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The map label for this gene is mnhD [H]

Identifier: 62389165

GI number: 62389165

Start: 281401

End: 282933

Strand: Reverse

Name: mnhD [H]

Synonym: cg0324

Alternate gene names: 62389165

Gene position: 282933-281401 (Counterclockwise)

Preceding gene: 62389166

Following gene: 62389164

Centisome position: 8.62

GC content: 56.36

Gene sequence:

>1533_bases
ATGGCCATGGATGTTCTCCTTCCTATTTTCGTTGCAGTTCCCCTTGCTGCCTCTGCCATTGCGGTGCTTCTGCCGTGGCG
TCTCATCCGCGATATTTTGCACATCATCGTGCCTTTCGCGGGTATTTTTGCTGGCATCTGGTTGTTTGCACACACCGCTG
AACACGGCCCGATTGCTCACAACGTGGGCCTTTATGTCGGTGGCGTGGCAATCCCCTTTGCTGCCGATACGTTCAGCGCC
ATCATGTTGATCACCACCTCGATCGTTGCGGTGGCTGCCAACTGGTTTGCCACCATCGTCGGTGAAACCCGCGCGCGTTT
CTATCCAGCGCTCACATTGATGCTGATCACGGGCGTCAACGGTGCTCTGCTGACTGCCGATCTGTTCAACTTCTTTGTGT
TCATCGAAGTGATGCTGCTGCCTTCCTATGGTTTGATCGCCATGACCGGAACGTGGGCGCGCCTAGCCTCTGGACGAATC
TTCGTACTAGTCAATCTCTCTGCCTCCACATTGCTGGTTGCAGGTGTGGGAATCGTCTACGGTGTCATAGGCTCAGTCAA
CATCGCAGCTCTGCAAGATGTCGTAGAGGGCAACCCCCTGGTTGCCAGCGCAATGGGCATCGTGGTTATTGCCATCGCGG
TTAAAGCCGGTGTATTCCCAGTGCACACATGGCTGCCACGCACCTATCCTGGTACATCAGCAGCTGTGATGGGGTTGTTC
TCCGGTTTGCACACCAAAGTCGCGGTATACATGCTCTATCGCATTTGGGTCCACATTTTTGACATGGATCCCACGTGGAA
TTGGCTGATTGTCGCATTCATGGTGATATCCATGCTGGTCGGTGGCTTCGCTGGACTTGCTGAAAACTCCATCCGTCGCG
TCCTTGCCTACCAAATGGTCAACGGCATGCCATTTATTCTCATCATGATGGCGTTTACCTCTGACGATCCACAGCGCGCA
CTTGCCGCTGGTCTGTTGTACACCCTGCACCACATGATCACCATCGCCGCATTGGTGCTCACTTCCGGCGCAATCGAAGA
AACCTACGGCACCGGTATGTTGTCCAAGCTGTCTGGCCTTGCACGCCGCGAACCCGTCGTCGCAGCAGTGTTCGCTGCAG
GTGCCTTCTCTGTTGTCGGTTTCCCACCGTTTTCCGGTATGTGGGGCAAAGCGCTCATCCTGCTCGAGATCGCCCGCGTC
GGCAATATTGCAGCATGGATCGCAATCGCCGCCATCATCATCGCCAGCCTCGGCGCACTGCTCTCGATGATCCGCGTGTG
GCGTGAAGTCTTCTGGGGTGGCGCAATGCACCAGCGCGGCGTCTCGCCGCAGCTGCGCATCAGCCCAGCAAAAATCGCCC
CAGCGCTCAGCCTGATCATTTTATCGGTAGGCATGTTCATCTTCGCGGGCCCGCTTATCGACGCGACCCTCACCGCCACC
GACGGCCTCTTGAACACCGATGCATACCAACAGGCTGTGCTCGGTGAAAATGCCATCGGAGTGCCAAGCCCTAGCTACCA
GGGAGGAAACTAA

Upstream 100 bases:

>100_bases
CGACGACACCCGGTCCATCGAACCAGATGACGATCAATCGCCTTTGACTACTAGCGCTCGTTCAGTCACCAACCCAACAG
ATCAGGAGGATAAAGCTTAA

Downstream 100 bases:

>100_bases
TGCTTAACGCCCTGAAATTCATCCCATGGCTGATCGGCCAGATTTTCCTCTCTGGCTTCAGCGTGATCACCGCTGCGGTA
AAAAAGGACACCGGCTTCAA

Product: monovalent cation/H+ antiporter subunit D

Products: H2; oxidized ferredoxin [C]

Alternate protein names: Mrp complex subunit D; Multiple resistance and pH homeostasis protein D [H]

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MAMDVLLPIFVAVPLAASAIAVLLPWRLIRDILHIIVPFAGIFAGIWLFAHTAEHGPIAHNVGLYVGGVAIPFAADTFSA
IMLITTSIVAVAANWFATIVGETRARFYPALTLMLITGVNGALLTADLFNFFVFIEVMLLPSYGLIAMTGTWARLASGRI
FVLVNLSASTLLVAGVGIVYGVIGSVNIAALQDVVEGNPLVASAMGIVVIAIAVKAGVFPVHTWLPRTYPGTSAAVMGLF
SGLHTKVAVYMLYRIWVHIFDMDPTWNWLIVAFMVISMLVGGFAGLAENSIRRVLAYQMVNGMPFILIMMAFTSDDPQRA
LAAGLLYTLHHMITIAALVLTSGAIEETYGTGMLSKLSGLARREPVVAAVFAAGAFSVVGFPPFSGMWGKALILLEIARV
GNIAAWIAIAAIIIASLGALLSMIRVWREVFWGGAMHQRGVSPQLRISPAKIAPALSLIILSVGMFIFAGPLIDATLTAT
DGLLNTDAYQQAVLGENAIGVPSPSYQGGN

Sequences:

>Translated_510_residues
MAMDVLLPIFVAVPLAASAIAVLLPWRLIRDILHIIVPFAGIFAGIWLFAHTAEHGPIAHNVGLYVGGVAIPFAADTFSA
IMLITTSIVAVAANWFATIVGETRARFYPALTLMLITGVNGALLTADLFNFFVFIEVMLLPSYGLIAMTGTWARLASGRI
FVLVNLSASTLLVAGVGIVYGVIGSVNIAALQDVVEGNPLVASAMGIVVIAIAVKAGVFPVHTWLPRTYPGTSAAVMGLF
SGLHTKVAVYMLYRIWVHIFDMDPTWNWLIVAFMVISMLVGGFAGLAENSIRRVLAYQMVNGMPFILIMMAFTSDDPQRA
LAAGLLYTLHHMITIAALVLTSGAIEETYGTGMLSKLSGLARREPVVAAVFAAGAFSVVGFPPFSGMWGKALILLEIARV
GNIAAWIAIAAIIIASLGALLSMIRVWREVFWGGAMHQRGVSPQLRISPAKIAPALSLIILSVGMFIFAGPLIDATLTAT
DGLLNTDAYQQAVLGENAIGVPSPSYQGGN
>Mature_509_residues
AMDVLLPIFVAVPLAASAIAVLLPWRLIRDILHIIVPFAGIFAGIWLFAHTAEHGPIAHNVGLYVGGVAIPFAADTFSAI
MLITTSIVAVAANWFATIVGETRARFYPALTLMLITGVNGALLTADLFNFFVFIEVMLLPSYGLIAMTGTWARLASGRIF
VLVNLSASTLLVAGVGIVYGVIGSVNIAALQDVVEGNPLVASAMGIVVIAIAVKAGVFPVHTWLPRTYPGTSAAVMGLFS
GLHTKVAVYMLYRIWVHIFDMDPTWNWLIVAFMVISMLVGGFAGLAENSIRRVLAYQMVNGMPFILIMMAFTSDDPQRAL
AAGLLYTLHHMITIAALVLTSGAIEETYGTGMLSKLSGLARREPVVAAVFAAGAFSVVGFPPFSGMWGKALILLEIARVG
NIAAWIAIAAIIIASLGALLSMIRVWREVFWGGAMHQRGVSPQLRISPAKIAPALSLIILSVGMFIFAGPLIDATLTATD
GLLNTDAYQQAVLGENAIGVPSPSYQGGN

Specific function: Mrp complex is a Na(+)/H(+) antiporter involved in Na(+) excretion and Na(+) resistance [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Escherichia coli, GI1788827, Length=323, Percent_Identity=27.5541795665635, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788613, Length=376, Percent_Identity=26.8617021276596, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788831, Length=289, Percent_Identity=26.2975778546713, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI2367154, Length=335, Percent_Identity=23.2835820895522, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI145693160, Length=337, Percent_Identity=24.6290801186944, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788829, Length=309, Percent_Identity=21.3592233009709, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 54062; Mature: 53931

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMDVLLPIFVAVPLAASAIAVLLPWRLIRDILHIIVPFAGIFAGIWLFAHTAEHGPIAH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
NVGLYVGGVAIPFAADTFSAIMLITTSIVAVAANWFATIVGETRARFYPALTLMLITGVN
CCCEEECCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GALLTADLFNFFVFIEVMLLPSYGLIAMTGTWARLASGRIFVLVNLSASTLLVAGVGIVY
CHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHCCCCEEEEEECCHHHHHHHHHHHHH
GVIGSVNIAALQDVVEGNPLVASAMGIVVIAIAVKAGVFPVHTWLPRTYPGTSAAVMGLF
HHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEHHHHCCCCCCCCHHHHHHHH
SGLHTKVAVYMLYRIWVHIFDMDPTWNWLIVAFMVISMLVGGFAGLAENSIRRVLAYQMV
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NGMPFILIMMAFTSDDPQRALAAGLLYTLHHMITIAALVLTSGAIEETYGTGMLSKLSGL
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHH
ARREPVVAAVFAAGAFSVVGFPPFSGMWGKALILLEIARVGNIAAWIAIAAIIIASLGAL
HHCCCHHHHHHHHCHHHEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LSMIRVWREVFWGGAMHQRGVSPQLRISPAKIAPALSLIILSVGMFIFAGPLIDATLTAT
HHHHHHHHHHHCCCHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
DGLLNTDAYQQAVLGENAIGVPSPSYQGGN
CCCCCCHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AMDVLLPIFVAVPLAASAIAVLLPWRLIRDILHIIVPFAGIFAGIWLFAHTAEHGPIAH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
NVGLYVGGVAIPFAADTFSAIMLITTSIVAVAANWFATIVGETRARFYPALTLMLITGVN
CCCEEECCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GALLTADLFNFFVFIEVMLLPSYGLIAMTGTWARLASGRIFVLVNLSASTLLVAGVGIVY
CHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHCCCCEEEEEECCHHHHHHHHHHHHH
GVIGSVNIAALQDVVEGNPLVASAMGIVVIAIAVKAGVFPVHTWLPRTYPGTSAAVMGLF
HHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEHHHHCCCCCCCCHHHHHHHH
SGLHTKVAVYMLYRIWVHIFDMDPTWNWLIVAFMVISMLVGGFAGLAENSIRRVLAYQMV
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NGMPFILIMMAFTSDDPQRALAAGLLYTLHHMITIAALVLTSGAIEETYGTGMLSKLSGL
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHH
ARREPVVAAVFAAGAFSVVGFPPFSGMWGKALILLEIARVGNIAAWIAIAAIIIASLGAL
HHCCCHHHHHHHHCHHHEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LSMIRVWREVFWGGAMHQRGVSPQLRISPAKIAPALSLIILSVGMFIFAGPLIDATLTAT
HHHHHHHHHHHCCCHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
DGLLNTDAYQQAVLGENAIGVPSPSYQGGN
CCCCCCHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11356194 [H]