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The map label for this gene is tgt

Identifier: 62185176

GI number: 62185176

Start: 635042

End: 636160

Strand: Direct

Name: tgt

Synonym: CAB559

Alternate gene names: 62185176

Gene position: 635042-636160 (Clockwise)

Preceding gene: 62185175

Following gene: 62185177

Centisome position: 55.49

GC content: 42.45

Gene sequence:

>1119_bases
GTGGCATTAAAATTTCATGTCCTCCATCAATCTAAAAAATCTCGAGCACGTGTCGGCAGAATAGAAACTGCTCATGGCAT
TATCGATACCCCAGCTTTTGTTCCTGTAGCGACAAATGGCGCTCTGAAAGGTGTTGTAGACCACAGCAATATCCAATTAA
TGTTTTGCAATACCTACCACCTTCTGGTCCACCCTGGCACAGAAGCTATCGCCGCTATGGGAGGCCTGCATAAGTTTATT
CATAGAAATTCCCCTATAATCACAGATTCTGGAGGATTCCAAATCTTTAGCCTGGCTTATGGCTCCGTTGCCGAAGAAAT
CAAAAGCTGTGGGAAAAAAAAAGGCTCGTCATCCATCTTAGAAGTTACTGATGAGGGAGTTTGGTTTAAATCTTACCGAG
ATGGTAGTAAGCTCTTCTTATCCCCAGAAGTTTCTGTACAAGCACAAAAAGACCTGGGTGCCGATATCATCATTCCTCTA
GATGAGCTTTTACCCTTTCACTCCGACACAACATATTTCCTATCTTCCTGTGCACGAACCTACGTATGGGAAAAACGCTC
CTTAGATTACCATAAAAAAGACCCAAGACATCAATCTATGTATGGTGTGATTCACGGCGGTATAGATCCCAAACAAAGAA
AAATTGGTTGCGCCTTTGTTGAAGATCACCCTTTTGACGGATTTGCCATTGGGGGAAGCTTAGGAAGAAATCTCAACGAA
ATGGTCCCTATTGTTGATATAACAACTTCTTACTTATCCAAAGACCGTCCCGTACATCTTTTAGGCATAGGAGACCTTCC
CTCTATACAGGCAACTATTGGTTTTGGTATAGATTCTTTCGATAGTTCCTATCCAACTAAAGCCGCACGACACGGATTAA
TCCTGTCTTCTCAAGGCCCTATCAAAATCGCCAACCAGGCGTATACTAACGATCTCTCTCCTATAGATCCAGAGTGCACG
TGCCTCACATGCACCTCTAAGATCTCTAGAGCTTATCTGCGACATCTTTTCAAAGTTCACGAGCCTAATGCAAGCATTTG
GGCCTCCATCCATAATCTTCACTACATGCAAGAATTTATGAAAAATATCCGCGAACAAATTTTACATGATAGGATTTAG

Upstream 100 bases:

>100_bases
ATTTACAGGAAAATATGCTCCCCTATAGGCTTATTCTACTGTTTTCGAATGAGATGAAAGCAGAAGTCTTTTTTCGGGGC
AGCAGTTTCGGGTTCCTATC

Downstream 100 bases:

>100_bases
TTTTTTGTTTTTCTTTTCGGATTATTGTACTTAACTCGAAGTATTTAAGATGAGTTTACATAAAAAACAAATAAGAGAAA
TTTGATTTTTATGTAAACTC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MALKFHVLHQSKKSRARVGRIETAHGIIDTPAFVPVATNGALKGVVDHSNIQLMFCNTYHLLVHPGTEAIAAMGGLHKFI
HRNSPIITDSGGFQIFSLAYGSVAEEIKSCGKKKGSSSILEVTDEGVWFKSYRDGSKLFLSPEVSVQAQKDLGADIIIPL
DELLPFHSDTTYFLSSCARTYVWEKRSLDYHKKDPRHQSMYGVIHGGIDPKQRKIGCAFVEDHPFDGFAIGGSLGRNLNE
MVPIVDITTSYLSKDRPVHLLGIGDLPSIQATIGFGIDSFDSSYPTKAARHGLILSSQGPIKIANQAYTNDLSPIDPECT
CLTCTSKISRAYLRHLFKVHEPNASIWASIHNLHYMQEFMKNIREQILHDRI

Sequences:

>Translated_372_residues
MALKFHVLHQSKKSRARVGRIETAHGIIDTPAFVPVATNGALKGVVDHSNIQLMFCNTYHLLVHPGTEAIAAMGGLHKFI
HRNSPIITDSGGFQIFSLAYGSVAEEIKSCGKKKGSSSILEVTDEGVWFKSYRDGSKLFLSPEVSVQAQKDLGADIIIPL
DELLPFHSDTTYFLSSCARTYVWEKRSLDYHKKDPRHQSMYGVIHGGIDPKQRKIGCAFVEDHPFDGFAIGGSLGRNLNE
MVPIVDITTSYLSKDRPVHLLGIGDLPSIQATIGFGIDSFDSSYPTKAARHGLILSSQGPIKIANQAYTNDLSPIDPECT
CLTCTSKISRAYLRHLFKVHEPNASIWASIHNLHYMQEFMKNIREQILHDRI
>Mature_371_residues
ALKFHVLHQSKKSRARVGRIETAHGIIDTPAFVPVATNGALKGVVDHSNIQLMFCNTYHLLVHPGTEAIAAMGGLHKFIH
RNSPIITDSGGFQIFSLAYGSVAEEIKSCGKKKGSSSILEVTDEGVWFKSYRDGSKLFLSPEVSVQAQKDLGADIIIPLD
ELLPFHSDTTYFLSSCARTYVWEKRSLDYHKKDPRHQSMYGVIHGGIDPKQRKIGCAFVEDHPFDGFAIGGSLGRNLNEM
VPIVDITTSYLSKDRPVHLLGIGDLPSIQATIGFGIDSFDSSYPTKAARHGLILSSQGPIKIANQAYTNDLSPIDPECTC
LTCTSKISRAYLRHLFKVHEPNASIWASIHNLHYMQEFMKNIREQILHDRI

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=372, Percent_Identity=34.6774193548387, Blast_Score=226, Evalue=3e-59,
Organism=Escherichia coli, GI1786607, Length=370, Percent_Identity=35.1351351351351, Blast_Score=236, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17544680, Length=371, Percent_Identity=31.8059299191375, Blast_Score=185, Evalue=4e-47,
Organism=Drosophila melanogaster, GI19920510, Length=373, Percent_Identity=34.3163538873995, Blast_Score=230, Evalue=1e-60,
Organism=Drosophila melanogaster, GI21355311, Length=202, Percent_Identity=28.7128712871287, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_CHLAB (Q5L5T1)

Other databases:

- EMBL:   CR848038
- RefSeq:   YP_219961.1
- HSSP:   P28720
- ProteinModelPortal:   Q5L5T1
- SMR:   Q5L5T1
- GeneID:   3337719
- GenomeReviews:   CR848038_GR
- KEGG:   cab:CAB559
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- ProtClustDB:   PRK01008
- BioCyc:   CABO218497:CAB559-MONOMER
- BRENDA:   2.4.2.29
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 41257; Mature: 41125

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: NA

Important sites: ACT_SITE 89-89 BINDING 90-90

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALKFHVLHQSKKSRARVGRIETAHGIIDTPAFVPVATNGALKGVVDHSNIQLMFCNTYH
CCEEEEEHHCCHHHHHHHCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCEEEEEEEEEE
LLVHPGTEAIAAMGGLHKFIHRNSPIITDSGGFQIFSLAYGSVAEEIKSCGKKKGSSSIL
EEEECCHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEHHHHHHHHHHHHHCCCCCCCEE
EVTDEGVWFKSYRDGSKLFLSPEVSVQAQKDLGADIIIPLDELLPFHSDTTYFLSSCART
EEECCCEEEEECCCCCEEEECCCCCEEECCCCCCEEEEEHHHHCCCCCCHHHHHHHHHHH
YVWEKRSLDYHKKDPRHQSMYGVIHGGIDPKQRKIGCAFVEDHPFDGFAIGGSLGRNLNE
HHHHCCCCCCCCCCCCHHHEEEEEECCCCCCHHEEEEEEEECCCCCCEEECCHHCCCHHH
MVPIVDITTSYLSKDRPVHLLGIGDLPSIQATIGFGIDSFDSSYPTKAARHGLILSSQGP
CCCCEEEHHHHHCCCCCEEEEECCCCCCCEEEEECCCCCCCCCCCCHHHHCCEEEECCCC
IKIANQAYTNDLSPIDPECTCLTCTSKISRAYLRHLFKVHEPNASIWASIHNLHYMQEFM
EEECCCHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
KNIREQILHDRI
HHHHHHHHHCCC
>Mature Secondary Structure 
ALKFHVLHQSKKSRARVGRIETAHGIIDTPAFVPVATNGALKGVVDHSNIQLMFCNTYH
CEEEEEHHCCHHHHHHHCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCEEEEEEEEEE
LLVHPGTEAIAAMGGLHKFIHRNSPIITDSGGFQIFSLAYGSVAEEIKSCGKKKGSSSIL
EEEECCHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEHHHHHHHHHHHHHCCCCCCCEE
EVTDEGVWFKSYRDGSKLFLSPEVSVQAQKDLGADIIIPLDELLPFHSDTTYFLSSCART
EEECCCEEEEECCCCCEEEECCCCCEEECCCCCCEEEEEHHHHCCCCCCHHHHHHHHHHH
YVWEKRSLDYHKKDPRHQSMYGVIHGGIDPKQRKIGCAFVEDHPFDGFAIGGSLGRNLNE
HHHHCCCCCCCCCCCCHHHEEEEEECCCCCCHHEEEEEEEECCCCCCEEECCHHCCCHHH
MVPIVDITTSYLSKDRPVHLLGIGDLPSIQATIGFGIDSFDSSYPTKAARHGLILSSQGP
CCCCEEEHHHHHCCCCCEEEEECCCCCCCEEEEECCCCCCCCCCCCHHHHCCEEEECCCC
IKIANQAYTNDLSPIDPECTCLTCTSKISRAYLRHLFKVHEPNASIWASIHNLHYMQEFM
EEECCCHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
KNIREQILHDRI
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA