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The map label for this gene is aspA

Identifier: 62390385

GI number: 62390385

Start: 1585377

End: 1586957

Strand: Reverse

Name: aspA

Synonym: cg1697

Alternate gene names: 62390385

Gene position: 1586957-1585377 (Counterclockwise)

Preceding gene: 62390386

Following gene: 62390384

Centisome position: 48.34

GC content: 53.38

Gene sequence:

>1581_bases
ATGTCTAAGACGAGCAACAAGTCTTCAGCAGACTCAAAGAATGACGCAAAAGCCGAAGACATTGTGAACGGCGAGAACCA
AATCGCCACGAATGAGTCGCAGTCTTCAGACAGCGCTGCAGTTTCGGAACGTGTCGTCGAACCAAAAACCACGGTTCAGA
AAAAGTTCCGAATCGAATCGGATCTGCTTGGTGAACTTCAGATCCCATCCCACGCATATTACGGGGTGCACACCCTTCGT
GCGGTGGACAACTTCCAAATCTCACGAACCACCATCAACCACGTCCCAGATTTCATTCGCGGCATGGTCCAGGTGAAAAA
GGCCGCAGCTTTAGCAAACCGCCGACTGCACACACTTCCAGCACAAAAAGCAGAAGCAATTGTCTGGGCTTGTGATCAGA
TCCTCATTGAGGAACGCTGTATGGATCAGTTCCCCATCGATGTGTTCCAGGGTGGCGCAGGTACCTCACTGAACATGAAC
ACCAACGAGGTTGTTGCCAACCTTGCACTTGAGTTCTTAGGCCATGAAAAGGGCGAGTACCACATCCTGCACCCCATGGA
TGATGTGAACATGTCCCAGTCCACCAACGATTCCTACCCAACTGGTTTCCGCCTGGGCATTTACGCTGGACTGCAGACCC
TCATCGCTGAAATTGATGAGCTTCAGGTTGCGTTCCGCCACAAGGGCAATGAGTTTGTCGACATCATCAAGATGGGCCGC
ACCCAGTTGCAGGATGCTGTTCCCATGAGCTTGGGCGAAGAGTTCCGAGCATTCGCGCACAACCTCGCAGAAGAGCAGAC
CGTGCTGCGTGAAGCTGCCAACCGTCTCCTCGAGGTCAATCTTGGTGCAACCGCAATCGGTACTGGTGTGAACACTCCAG
CAGGCTACCGCCACCAGGTTGTCGCTGCTCTGTCTGAGGTCACCGGACTGGAACTAAAGTCCGCACGTGATCTCATCGAG
GCTACCTCTGACACCGGTGCATATGTTCATGCGCACTCCGCAATCAAGCGTGCAGCCATGAAACTGTCCAAGATCTGTAA
CGATCTACGTCTGCTGTCTTCTGGTCCTCGTGCTGGCTTGAACGAAATCAACCTGCCACCACGCCAGGCTGGTTCCTCCA
TCATGCCAGCCAAGGTCAACCCAGTGATCCCAGAAGTGGTCAACCAGGTCTGCTTCAAGGTCTTCGGTAACGATCTCACC
GTCACCATGGCTGCGGAAGCTGGCCAGTTGCAGCTCAACGTCATGGAGCCAGTCATTGGCGAATCCCTCTTCCAGTCACT
GCGCATCCTGGGCAATGCAGCCAAGACTTTGCGTGAGAAGTGCGTCGTAGGAATCACCGCCAACGCTGATGTTTGCCGTG
CTTACGTTGATAACTCCATCGGGATTATCACTTACCTGAACCCATTCCTGGGCCACGACATTGGAGATCAGATCGGTAAG
GAAGCAGCCGAAACTGGTCGACCAGTGCGTGAACTCATCCTGGAAAAGAAGCTCATGGATGAAAAGACGCTCGAGGCAGT
CCTGTCCAAGGAGAACCTCATGCACCCAATGTTCCGCGGAAGGCTCTACTTGGAGAACTAA

Upstream 100 bases:

>100_bases
CCCCCGAAAATGTAGTGGGTAGTTCCACACTGCCTCTTACAAGTACGTAGGATAATCCACAGCACCATTGTGATTTCCTT
CAACTTGTGAGAGGCAGTAC

Downstream 100 bases:

>100_bases
TCCAAGATCTCGTCTGATACCTGGGCAATTAGGTTGAGAGTTCATCTCACCTGGTTGCCCAGGTTTTTTCGTGGGTGAAT
TCAGCAGGCGCCTGTTTAAG

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSKTSNKSSADSKNDAKAEDIVNGENQIATNESQSSDSAAVSERVVEPKTTVQKKFRIESDLLGELQIPSHAYYGVHTLR
AVDNFQISRTTINHVPDFIRGMVQVKKAAALANRRLHTLPAQKAEAIVWACDQILIEERCMDQFPIDVFQGGAGTSLNMN
TNEVVANLALEFLGHEKGEYHILHPMDDVNMSQSTNDSYPTGFRLGIYAGLQTLIAEIDELQVAFRHKGNEFVDIIKMGR
TQLQDAVPMSLGEEFRAFAHNLAEEQTVLREAANRLLEVNLGATAIGTGVNTPAGYRHQVVAALSEVTGLELKSARDLIE
ATSDTGAYVHAHSAIKRAAMKLSKICNDLRLLSSGPRAGLNEINLPPRQAGSSIMPAKVNPVIPEVVNQVCFKVFGNDLT
VTMAAEAGQLQLNVMEPVIGESLFQSLRILGNAAKTLREKCVVGITANADVCRAYVDNSIGIITYLNPFLGHDIGDQIGK
EAAETGRPVRELILEKKLMDEKTLEAVLSKENLMHPMFRGRLYLEN

Sequences:

>Translated_526_residues
MSKTSNKSSADSKNDAKAEDIVNGENQIATNESQSSDSAAVSERVVEPKTTVQKKFRIESDLLGELQIPSHAYYGVHTLR
AVDNFQISRTTINHVPDFIRGMVQVKKAAALANRRLHTLPAQKAEAIVWACDQILIEERCMDQFPIDVFQGGAGTSLNMN
TNEVVANLALEFLGHEKGEYHILHPMDDVNMSQSTNDSYPTGFRLGIYAGLQTLIAEIDELQVAFRHKGNEFVDIIKMGR
TQLQDAVPMSLGEEFRAFAHNLAEEQTVLREAANRLLEVNLGATAIGTGVNTPAGYRHQVVAALSEVTGLELKSARDLIE
ATSDTGAYVHAHSAIKRAAMKLSKICNDLRLLSSGPRAGLNEINLPPRQAGSSIMPAKVNPVIPEVVNQVCFKVFGNDLT
VTMAAEAGQLQLNVMEPVIGESLFQSLRILGNAAKTLREKCVVGITANADVCRAYVDNSIGIITYLNPFLGHDIGDQIGK
EAAETGRPVRELILEKKLMDEKTLEAVLSKENLMHPMFRGRLYLEN
>Mature_525_residues
SKTSNKSSADSKNDAKAEDIVNGENQIATNESQSSDSAAVSERVVEPKTTVQKKFRIESDLLGELQIPSHAYYGVHTLRA
VDNFQISRTTINHVPDFIRGMVQVKKAAALANRRLHTLPAQKAEAIVWACDQILIEERCMDQFPIDVFQGGAGTSLNMNT
NEVVANLALEFLGHEKGEYHILHPMDDVNMSQSTNDSYPTGFRLGIYAGLQTLIAEIDELQVAFRHKGNEFVDIIKMGRT
QLQDAVPMSLGEEFRAFAHNLAEEQTVLREAANRLLEVNLGATAIGTGVNTPAGYRHQVVAALSEVTGLELKSARDLIEA
TSDTGAYVHAHSAIKRAAMKLSKICNDLRLLSSGPRAGLNEINLPPRQAGSSIMPAKVNPVIPEVVNQVCFKVFGNDLTV
TMAAEAGQLQLNVMEPVIGESLFQSLRILGNAAKTLREKCVVGITANADVCRAYVDNSIGIITYLNPFLGHDIGDQIGKE
AAETGRPVRELILEKKLMDEKTLEAVLSKENLMHPMFRGRLYLEN

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily

Homologues:

Organism=Homo sapiens, GI19743875, Length=465, Percent_Identity=37.8494623655914, Blast_Score=318, Evalue=7e-87,
Organism=Escherichia coli, GI87082375, Length=468, Percent_Identity=64.3162393162393, Blast_Score=613, Evalue=1e-177,
Organism=Escherichia coli, GI1787896, Length=470, Percent_Identity=36.3829787234043, Blast_Score=300, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI17553882, Length=470, Percent_Identity=38.0851063829787, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=37.3088685015291, Blast_Score=233, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6324993, Length=462, Percent_Identity=36.5800865800866, Blast_Score=308, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24640179, Length=464, Percent_Identity=37.9310344827586, Blast_Score=328, Evalue=7e-90,
Organism=Drosophila melanogaster, GI24640177, Length=464, Percent_Identity=37.9310344827586, Blast_Score=328, Evalue=8e-90,
Organism=Drosophila melanogaster, GI78710009, Length=461, Percent_Identity=37.7440347071584, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI24662684, Length=470, Percent_Identity=35.7446808510638, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24583245, Length=456, Percent_Identity=34.8684210526316, Blast_Score=280, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASPA_CORGL (Q59200)

Other databases:

- EMBL:   D25316
- EMBL:   BA000036
- EMBL:   BX927152
- PIR:   JC4101
- RefSeq:   NP_600719.1
- RefSeq:   YP_225787.1
- ProteinModelPortal:   Q59200
- SMR:   Q59200
- GeneID:   1019476
- GeneID:   3343809
- GenomeReviews:   BA000036_GR
- GenomeReviews:   BX927147_GR
- KEGG:   cgb:cg1697
- KEGG:   cgl:NCgl1446
- HOGENOM:   HBG284369
- OMA:   VGKICAQ
- PhylomeDB:   Q59200
- ProtClustDB:   PRK12273
- BioCyc:   CGLU196627:CG1697-MONOMER
- BRENDA:   4.3.1.1
- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- PRINTS:   PR00145
- PRINTS:   PR00149
- TIGRFAMs:   TIGR00839

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1; SSF48557 L-Aspartase-like

EC number: =4.3.1.1

Molecular weight: Translated: 57569; Mature: 57438

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: BINDING 155-155

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTSNKSSADSKNDAKAEDIVNGENQIATNESQSSDSAAVSERVVEPKTTVQKKFRIES
CCCCCCCCCCCCCCCCHHHHHCCCCCCEECCCCCCCCHHHHHHHHCCCHHHHHHHHHCCH
DLLGELQIPSHAYYGVHTLRAVDNFQISRTTINHVPDFIRGMVQVKKAAALANRRLHTLP
HHHCCCCCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCC
AQKAEAIVWACDQILIEERCMDQFPIDVFQGGAGTSLNMNTNEVVANLALEFLGHEKGEY
CHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCE
HILHPMDDVNMSQSTNDSYPTGFRLGIYAGLQTLIAEIDELQVAFRHKGNEFVDIIKMGR
EEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TQLQDAVPMSLGEEFRAFAHNLAEEQTVLREAANRLLEVNLGATAIGTGVNTPAGYRHQV
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEECCCCCCCCCHHHHH
VAALSEVTGLELKSARDLIEATSDTGAYVHAHSAIKRAAMKLSKICNDLRLLSSGPRAGL
HHHHHHHHCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
NEINLPPRQAGSSIMPAKVNPVIPEVVNQVCFKVFGNDLTVTMAAEAGQLQLNVMEPVIG
CCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEHHHHHHH
ESLFQSLRILGNAAKTLREKCVVGITANADVCRAYVDNSIGIITYLNPFLGHDIGDQIGK
HHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHCCCCEEEEHHCHHHCCCHHHHHHH
EAAETGRPVRELILEKKLMDEKTLEAVLSKENLMHPMFRGRLYLEN
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECC
>Mature Secondary Structure 
SKTSNKSSADSKNDAKAEDIVNGENQIATNESQSSDSAAVSERVVEPKTTVQKKFRIES
CCCCCCCCCCCCCCCHHHHHCCCCCCEECCCCCCCCHHHHHHHHCCCHHHHHHHHHCCH
DLLGELQIPSHAYYGVHTLRAVDNFQISRTTINHVPDFIRGMVQVKKAAALANRRLHTLP
HHHCCCCCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCC
AQKAEAIVWACDQILIEERCMDQFPIDVFQGGAGTSLNMNTNEVVANLALEFLGHEKGEY
CHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCE
HILHPMDDVNMSQSTNDSYPTGFRLGIYAGLQTLIAEIDELQVAFRHKGNEFVDIIKMGR
EEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TQLQDAVPMSLGEEFRAFAHNLAEEQTVLREAANRLLEVNLGATAIGTGVNTPAGYRHQV
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEECCCCCCCCCHHHHH
VAALSEVTGLELKSARDLIEATSDTGAYVHAHSAIKRAAMKLSKICNDLRLLSSGPRAGL
HHHHHHHHCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
NEINLPPRQAGSSIMPAKVNPVIPEVVNQVCFKVFGNDLTVTMAAEAGQLQLNVMEPVIG
CCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEHHHHHHH
ESLFQSLRILGNAAKTLREKCVVGITANADVCRAYVDNSIGIITYLNPFLGHDIGDQIGK
HHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHCCCCEEEEHHCHHHCCCHHHHHHH
EAAETGRPVRELILEKKLMDEKTLEAVLSKENLMHPMFRGRLYLEN
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7789816; 12948626