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The map label for this gene is czcO [H]

Identifier: 62390391

GI number: 62390391

Start: 1593405

End: 1594433

Strand: Reverse

Name: czcO [H]

Synonym: cg1703

Alternate gene names: 62390391

Gene position: 1594433-1593405 (Counterclockwise)

Preceding gene: 62390392

Following gene: 62390389

Centisome position: 48.57

GC content: 50.15

Gene sequence:

>1029_bases
ATGATGAGTCGTTTGCAAACTGAAGGCGCCGGAAACGATCATGACCATTACGAAGCTGTAATAATTGGTGGAGGCCAAGC
TGGCCTGGCTACCGCTTATTATCTCATGCGCGCAGGAGTGCAGACTCTGATTTTGGATGACCAACCTGCCTCCGGAGGTG
CATGGCTGCATGTGTGGCCTTCGCTGCATTTGTTTTCGACTTCAGATTTTTCTAATCTTCCAGGTTGGCCGATGCCCAAA
TTTGACGGGTTTCCAACATCGTCTCATGTCGTAGATTACCTGACAGAATATGAACGGCGTTATGAAATTCCAGTTAGTCG
ATCAGTTTCTGTAGATAAGGTTGATTTTGTAAATGGTGCCTTTCATTTACAATCTGGAACTCGATCCTGGATAGCTAAAA
ACGTGGTTGCCGCTACCGGTACTTGGTCGGCGCCATTTGTCCCAACTTTTCCGGGAAAATTCTCTGGAACTCATTGGCAC
TCTTCAAATTATCCGGGCGTTCACCCCTTCGTAGGTCAACGCATTGCAATCGTTGGAGGTGCAAATTCCGGAGCTCAAAT
TGCAGCAGAGCTGAGTGAAGTGAGTGAGGTGACATGGTTCACCCGCGATAAACCACGGTGGATGCCAGATGACGTTGATG
GTAGGGAATTGTTTAGGCGTAACCGTTTGAGAGCTCTTGCTATTCAACGAGGTGAACGTGACCCAGGGCCAGATTCCGAG
CTCGGCGACATTGTCATGCTTCCGGAGGTGCGCAGGGCTCGAGACTCCGGAAAGCTATTTGCCACTCCGATGTTTGGGTC
ACTCGACGAGATACGTGCTGATCACCTAATTTGGTGCACAGGGTTTCGGCCGGCGATTAGGCCAGTTCGTCAACTTCTCA
AACACGGACAACCAAAGGTTCCTGGTCTTTATTTAGTAGGCTACGGAGATTGGACGGGACCTGGGTCTGCGACTATCACA
GGGGTCGGGCTTTATGCCAAGCGAGCAGCCAAAGAGATTGCCGCGTCAGTCGGCAAAGTCGTTAAATAG

Upstream 100 bases:

>100_bases
TTTTGGATCGAGTGCCTGAAGGTCGTGTGGTGTTGCATCGAGTACGGCCGGAATTATTTGCGGAATTGCGAACGGTATTG
CAGATCGGCTCAATGGAATT

Downstream 100 bases:

>100_bases
TTTGAAGGCTAAGAACTTAATGTTAAAGCGAAAATTGTTTTGACACCTCAACTAATGCAGCGATGCGTTCTTTCCAGAAT
GCTTTCATGACAGGGATGCT

Product: FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: NADP+; reduced thioredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MMSRLQTEGAGNDHDHYEAVIIGGGQAGLATAYYLMRAGVQTLILDDQPASGGAWLHVWPSLHLFSTSDFSNLPGWPMPK
FDGFPTSSHVVDYLTEYERRYEIPVSRSVSVDKVDFVNGAFHLQSGTRSWIAKNVVAATGTWSAPFVPTFPGKFSGTHWH
SSNYPGVHPFVGQRIAIVGGANSGAQIAAELSEVSEVTWFTRDKPRWMPDDVDGRELFRRNRLRALAIQRGERDPGPDSE
LGDIVMLPEVRRARDSGKLFATPMFGSLDEIRADHLIWCTGFRPAIRPVRQLLKHGQPKVPGLYLVGYGDWTGPGSATIT
GVGLYAKRAAKEIAASVGKVVK

Sequences:

>Translated_342_residues
MMSRLQTEGAGNDHDHYEAVIIGGGQAGLATAYYLMRAGVQTLILDDQPASGGAWLHVWPSLHLFSTSDFSNLPGWPMPK
FDGFPTSSHVVDYLTEYERRYEIPVSRSVSVDKVDFVNGAFHLQSGTRSWIAKNVVAATGTWSAPFVPTFPGKFSGTHWH
SSNYPGVHPFVGQRIAIVGGANSGAQIAAELSEVSEVTWFTRDKPRWMPDDVDGRELFRRNRLRALAIQRGERDPGPDSE
LGDIVMLPEVRRARDSGKLFATPMFGSLDEIRADHLIWCTGFRPAIRPVRQLLKHGQPKVPGLYLVGYGDWTGPGSATIT
GVGLYAKRAAKEIAASVGKVVK
>Mature_342_residues
MMSRLQTEGAGNDHDHYEAVIIGGGQAGLATAYYLMRAGVQTLILDDQPASGGAWLHVWPSLHLFSTSDFSNLPGWPMPK
FDGFPTSSHVVDYLTEYERRYEIPVSRSVSVDKVDFVNGAFHLQSGTRSWIAKNVVAATGTWSAPFVPTFPGKFSGTHWH
SSNYPGVHPFVGQRIAIVGGANSGAQIAAELSEVSEVTWFTRDKPRWMPDDVDGRELFRRNRLRALAIQRGERDPGPDSE
LGDIVMLPEVRRARDSGKLFATPMFGSLDEIRADHLIWCTGFRPAIRPVRQLLKHGQPKVPGLYLVGYGDWTGPGSATIT
GVGLYAKRAAKEIAASVGKVVK

Specific function: Involved in potassium and divalent cation transport. Enhances the transport activity of the cation/potassium transporter CzcD [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 808 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR000103 [H]

Pfam domain/function: PF07992 Pyr_redox_2 [H]

EC number: 1.8.1.9 [C]

Molecular weight: Translated: 37502; Mature: 37502

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSRLQTEGAGNDHDHYEAVIIGGGQAGLATAYYLMRAGVQTLILDDQPASGGAWLHVWP
CCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEECC
SLHLFSTSDFSNLPGWPMPKFDGFPTSSHVVDYLTEYERRYEIPVSRSVSVDKVDFVNGA
CEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCE
FHLQSGTRSWIAKNVVAATGTWSAPFVPTFPGKFSGTHWHSSNYPGVHPFVGQRIAIVGG
EEECCCCHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEC
ANSGAQIAAELSEVSEVTWFTRDKPRWMPDDVDGRELFRRNRLRALAIQRGERDPGPDSE
CCCCHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHEEHHHCCCCCCCCCCC
LGDIVMLPEVRRARDSGKLFATPMFGSLDEIRADHLIWCTGFRPAIRPVRQLLKHGQPKV
CCCEEECCCHHHHCCCCCEEEECCCCCHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCC
PGLYLVGYGDWTGPGSATITGVGLYAKRAAKEIAASVGKVVK
CEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMSRLQTEGAGNDHDHYEAVIIGGGQAGLATAYYLMRAGVQTLILDDQPASGGAWLHVWP
CCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEECC
SLHLFSTSDFSNLPGWPMPKFDGFPTSSHVVDYLTEYERRYEIPVSRSVSVDKVDFVNGA
CEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCE
FHLQSGTRSWIAKNVVAATGTWSAPFVPTFPGKFSGTHWHSSNYPGVHPFVGQRIAIVGG
EEECCCCHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEC
ANSGAQIAAELSEVSEVTWFTRDKPRWMPDDVDGRELFRRNRLRALAIQRGERDPGPDSE
CCCCHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHEEHHHCCCCCCCCCCC
LGDIVMLPEVRRARDSGKLFATPMFGSLDEIRADHLIWCTGFRPAIRPVRQLLKHGQPKV
CCCEEECCCHHHHCCCCCEEEECCCCCHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCC
PGLYLVGYGDWTGPGSATITGVGLYAKRAAKEIAASVGKVVK
CEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: NA

Kcat value (1/min): 1365 [C]

Specific activity: NA

Km value (mM): 0.125 {thioredoxin} 0.008 {NADPH}} 0.002 {thioredoxin}} 0.0017 {thioredoxin}} [C]

Substrates: NADPH; oxidized thioredoxin [C]

Specific reaction: NADPH + oxidized thioredoxin = NADP+ + reduced thioredoxin [C]

General reaction: Redox reaction [C]

Inhibitor: Ca2+; N-Ethyl maleimide; NADP+ [C]

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9099864; 9308178; 9384377 [H]