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The map label for this gene is fadD4 [H]

Identifier: 62390086

GI number: 62390086

Start: 1264351

End: 1266078

Strand: Reverse

Name: fadD4 [H]

Synonym: cg1353

Alternate gene names: 62390086

Gene position: 1266078-1264351 (Counterclockwise)

Preceding gene: 62390105

Following gene: 62390085

Centisome position: 38.57

GC content: 55.5

Gene sequence:

>1728_bases
ATGCTCAGCACGATGCAGGACGTCCCACTGTCATTAACCAGAATCCTCGAGTACGGGTCCACTGTTCACGGTGATACTTT
GATCACCACGTGGGGCGGTGCCGATGGCATTGAACAAGCACAGCAAACTTTTAGTGCTGTGGGGGCTAGAGCTGCGGCTT
TGGCTCATGCGCTGCATGATTCTTTAGGTATTACTGGGGATCAGCGAGTGGCGTCGATGCTCTATAACTGCGCGGAACAC
ATGGAAACTATGTTTGCAGTCGCATGCATGGGTGCCGTGTTTAATCCGCTGAACAAGCAGTTGATGAATGATCAGATTGT
GTTCATTCTCAATCACTCTGAAGCTGAAGTGGTTATCGCTGATCCGCGCATGGCTGAACAATTGGGTGAGATCTTAAAAG
AAACACCAAAAGTTCGTGCCGTGGTGTTTATTGGACCGAATGATTTCTCTAGCGCGGCGGCCCACATGCCGGAGGGAATG
AAGCTGTATTCCTATGAAGCGCTCCTTGACGGCCGTTCCACTGTTTACAACTGGCCCGAGCAGGATGAACGCACTGCTGC
TGCAATTTGCTATTCCACCGGTACATCGGGACCGCCGAAGGGTGTGGTGTATTCCCACCGCTCGCTTTATTTACAGTCGT
TGAGCTTGCGCACCACGGATTCCCTCGCAGTGGAACACGGCGAAACGTTCCTGTGCTGTGTGCCGATTTACCACGTCCTC
AGCTGGGGCGTGCCGATCGCAGCGTTTATGTCCGGCACTCCCCTGGTGCTTCCTGGACCGGATCTTTCTGCGCCGACATT
GGCAAAGATCATTTCCACCACGCTGCCTCGCGTGGCGCATGGCGTGCCTACTCTCTGGATTCAGCTGATGGTTCACTACC
TGAAAAATCCCCCAGAGCGTATGTCTCTGCGTGAGCTATACGTGGGCGGTTCTGCGGTGCCTCCAATCGTGATCACCATG
TGGGAGCAGCGCTATGGCGTGGATGTTGTCCACGTGTGGGGTATGACGGAAACCTCCACCGTGGGTACTGTGTCTCGCCC
ACCATCAGGTGTTTCTGGTGAAAGCCGGTGGAATTATCGAGTCTCCCAGGGCCGATTCCCCGCCTCCCTGCAGTACCGCA
TTGTCAACGACGGCCAGGTCATGGCGTCCACCGACCGCAACGAGGGCGAGATTCAGGTCCGCGGTCCGTGGGTGACTGCA
AGTTACTTCCACCCCGATGTGGAAAAAGAAGGTGGCACCGCCTCAACATTCCGCGACCATGACGTTGAAGAGGAAAACGA
TGAGCTCTTCACCGCCGACGGCTGGCTGCGCACCGGGGACGTTGGATCTGTCACCAGCGATGGATTCCTCACCATCCAAG
ACCGCGCCCGCGATGTCATCCGTTCTGGCGGCGAGTGGATTTACTCCGCTCAGCTGGAAAACCTCATCGTGGCTACGGAA
GAGGTTGTCGAATGCGCCGTCATTGGCTTCCCCGATGACAAGTGGGTGGAACGTCCCCTCGCAGTCACCATGCTCTACCC
CGGCATTGAACGCACCCGGGAAACCGCCGAGCGCCTCCGCGATCAACTTCGCGACCGCCTACCCAACTGGATGCTGCCAG
AATATTGGACATTTGTTGATGAGGTGGATAAAACCTCCGTCGGTAAATATGACAAGAAGGACCTCCGCAACCACCTGCGC
AACGGCGATTTCGAAGTAATTAAGCTTAAAGGGCCAGGTGAAAAATAA

Upstream 100 bases:

>100_bases
CTCATTCCCCTCGCTAAAAGCTGCATAAAGTTTCGACGTTTTCAAAGTTGATTGCTTGCACTGTCGTTGCGTGTCGCATG
CCCCGGCTATTGTTGATTGC

Downstream 100 bases:

>100_bases
CTTCCCTATTTATTCCGGCTTTTACTGGCTTTATTCCGCAGCTCTTTCTAGGATGGGCAAGAAGATGACTACTCGCCTTT
ATCTTTCGCCAACGCCGCCC

Product: long-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MLSTMQDVPLSLTRILEYGSTVHGDTLITTWGGADGIEQAQQTFSAVGARAAALAHALHDSLGITGDQRVASMLYNCAEH
METMFAVACMGAVFNPLNKQLMNDQIVFILNHSEAEVVIADPRMAEQLGEILKETPKVRAVVFIGPNDFSSAAAHMPEGM
KLYSYEALLDGRSTVYNWPEQDERTAAAICYSTGTSGPPKGVVYSHRSLYLQSLSLRTTDSLAVEHGETFLCCVPIYHVL
SWGVPIAAFMSGTPLVLPGPDLSAPTLAKIISTTLPRVAHGVPTLWIQLMVHYLKNPPERMSLRELYVGGSAVPPIVITM
WEQRYGVDVVHVWGMTETSTVGTVSRPPSGVSGESRWNYRVSQGRFPASLQYRIVNDGQVMASTDRNEGEIQVRGPWVTA
SYFHPDVEKEGGTASTFRDHDVEEENDELFTADGWLRTGDVGSVTSDGFLTIQDRARDVIRSGGEWIYSAQLENLIVATE
EVVECAVIGFPDDKWVERPLAVTMLYPGIERTRETAERLRDQLRDRLPNWMLPEYWTFVDEVDKTSVGKYDKKDLRNHLR
NGDFEVIKLKGPGEK

Sequences:

>Translated_575_residues
MLSTMQDVPLSLTRILEYGSTVHGDTLITTWGGADGIEQAQQTFSAVGARAAALAHALHDSLGITGDQRVASMLYNCAEH
METMFAVACMGAVFNPLNKQLMNDQIVFILNHSEAEVVIADPRMAEQLGEILKETPKVRAVVFIGPNDFSSAAAHMPEGM
KLYSYEALLDGRSTVYNWPEQDERTAAAICYSTGTSGPPKGVVYSHRSLYLQSLSLRTTDSLAVEHGETFLCCVPIYHVL
SWGVPIAAFMSGTPLVLPGPDLSAPTLAKIISTTLPRVAHGVPTLWIQLMVHYLKNPPERMSLRELYVGGSAVPPIVITM
WEQRYGVDVVHVWGMTETSTVGTVSRPPSGVSGESRWNYRVSQGRFPASLQYRIVNDGQVMASTDRNEGEIQVRGPWVTA
SYFHPDVEKEGGTASTFRDHDVEEENDELFTADGWLRTGDVGSVTSDGFLTIQDRARDVIRSGGEWIYSAQLENLIVATE
EVVECAVIGFPDDKWVERPLAVTMLYPGIERTRETAERLRDQLRDRLPNWMLPEYWTFVDEVDKTSVGKYDKKDLRNHLR
NGDFEVIKLKGPGEK
>Mature_575_residues
MLSTMQDVPLSLTRILEYGSTVHGDTLITTWGGADGIEQAQQTFSAVGARAAALAHALHDSLGITGDQRVASMLYNCAEH
METMFAVACMGAVFNPLNKQLMNDQIVFILNHSEAEVVIADPRMAEQLGEILKETPKVRAVVFIGPNDFSSAAAHMPEGM
KLYSYEALLDGRSTVYNWPEQDERTAAAICYSTGTSGPPKGVVYSHRSLYLQSLSLRTTDSLAVEHGETFLCCVPIYHVL
SWGVPIAAFMSGTPLVLPGPDLSAPTLAKIISTTLPRVAHGVPTLWIQLMVHYLKNPPERMSLRELYVGGSAVPPIVITM
WEQRYGVDVVHVWGMTETSTVGTVSRPPSGVSGESRWNYRVSQGRFPASLQYRIVNDGQVMASTDRNEGEIQVRGPWVTA
SYFHPDVEKEGGTASTFRDHDVEEENDELFTADGWLRTGDVGSVTSDGFLTIQDRARDVIRSGGEWIYSAQLENLIVATE
EVVECAVIGFPDDKWVERPLAVTMLYPGIERTRETAERLRDQLRDRLPNWMLPEYWTFVDEVDKTSVGKYDKKDLRNHLR
NGDFEVIKLKGPGEK

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=562, Percent_Identity=25.4448398576512, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI187761343, Length=562, Percent_Identity=25.4448398576512, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI156151445, Length=375, Percent_Identity=26.1333333333333, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI157311624, Length=525, Percent_Identity=22.8571428571429, Blast_Score=106, Evalue=7e-23,
Organism=Homo sapiens, GI157311622, Length=525, Percent_Identity=22.8571428571429, Blast_Score=106, Evalue=7e-23,
Organism=Homo sapiens, GI58082049, Length=525, Percent_Identity=22.2857142857143, Blast_Score=100, Evalue=7e-21,
Organism=Homo sapiens, GI122937307, Length=542, Percent_Identity=24.7232472324723, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI38505220, Length=538, Percent_Identity=24.1635687732342, Blast_Score=93, Evalue=7e-19,
Organism=Homo sapiens, GI115511026, Length=550, Percent_Identity=22, Blast_Score=86, Evalue=8e-17,
Organism=Escherichia coli, GI1788107, Length=547, Percent_Identity=25.0457038391225, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI221142682, Length=512, Percent_Identity=23.6328125, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI145693145, Length=537, Percent_Identity=23.8361266294227, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1786810, Length=380, Percent_Identity=25.7894736842105, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1789201, Length=321, Percent_Identity=23.6760124610592, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1790505, Length=559, Percent_Identity=22.8980322003578, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17558820, Length=521, Percent_Identity=26.295585412668, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17559526, Length=543, Percent_Identity=25.5985267034991, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI32563687, Length=523, Percent_Identity=21.6061185468451, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71994694, Length=551, Percent_Identity=23.5934664246824, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71994703, Length=551, Percent_Identity=23.5934664246824, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI71994690, Length=551, Percent_Identity=23.5934664246824, Blast_Score=105, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17531443, Length=484, Percent_Identity=22.3140495867769, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17560308, Length=502, Percent_Identity=23.1075697211155, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17560140, Length=494, Percent_Identity=21.2550607287449, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17538037, Length=534, Percent_Identity=21.7228464419476, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17557194, Length=541, Percent_Identity=22.181146025878, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71985884, Length=370, Percent_Identity=22.7027027027027, Blast_Score=78, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=492, Percent_Identity=24.7967479674797, Blast_Score=94, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6319264, Length=555, Percent_Identity=23.2432432432432, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24581924, Length=603, Percent_Identity=24.7097844112769, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI21355181, Length=532, Percent_Identity=22.9323308270677, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI18859661, Length=534, Percent_Identity=22.4719101123595, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19922652, Length=540, Percent_Identity=22.2222222222222, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 63716; Mature: 63716

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSTMQDVPLSLTRILEYGSTVHGDTLITTWGGADGIEQAQQTFSAVGARAAALAHALHD
CCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SLGITGDQRVASMLYNCAEHMETMFAVACMGAVFNPLNKQLMNDQIVFILNHSEAEVVIA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEE
DPRMAEQLGEILKETPKVRAVVFIGPNDFSSAAAHMPEGMKLYSYEALLDGRSTVYNWPE
CCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHCCCCCCEEEEEHHHHCCCHHCCCCCC
QDERTAAAICYSTGTSGPPKGVVYSHRSLYLQSLSLRTTDSLAVEHGETFLCCVPIYHVL
CCCCCEEEEEEECCCCCCCCCEEECHHHHHHHHHCCCCCCHHEEECCCEEEEHHHHHHHH
SWGVPIAAFMSGTPLVLPGPDLSAPTLAKIISTTLPRVAHGVPTLWIQLMVHYLKNPPER
HCCCCHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHH
MSLRELYVGGSAVPPIVITMWEQRYGVDVVHVWGMTETSTVGTVSRPPSGVSGESRWNYR
HHHHHHHCCCCCCCCEEEEEEHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEE
VSQGRFPASLQYRIVNDGQVMASTDRNEGEIQVRGPWVTASYFHPDVEKEGGTASTFRDH
ECCCCCCCEEEEEEECCCEEEEECCCCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCCC
DVEEENDELFTADGWLRTGDVGSVTSDGFLTIQDRARDVIRSGGEWIYSAQLENLIVATE
CCCCCCCCEEEECCCEEECCCCCCCCCCEEEEHHHHHHHHHCCCCEEEEEHHHHHHHHHH
EVVECAVIGFPDDKWVERPLAVTMLYPGIERTRETAERLRDQLRDRLPNWMLPEYWTFVD
HHHEEEEECCCCHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
EVDKTSVGKYDKKDLRNHLRNGDFEVIKLKGPGEK
HHCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCC
>Mature Secondary Structure
MLSTMQDVPLSLTRILEYGSTVHGDTLITTWGGADGIEQAQQTFSAVGARAAALAHALHD
CCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SLGITGDQRVASMLYNCAEHMETMFAVACMGAVFNPLNKQLMNDQIVFILNHSEAEVVIA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEE
DPRMAEQLGEILKETPKVRAVVFIGPNDFSSAAAHMPEGMKLYSYEALLDGRSTVYNWPE
CCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHCCCCCCEEEEEHHHHCCCHHCCCCCC
QDERTAAAICYSTGTSGPPKGVVYSHRSLYLQSLSLRTTDSLAVEHGETFLCCVPIYHVL
CCCCCEEEEEEECCCCCCCCCEEECHHHHHHHHHCCCCCCHHEEECCCEEEEHHHHHHHH
SWGVPIAAFMSGTPLVLPGPDLSAPTLAKIISTTLPRVAHGVPTLWIQLMVHYLKNPPER
HCCCCHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHH
MSLRELYVGGSAVPPIVITMWEQRYGVDVVHVWGMTETSTVGTVSRPPSGVSGESRWNYR
HHHHHHHCCCCCCCCEEEEEEHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEE
VSQGRFPASLQYRIVNDGQVMASTDRNEGEIQVRGPWVTASYFHPDVEKEGGTASTFRDH
ECCCCCCCEEEEEEECCCEEEEECCCCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCCC
DVEEENDELFTADGWLRTGDVGSVTSDGFLTIQDRARDVIRSGGEWIYSAQLENLIVATE
CCCCCCCCEEEECCCEEECCCCCCCCCCEEEEHHHHHHHHHCCCCEEEEEHHHHHHHHHH
EVVECAVIGFPDDKWVERPLAVTMLYPGIERTRETAERLRDQLRDRLPNWMLPEYWTFVD
HHHEEEEECCCCHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
EVDKTSVGKYDKKDLRNHLRNGDFEVIKLKGPGEK
HHCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]