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The map label for this gene is pfkA [H]

Identifier: 62185199

GI number: 62185199

Start: 661812

End: 663437

Strand: Direct

Name: pfkA [H]

Synonym: CAB583

Alternate gene names: 62185199

Gene position: 661812-663437 (Clockwise)

Preceding gene: 62185198

Following gene: 62185202

Centisome position: 57.83

GC content: 39.05

Gene sequence:

>1626_bases
GTGGAGCTTCTCTCTGTAAATAAAAGTTACTTTGAATTGCAACGTTTGCACTACCGTCCGGAAACCTTAAGATTTTTACA
GGGTATCACATCTCTGCACATTGTAGACTCTGTCTCTACAGCCTCTCCTCAGGTTCCTAAAGATCTTCAAGAACATATTC
CTTATTTGTGCAATATCCCTGAAGTCACTATTGAAAAGGGAAAAGCAAAGCCTTCCCAACCTTTGAAGATTGGGGTATTA
CTTTCTGGGGGTCAGGCTCCCGGAGGCCACAACGTTGTTATAGGGCTATTCGAAGGACTGCGCGCCTTTAACCCTAAAAC
AAAACTTTTTGGGTTTATCCAAGGCCCTTTAGGATTAACACGTGGTTTATATAAAGATCTCGATATCTCTGTGATCTACG
ACTATTACAATGTCGGGGGTTTTGACATGCTCTCTTCAAGTAAAGAGAAAATCAAAACCAAGGAACAAAAAAGCACCATT
CTTACTACTGTAAAAAAGCTCAAACTTGATGGTCTACTGATTATAGGAGGGAATGATTCTAATACCGATACAGCCATGCT
CGCGGAGTATTTCCTCAAGAATAATTGCCATATCCCCATCATTGGGGTGCCAAAAACTATTGATGGAGATCTAAAAAACT
TCTGGATTGAGACCCCCTTAGGGTTTCATACTTCCTGTCGCATTTACTCAGAAATGATCGGAAATTTAGAAAAAGACGCT
CTATCCATAAAAAAGTACCATCATTTTGTCCGTCTTATGGGACAAAAAGCTTCTTACACAACTTTAGAATGTGGTCTACA
GACTTTACCTAATATCACCCTCATTAGCGAACATCTCGCTATGAGAAAGATTTCCCTACAAAAGCTTAGTGAACATCTCG
CTATGGGCTTAGTGAATCGCTACAGATCTGGGAAAAATTATAGTACGATACTGATTCCTGAAGGGTTGCTTGAGCATATA
TTCGATACAAAGAAACTCATCAATGAACTCAATGTTTTGCTTTTAGACAATACCCTCAACTTTGATAATGTAAATAAACA
ACTTTCTCCAGAGTCTTTAAAGACATTTTCTGCTCTACCTACAGAAATTGCTCAGCAACTACTGATCGCTAGGGATTCTT
ATGGGAATGTACGGGTCTCTAAAATTGCTACAGAGGAACTCTTAGCTGCTCTAATAAAAAAAGAAATCCAAAAAATAGAA
CCAAAAATGGAATTCCAATCTGTGAATCACTTTTTTGGCTATGAGTCGCGTGCAGGATTCCCTTCAAATTTCGATGCCAA
TTACGGACTCGCCTTAGGCATTGTTTCTGCTCTTTTCCTTGTCAGACAACGGACAGGTTATATGGTAACTATCAACAACC
TGGCTCTCCCTTATAGCGAATGGAGTGGTGGGGCTACGCCTTTATATAAAATGATGCAATTGGAACAACGCTTAGGTGAA
GAAATCCCTGTAATTAAAACAGATTCTGTAAATCCAAATGCTCCGGAAGTTCAATACCTTTTAAATCAAAGTGATACTTG
CTTGATGAAAGATATGTATTCTTTTCCGGGGCCATTACAATATTTTGGTGAAGAACGACTCATAGATCAACGACCACTAA
CATTGTTATGGGGAAATAAAACATAG

Upstream 100 bases:

>100_bases
CGTCTATCCCCATACGGGCCACCATATTCCTCAGTCTTGCTCTATGTTATCGGAACTTGTACAATGGCTAAAACATCAAC
TTATTCCCTAGGAACTACCT

Downstream 100 bases:

>100_bases
AGAAAAATAAGCACCTTTGCCAAGGCAACTATCTGGCCACTTCAGAAACCACTCTCTAAAAAGATGCTTATAGAAGAAAA
TTAGCGGTAGTCGGACTTGA

Product: diphosphate--fructose-6-phosphate 1-phosphotransferase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MELLSVNKSYFELQRLHYRPETLRFLQGITSLHIVDSVSTASPQVPKDLQEHIPYLCNIPEVTIEKGKAKPSQPLKIGVL
LSGGQAPGGHNVVIGLFEGLRAFNPKTKLFGFIQGPLGLTRGLYKDLDISVIYDYYNVGGFDMLSSSKEKIKTKEQKSTI
LTTVKKLKLDGLLIIGGNDSNTDTAMLAEYFLKNNCHIPIIGVPKTIDGDLKNFWIETPLGFHTSCRIYSEMIGNLEKDA
LSIKKYHHFVRLMGQKASYTTLECGLQTLPNITLISEHLAMRKISLQKLSEHLAMGLVNRYRSGKNYSTILIPEGLLEHI
FDTKKLINELNVLLLDNTLNFDNVNKQLSPESLKTFSALPTEIAQQLLIARDSYGNVRVSKIATEELLAALIKKEIQKIE
PKMEFQSVNHFFGYESRAGFPSNFDANYGLALGIVSALFLVRQRTGYMVTINNLALPYSEWSGGATPLYKMMQLEQRLGE
EIPVIKTDSVNPNAPEVQYLLNQSDTCLMKDMYSFPGPLQYFGEERLIDQRPLTLLWGNKT

Sequences:

>Translated_541_residues
MELLSVNKSYFELQRLHYRPETLRFLQGITSLHIVDSVSTASPQVPKDLQEHIPYLCNIPEVTIEKGKAKPSQPLKIGVL
LSGGQAPGGHNVVIGLFEGLRAFNPKTKLFGFIQGPLGLTRGLYKDLDISVIYDYYNVGGFDMLSSSKEKIKTKEQKSTI
LTTVKKLKLDGLLIIGGNDSNTDTAMLAEYFLKNNCHIPIIGVPKTIDGDLKNFWIETPLGFHTSCRIYSEMIGNLEKDA
LSIKKYHHFVRLMGQKASYTTLECGLQTLPNITLISEHLAMRKISLQKLSEHLAMGLVNRYRSGKNYSTILIPEGLLEHI
FDTKKLINELNVLLLDNTLNFDNVNKQLSPESLKTFSALPTEIAQQLLIARDSYGNVRVSKIATEELLAALIKKEIQKIE
PKMEFQSVNHFFGYESRAGFPSNFDANYGLALGIVSALFLVRQRTGYMVTINNLALPYSEWSGGATPLYKMMQLEQRLGE
EIPVIKTDSVNPNAPEVQYLLNQSDTCLMKDMYSFPGPLQYFGEERLIDQRPLTLLWGNKT
>Mature_541_residues
MELLSVNKSYFELQRLHYRPETLRFLQGITSLHIVDSVSTASPQVPKDLQEHIPYLCNIPEVTIEKGKAKPSQPLKIGVL
LSGGQAPGGHNVVIGLFEGLRAFNPKTKLFGFIQGPLGLTRGLYKDLDISVIYDYYNVGGFDMLSSSKEKIKTKEQKSTI
LTTVKKLKLDGLLIIGGNDSNTDTAMLAEYFLKNNCHIPIIGVPKTIDGDLKNFWIETPLGFHTSCRIYSEMIGNLEKDA
LSIKKYHHFVRLMGQKASYTTLECGLQTLPNITLISEHLAMRKISLQKLSEHLAMGLVNRYRSGKNYSTILIPEGLLEHI
FDTKKLINELNVLLLDNTLNFDNVNKQLSPESLKTFSALPTEIAQQLLIARDSYGNVRVSKIATEELLAALIKKEIQKIE
PKMEFQSVNHFFGYESRAGFPSNFDANYGLALGIVSALFLVRQRTGYMVTINNLALPYSEWSGGATPLYKMMQLEQRLGE
EIPVIKTDSVNPNAPEVQYLLNQSDTCLMKDMYSFPGPLQYFGEERLIDQRPLTLLWGNKT

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Escherichia coli, GI1790350, Length=251, Percent_Identity=27.0916334661355, Blast_Score=73, Evalue=6e-14,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 60818; Mature: 60818

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLSVNKSYFELQRLHYRPETLRFLQGITSLHIVDSVSTASPQVPKDLQEHIPYLCNIP
CCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHCCCEEECCC
EVTIEKGKAKPSQPLKIGVLLSGGQAPGGHNVVIGLFEGLRAFNPKTKLFGFIQGPLGLT
CCEEECCCCCCCCCEEEEEEEECCCCCCCCCEEEEHHHHHHHCCCCHHEEEEEECCHHHH
RGLYKDLDISVIYDYYNVGGFDMLSSSKEKIKTKEQKSTILTTVKKLKLDGLLIIGGNDS
HHHHHCCCEEEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
NTDTAMLAEYFLKNNCHIPIIGVPKTIDGDLKNFWIETPLGFHTSCRIYSEMIGNLEKDA
CCHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHEEECCCCCCCHHHHHHHHHHCCHHHHH
LSIKKYHHFVRLMGQKASYTTLECGLQTLPNITLISEHLAMRKISLQKLSEHLAMGLVNR
HHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRSGKNYSTILIPEGLLEHIFDTKKLINELNVLLLDNTLNFDNVNKQLSPESLKTFSALP
HCCCCCCEEEECCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHCH
TEIAQQLLIARDSYGNVRVSKIATEELLAALIKKEIQKIEPKMEFQSVNHFFGYESRAGF
HHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCC
PSNFDANYGLALGIVSALFLVRQRTGYMVTINNLALPYSEWSGGATPLYKMMQLEQRLGE
CCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCEECCCCCCCCCCHHHHHHHHHHHHCCC
EIPVIKTDSVNPNAPEVQYLLNQSDTCLMKDMYSFPGPLQYFGEERLIDQRPLTLLWGNK
CCCEEEECCCCCCCCHHHHHHCCCCHHHHHHHHCCCCHHHHCCCHHCCCCCCEEEEECCC
T
C
>Mature Secondary Structure
MELLSVNKSYFELQRLHYRPETLRFLQGITSLHIVDSVSTASPQVPKDLQEHIPYLCNIP
CCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHCCCEEECCC
EVTIEKGKAKPSQPLKIGVLLSGGQAPGGHNVVIGLFEGLRAFNPKTKLFGFIQGPLGLT
CCEEECCCCCCCCCEEEEEEEECCCCCCCCCEEEEHHHHHHHCCCCHHEEEEEECCHHHH
RGLYKDLDISVIYDYYNVGGFDMLSSSKEKIKTKEQKSTILTTVKKLKLDGLLIIGGNDS
HHHHHCCCEEEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
NTDTAMLAEYFLKNNCHIPIIGVPKTIDGDLKNFWIETPLGFHTSCRIYSEMIGNLEKDA
CCHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHEEECCCCCCCHHHHHHHHHHCCHHHHH
LSIKKYHHFVRLMGQKASYTTLECGLQTLPNITLISEHLAMRKISLQKLSEHLAMGLVNR
HHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRSGKNYSTILIPEGLLEHIFDTKKLINELNVLLLDNTLNFDNVNKQLSPESLKTFSALP
HCCCCCCEEEECCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHCH
TEIAQQLLIARDSYGNVRVSKIATEELLAALIKKEIQKIEPKMEFQSVNHFFGYESRAGF
HHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCC
PSNFDANYGLALGIVSALFLVRQRTGYMVTINNLALPYSEWSGGATPLYKMMQLEQRLGE
CCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCEECCCCCCCCCCHHHHHHHHHHHHCCC
EIPVIKTDSVNPNAPEVQYLLNQSDTCLMKDMYSFPGPLQYFGEERLIDQRPLTLLWGNK
CCCEEEECCCCCCCCHHHHHHCCCCHHHHHHHHCCCCHHHHCCCHHCCCCCCEEEEECCC
T
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA