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The map label for this gene is pnpA

Identifier: 62185343

GI number: 62185343

Start: 840274

End: 842358

Strand: Direct

Name: pnpA

Synonym: CAB730

Alternate gene names: 62185343

Gene position: 840274-842358 (Clockwise)

Preceding gene: 62185342

Following gene: 62185346

Centisome position: 73.43

GC content: 41.01

Gene sequence:

>2085_bases
ATGACATTTGAAACTATTTCTGTTACCCTTGAAGAAGGCAAAACGTTAGTATTTGAAACGGGGAAAATAGCGCGTCAGGC
TAACGGCGCAGTTCTTGCTCGCATGCAAGAAACATGGGTCTTTTCTTCTGTTTGTGCGGCTAACCTTGAGGAAGCTGTTG
ACTTCCTACCTCTAAGAGTTGACTACCAAGAAAAGTTTTCCTCTATAGGAAAAACTTTAGGCGGGTTTATTAAAAGGGAA
GGTCGCCCAACAGAAAGAGAAATTTTAACTTCTCGCCTGATAGATCGCTCCATGCGTCCTTCGTTACCCAACCGGTTAAT
GCAGGACATTCAAATTTTATCTTACGTATGGTCATACGACGGAGTCACTCTTCCTGATCCCATCGCAATTTGCGGTGTTT
CTGCAGCCTTAGCAATTTCTGACATTCCTCAGACTAGTATTGTCGCTGGTGTTCGTGTCGGCTTTGTGAACAATCGTTGG
GTAGTTAACCCAACAAAAGCGGAAATGGACGTTTCCAGAATGGAACTCGTTTTAGCAGGAACAGAAAATGCTATCCTAAT
GATCGAGGGACACTGCGACTTCTTCACAGAAGAGCAAGTTATCGAAGCTATTGAGTTTGGTCACAAACACATTGTTACTA
TATGCAAGGCACTAAAGGATTGGCAAAAACACATAGGTAAAGAAAAAAATACAAGTGGAATTGTTTCTCTTCCAGAGGAA
GTCCAATCTGCCGTAAACACATTTGTGGAAGGAAAGTTTGTTGACCTCCTGAAAATTAAGGAAAAGAAAGCTTTCGAGGC
TGCTTCAAAACAGTTAGAAAATGAAGTCATCGAACAGCTCCAAGAAGAAAATGAAGTCTTTACTGCGTTCAATATTAAAA
TTGCTTTTAAGCAAGCAAAGTCTAACTTCATACGTGGTTTAATACGTGAACAAGGTCTACGTTCTGATGGACGTTCTGTA
ACGACTATTCGTCCTATCTCTATTGATACGTCTTTCCTCCCCAGGACTCACGGCAGTTGCTTATTTACTCGGGGAGAAAC
TCAAACTATGGCTGTCTGTACGTTAGGTAGCGAAGCCATGGCCCAACGGTATGAAGACTTAAATGGTGAAGGATTAGCCA
AATTTTACTTACAATACTTCTTCCCTCCCTTTTCTGTTGGAGAAGTAGGAAGGATCGGCTCTCCAGGAAGAAGAGAAATC
GGTCACGGTAAACTTGCGGAAAAAGCATTAAGTCACACGCTACCCGATCCTATGAAGTTCCCCTATACTATCCGCATAGA
ATCCAATATTACAGAATCTAATGGTTCTTCATCTATGGCATCTGTTTGCGGAGGTTGTTTAGCTTTAATGGATGCTGGGG
TGCCTATTAAAACTCCTATAGCTGGTATAGCCATGGGACTAATATTAGAGGATGACCATGTAACTATTCTCTCAGATATT
TCCGGATTAGAAGATCATTTAGGTGATATGGATTTCAAGGTTGCTGGAAATACCGAAGGAATTACAGCATTTCAAATGGA
TATTAAAGTAGAAGGCATTACTCCTGATATTATGCGAGCTGCCCTAGCCCAAGCTAAAGAAGGTCGTCAAGATATTCTTG
AAACCATGAAACAAGCTCTTGCGGCTCCTAAAACCGACTTATCCCAATACGCTCCTCGCATTGAAACAATGCAAATTAAG
CCGAATAAAATTGCTACTGTTATTGGGCCCGGAGGTAAACAGATTCGTCAAATTATTGAAGAAGCTGGAGTACAAATTGA
TATTAATGACTCGGGTCTTGTCAGTATTTCTGCCTCTTCACCACAAGCCATAGAGAAAGCTAAATCTATTATTGAAGGCT
TAGTTGGCGAAGTTGAAGTAGGTAAGATTTATGAAGGTCGCGTAACATCTGTTGTCCCTTTTGGAGCCTTTGTTGAAATT
CTCCCTGGTAAAGAAGGTCTTTGCCATATTTCTGAATTTTCTAAACAACGTATAGACAATGTTGGTGATTTCGTTAAACA
GGGGGACACCCTAACTGTTAAACTTTTAAGCATCAACGAAAAAGGACAGTACAAACTCAGTCATAAAGCTACTCTCAGTG
AGTAG

Upstream 100 bases:

>100_bases
CAGATACCGAAAGATATAAAAATTTAATTTCAAGATTAAATCTAAGAAAATAATCTTTTATTTATTTTCCCCGGCAAAAA
AATCTAATTAGGAGACCTTC

Downstream 100 bases:

>100_bases
TCCCCCCCTTTTTCCTTTATTGTTTTTCTTTAGATCTTTTTGAAAAGATAAAAAAAAAGCCCCTAGTTAGGGGCTTTTCA
CATCAGTACATAATCTATAG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 694; Mature: 693

Protein sequence:

>694_residues
MTFETISVTLEEGKTLVFETGKIARQANGAVLARMQETWVFSSVCAANLEEAVDFLPLRVDYQEKFSSIGKTLGGFIKRE
GRPTEREILTSRLIDRSMRPSLPNRLMQDIQILSYVWSYDGVTLPDPIAICGVSAALAISDIPQTSIVAGVRVGFVNNRW
VVNPTKAEMDVSRMELVLAGTENAILMIEGHCDFFTEEQVIEAIEFGHKHIVTICKALKDWQKHIGKEKNTSGIVSLPEE
VQSAVNTFVEGKFVDLLKIKEKKAFEAASKQLENEVIEQLQEENEVFTAFNIKIAFKQAKSNFIRGLIREQGLRSDGRSV
TTIRPISIDTSFLPRTHGSCLFTRGETQTMAVCTLGSEAMAQRYEDLNGEGLAKFYLQYFFPPFSVGEVGRIGSPGRREI
GHGKLAEKALSHTLPDPMKFPYTIRIESNITESNGSSSMASVCGGCLALMDAGVPIKTPIAGIAMGLILEDDHVTILSDI
SGLEDHLGDMDFKVAGNTEGITAFQMDIKVEGITPDIMRAALAQAKEGRQDILETMKQALAAPKTDLSQYAPRIETMQIK
PNKIATVIGPGGKQIRQIIEEAGVQIDINDSGLVSISASSPQAIEKAKSIIEGLVGEVEVGKIYEGRVTSVVPFGAFVEI
LPGKEGLCHISEFSKQRIDNVGDFVKQGDTLTVKLLSINEKGQYKLSHKATLSE

Sequences:

>Translated_694_residues
MTFETISVTLEEGKTLVFETGKIARQANGAVLARMQETWVFSSVCAANLEEAVDFLPLRVDYQEKFSSIGKTLGGFIKRE
GRPTEREILTSRLIDRSMRPSLPNRLMQDIQILSYVWSYDGVTLPDPIAICGVSAALAISDIPQTSIVAGVRVGFVNNRW
VVNPTKAEMDVSRMELVLAGTENAILMIEGHCDFFTEEQVIEAIEFGHKHIVTICKALKDWQKHIGKEKNTSGIVSLPEE
VQSAVNTFVEGKFVDLLKIKEKKAFEAASKQLENEVIEQLQEENEVFTAFNIKIAFKQAKSNFIRGLIREQGLRSDGRSV
TTIRPISIDTSFLPRTHGSCLFTRGETQTMAVCTLGSEAMAQRYEDLNGEGLAKFYLQYFFPPFSVGEVGRIGSPGRREI
GHGKLAEKALSHTLPDPMKFPYTIRIESNITESNGSSSMASVCGGCLALMDAGVPIKTPIAGIAMGLILEDDHVTILSDI
SGLEDHLGDMDFKVAGNTEGITAFQMDIKVEGITPDIMRAALAQAKEGRQDILETMKQALAAPKTDLSQYAPRIETMQIK
PNKIATVIGPGGKQIRQIIEEAGVQIDINDSGLVSISASSPQAIEKAKSIIEGLVGEVEVGKIYEGRVTSVVPFGAFVEI
LPGKEGLCHISEFSKQRIDNVGDFVKQGDTLTVKLLSINEKGQYKLSHKATLSE
>Mature_693_residues
TFETISVTLEEGKTLVFETGKIARQANGAVLARMQETWVFSSVCAANLEEAVDFLPLRVDYQEKFSSIGKTLGGFIKREG
RPTEREILTSRLIDRSMRPSLPNRLMQDIQILSYVWSYDGVTLPDPIAICGVSAALAISDIPQTSIVAGVRVGFVNNRWV
VNPTKAEMDVSRMELVLAGTENAILMIEGHCDFFTEEQVIEAIEFGHKHIVTICKALKDWQKHIGKEKNTSGIVSLPEEV
QSAVNTFVEGKFVDLLKIKEKKAFEAASKQLENEVIEQLQEENEVFTAFNIKIAFKQAKSNFIRGLIREQGLRSDGRSVT
TIRPISIDTSFLPRTHGSCLFTRGETQTMAVCTLGSEAMAQRYEDLNGEGLAKFYLQYFFPPFSVGEVGRIGSPGRREIG
HGKLAEKALSHTLPDPMKFPYTIRIESNITESNGSSSMASVCGGCLALMDAGVPIKTPIAGIAMGLILEDDHVTILSDIS
GLEDHLGDMDFKVAGNTEGITAFQMDIKVEGITPDIMRAALAQAKEGRQDILETMKQALAAPKTDLSQYAPRIETMQIKP
NKIATVIGPGGKQIRQIIEEAGVQIDINDSGLVSISASSPQAIEKAKSIIEGLVGEVEVGKIYEGRVTSVVPFGAFVEIL
PGKEGLCHISEFSKQRIDNVGDFVKQGDTLTVKLLSINEKGQYKLSHKATLSE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=702, Percent_Identity=34.1880341880342, Blast_Score=396, Evalue=1e-110,
Organism=Escherichia coli, GI145693187, Length=676, Percent_Identity=45.1183431952663, Blast_Score=600, Evalue=1e-173,
Organism=Caenorhabditis elegans, GI115534063, Length=712, Percent_Identity=30.1966292134831, Blast_Score=292, Evalue=4e-79,
Organism=Drosophila melanogaster, GI281362905, Length=678, Percent_Identity=35.3982300884956, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24651641, Length=678, Percent_Identity=35.3982300884956, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24651643, Length=678, Percent_Identity=35.3982300884956, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI161079377, Length=616, Percent_Identity=36.038961038961, Blast_Score=382, Evalue=1e-106,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_CHLAB (Q5L5B4)

Other databases:

- EMBL:   CR848038
- RefSeq:   YP_220128.1
- HSSP:   P05055
- ProteinModelPortal:   Q5L5B4
- GeneID:   3338065
- GenomeReviews:   CR848038_GR
- KEGG:   cab:CAB730
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- PhylomeDB:   Q5L5B4
- ProtClustDB:   PRK11824
- BioCyc:   CABO218497:CAB730-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 75996; Mature: 75865

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFETISVTLEEGKTLVFETGKIARQANGAVLARMQETWVFSSVCAANLEEAVDFLPLRV
CCEEEEEEEEECCCEEEEECCHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHEEEEE
DYQEKFSSIGKTLGGFIKREGRPTEREILTSRLIDRSMRPSLPNRLMQDIQILSYVWSYD
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
GVTLPDPIAICGVSAALAISDIPQTSIVAGVRVGFVNNRWVVNPTKAEMDVSRMELVLAG
CCCCCCCHHHHCCHHHHHHCCCCCHHHHHHEEEEEECCEEEECCCHHHCCHHHEEEEEEC
TENAILMIEGHCDFFTEEQVIEAIEFGHKHIVTICKALKDWQKHIGKEKNTSGIVSLPEE
CCCEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCEECHHH
VQSAVNTFVEGKFVDLLKIKEKKAFEAASKQLENEVIEQLQEENEVFTAFNIKIAFKQAK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEHHHH
SNFIRGLIREQGLRSDGRSVTTIRPISIDTSFLPRTHGSCLFTRGETQTMAVCTLGSEAM
HHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCEEEECCCCCEEEEEECCHHHH
AQRYEDLNGEGLAKFYLQYFFPPFSVGEVGRIGSPGRREIGHGKLAEKALSHTLPDPMKF
HHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
PYTIRIESNITESNGSSSMASVCGGCLALMDAGVPIKTPIAGIAMGLILEDDHVTILSDI
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHEEEEEECCCEEEEHHH
SGLEDHLGDMDFKVAGNTEGITAFQMDIKVEGITPDIMRAALAQAKEGRQDILETMKQAL
CHHHHHHCCCCEEEECCCCCEEEEEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHH
AAPKTDLSQYAPRIETMQIKPNKIATVIGPGGKQIRQIIEEAGVQIDINDSGLVSISASS
CCCCCCHHHHCCCCEEEEECCCCEEEEECCCHHHHHHHHHHCCCEEEECCCCEEEEECCC
PQAIEKAKSIIEGLVGEVEVGKIYEGRVTSVVPFGAFVEILPGKEGLCHISEFSKQRIDN
CHHHHHHHHHHHHHHCCEEECEEECCCCCEEECCCHHHEECCCCCCCEEHHHHHHHHHHH
VGDFVKQGDTLTVKLLSINEKGQYKLSHKATLSE
HHHHHHCCCEEEEEEEEECCCCCEEEECCCCCCC
>Mature Secondary Structure 
TFETISVTLEEGKTLVFETGKIARQANGAVLARMQETWVFSSVCAANLEEAVDFLPLRV
CEEEEEEEEECCCEEEEECCHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHEEEEE
DYQEKFSSIGKTLGGFIKREGRPTEREILTSRLIDRSMRPSLPNRLMQDIQILSYVWSYD
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
GVTLPDPIAICGVSAALAISDIPQTSIVAGVRVGFVNNRWVVNPTKAEMDVSRMELVLAG
CCCCCCCHHHHCCHHHHHHCCCCCHHHHHHEEEEEECCEEEECCCHHHCCHHHEEEEEEC
TENAILMIEGHCDFFTEEQVIEAIEFGHKHIVTICKALKDWQKHIGKEKNTSGIVSLPEE
CCCEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCEECHHH
VQSAVNTFVEGKFVDLLKIKEKKAFEAASKQLENEVIEQLQEENEVFTAFNIKIAFKQAK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEHHHH
SNFIRGLIREQGLRSDGRSVTTIRPISIDTSFLPRTHGSCLFTRGETQTMAVCTLGSEAM
HHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCEEEECCCCCEEEEEECCHHHH
AQRYEDLNGEGLAKFYLQYFFPPFSVGEVGRIGSPGRREIGHGKLAEKALSHTLPDPMKF
HHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
PYTIRIESNITESNGSSSMASVCGGCLALMDAGVPIKTPIAGIAMGLILEDDHVTILSDI
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHEEEEEECCCEEEEHHH
SGLEDHLGDMDFKVAGNTEGITAFQMDIKVEGITPDIMRAALAQAKEGRQDILETMKQAL
CHHHHHHCCCCEEEECCCCCEEEEEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHH
AAPKTDLSQYAPRIETMQIKPNKIATVIGPGGKQIRQIIEEAGVQIDINDSGLVSISASS
CCCCCCHHHHCCCCEEEEECCCCEEEEECCCHHHHHHHHHHCCCEEEECCCCEEEEECCC
PQAIEKAKSIIEGLVGEVEVGKIYEGRVTSVVPFGAFVEILPGKEGLCHISEFSKQRIDN
CHHHHHHHHHHHHHHCCEEECEEECCCCCEEECCCHHHEECCCCCCCEEHHHHHHHHHHH
VGDFVKQGDTLTVKLLSINEKGQYKLSHKATLSE
HHHHHHCCCEEEEEEEEECCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA