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The map label for this gene is metE [H]

Identifier: 62390029

GI number: 62390029

Start: 1189853

End: 1192090

Strand: Reverse

Name: metE [H]

Synonym: cg1290

Alternate gene names: 62390029

Gene position: 1192090-1189853 (Counterclockwise)

Preceding gene: 62390034

Following gene: 62390026

Centisome position: 36.31

GC content: 57.06

Gene sequence:

>2238_bases
ATGACTTCCAACTTTTCTTCCACTGTCGCTGGTCTTCCTCGCATCGGAGCGAAGCGTGAACTGAAGTTCGCGCTCGAAGG
CTACTGGAATGGATCAATTGAAGGTCGCGAACTTGCGCAGACCGCCCGCCAATTGGTCAACACTGCATCGGATTCTTTGT
CTGGATTGGATTCCGTTCCGTTTGCAGGACGTTCCTACTACGACGCAATGCTCGATACCGCCGCTATTTTGGGTGTGCTG
CCGGAGCGTTTTGATGACATCGCTGATCATGAAAACGATGGTCTCCCACTGTGGATTGACCGCTACTTTGGCGCTGCTCG
CGGTACTGAGACCCTGCCTGCACAGGCAATGACCAAGTGGTTTGATACCAACTACCACTACCTCGTGCCGGAGTTGTCTG
CGGATACACGTTTCGTTTTGGATGCGTCCGCGCTGATTGAGGATCTCCGTTGCCAGCAGGTTCGTGGCGTTAATGCCCGC
CCTGTTCTGGTTGGTCCACTGACTTTCCTTTCCCTTGCTCGCACCACTGATGGTTCCAATCCTTTGGATCACCTGCCTGC
ACTGTTTGAGGTCTACGAGCGCCTCATCAAGTCTTTCGATACTGAGTGGGTTCAGATCGATGAGCCTGCGTTGGTCACCG
ATGTTGCTCCTGAGGTTTTGGAGCAGGTCCGCGCTGGTTACACCACTTTGGCTAAGCGCGATGGCGTGTTTGTCAATACT
TACTTCGGCTCTGGCGATCAGGCGCTGAACACTCTTGCGGGCATCGGCCTTGGCGCGATTGGCGTTGACTTGGTCACCCA
TGGCGTCACTGAGCTTGCTGCGTGGAAGGGTGAGGAGCTGCTGGTTGCGGGCATCGTTGATGGTCGTAACATTTGGCGCA
CCGACCTGTGTGCTGCTCTTGCTTCCCTGAAGCGCCTGGCAGCTCGCGGCCCAATCGCAGTGTCTACCTCTTGTTCACTG
CTGCACGTTCCTTACACCCTCGAGGCTGAGAACATTGAGCCTGAGGTCCGCGACTGGCTTGCCTTCGGCTCGGAGAAGAT
CACCGAGGTCAAGCTGCTTGCCGACGCCCTAGCCGGCAACATCGACGCGGCTGCGTTCGATGCGGCGTCCGCAGCAATTG
CTTCTCGACGCACCTCCCCACGCACCGCACCAATCACGCAGGAACTCCCTGGCCGTAGCCGTGGATCCTTCGACACTCGT
GTTACGCTGCAGGAGAAGTCACTGGAGCTTCCAGCTCTGCCAACCACCACCATTGGTTCTTTCCCACAGACCCCATCCAT
TCGTTCTGCTCGCGCTCGTCTGCGCAAGGAATCCATCACTTTGGAGCAGTACGAAGAGGCAATGCGCGAAGAAATCGATC
TGGTCATCGCCAAGCAGGAAGAACTTGGTCTTGATGTGTTGGTTCACGGTGAGCCAGAGCGCAACGACATGGTTCAGTAC
TTCTCTGAACTTCTCGACGGTTTCCTCTCAACCGCCAACGGCTGGGTCCAAAGCTACGGCTCCCGCTGTGTTCGTCCTCC
AGTGTTGTTCGGAAACGTTTCCCGCCCAGCGCCAATGACTGTCAAGTGGTTCCAGTACGCACAGAGCCTGACCCAGAAGC
ATGTCAAGGGAATGCTCACCGGTCCAGTCACCATCCTTGCATGGTCCTTCGTTCGCGATGATCAGCCGCTGGCTACCACT
GCTGACCAGGTTGCACTGGCACTGCGCGATGAAATTAACGATCTCATCGAGGCTGGCGCGAAGATCATCCAGGTGGATGA
GCCTGCGATTCGTGAACTGTTGCCGCTACGAGACGTCGATAAGCCTGCCTACCTGCAGTGGTCCGTGGACTCCTTCCGCC
TGGCGACTGCCGGCGCACCCGACGACGTCCAAATCCACACCCACATGTGCTACTCCGAGTTCAACGAAGTGATCTCCTCG
GTCATCGCGTTGGATGCCGATGTCACCACCATCGAAGCAGCACGTTCCGACATGCAGGTCCTCGCTGCTCTGAAATCTTC
CGGCTTCGAGCTCGGCGTCGGACCTGGTGTGTGGGATATCCACTCCCCGCGCGTTCCTTCCGCGCAGGAAGTGGACGGTC
TCCTCGAGGCTGCACTGCAGTCCGTGGATCCTCGCCAGCTGTGGGTCAACCCAGACTGTGGTCTGAAGACCCGTGGATGG
CCAGAAGTGGAAGCTTCCCTAAAGGTTCTCGTTGAGTCCGCTAAGCAGGCTCGTGAGAAAATCGGAGCAACTATCTAA

Upstream 100 bases:

>100_bases
CACCCGGTGATTTCGCGAACCTTGAAACATCGTCAGAAGATTGCCGTGCGTCCTAGCCGGGATCCGCACGTTCGGCTCAA
GCAGAAAGTCTTTAACTCAC

Downstream 100 bases:

>100_bases
ATTGGGTTACCGCTAGGAACCCAAAGATTAAGGGCACGAGTGTCACCAGGATTGCCGCACCCATGGCAACACCGAAGGAC
ACCGTGCCCACTCCTATTTG

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 745; Mature: 744

Protein sequence:

>745_residues
MTSNFSSTVAGLPRIGAKRELKFALEGYWNGSIEGRELAQTARQLVNTASDSLSGLDSVPFAGRSYYDAMLDTAAILGVL
PERFDDIADHENDGLPLWIDRYFGAARGTETLPAQAMTKWFDTNYHYLVPELSADTRFVLDASALIEDLRCQQVRGVNAR
PVLVGPLTFLSLARTTDGSNPLDHLPALFEVYERLIKSFDTEWVQIDEPALVTDVAPEVLEQVRAGYTTLAKRDGVFVNT
YFGSGDQALNTLAGIGLGAIGVDLVTHGVTELAAWKGEELLVAGIVDGRNIWRTDLCAALASLKRLAARGPIAVSTSCSL
LHVPYTLEAENIEPEVRDWLAFGSEKITEVKLLADALAGNIDAAAFDAASAAIASRRTSPRTAPITQELPGRSRGSFDTR
VTLQEKSLELPALPTTTIGSFPQTPSIRSARARLRKESITLEQYEEAMREEIDLVIAKQEELGLDVLVHGEPERNDMVQY
FSELLDGFLSTANGWVQSYGSRCVRPPVLFGNVSRPAPMTVKWFQYAQSLTQKHVKGMLTGPVTILAWSFVRDDQPLATT
ADQVALALRDEINDLIEAGAKIIQVDEPAIRELLPLRDVDKPAYLQWSVDSFRLATAGAPDDVQIHTHMCYSEFNEVISS
VIALDADVTTIEAARSDMQVLAALKSSGFELGVGPGVWDIHSPRVPSAQEVDGLLEAALQSVDPRQLWVNPDCGLKTRGW
PEVEASLKVLVESAKQAREKIGATI

Sequences:

>Translated_745_residues
MTSNFSSTVAGLPRIGAKRELKFALEGYWNGSIEGRELAQTARQLVNTASDSLSGLDSVPFAGRSYYDAMLDTAAILGVL
PERFDDIADHENDGLPLWIDRYFGAARGTETLPAQAMTKWFDTNYHYLVPELSADTRFVLDASALIEDLRCQQVRGVNAR
PVLVGPLTFLSLARTTDGSNPLDHLPALFEVYERLIKSFDTEWVQIDEPALVTDVAPEVLEQVRAGYTTLAKRDGVFVNT
YFGSGDQALNTLAGIGLGAIGVDLVTHGVTELAAWKGEELLVAGIVDGRNIWRTDLCAALASLKRLAARGPIAVSTSCSL
LHVPYTLEAENIEPEVRDWLAFGSEKITEVKLLADALAGNIDAAAFDAASAAIASRRTSPRTAPITQELPGRSRGSFDTR
VTLQEKSLELPALPTTTIGSFPQTPSIRSARARLRKESITLEQYEEAMREEIDLVIAKQEELGLDVLVHGEPERNDMVQY
FSELLDGFLSTANGWVQSYGSRCVRPPVLFGNVSRPAPMTVKWFQYAQSLTQKHVKGMLTGPVTILAWSFVRDDQPLATT
ADQVALALRDEINDLIEAGAKIIQVDEPAIRELLPLRDVDKPAYLQWSVDSFRLATAGAPDDVQIHTHMCYSEFNEVISS
VIALDADVTTIEAARSDMQVLAALKSSGFELGVGPGVWDIHSPRVPSAQEVDGLLEAALQSVDPRQLWVNPDCGLKTRGW
PEVEASLKVLVESAKQAREKIGATI
>Mature_744_residues
TSNFSSTVAGLPRIGAKRELKFALEGYWNGSIEGRELAQTARQLVNTASDSLSGLDSVPFAGRSYYDAMLDTAAILGVLP
ERFDDIADHENDGLPLWIDRYFGAARGTETLPAQAMTKWFDTNYHYLVPELSADTRFVLDASALIEDLRCQQVRGVNARP
VLVGPLTFLSLARTTDGSNPLDHLPALFEVYERLIKSFDTEWVQIDEPALVTDVAPEVLEQVRAGYTTLAKRDGVFVNTY
FGSGDQALNTLAGIGLGAIGVDLVTHGVTELAAWKGEELLVAGIVDGRNIWRTDLCAALASLKRLAARGPIAVSTSCSLL
HVPYTLEAENIEPEVRDWLAFGSEKITEVKLLADALAGNIDAAAFDAASAAIASRRTSPRTAPITQELPGRSRGSFDTRV
TLQEKSLELPALPTTTIGSFPQTPSIRSARARLRKESITLEQYEEAMREEIDLVIAKQEELGLDVLVHGEPERNDMVQYF
SELLDGFLSTANGWVQSYGSRCVRPPVLFGNVSRPAPMTVKWFQYAQSLTQKHVKGMLTGPVTILAWSFVRDDQPLATTA
DQVALALRDEINDLIEAGAKIIQVDEPAIRELLPLRDVDKPAYLQWSVDSFRLATAGAPDDVQIHTHMCYSEFNEVISSV
IALDADVTTIEAARSDMQVLAALKSSGFELGVGPGVWDIHSPRVPSAQEVDGLLEAALQSVDPRQLWVNPDCGLKTRGWP
EVEASLKVLVESAKQAREKIGATI

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=752, Percent_Identity=45.3457446808511, Blast_Score=621, Evalue=1e-179,
Organism=Saccharomyces cerevisiae, GI6320936, Length=777, Percent_Identity=42.2136422136422, Blast_Score=562, Evalue=1e-161,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 81314; Mature: 81183

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSNFSSTVAGLPRIGAKRELKFALEGYWNGSIEGRELAQTARQLVNTASDSLSGLDSVP
CCCCCCHHHHCCCCCCCCHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
FAGRSYYDAMLDTAAILGVLPERFDDIADHENDGLPLWIDRYFGAARGTETLPAQAMTKW
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHCCCCCCCCCCHHHHHHH
FDTNYHYLVPELSADTRFVLDASALIEDLRCQQVRGVNARPVLVGPLTFLSLARTTDGSN
HCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHCCCCCC
PLDHLPALFEVYERLIKSFDTEWVQIDEPALVTDVAPEVLEQVRAGYTTLAKRDGVFVNT
CHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCHHHHHHCCCEEEEE
YFGSGDQALNTLAGIGLGAIGVDLVTHGVTELAAWKGEELLVAGIVDGRNIWRTDLCAAL
EECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHH
ASLKRLAARGPIAVSTSCSLLHVPYTLEAENIEPEVRDWLAFGSEKITEVKLLADALAGN
HHHHHHHHCCCEEEECCCCEEECCEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCC
IDAAAFDAASAAIASRRTSPRTAPITQELPGRSRGSFDTRVTLQEKSLELPALPTTTIGS
CCHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
FPQTPSIRSARARLRKESITLEQYEEAMREEIDLVIAKQEELGLDVLVHGEPERNDMVQY
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEECHHHCCCEEEEECCCCHHHHHHH
FSELLDGFLSTANGWVQSYGSRCVRPPVLFGNVSRPAPMTVKWFQYAQSLTQKHVKGMLT
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GPVTILAWSFVRDDQPLATTADQVALALRDEINDLIEAGAKIIQVDEPAIRELLPLRDVD
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHCCCCCCC
KPAYLQWSVDSFRLATAGAPDDVQIHTHMCYSEFNEVISSVIALDADVTTIEAARSDMQV
CCCEEEECCCCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LAALKSSGFELGVGPGVWDIHSPRVPSAQEVDGLLEAALQSVDPRQLWVNPDCGLKTRGW
HHHHHHCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
PEVEASLKVLVESAKQAREKIGATI
CHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TSNFSSTVAGLPRIGAKRELKFALEGYWNGSIEGRELAQTARQLVNTASDSLSGLDSVP
CCCCCHHHHCCCCCCCCHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
FAGRSYYDAMLDTAAILGVLPERFDDIADHENDGLPLWIDRYFGAARGTETLPAQAMTKW
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHCCCCCCCCCCHHHHHHH
FDTNYHYLVPELSADTRFVLDASALIEDLRCQQVRGVNARPVLVGPLTFLSLARTTDGSN
HCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHCCCCCC
PLDHLPALFEVYERLIKSFDTEWVQIDEPALVTDVAPEVLEQVRAGYTTLAKRDGVFVNT
CHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCHHHHHHCCCEEEEE
YFGSGDQALNTLAGIGLGAIGVDLVTHGVTELAAWKGEELLVAGIVDGRNIWRTDLCAAL
EECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHH
ASLKRLAARGPIAVSTSCSLLHVPYTLEAENIEPEVRDWLAFGSEKITEVKLLADALAGN
HHHHHHHHCCCEEEECCCCEEECCEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCC
IDAAAFDAASAAIASRRTSPRTAPITQELPGRSRGSFDTRVTLQEKSLELPALPTTTIGS
CCHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
FPQTPSIRSARARLRKESITLEQYEEAMREEIDLVIAKQEELGLDVLVHGEPERNDMVQY
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEECHHHCCCEEEEECCCCHHHHHHH
FSELLDGFLSTANGWVQSYGSRCVRPPVLFGNVSRPAPMTVKWFQYAQSLTQKHVKGMLT
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GPVTILAWSFVRDDQPLATTADQVALALRDEINDLIEAGAKIIQVDEPAIRELLPLRDVD
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHCCCCCCC
KPAYLQWSVDSFRLATAGAPDDVQIHTHMCYSEFNEVISSVIALDADVTTIEAARSDMQV
CCCEEEECCCCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LAALKSSGFELGVGPGVWDIHSPRVPSAQEVDGLLEAALQSVDPRQLWVNPDCGLKTRGW
HHHHHHCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
PEVEASLKVLVESAKQAREKIGATI
CHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA