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The map label for this gene is gloA [H]

Identifier: 59711535

GI number: 59711535

Start: 1023194

End: 1023610

Strand: Reverse

Name: gloA [H]

Synonym: VF_0928

Alternate gene names: 59711535

Gene position: 1023610-1023194 (Counterclockwise)

Preceding gene: 59711536

Following gene: 59711534

Centisome position: 35.33

GC content: 40.29

Gene sequence:

>417_bases
ATGGCTAATGGACGCATTTTACATACTATGATCCGCGTGGGTAACTTAGATAAATCCATCGAATTTTATACCAAAGTAAT
GGGAATGGATCTTCTTCGTCAAAATACGAATGAAGAATACAAATACACACTGGCTTTTCTTGGCTATGGTGATGAATCCC
AAGGTGCCGTAATTGAACTTACCTATAACTGGGGCACAGAAGAATACGACATGGGTACTGCTTTTGGTCACATTGCTATT
GGTGTTGATGATATCTATGCAACTTGTGACGCAATTAAAGCTGCAGGAGGCAATGTTACGCGCGAAGCAGGTCCTGTAAA
AGGTGGCTCAACACATATTGCTTTTGTAAAAGATCCTGATGGTTACATGATTGAGTTAATTCAAAACAGCAGTGCATCAG
CGGGATTAGAAGGTTAA

Upstream 100 bases:

>100_bases
CGTTATACTTGTATAGCTCGCAAACCTCGTTGTGGTAGCTGTATGATCGAAGATTTATGTGAATTCAAAGAAAAAACAGA
AGATTAATAGGAGCAAAACA

Downstream 100 bases:

>100_bases
AAAATACCTTATAATCCACTTTACAGTAAAAAACCTGCGATCTAGGCAGGTTTTTTTATACATTAAACTTTAATTGATAA
TAATTCTCAGTTAGATTTAT

Product: glyoxalase I, Ni-dependent

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 138; Mature: 137

Protein sequence:

>138_residues
MANGRILHTMIRVGNLDKSIEFYTKVMGMDLLRQNTNEEYKYTLAFLGYGDESQGAVIELTYNWGTEEYDMGTAFGHIAI
GVDDIYATCDAIKAAGGNVTREAGPVKGGSTHIAFVKDPDGYMIELIQNSSASAGLEG

Sequences:

>Translated_138_residues
MANGRILHTMIRVGNLDKSIEFYTKVMGMDLLRQNTNEEYKYTLAFLGYGDESQGAVIELTYNWGTEEYDMGTAFGHIAI
GVDDIYATCDAIKAAGGNVTREAGPVKGGSTHIAFVKDPDGYMIELIQNSSASAGLEG
>Mature_137_residues
ANGRILHTMIRVGNLDKSIEFYTKVMGMDLLRQNTNEEYKYTLAFLGYGDESQGAVIELTYNWGTEEYDMGTAFGHIAIG
VDDIYATCDAIKAAGGNVTREAGPVKGGSTHIAFVKDPDGYMIELIQNSSASAGLEG

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=146, Percent_Identity=35.6164383561644, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI1787940, Length=132, Percent_Identity=65.1515151515152, Blast_Score=189, Evalue=6e-50,
Organism=Caenorhabditis elegans, GI17552228, Length=134, Percent_Identity=29.1044776119403, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323639, Length=138, Percent_Identity=42.7536231884058, Blast_Score=102, Evalue=2e-23,
Organism=Drosophila melanogaster, GI20129743, Length=148, Percent_Identity=38.5135135135135, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19920404, Length=146, Percent_Identity=31.5068493150685, Blast_Score=73, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14940; Mature: 14809

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS00934 GLYOXALASE_I_1 ; PS00935 GLYOXALASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANGRILHTMIRVGNLDKSIEFYTKVMGMDLLRQNTNEEYKYTLAFLGYGDESQGAVIEL
CCCCCEEEEEEHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEEE
TYNWGTEEYDMGTAFGHIAIGVDDIYATCDAIKAAGGNVTREAGPVKGGSTHIAFVKDPD
EECCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCC
GYMIELIQNSSASAGLEG
CEEEEEEECCCCCCCCCC
>Mature Secondary Structure 
ANGRILHTMIRVGNLDKSIEFYTKVMGMDLLRQNTNEEYKYTLAFLGYGDESQGAVIEL
CCCCEEEEEEHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEEE
TYNWGTEEYDMGTAFGHIAIGVDDIYATCDAIKAAGGNVTREAGPVKGGSTHIAFVKDPD
EECCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCC
GYMIELIQNSSASAGLEG
CEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739; 8021208 [H]