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The map label for this gene is iolD [H]

Identifier: 62389056

GI number: 62389056

Start: 173366

End: 175279

Strand: Direct

Name: iolD [H]

Synonym: cg0202

Alternate gene names: 62389056

Gene position: 173366-175279 (Clockwise)

Preceding gene: 62389055

Following gene: 62389057

Centisome position: 5.28

GC content: 56.53

Gene sequence:

>1914_bases
ATGGCTGAAACGAAGAGAATGACAGTTAGCCAGGCACTGGTTGAATTCCTTGGTCACCAGTGGACTGTCGACGGCGATAT
CCGCGAGCGCACCATTCCAGGCATGTTCGGAATTTTCGGACACGGAAACGTTGCTGGCATTGGCCAGGCACTCAAGCAGT
ACAACGTTGAACAACCTGAGCTCATGCCGTACTACCAGGCTCGTAATGAGCAGGCGATGGTGCACCAGTCTGTTGGATAT
GCACGCATGCACCGCCGTCGTGGCACATACGCATCTGCCGCATCTGTTGGACCCGGCGCGACCAACCTGTTAACCGGTGC
GGCTCTTGCTACCACCAACCGTTTGCCAGCGTTGCTGCTGCCTAGTGATACTTTTGCCACCCGCGTGGCGGATCCAGTGT
TGCAGCAGTTGGAGCAGCCATGGGATATCGGGCTGACGGTTAATGATGCTTTCCGCCCTGTGTCTAAGTTCTTTGATCGG
GTGCAGCGCCCGGAGCAGTTGTTCTCTATTGCGTTGGCTGCGATGCGTGTGTTGACTGATCCCGCAGAAACCGGTGCGGT
CACCATTGCGCTTCCAGAAGATGTGCAGGCTGAAATGCTCGATGTGCCGGTGGAGTTCTTGCAGGATCGTGAGTGGCACA
TTAGGCGCCCACGTCCAGAGCGTGCTGCGTTGGCTCGTGCGATTGAAGTCATCAAAAACGCTAAGAATCCGATGATCATT
GCTGGTGGCGGAGTGTTGTACTCCGATGCGGAAACGCAGCTGCAGGCACTTGTGGAGCAGACTGGCATTCCAGTGGGTAC
CTCCCAAGCTGGTGGTGGCGTGTTGGCGTGGGATCATGCACAAAACTTAGGTGGTGTGGGTGCCACCGGAACGTTGGCTG
CCAACCGCATTGCGGGTGATGCTGATGTGATCATCGGTATCGGTACTCGTTACAGCGATTTCACCACCGCATCTCGCACT
GCGTTCCAAAACCCTGATGTCACCTTCATCAACATCAATGTTGCTTCCTTCGATGCTTACAAGCATGGCACTCAGTTGCC
TGTGATTGCAGATGCACGCGAGGCAATTGTGGAGCTTGCTGAAGCCCTGCAGGGATTCACCGTGGCAGAGGATTACGCGC
AGCGCATCGCGAAGGAAAAGGCTGCGTGGGACGCAGAAGTAGATAAGTCTTTTGCCCCCTCCGGTCTTGCGCTGCCTGGA
CAGCCGGAGATCATCGGCGCGGTGCAGGCGTCGACAAGCGAAAAAGACGTCATTGTGCAGGCCGCTGGATCCTTGCCTGG
TGACCTGCACAAGCTGTGGCGTGTGCGCGATGCGCTGGGCTACCACGTGGAATATGCGTTCTCGTGCATGGGCTATGAAA
TCGCGGGCGGTATCGGCGCGAAGCGTGGACTTGATGCCGCAGGCGATGACCGCGACGTGGTGATCATGGTTGGTGATGGG
TCCTACCTCATGCTCAACACTGAGCTGGTCACGGCCGTGGCAGAAGGTATCAAGGTGATTGTGGTGCTCATCCAAAACCA
CGGTTATGCCTCCATCGGCCACCTGTCTGAAACTGTCGGTTCGCAGCGTTTTGGTACTTGGTACCGCGAATATGACGCTG
AGGCGAAAAACTTCCAGGGCGAGCAGATTCTGCCTGTTGACCTGGCGATGAATGCACGCAGCTACGGCATGGATGTCATT
GAAGTGGAACCAAGCGCGAATGCGATCGAGGATCTCAAAGCAGCGATGGCAACCGCGAAGGCTTCGGAGAAATCCACCTT
CATCCACATCAACAGCGATCCGTTGATCTACGCACCAGACGGTGCTGGTTGGTGGGACGTGCCGGTGTCGGAGACGTCCA
CTCTGGATAGCACCAACGCGGCTCGTGAAGATTACCTGAAAAACCAAGCCCTCCAGCGTCCGCTGCTCGGCTAA

Upstream 100 bases:

>100_bases
CGATGACCCAGCGCACGCCTGGGTTCGAGATACATGGACCGGGCAAGCATTTGATGATCGCTTGCCATATGAGAACGCAA
ACAAGGAGGGATAAAATTTC

Downstream 100 bases:

>100_bases
ACCAGTTGGCTAAACCAAAACGTATTTAAGGAGAAACACCATGACCACTTCTGTACCTGCATCCACCAAAGCTTCATCTG
TGGCTGGCGAAAACCCAGGC

Product: acetolactate synthase protein

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase 1 [H]

Number of amino acids: Translated: 637; Mature: 636

Protein sequence:

>637_residues
MAETKRMTVSQALVEFLGHQWTVDGDIRERTIPGMFGIFGHGNVAGIGQALKQYNVEQPELMPYYQARNEQAMVHQSVGY
ARMHRRRGTYASAASVGPGATNLLTGAALATTNRLPALLLPSDTFATRVADPVLQQLEQPWDIGLTVNDAFRPVSKFFDR
VQRPEQLFSIALAAMRVLTDPAETGAVTIALPEDVQAEMLDVPVEFLQDREWHIRRPRPERAALARAIEVIKNAKNPMII
AGGGVLYSDAETQLQALVEQTGIPVGTSQAGGGVLAWDHAQNLGGVGATGTLAANRIAGDADVIIGIGTRYSDFTTASRT
AFQNPDVTFININVASFDAYKHGTQLPVIADAREAIVELAEALQGFTVAEDYAQRIAKEKAAWDAEVDKSFAPSGLALPG
QPEIIGAVQASTSEKDVIVQAAGSLPGDLHKLWRVRDALGYHVEYAFSCMGYEIAGGIGAKRGLDAAGDDRDVVIMVGDG
SYLMLNTELVTAVAEGIKVIVVLIQNHGYASIGHLSETVGSQRFGTWYREYDAEAKNFQGEQILPVDLAMNARSYGMDVI
EVEPSANAIEDLKAAMATAKASEKSTFIHINSDPLIYAPDGAGWWDVPVSETSTLDSTNAAREDYLKNQALQRPLLG

Sequences:

>Translated_637_residues
MAETKRMTVSQALVEFLGHQWTVDGDIRERTIPGMFGIFGHGNVAGIGQALKQYNVEQPELMPYYQARNEQAMVHQSVGY
ARMHRRRGTYASAASVGPGATNLLTGAALATTNRLPALLLPSDTFATRVADPVLQQLEQPWDIGLTVNDAFRPVSKFFDR
VQRPEQLFSIALAAMRVLTDPAETGAVTIALPEDVQAEMLDVPVEFLQDREWHIRRPRPERAALARAIEVIKNAKNPMII
AGGGVLYSDAETQLQALVEQTGIPVGTSQAGGGVLAWDHAQNLGGVGATGTLAANRIAGDADVIIGIGTRYSDFTTASRT
AFQNPDVTFININVASFDAYKHGTQLPVIADAREAIVELAEALQGFTVAEDYAQRIAKEKAAWDAEVDKSFAPSGLALPG
QPEIIGAVQASTSEKDVIVQAAGSLPGDLHKLWRVRDALGYHVEYAFSCMGYEIAGGIGAKRGLDAAGDDRDVVIMVGDG
SYLMLNTELVTAVAEGIKVIVVLIQNHGYASIGHLSETVGSQRFGTWYREYDAEAKNFQGEQILPVDLAMNARSYGMDVI
EVEPSANAIEDLKAAMATAKASEKSTFIHINSDPLIYAPDGAGWWDVPVSETSTLDSTNAAREDYLKNQALQRPLLG
>Mature_636_residues
AETKRMTVSQALVEFLGHQWTVDGDIRERTIPGMFGIFGHGNVAGIGQALKQYNVEQPELMPYYQARNEQAMVHQSVGYA
RMHRRRGTYASAASVGPGATNLLTGAALATTNRLPALLLPSDTFATRVADPVLQQLEQPWDIGLTVNDAFRPVSKFFDRV
QRPEQLFSIALAAMRVLTDPAETGAVTIALPEDVQAEMLDVPVEFLQDREWHIRRPRPERAALARAIEVIKNAKNPMIIA
GGGVLYSDAETQLQALVEQTGIPVGTSQAGGGVLAWDHAQNLGGVGATGTLAANRIAGDADVIIGIGTRYSDFTTASRTA
FQNPDVTFININVASFDAYKHGTQLPVIADAREAIVELAEALQGFTVAEDYAQRIAKEKAAWDAEVDKSFAPSGLALPGQ
PEIIGAVQASTSEKDVIVQAAGSLPGDLHKLWRVRDALGYHVEYAFSCMGYEIAGGIGAKRGLDAAGDDRDVVIMVGDGS
YLMLNTELVTAVAEGIKVIVVLIQNHGYASIGHLSETVGSQRFGTWYREYDAEAKNFQGEQILPVDLAMNARSYGMDVIE
VEPSANAIEDLKAAMATAKASEKSTFIHINSDPLIYAPDGAGWWDVPVSETSTLDSTNAAREDYLKNQALQRPLLG

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=312, Percent_Identity=26.6025641025641, Blast_Score=78, Evalue=3e-14,
Organism=Homo sapiens, GI21361361, Length=512, Percent_Identity=24.21875, Blast_Score=75, Evalue=3e-13,
Organism=Escherichia coli, GI87081685, Length=541, Percent_Identity=25.3234750462107, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1790104, Length=511, Percent_Identity=24.4618395303327, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1786717, Length=529, Percent_Identity=24.5746691871456, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1787096, Length=429, Percent_Identity=22.1445221445221, Blast_Score=67, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17531299, Length=508, Percent_Identity=24.0157480314961, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17531301, Length=510, Percent_Identity=24.1176470588235, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323755, Length=476, Percent_Identity=23.9495798319328, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323073, Length=331, Percent_Identity=22.3564954682779, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 68650; Mature: 68519

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETKRMTVSQALVEFLGHQWTVDGDIRERTIPGMFGIFGHGNVAGIGQALKQYNVEQPE
CCCCHHHHHHHHHHHHHCCEEEECCCHHHHCCCCCEEECCCCCHHHHHHHHHHCCCCCCC
LMPYYQARNEQAMVHQSVGYARMHRRRGTYASAASVGPGATNLLTGAALATTNRLPALLL
CCCHHHCCCCHHHHHHHCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEE
PSDTFATRVADPVLQQLEQPWDIGLTVNDAFRPVSKFFDRVQRPEQLFSIALAAMRVLTD
CCCHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
PAETGAVTIALPEDVQAEMLDVPVEFLQDREWHIRRPRPERAALARAIEVIKNAKNPMII
CCHHCEEEEEECCCHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEE
AGGGVLYSDAETQLQALVEQTGIPVGTSQAGGGVLAWDHAQNLGGVGATGTLAANRIAGD
ECCCEEECCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHCCCC
ADVIIGIGTRYSDFTTASRTAFQNPDVTFININVASFDAYKHGTQLPVIADAREAIVELA
CEEEEEECCCCCCCCCHHHHHCCCCCEEEEEEEEEEEHHHHCCCCCCEEHHHHHHHHHHH
EALQGFTVAEDYAQRIAKEKAAWDAEVDKSFAPSGLALPGQPEIIGAVQASTSEKDVIVQ
HHHCCCCHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEE
AAGSLPGDLHKLWRVRDALGYHVEYAFSCMGYEIAGGIGAKRGLDAAGDDRDVVIMVGDG
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCEEEEEECCC
SYLMLNTELVTAVAEGIKVIVVLIQNHGYASIGHLSETVGSQRFGTWYREYDAEAKNFQG
CEEEEEHHHHHHHHHCCEEEEEEEECCCCEEHHHHHHHHCCHHHHHHHHHHCCHHHCCCC
EQILPVDLAMNARSYGMDVIEVEPSANAIEDLKAAMATAKASEKSTFIHINSDPLIYAPD
CEEEEEHEECCCHHHCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCC
GAGWWDVPVSETSTLDSTNAAREDYLKNQALQRPLLG
CCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AETKRMTVSQALVEFLGHQWTVDGDIRERTIPGMFGIFGHGNVAGIGQALKQYNVEQPE
CCCHHHHHHHHHHHHHCCEEEECCCHHHHCCCCCEEECCCCCHHHHHHHHHHCCCCCCC
LMPYYQARNEQAMVHQSVGYARMHRRRGTYASAASVGPGATNLLTGAALATTNRLPALLL
CCCHHHCCCCHHHHHHHCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEE
PSDTFATRVADPVLQQLEQPWDIGLTVNDAFRPVSKFFDRVQRPEQLFSIALAAMRVLTD
CCCHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
PAETGAVTIALPEDVQAEMLDVPVEFLQDREWHIRRPRPERAALARAIEVIKNAKNPMII
CCHHCEEEEEECCCHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEE
AGGGVLYSDAETQLQALVEQTGIPVGTSQAGGGVLAWDHAQNLGGVGATGTLAANRIAGD
ECCCEEECCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHCCCC
ADVIIGIGTRYSDFTTASRTAFQNPDVTFININVASFDAYKHGTQLPVIADAREAIVELA
CEEEEEECCCCCCCCCHHHHHCCCCCEEEEEEEEEEEHHHHCCCCCCEEHHHHHHHHHHH
EALQGFTVAEDYAQRIAKEKAAWDAEVDKSFAPSGLALPGQPEIIGAVQASTSEKDVIVQ
HHHCCCCHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEE
AAGSLPGDLHKLWRVRDALGYHVEYAFSCMGYEIAGGIGAKRGLDAAGDDRDVVIMVGDG
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCEEEEEECCC
SYLMLNTELVTAVAEGIKVIVVLIQNHGYASIGHLSETVGSQRFGTWYREYDAEAKNFQG
CEEEEEHHHHHHHHHCCEEEEEEEECCCCEEHHHHHHHHCCHHHHHHHHHHCCHHHCCCC
EQILPVDLAMNARSYGMDVIEVEPSANAIEDLKAAMATAKASEKSTFIHINSDPLIYAPD
CEEEEEHEECCCHHHCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCC
GAGWWDVPVSETSTLDSTNAAREDYLKNQALQRPLLG
CCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; Decarboxylation [C]

Inhibitor: 2-oxobutanoate; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA