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The map label for this gene is rfbE [H]

Identifier: 62389097

GI number: 62389097

Start: 217139

End: 217930

Strand: Reverse

Name: rfbE [H]

Synonym: cg0248

Alternate gene names: 62389097

Gene position: 217930-217139 (Counterclockwise)

Preceding gene: 62389098

Following gene: 62389096

Centisome position: 6.64

GC content: 60.73

Gene sequence:

>792_bases
ATGGTATCCATCGATACATACAACGCCTGCGTCGACTTCCCCATCTTCGACGCCAAATCCCGCTCCATGAAGAAAGCCTT
CCTCGGCGCAGCCGGCGGAGCAATCGGGCGCAATCAAGACAACGTCGTAGTCGTCGAAGCGCTGAAGAACGTCAACCTGC
ACTTGCGCGAAGGTGACCGGGTCGGACTCGTCGGCCACAACGGCGCCGGCAAATCCACCCTCCTGCGACTCCTCTCCGGC
ATCTACGAACCCACCCGCGGAAGCGCTGACATCCGTGGACGCGTCGCCCCCGTCTTCGACCTCGGCGTCGGCATGGATCC
AGAAATCTCCGGCTACGAAAATATCATCATCCGCGGCCTCTTCCTCGGTCAAACCCGCAAACAGATGAAAGCCAAAATGG
AAGAAATCGCCGACTTCACCGAACTCGGCGAATACCTCTCCATGCCTCTCCGAACCTACTCCACCGGCATGCGCATCCGC
CTAGCCCTCGGCGTGGTCACCTCCATCGAGCCCGAAATTCTGCTTCTTGATGAAGGCATCGGCGCCGTCGACGCCGCCTT
CATGGCCAAAGCCCGCGACCGCCTCCAAGCCCTCGTCGAACGATCCGGCATCCTCGTCTTCGCCTCCCACTCCAACGACT
TCCTCGCCCAACTCTGCAACACCGCACTCTGGGTCGACCACGGACAAATCCGCGAAGCGGGACTAGTTCCAGACGTGGTG
GAAGCCTACGAAGGCAAGGGCGCCGGCGACCACGTCCGCAGACTCCTCACCCGCATGGAAGAAGAAAAGTAG

Upstream 100 bases:

>100_bases
TGGATCGTCCTCGCCTTCACATTCGTCGGCCTTGGCCTTGCTCTCCTCGCGATGAAGCAATGGCGATTCCGCGTCAGCTA
CTGGGTATAAGGAGCACCAC

Downstream 100 bases:

>100_bases
CTCCTGCGTTTCGGGTTTGCGCGGTTGGGCGGTTAGTCGCTGGAGGTGACCACCCTGGCGGCAAGTTGGCCCTGTTCTGC
GCGGGTGCGCCAAACAAGGC

Product: polysaccharide/polyol phosphate ABC transporter ATPase

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MVSIDTYNACVDFPIFDAKSRSMKKAFLGAAGGAIGRNQDNVVVVEALKNVNLHLREGDRVGLVGHNGAGKSTLLRLLSG
IYEPTRGSADIRGRVAPVFDLGVGMDPEISGYENIIIRGLFLGQTRKQMKAKMEEIADFTELGEYLSMPLRTYSTGMRIR
LALGVVTSIEPEILLLDEGIGAVDAAFMAKARDRLQALVERSGILVFASHSNDFLAQLCNTALWVDHGQIREAGLVPDVV
EAYEGKGAGDHVRRLLTRMEEEK

Sequences:

>Translated_263_residues
MVSIDTYNACVDFPIFDAKSRSMKKAFLGAAGGAIGRNQDNVVVVEALKNVNLHLREGDRVGLVGHNGAGKSTLLRLLSG
IYEPTRGSADIRGRVAPVFDLGVGMDPEISGYENIIIRGLFLGQTRKQMKAKMEEIADFTELGEYLSMPLRTYSTGMRIR
LALGVVTSIEPEILLLDEGIGAVDAAFMAKARDRLQALVERSGILVFASHSNDFLAQLCNTALWVDHGQIREAGLVPDVV
EAYEGKGAGDHVRRLLTRMEEEK
>Mature_263_residues
MVSIDTYNACVDFPIFDAKSRSMKKAFLGAAGGAIGRNQDNVVVVEALKNVNLHLREGDRVGLVGHNGAGKSTLLRLLSG
IYEPTRGSADIRGRVAPVFDLGVGMDPEISGYENIIIRGLFLGQTRKQMKAKMEEIADFTELGEYLSMPLRTYSTGMRIR
LALGVVTSIEPEILLLDEGIGAVDAAFMAKARDRLQALVERSGILVFASHSNDFLAQLCNTALWVDHGQIREAGLVPDVV
EAYEGKGAGDHVRRLLTRMEEEK

Specific function: May form an ATP-driven O-antigen export apparatus, in association with RfbD [H]

COG id: COG1134

COG function: function code GM; ABC-type polysaccharide/polyol phosphate transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI27436953, Length=237, Percent_Identity=27.0042194092827, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI153792144, Length=206, Percent_Identity=26.2135922330097, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI27477115, Length=210, Percent_Identity=26.6666666666667, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI116734710, Length=162, Percent_Identity=29.0123456790123, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI6005701, Length=187, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1790467, Length=197, Percent_Identity=29.4416243654822, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787758, Length=208, Percent_Identity=30.2884615384615, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI87081709, Length=204, Percent_Identity=26.9607843137255, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI48995001, Length=214, Percent_Identity=27.5700934579439, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1788761, Length=213, Percent_Identity=27.6995305164319, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787547, Length=217, Percent_Identity=25.8064516129032, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081782, Length=218, Percent_Identity=30.2752293577982, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788225, Length=210, Percent_Identity=24.7619047619048, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789032, Length=226, Percent_Identity=27.4336283185841, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787712, Length=199, Percent_Identity=26.1306532663317, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1787759, Length=237, Percent_Identity=28.6919831223629, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87082267, Length=207, Percent_Identity=27.536231884058, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87081791, Length=184, Percent_Identity=29.3478260869565, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789672, Length=210, Percent_Identity=24.7619047619048, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787029, Length=205, Percent_Identity=24.8780487804878, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787089, Length=209, Percent_Identity=25.3588516746411, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1786654, Length=214, Percent_Identity=30.8411214953271, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788450, Length=232, Percent_Identity=25.4310344827586, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787164, Length=199, Percent_Identity=29.1457286432161, Blast_Score=61, Evalue=6e-11,
Organism=Escherichia coli, GI1786319, Length=199, Percent_Identity=25.1256281407035, Blast_Score=61, Evalue=9e-11,
Organism=Escherichia coli, GI48994943, Length=211, Percent_Identity=24.1706161137441, Blast_Score=60, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI17565586, Length=237, Percent_Identity=29.535864978903, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI115533608, Length=184, Percent_Identity=29.3478260869565, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI116007184, Length=190, Percent_Identity=27.8947368421053, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI221500365, Length=190, Percent_Identity=27.8947368421053, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 28675; Mature: 28675

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSIDTYNACVDFPIFDAKSRSMKKAFLGAAGGAIGRNQDNVVVVEALKNVNLHLREGDR
CCEECCCCCEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHCCEEEEECCCE
VGLVGHNGAGKSTLLRLLSGIYEPTRGSADIRGRVAPVFDLGVGMDPEISGYENIIIRGL
EEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEE
FLGQTRKQMKAKMEEIADFTELGEYLSMPLRTYSTGMRIRLALGVVTSIEPEILLLDEGI
EHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEEEHHHCCCCCEEEEECCC
GAVDAAFMAKARDRLQALVERSGILVFASHSNDFLAQLCNTALWVDHGQIREAGLVPDVV
CHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHEECCCCCHHCCCCCHHHH
EAYEGKGAGDHVRRLLTRMEEEK
HHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVSIDTYNACVDFPIFDAKSRSMKKAFLGAAGGAIGRNQDNVVVVEALKNVNLHLREGDR
CCEECCCCCEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHCCEEEEECCCE
VGLVGHNGAGKSTLLRLLSGIYEPTRGSADIRGRVAPVFDLGVGMDPEISGYENIIIRGL
EEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEE
FLGQTRKQMKAKMEEIADFTELGEYLSMPLRTYSTGMRIRLALGVVTSIEPEILLLDEGI
EHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEEEHHHCCCCCEEEEECCC
GAVDAAFMAKARDRLQALVERSGILVFASHSNDFLAQLCNTALWVDHGQIREAGLVPDVV
CHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHEECCCCCHHCCCCCHHHH
EAYEGKGAGDHVRRLLTRMEEEK
HHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7692217 [H]