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The map label for this gene is pfkA [H]

Identifier: 62185197

GI number: 62185197

Start: 659209

End: 660867

Strand: Direct

Name: pfkA [H]

Synonym: CAB581

Alternate gene names: 62185197

Gene position: 659209-660867 (Clockwise)

Preceding gene: 62185196

Following gene: 62185198

Centisome position: 57.6

GC content: 40.2

Gene sequence:

>1659_bases
ATGCAATCGAACTCCAATGATCTCAATGACGTAGTTATCCCAGGCACCCCGCCACTCCCCAGGGAACTACAAAAATTCCC
CTCTTTGATTCCAACAAATGATTTACGATTTTCCCCTAAGTTTGATGCCGACGTTGCGAAGCTTTTCCCTAATACCTATG
ACAGTCCTTACCTAAAATTTACTTCAGGAACTCCTGACCAACCTGTTCGCCCACTAAAAGTCGGTGTGATGCTATCGGGA
GGTCCCGCTCCCGGAGGTCATAACGTTATTTGGGGATTACTTCACAGCTTAAAGAAAATTCATCCCGACAGTTCGCTCAT
CGGTTTTCTTAATAACGGCCAGGGGATTCTAAATAATCATACAATAGAAATTACCGAAGAATTCATGGAGTGTTTCAAAA
ATTCCGGAGGATTCAACTGCATAGGCACAGGAAGAACAAATATCATCACGGAAGAAAATAAAGCTGTATGTTTAAAAACA
GTACAGGCTTTAGACCTGGATGGTTTGGTCATTATTGGCGGTGATGGTTCGAATACAGCAACTGCTATCCTAGCAGAATA
CTTTTCTCAGCATCATCCTAAAACGTGCGTTGTAGGCGTCCCTAAAACTATTGATGGTGATCTTCAACACCTATTTTTAG
ACCTCACCTTTGGATTTGATTCAGCAACAAAATTCTATTCCTCGATTATTAGCAACATTTCTAGAGATACGCTATCTTGC
AAAGCGCATTACCACGTGATCAAGCTGATGGGCCGCTCTGCATCGCACATTGCTTTAGAATGCACTCTACAAACACATCC
AAATATCACTCTTATAGGAGAAGAAATCGCAGAAAAGAATGTTCCTCTAAATACGATCATCCATAAAGTCTGTTCCATCA
TTGCTGATAGAGCCGCTATGGGGAAATACTACGGCATCATTCTTATTCCTGAAGGCATTATCGAATTCATCCCCGAGATT
AACAACCTGGTGAAAGAAATCGAAAGAATCCCTGAGCATGCTGATAAATTTTCTTCTCTATCCCGAGAATCTCAAAAATT
ACTAAAAAGCTTCCCGGAAACTATCGCTAAACAACTCCTGAATGATCGTGATGCTCACGGGAACGTCTATGTATCTAAAA
TCAGCGTGGACAAATTGCTTATCCACCTCGTAGATAACCACCTGAAGAAACATTTTAAAAATGTTCCGTTTAATGCGATT
TCTCATTTTTTAGGTTATGAGGGAAGGTCCTGCTTACCAACAAAATTCGATAATACCTACAGCTACGCCTTAGGCTATGG
CGCAGGAGTTCTTACTTATAATCGCTGCAACGGCTACCTCACTGTCATCGAATCACTTATCAATATTGTAGATAAATGGC
GGCTACGCGCTATGCCTATTGTAAAAATGTTCACAACAAAGAAAAAGTCGGATGGAACTATAAAACCTCTGATAAAAAAG
AGTTTAATTGATATTAGCAGTCCTGCTTTTGTGAAGTTTAAACTCTATAGAAAGATATGGGCTCTAGAAGATTCTTATCG
TGTTTTAGGACCATTACAGATTGACACCCCACCAAACTCTCATTCCGATCATTTTCCTCCTCTTACTCTCCTCCTCAATC
ATAATGAATGGCAAAAAAGGTGTTCTATATGTATGGAAATCCCTGACTGTGATTATTAA

Upstream 100 bases:

>100_bases
TCAAACAGCGAGAAAGAAAACCTTCTAACCAGAAGGTTTTCTTTTATTCGATAAAGAAAAACACAGCGAATTTACGAAAA
TACTGTATCCATTAACTGTT

Downstream 100 bases:

>100_bases
TTTCCACTCGCGATATCACACGATGATGCCATAAGAAAGTGGAGTTTATTATCGACGAATTCTTTTTAAAAAATTAAATT
TACGCATAAATACCGTCTTC

Product: diphosphate--fructose-6-phosphate 1-phosphotransferase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MQSNSNDLNDVVIPGTPPLPRELQKFPSLIPTNDLRFSPKFDADVAKLFPNTYDSPYLKFTSGTPDQPVRPLKVGVMLSG
GPAPGGHNVIWGLLHSLKKIHPDSSLIGFLNNGQGILNNHTIEITEEFMECFKNSGGFNCIGTGRTNIITEENKAVCLKT
VQALDLDGLVIIGGDGSNTATAILAEYFSQHHPKTCVVGVPKTIDGDLQHLFLDLTFGFDSATKFYSSIISNISRDTLSC
KAHYHVIKLMGRSASHIALECTLQTHPNITLIGEEIAEKNVPLNTIIHKVCSIIADRAAMGKYYGIILIPEGIIEFIPEI
NNLVKEIERIPEHADKFSSLSRESQKLLKSFPETIAKQLLNDRDAHGNVYVSKISVDKLLIHLVDNHLKKHFKNVPFNAI
SHFLGYEGRSCLPTKFDNTYSYALGYGAGVLTYNRCNGYLTVIESLINIVDKWRLRAMPIVKMFTTKKKSDGTIKPLIKK
SLIDISSPAFVKFKLYRKIWALEDSYRVLGPLQIDTPPNSHSDHFPPLTLLLNHNEWQKRCSICMEIPDCDY

Sequences:

>Translated_552_residues
MQSNSNDLNDVVIPGTPPLPRELQKFPSLIPTNDLRFSPKFDADVAKLFPNTYDSPYLKFTSGTPDQPVRPLKVGVMLSG
GPAPGGHNVIWGLLHSLKKIHPDSSLIGFLNNGQGILNNHTIEITEEFMECFKNSGGFNCIGTGRTNIITEENKAVCLKT
VQALDLDGLVIIGGDGSNTATAILAEYFSQHHPKTCVVGVPKTIDGDLQHLFLDLTFGFDSATKFYSSIISNISRDTLSC
KAHYHVIKLMGRSASHIALECTLQTHPNITLIGEEIAEKNVPLNTIIHKVCSIIADRAAMGKYYGIILIPEGIIEFIPEI
NNLVKEIERIPEHADKFSSLSRESQKLLKSFPETIAKQLLNDRDAHGNVYVSKISVDKLLIHLVDNHLKKHFKNVPFNAI
SHFLGYEGRSCLPTKFDNTYSYALGYGAGVLTYNRCNGYLTVIESLINIVDKWRLRAMPIVKMFTTKKKSDGTIKPLIKK
SLIDISSPAFVKFKLYRKIWALEDSYRVLGPLQIDTPPNSHSDHFPPLTLLLNHNEWQKRCSICMEIPDCDY
>Mature_552_residues
MQSNSNDLNDVVIPGTPPLPRELQKFPSLIPTNDLRFSPKFDADVAKLFPNTYDSPYLKFTSGTPDQPVRPLKVGVMLSG
GPAPGGHNVIWGLLHSLKKIHPDSSLIGFLNNGQGILNNHTIEITEEFMECFKNSGGFNCIGTGRTNIITEENKAVCLKT
VQALDLDGLVIIGGDGSNTATAILAEYFSQHHPKTCVVGVPKTIDGDLQHLFLDLTFGFDSATKFYSSIISNISRDTLSC
KAHYHVIKLMGRSASHIALECTLQTHPNITLIGEEIAEKNVPLNTIIHKVCSIIADRAAMGKYYGIILIPEGIIEFIPEI
NNLVKEIERIPEHADKFSSLSRESQKLLKSFPETIAKQLLNDRDAHGNVYVSKISVDKLLIHLVDNHLKKHFKNVPFNAI
SHFLGYEGRSCLPTKFDNTYSYALGYGAGVLTYNRCNGYLTVIESLINIVDKWRLRAMPIVKMFTTKKKSDGTIKPLIKK
SLIDISSPAFVKFKLYRKIWALEDSYRVLGPLQIDTPPNSHSDHFPPLTLLLNHNEWQKRCSICMEIPDCDY

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Escherichia coli, GI1790350, Length=240, Percent_Identity=27.0833333333333, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 61555; Mature: 61555

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSNSNDLNDVVIPGTPPLPRELQKFPSLIPTNDLRFSPKFDADVAKLFPNTYDSPYLKF
CCCCCCCCCCEEECCCCCCCHHHHHCCCCCCCCCCEECCCCCCHHHHHCCCCCCCCEEEE
TSGTPDQPVRPLKVGVMLSGGPAPGGHNVIWGLLHSLKKIHPDSSLIGFLNNGQGILNNH
CCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCEEECCC
TIEITEEFMECFKNSGGFNCIGTGRTNIITEENKAVCLKTVQALDLDGLVIIGGDGSNTA
EEHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCHHHHHHHHCCCCCEEEEECCCCCHH
TAILAEYFSQHHPKTCVVGVPKTIDGDLQHLFLDLTFGFDSATKFYSSIISNISRDTLSC
HHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCHHHH
KAHYHVIKLMGRSASHIALECTLQTHPNITLIGEEIAEKNVPLNTIIHKVCSIIADRAAM
HHHHHHHHHHCCCCCEEEEEEEEECCCCEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHH
GKYYGIILIPEGIIEFIPEINNLVKEIERIPEHADKFSSLSRESQKLLKSFPETIAKQLL
CCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NDRDAHGNVYVSKISVDKLLIHLVDNHLKKHFKNVPFNAISHFLGYEGRSCLPTKFDNTY
CCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCE
SYALGYGAGVLTYNRCNGYLTVIESLINIVDKWRLRAMPIVKMFTTKKKSDGTIKPLIKK
EEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHH
SLIDISSPAFVKFKLYRKIWALEDSYRVLGPLQIDTPPNSHSDHFPPLTLLLNHNEWQKR
HHHCCCCCCEEEHHHHHHHHHCCCCCEEECCEEECCCCCCCCCCCCCEEEEECCCHHHHH
CSICMEIPDCDY
HHHEECCCCCCC
>Mature Secondary Structure
MQSNSNDLNDVVIPGTPPLPRELQKFPSLIPTNDLRFSPKFDADVAKLFPNTYDSPYLKF
CCCCCCCCCCEEECCCCCCCHHHHHCCCCCCCCCCEECCCCCCHHHHHCCCCCCCCEEEE
TSGTPDQPVRPLKVGVMLSGGPAPGGHNVIWGLLHSLKKIHPDSSLIGFLNNGQGILNNH
CCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCEEECCC
TIEITEEFMECFKNSGGFNCIGTGRTNIITEENKAVCLKTVQALDLDGLVIIGGDGSNTA
EEHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCHHHHHHHHCCCCCEEEEECCCCCHH
TAILAEYFSQHHPKTCVVGVPKTIDGDLQHLFLDLTFGFDSATKFYSSIISNISRDTLSC
HHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCHHHH
KAHYHVIKLMGRSASHIALECTLQTHPNITLIGEEIAEKNVPLNTIIHKVCSIIADRAAM
HHHHHHHHHHCCCCCEEEEEEEEECCCCEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHH
GKYYGIILIPEGIIEFIPEINNLVKEIERIPEHADKFSSLSRESQKLLKSFPETIAKQLL
CCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NDRDAHGNVYVSKISVDKLLIHLVDNHLKKHFKNVPFNAISHFLGYEGRSCLPTKFDNTY
CCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCE
SYALGYGAGVLTYNRCNGYLTVIESLINIVDKWRLRAMPIVKMFTTKKKSDGTIKPLIKK
EEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHH
SLIDISSPAFVKFKLYRKIWALEDSYRVLGPLQIDTPPNSHSDHFPPLTLLLNHNEWQKR
HHHCCCCCCEEEHHHHHHHHHCCCCCEEECCEEECCCCCCCCCCCCCEEEEECCCHHHHH
CSICMEIPDCDY
HHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]