Click here to switch to the map view.

The map label for this gene is ynfL [H]

Identifier: 62389354

GI number: 62389354

Start: 478088

End: 478972

Strand: Reverse

Name: ynfL [H]

Synonym: cg0537

Alternate gene names: 62389354

Gene position: 478972-478088 (Counterclockwise)

Preceding gene: 62389355

Following gene: 62389353

Centisome position: 14.59

GC content: 51.53

Gene sequence:

>885_bases
TTGTTGTTCACTCTTGAACAGTTGCGGTGTTTTGTCGCCGTCGCCAATCATCTTCATTTCGGAAAAGCTGCTGCAGAGCT
ATCCATGACGCAGCCGCCGTTGAGTCGTCAGATTCAAAAGCTGGAGAAGATCGTCGGTGCAACCCTGCTTGATCGTGACA
ACCGCAAGGTGGAACTGACCACTGCGGGTTTCGCATTTTTGAAGGATGCTCGCCTCATTCTCAATTCCACCGAGAAGGCG
GCTGAGCGCGCACGATTGGCTAGCTCTGGCATGTGGGGACAGCTCAATATTGGATACACCGCTGCAGCGGGTTTTTCCAT
TCTGGGCCCGACGTTGAATCAGTTGCATGAGAAGATGCCGGGGGTCAGTGTCGATCTTTTTGAGATGGTCTCCACCGAGC
AGATCGCCGCCTTGGAATCTGGGCTACTGGATCTTGGCATTGGCCGATTGAGCTCGCCAGTTGAGGGTCTTCAAACTCGA
CGTCTCCAGGCAGATTCCTTGGTTCTTGCAGCTCCGAAGGGGCATCCACTTCTTGATCAGAATCGACCACTGTTGCGGAA
GCATCTGACTGGGGTTCCTTTTCTGCAGCACTCTCCCACCAAGGCGAAGTACCTCTACGACATCGTTGTTAGAAACTTCA
CGATCAATGATGCGCAGGTGCAACATACGCTGAGCCAGATCACCACGATGGTTAGTCTGGTGGCCTCTGGACTGGGTGTT
GCGCTGGTTCCGGAGTCTGCGAAAAAACTCAATTACAGCGGTGTTGAGTATCGCCATTTTTATGATCTACCTGTTGGTTT
AGCGGAGCTGCAGGCTATTTATTCCACCTCGAATGATAATCCTGCGGTGCGGAAATTCATCAAAAACATTGACGATACCT
TTTAA

Upstream 100 bases:

>100_bases
GCATGTTCACAGTCAGCCACCTGAGGATGAAGACATCGATCGAATTCAGAAAAAGCTGCAGGCTGAGGGCTTCCCCACCC
GCAATTAATTAATTGGAGTT

Downstream 100 bases:

>100_bases
GCATTTCAACATGCCAAACTAATCTTGGACAGGTATCACTGGCTCCTCTTAGACTCCAAAGTGTCTTAGAAAACACTCAC
CCAAGGAGCCCTCATGGCAC

Product: transcriptional regulator LYSR-type

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MLFTLEQLRCFVAVANHLHFGKAAAELSMTQPPLSRQIQKLEKIVGATLLDRDNRKVELTTAGFAFLKDARLILNSTEKA
AERARLASSGMWGQLNIGYTAAAGFSILGPTLNQLHEKMPGVSVDLFEMVSTEQIAALESGLLDLGIGRLSSPVEGLQTR
RLQADSLVLAAPKGHPLLDQNRPLLRKHLTGVPFLQHSPTKAKYLYDIVVRNFTINDAQVQHTLSQITTMVSLVASGLGV
ALVPESAKKLNYSGVEYRHFYDLPVGLAELQAIYSTSNDNPAVRKFIKNIDDTF

Sequences:

>Translated_294_residues
MLFTLEQLRCFVAVANHLHFGKAAAELSMTQPPLSRQIQKLEKIVGATLLDRDNRKVELTTAGFAFLKDARLILNSTEKA
AERARLASSGMWGQLNIGYTAAAGFSILGPTLNQLHEKMPGVSVDLFEMVSTEQIAALESGLLDLGIGRLSSPVEGLQTR
RLQADSLVLAAPKGHPLLDQNRPLLRKHLTGVPFLQHSPTKAKYLYDIVVRNFTINDAQVQHTLSQITTMVSLVASGLGV
ALVPESAKKLNYSGVEYRHFYDLPVGLAELQAIYSTSNDNPAVRKFIKNIDDTF
>Mature_294_residues
MLFTLEQLRCFVAVANHLHFGKAAAELSMTQPPLSRQIQKLEKIVGATLLDRDNRKVELTTAGFAFLKDARLILNSTEKA
AERARLASSGMWGQLNIGYTAAAGFSILGPTLNQLHEKMPGVSVDLFEMVSTEQIAALESGLLDLGIGRLSSPVEGLQTR
RLQADSLVLAAPKGHPLLDQNRPLLRKHLTGVPFLQHSPTKAKYLYDIVVRNFTINDAQVQHTLSQITTMVSLVASGLGV
ALVPESAKKLNYSGVEYRHFYDLPVGLAELQAIYSTSNDNPAVRKFIKNIDDTF

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=295, Percent_Identity=35.9322033898305, Blast_Score=157, Evalue=8e-40,
Organism=Escherichia coli, GI1788887, Length=292, Percent_Identity=34.2465753424658, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI2367136, Length=296, Percent_Identity=31.0810810810811, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1790399, Length=250, Percent_Identity=29.2, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081904, Length=184, Percent_Identity=32.0652173913043, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790208, Length=243, Percent_Identity=29.2181069958848, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787601, Length=143, Percent_Identity=35.6643356643357, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI145693105, Length=259, Percent_Identity=25.0965250965251, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1788297, Length=248, Percent_Identity=27.4193548387097, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI157672245, Length=170, Percent_Identity=32.9411764705882, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789173, Length=243, Percent_Identity=28.8065843621399, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788748, Length=248, Percent_Identity=23.7903225806452, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788626, Length=143, Percent_Identity=34.965034965035, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32250; Mature: 32250

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFTLEQLRCFVAVANHLHFGKAAAELSMTQPPLSRQIQKLEKIVGATLLDRDNRKVELT
CCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEE
TAGFAFLKDARLILNSTEKAAERARLASSGMWGQLNIGYTAAAGFSILGPTLNQLHEKMP
ECHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEECCEEHHCCCHHHCHHHHHHHHHCC
GVSVDLFEMVSTEQIAALESGLLDLGIGRLSSPVEGLQTRRLQADSLVLAAPKGHPLLDQ
CCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHCCCCCEEEECCCCCCCCCC
NRPLLRKHLTGVPFLQHSPTKAKYLYDIVVRNFTINDAQVQHTLSQITTMVSLVASGLGV
CCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCE
ALVPESAKKLNYSGVEYRHFYDLPVGLAELQAIYSTSNDNPAVRKFIKNIDDTF
EEECCHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MLFTLEQLRCFVAVANHLHFGKAAAELSMTQPPLSRQIQKLEKIVGATLLDRDNRKVELT
CCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEE
TAGFAFLKDARLILNSTEKAAERARLASSGMWGQLNIGYTAAAGFSILGPTLNQLHEKMP
ECHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEECCEEHHCCCHHHCHHHHHHHHHCC
GVSVDLFEMVSTEQIAALESGLLDLGIGRLSSPVEGLQTRRLQADSLVLAAPKGHPLLDQ
CCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHCCCCCEEEECCCCCCCCCC
NRPLLRKHLTGVPFLQHSPTKAKYLYDIVVRNFTINDAQVQHTLSQITTMVSLVASGLGV
CCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCE
ALVPESAKKLNYSGVEYRHFYDLPVGLAELQAIYSTSNDNPAVRKFIKNIDDTF
EEECCHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]