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The map label for this gene is cobA [H]

Identifier: 59711380

GI number: 59711380

Start: 842477

End: 843253

Strand: Reverse

Name: cobA [H]

Synonym: VF_0773

Alternate gene names: 59711380

Gene position: 843253-842477 (Counterclockwise)

Preceding gene: 59711381

Following gene: 59711373

Centisome position: 29.1

GC content: 39.9

Gene sequence:

>777_bases
ATGCCAAGTATTCAAGCTCAGTCTGAGCAGAAAAAACAAAACGGACTTGTTTCTCTTGTTGGCGCAGGCCCTGGTGATCC
CGAACTATTAACCATGAAGGGCTATCGATTAATCAAAGAAGCGGATGTTTTGGTTTATGATCGCCTAGTCTCAAAAGAAA
TTTTAGCGCTTGCAAATGAAAATGCTGAAATGATTTATGTTGGAAAAAAACTGGATTTCCACTTCGTTCCTCAAGATAAA
ATCAATCAAATTCTAGTCGATAAAGCAAAGGAAGGTAAGCACGTTGTTCGCCTTAAAGGTGGGGATTCATTTATCTTTGG
GCGTGGCGGAGAAGAGCTTGAAACGTTAGCTGAAAATGGCGTTCACTTTGAAGTTATTCCGGGGATCACAGCTGCGGCGG
GGGCAACCTCATACGCAGGTATCCCATTAACTCACCGAGATCATGCCCAAAGTGTTCAATTTATCACAGCTCATGTACAA
AAAGATGGTGCTGATATTGAATGGGATGCATTAGTCCGCTCTAATAATACCTTAGTATTTTATATGGGATTAAAGCAATC
TCCTCATATATCTGAACAGTTAATAAAACATGGATTAGCTGCTGATACACCATGTGCAATTATTGAGCGAGGCACAACAA
ATAAACAAAAAGTATTTACTGGCGATATTTCAGAACTTGCGCAGTTAGCAGAAAAAGCCATCAGTCCATCGCTGATCGTA
GTAGGTACCGTAACAACACTTCATCATAAATTAGATTGGTTTAAACCAAACGAATAA

Upstream 100 bases:

>100_bases
ACTCATGCGATTAAAATCACTCTTAATAATTGATTAAGGGTGATTTTTTTTATCGACTCTTACATACTAAGGTGATTAAA
CCTTATTCTGGAATTCCATT

Downstream 100 bases:

>100_bases
TTTTTTACCATTCAGATAATAAAAAAGCCCAAGAAAGTATTCTCTCTTGGGCTTTTTTATTTATTCTTTAAAACTCGATT
ATTTAATATCGATGTGCTCG

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MPSIQAQSEQKKQNGLVSLVGAGPGDPELLTMKGYRLIKEADVLVYDRLVSKEILALANENAEMIYVGKKLDFHFVPQDK
INQILVDKAKEGKHVVRLKGGDSFIFGRGGEELETLAENGVHFEVIPGITAAAGATSYAGIPLTHRDHAQSVQFITAHVQ
KDGADIEWDALVRSNNTLVFYMGLKQSPHISEQLIKHGLAADTPCAIIERGTTNKQKVFTGDISELAQLAEKAISPSLIV
VGTVTTLHHKLDWFKPNE

Sequences:

>Translated_258_residues
MPSIQAQSEQKKQNGLVSLVGAGPGDPELLTMKGYRLIKEADVLVYDRLVSKEILALANENAEMIYVGKKLDFHFVPQDK
INQILVDKAKEGKHVVRLKGGDSFIFGRGGEELETLAENGVHFEVIPGITAAAGATSYAGIPLTHRDHAQSVQFITAHVQ
KDGADIEWDALVRSNNTLVFYMGLKQSPHISEQLIKHGLAADTPCAIIERGTTNKQKVFTGDISELAQLAEKAISPSLIV
VGTVTTLHHKLDWFKPNE
>Mature_257_residues
PSIQAQSEQKKQNGLVSLVGAGPGDPELLTMKGYRLIKEADVLVYDRLVSKEILALANENAEMIYVGKKLDFHFVPQDKI
NQILVDKAKEGKHVVRLKGGDSFIFGRGGEELETLAENGVHFEVIPGITAAAGATSYAGIPLTHRDHAQSVQFITAHVQK
DGADIEWDALVRSNNTLVFYMGLKQSPHISEQLIKHGLAADTPCAIIERGTTNKQKVFTGDISELAQLAEKAISPSLIVV
GTVTTLHHKLDWFKPNE

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=250, Percent_Identity=54, Blast_Score=285, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6322922, Length=242, Percent_Identity=36.3636363636364, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 28186; Mature: 28054

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSIQAQSEQKKQNGLVSLVGAGPGDPELLTMKGYRLIKEADVLVYDRLVSKEILALANE
CCCCCCCHHHHHHCCEEEEEECCCCCCCEEEECCCEEEHHCCHHHHHHHHHHHHHHHCCC
NAEMIYVGKKLDFHFVPQDKINQILVDKAKEGKHVVRLKGGDSFIFGRGGEELETLAENG
CCEEEEEECCCEEEECCHHHHHHHHHHCCCCCCEEEEEECCCEEEECCCCHHHHHHHHCC
VHFEVIPGITAAAGATSYAGIPLTHRDHAQSVQFITAHVQKDGADIEWDALVRSNNTLVF
CEEEEECCCCHHCCCCCCCCCCCCCCCHHHHHHHHEEEECCCCCCCEEEEEEECCCEEEE
YMGLKQSPHISEQLIKHGLAADTPCAIIERGTTNKQKVFTGDISELAQLAEKAISPSLIV
EECCCCCCCHHHHHHHCCCCCCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHCCCCEEE
VGTVTTLHHKLDWFKPNE
EEEHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PSIQAQSEQKKQNGLVSLVGAGPGDPELLTMKGYRLIKEADVLVYDRLVSKEILALANE
CCCCCCHHHHHHCCEEEEEECCCCCCCEEEECCCEEEHHCCHHHHHHHHHHHHHHHCCC
NAEMIYVGKKLDFHFVPQDKINQILVDKAKEGKHVVRLKGGDSFIFGRGGEELETLAENG
CCEEEEEECCCEEEECCHHHHHHHHHHCCCCCCEEEEEECCCEEEECCCCHHHHHHHHCC
VHFEVIPGITAAAGATSYAGIPLTHRDHAQSVQFITAHVQKDGADIEWDALVRSNNTLVF
CEEEEECCCCHHCCCCCCCCCCCCCCCHHHHHHHHEEEECCCCCCCEEEEEEECCCEEEE
YMGLKQSPHISEQLIKHGLAADTPCAIIERGTTNKQKVFTGDISELAQLAEKAISPSLIV
EECCCCCCCHHHHHHHCCCCCCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHCCCCEEE
VGTVTTLHHKLDWFKPNE
EEEHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA