

COG id: COG0840

COG function: function code NT; Methyl-accepting chemotaxis protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI2367378, Length=292, Percent_Identity=31.5068493150685, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788195, Length=331, Percent_Identity=29.9093655589124, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1787690, Length=391, Percent_Identity=29.156010230179, Blast_Score=114, Evalue=3e-26,
Organism=Escherichia coli, GI1788194, Length=284, Percent_Identity=32.0422535211268, Blast_Score=113, Evalue=5e-26,
Organism=Escherichia coli, GI1789453, Length=191, Percent_Identity=36.1256544502618, Blast_Score=94, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 73011; Mature: 72880

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTDRSREGARSRTQIILLFVALIVFIMLLFANFAYLNTQSNYDKQYIGHAGELRVLSQ
CCCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHH
RIAKNATEAAAGKTQAFKLLADARNDFDVRWGYLRKGDPATGLPAAPELIRDELRTVQRD
HHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
WEGLRKSTDVILASEQTVLSLHQVAATLAETIPQLQAEYEKVVESLLQSRAPAAQVVVAQ
HHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
RQALLAERILGSVNTVLAGDETAVQAADAFGRDASQFGRVLNGMLEGNATLRISQVEDRD
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCHH
ARARLAEIAELFEFVSGSVDEILETSPELYQVREASGNIFNTSQTLLDETSVLANSLENL
HHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHH
AKRRTVNTVGGYVLGLLALASIILIGLVMVRETNRQLRETAQKSERNQTAIMRLLDEIEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LADGDLTVTASVTEDFTGAIADSINYSIDQLRELVVTINLTAEQVAAAVTETQATAMQLS
CCCCCEEEEEECHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AASEHQALQISAASTAINDMAASIDQVSTNASESSAVAERSVTIANKGNEVVQNTIHGMD
HCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCEEEECCCHHHHHHHHHHHH
NIREQIQDTSKRIKRLGESSQEIGDIVSLIDDIADQTNILALNAAIQASMAGDAGRGFAV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCHHH
VADEVQRLAERSSSATKQIETLVRAIQNDTNEAVISMEQTTSEVVRGARLAQDAGVALGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEHHHHHHHHHHHHHHHHHHCCHHHH
IEGVSRVLAELIESITDAAHQQAESAGQISQIMTVIQQTTSQTTSGTSATAESIGNLAKM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
ASEMRRSVSGFALPSSKKAG
HHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SSTDRSREGARSRTQIILLFVALIVFIMLLFANFAYLNTQSNYDKQYIGHAGELRVLSQ
CCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHH
RIAKNATEAAAGKTQAFKLLADARNDFDVRWGYLRKGDPATGLPAAPELIRDELRTVQRD
HHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
WEGLRKSTDVILASEQTVLSLHQVAATLAETIPQLQAEYEKVVESLLQSRAPAAQVVVAQ
HHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
RQALLAERILGSVNTVLAGDETAVQAADAFGRDASQFGRVLNGMLEGNATLRISQVEDRD
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCHH
ARARLAEIAELFEFVSGSVDEILETSPELYQVREASGNIFNTSQTLLDETSVLANSLENL
HHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHH
AKRRTVNTVGGYVLGLLALASIILIGLVMVRETNRQLRETAQKSERNQTAIMRLLDEIEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LADGDLTVTASVTEDFTGAIADSINYSIDQLRELVVTINLTAEQVAAAVTETQATAMQLS
CCCCCEEEEEECHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AASEHQALQISAASTAINDMAASIDQVSTNASESSAVAERSVTIANKGNEVVQNTIHGMD
HCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCEEEECCCHHHHHHHHHHHH
NIREQIQDTSKRIKRLGESSQEIGDIVSLIDDIADQTNILALNAAIQASMAGDAGRGFAV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCHHH
VADEVQRLAERSSSATKQIETLVRAIQNDTNEAVISMEQTTSEVVRGARLAQDAGVALGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEHHHHHHHHHHHHHHHHHHCCHHHH
IEGVSRVLAELIESITDAAHQQAESAGQISQIMTVIQQTTSQTTSGTSATAESIGNLAKM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
ASEMRRSVSGFALPSSKKAG
HHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7908398; 10984043 [H]