Click here to switch to the map view.

The map label for this gene is hemF [H]

Identifier: 59713741

GI number: 59713741

Start: 642482

End: 643447

Strand: Reverse

Name: hemF [H]

Synonym: VF_A0558

Alternate gene names: 59713741

Gene position: 643447-642482 (Counterclockwise)

Preceding gene: 59713749

Following gene: 59713739

Centisome position: 48.37

GC content: 36.85

Gene sequence:

>966_bases
ATGCGGATTTATGCTAAAAACCCCGATGCTCAAAGTGCATTACAACTGCTCAGAAGTATTCAATCTTATTTTAAATTTGA
GCTTTTAAAAGCAAGCAGTCTAACATCACAATCAACCGAATTTAAAGCCGTAGAGTGGCTAAGAGATAATGGAAGTCATG
GTGGTGGCGAGCGCTTTGAAGCCATTGATGGTGGAATATTTAATCGAGCATCCATCAATGTTTCACAAATTCAATACGAA
GACGATGCTAGCAAACATTATAACAGCGCTACTGCTCTTTCTACCATTATCCACCCTAATAACCCCCTCGCACCATCGAT
TCATATGCATTTTAGTTTAACGGAATTAAAAGATGGTACTAGTTATTGGCGAATCATGGCTGATTTGAACCCTTCTCACT
TTGATAAAGAAGACAAAGAATACTTTGATCAAACAGTAAAAGTTGCAGCAAAATCGTACTATTTAGAAGGAACTAAGGCA
GGTAATCACTATTTCGATATCCCAGCATTAAAAAAACGCAGAGGTGTTAGTCATTTTTATTTGGAAGGATTTAATCCGGA
AGGCGAAAATGGCGAATTATTCACAAAACAATTTGCAACAAAAGTGATTGATACTTATGTGCATATTCTTAGCTCACACT
TACTGAAAACAAATGAAATAACCAAAGAACAAAAACAAACTCAATTGAATTACCACACACTTTATTTCTATCAGGTATTG
ACCTTAGACAAAGGAACCACGGCAGGCTTATTAGTTCACGATCAAAATGATGTAGGAACACTGGGTTCTTTGCCTTCACA
TATCAATCGTCCATTACTTGAAGACTGGCAAGCTTTAACACCTCAACCAACAGACCGATTAGTTTCAGAGTTATTAGCAG
CGCTTCCTTGTGAAAATACGTGTTCAATAACAAAAGAAGTTAAGGCTAACCTAGCTCAAGCCATTCGCGCATTTTACAAA
AAATAA

Upstream 100 bases:

>100_bases
CTTTCCATTTCATAGGTTGCTATATACAAAGGCTTTATTTTACACTCAATTAATAAGTGTTTAACCTTTTCTATATTACT
TGGTTTATTAAGGTCTTTTT

Downstream 100 bases:

>100_bases
CATGCATAGTGCCACCACGCATCATATCGTGGTGGTAAGCATTCTTATTATAAGGTTGGTTTAATCCAAACCTTCTCAAA
ATCAAACCAGCCTAATCCAT

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MRIYAKNPDAQSALQLLRSIQSYFKFELLKASSLTSQSTEFKAVEWLRDNGSHGGGERFEAIDGGIFNRASINVSQIQYE
DDASKHYNSATALSTIIHPNNPLAPSIHMHFSLTELKDGTSYWRIMADLNPSHFDKEDKEYFDQTVKVAAKSYYLEGTKA
GNHYFDIPALKKRRGVSHFYLEGFNPEGENGELFTKQFATKVIDTYVHILSSHLLKTNEITKEQKQTQLNYHTLYFYQVL
TLDKGTTAGLLVHDQNDVGTLGSLPSHINRPLLEDWQALTPQPTDRLVSELLAALPCENTCSITKEVKANLAQAIRAFYK
K

Sequences:

>Translated_321_residues
MRIYAKNPDAQSALQLLRSIQSYFKFELLKASSLTSQSTEFKAVEWLRDNGSHGGGERFEAIDGGIFNRASINVSQIQYE
DDASKHYNSATALSTIIHPNNPLAPSIHMHFSLTELKDGTSYWRIMADLNPSHFDKEDKEYFDQTVKVAAKSYYLEGTKA
GNHYFDIPALKKRRGVSHFYLEGFNPEGENGELFTKQFATKVIDTYVHILSSHLLKTNEITKEQKQTQLNYHTLYFYQVL
TLDKGTTAGLLVHDQNDVGTLGSLPSHINRPLLEDWQALTPQPTDRLVSELLAALPCENTCSITKEVKANLAQAIRAFYK
K
>Mature_321_residues
MRIYAKNPDAQSALQLLRSIQSYFKFELLKASSLTSQSTEFKAVEWLRDNGSHGGGERFEAIDGGIFNRASINVSQIQYE
DDASKHYNSATALSTIIHPNNPLAPSIHMHFSLTELKDGTSYWRIMADLNPSHFDKEDKEYFDQTVKVAAKSYYLEGTKA
GNHYFDIPALKKRRGVSHFYLEGFNPEGENGELFTKQFATKVIDTYVHILSSHLLKTNEITKEQKQTQLNYHTLYFYQVL
TLDKGTTAGLLVHDQNDVGTLGSLPSHINRPLLEDWQALTPQPTDRLVSELLAALPCENTCSITKEVKANLAQAIRAFYK
K

Specific function: Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III [H]

COG id: COG0408

COG function: function code H; Coproporphyrinogen III oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI41393599, Length=265, Percent_Identity=26.0377358490566, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1788777, Length=262, Percent_Identity=24.0458015267176, Blast_Score=65, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6320249, Length=272, Percent_Identity=28.3088235294118, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI17864388, Length=277, Percent_Identity=28.8808664259928, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001260
- InterPro:   IPR018375 [H]

Pfam domain/function: PF01218 Coprogen_oxidas [H]

EC number: =1.3.3.3 [H]

Molecular weight: Translated: 36298; Mature: 36298

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIYAKNPDAQSALQLLRSIQSYFKFELLKASSLTSQSTEFKAVEWLRDNGSHGGGERFE
CEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHH
AIDGGIFNRASINVSQIQYEDDASKHYNSATALSTIIHPNNPLAPSIHMHFSLTELKDGT
HHCCCCCCCCCCCHHHEEECCCHHHHCCHHHHHHEEECCCCCCCCEEEEEEEEHHHCCCC
SYWRIMADLNPSHFDKEDKEYFDQTVKVAAKSYYLEGTKAGNHYFDIPALKKRRGVSHFY
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCEEECCCHHHHHCCCCEEE
LEGFNPEGENGELFTKQFATKVIDTYVHILSSHLLKTNEITKEQKQTQLNYHTLYFYQVL
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEE
TLDKGTTAGLLVHDQNDVGTLGSLPSHINRPLLEDWQALTPQPTDRLVSELLAALPCENT
EECCCCCEEEEEECCCCCCCCCCCHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
CSITKEVKANLAQAIRAFYKK
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRIYAKNPDAQSALQLLRSIQSYFKFELLKASSLTSQSTEFKAVEWLRDNGSHGGGERFE
CEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHH
AIDGGIFNRASINVSQIQYEDDASKHYNSATALSTIIHPNNPLAPSIHMHFSLTELKDGT
HHCCCCCCCCCCCHHHEEECCCHHHHCCHHHHHHEEECCCCCCCCEEEEEEEEHHHCCCC
SYWRIMADLNPSHFDKEDKEYFDQTVKVAAKSYYLEGTKAGNHYFDIPALKKRRGVSHFY
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCEEECCCHHHHHCCCCEEE
LEGFNPEGENGELFTKQFATKVIDTYVHILSSHLLKTNEITKEQKQTQLNYHTLYFYQVL
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEE
TLDKGTTAGLLVHDQNDVGTLGSLPSHINRPLLEDWQALTPQPTDRLVSELLAALPCENT
EECCCCCEEEEEECCCCCCCCCCCHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
CSITKEVKANLAQAIRAFYKK
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA