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The map label for this gene is mrdA [H]

Identifier: 59711355

GI number: 59711355

Start: 815431

End: 817311

Strand: Reverse

Name: mrdA [H]

Synonym: VF_0748

Alternate gene names: 59711355

Gene position: 817311-815431 (Counterclockwise)

Preceding gene: 59711356

Following gene: 59711354

Centisome position: 28.21

GC content: 39.29

Gene sequence:

>1881_bases
TTGATTAAGCGCAAACGCACACAGATCCGTGACTATCACGCTGAAACGAGATTATTTGCATCACGTGCAATTATCTCGTT
TTCAGGGATCATTGTACTTATTGGTCTGCTTTTGACTAACTTGTACAACATTCAAATCAATGAATTTCAAGATTATAAAA
CACGCTCAAATGATAACCGTATTAAGATAGTCCCTATCGCACCAAACCGAGGATTAATCTTTGATAGAAGCGGAAATCCA
TTAGCGGAAAACCGCCCTGTTTTTAATTTAGAAGTCACCCCTGACAAAGTGTCAGATCTTTCTCTTTTGTTTACAGAATT
ACAAACTATCTTGCCAATAACACAAGATAATATTGATGACTTTCAGAAAGAACGTCGCCGAACTCGTCGTTTTAAATCCG
TCCCTCTTCTTACCCAACTAACGCAAGAACAGGTAGCGATATTTTCTGTGAATCAGCATAAATTCCAAGGTGTTGAAATA
AAAGCCTACTTAAAGCGCTTTTATCCTTACGGAAAAACACTCACTCATGTACTTGGTTATGTAGCTAAAATTAACGATCG
TGATTTGGCTCGTTTAGCACGTGAAGAAAAAGACGCAAATTATCAAGCAACTCGTGATATTGGTAAATTAGGTATTGAAC
GCTTTTATGAAGATATCCTTCATGGAACCGCCGGCTATCAAGAAGTTGAAGTTAATAGCCGAGGCCGAGTAATTCGAACG
TTAAAATACGTACCACCAATCCCTGGTAAAGACATCGTATTAAACCTTGATATAAAACTTCAAAAGTTTGTTGAAAAACA
ACTGGATGGACGCAGAGGTTCTGCCATTGTACTTGACCCTAAAACCAGTGCAGTTTTAGCTATGGTTTCAAGCCCAAGCT
ATGATCCAAACTTATTTGTACATGGTATTTCAAGTAAAAATTACAGCGCTCTATTAAATGATCCTGATCGTCCATTAGTT
AACAGAAGCACATTAGGTATCTATCCGCCAGCATCAACCGTAAAACCATTTATTGCTGTTGCAGCATTAAAAGAGAAAAT
TGTTACGACAGAAACAACTCGTGATGACCCGGGTTATTGGCGCATACCTAACTCTAAGACTAAACCATTTAGAGACTGGA
AGCGCTGGGGTCACGGTACAGTTAACTTAAAACAATCTATTGAAGAGTCTGTTGATACCTTCTTCTATCAAATCGCCTAT
GATATGGGCATTGATAGACTATCAACGTGGATGATGAAGTTTGGTTTTGGTGATTACACCGGTATTGATATTCGTGAAGA
AAGCAAAGCGAATATGCCTACTCGAGAATGGAAAGTTGCCCGTTTCCGTACCCCATGGTATCAAGGGGATACTATCCCTG
TAGGTATTGGTCAAGGCTATTGGACAGCAACGCCAATGCAGCTTGCAAAAGCAACTGCTGTATTAACCAACCATGGTAAA
GTTCAAGCACCACATTTATTAAAAGGTATTATTGAAAATAATAAAGAAGTGCCTTATGAACCAGAGCCTTACCCACCAAT
TGAAGGGGTAAAAGACAGTTACTGGCAATTAGCTATTGATGGCATGATTCTAGTAAATAATGGACGTAAAGGTACAGCAA
GAAGAGCTTTTGCAGGCACACCTTATGTGAGTGCAGGTAAATCAGGTACATCGCAAGTATTCGGTCTAAAAGAAGATGAA
GAATATAATGCTGATGAAATTGCAGAGCATTTGCGTGACCACGCTTTATTTACCGGTTTTGCACCAGCAAAAGATCCAAA
AGTTATTATTTCTCTCGTATTAGAGAATGGTGGTGGTGGCTCTAGCCAAGGTGGTCCAATAGCTCGTCACGTCTTCGATT
ATGTATTACTACCGAAAACGGAAGAGGCAGCTAAGAAGTAA

Upstream 100 bases:

>100_bases
ACTAACCCTCCCTCACCCTCTGGTTCGAGTTGTCATGGCTGAAAGCCTATATCGAGCATGGAGTATTACGGCAAATCATC
CTTACCATAGAGAATAAGAG

Downstream 100 bases:

>100_bases
TGAGAGTTGATATTGCATCAGGAAAGAATCGCGACTTTTTTCATCGTATCCATTTAGATTTACCACTATTGCTAGGCATT
TTATTATTGATGGCATGTGC

Product: transpeptidase (penicillin-binding protein 2)

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 626; Mature: 626

Protein sequence:

>626_residues
MIKRKRTQIRDYHAETRLFASRAIISFSGIIVLIGLLLTNLYNIQINEFQDYKTRSNDNRIKIVPIAPNRGLIFDRSGNP
LAENRPVFNLEVTPDKVSDLSLLFTELQTILPITQDNIDDFQKERRRTRRFKSVPLLTQLTQEQVAIFSVNQHKFQGVEI
KAYLKRFYPYGKTLTHVLGYVAKINDRDLARLAREEKDANYQATRDIGKLGIERFYEDILHGTAGYQEVEVNSRGRVIRT
LKYVPPIPGKDIVLNLDIKLQKFVEKQLDGRRGSAIVLDPKTSAVLAMVSSPSYDPNLFVHGISSKNYSALLNDPDRPLV
NRSTLGIYPPASTVKPFIAVAALKEKIVTTETTRDDPGYWRIPNSKTKPFRDWKRWGHGTVNLKQSIEESVDTFFYQIAY
DMGIDRLSTWMMKFGFGDYTGIDIREESKANMPTREWKVARFRTPWYQGDTIPVGIGQGYWTATPMQLAKATAVLTNHGK
VQAPHLLKGIIENNKEVPYEPEPYPPIEGVKDSYWQLAIDGMILVNNGRKGTARRAFAGTPYVSAGKSGTSQVFGLKEDE
EYNADEIAEHLRDHALFTGFAPAKDPKVIISLVLENGGGGSSQGGPIARHVFDYVLLPKTEEAAKK

Sequences:

>Translated_626_residues
MIKRKRTQIRDYHAETRLFASRAIISFSGIIVLIGLLLTNLYNIQINEFQDYKTRSNDNRIKIVPIAPNRGLIFDRSGNP
LAENRPVFNLEVTPDKVSDLSLLFTELQTILPITQDNIDDFQKERRRTRRFKSVPLLTQLTQEQVAIFSVNQHKFQGVEI
KAYLKRFYPYGKTLTHVLGYVAKINDRDLARLAREEKDANYQATRDIGKLGIERFYEDILHGTAGYQEVEVNSRGRVIRT
LKYVPPIPGKDIVLNLDIKLQKFVEKQLDGRRGSAIVLDPKTSAVLAMVSSPSYDPNLFVHGISSKNYSALLNDPDRPLV
NRSTLGIYPPASTVKPFIAVAALKEKIVTTETTRDDPGYWRIPNSKTKPFRDWKRWGHGTVNLKQSIEESVDTFFYQIAY
DMGIDRLSTWMMKFGFGDYTGIDIREESKANMPTREWKVARFRTPWYQGDTIPVGIGQGYWTATPMQLAKATAVLTNHGK
VQAPHLLKGIIENNKEVPYEPEPYPPIEGVKDSYWQLAIDGMILVNNGRKGTARRAFAGTPYVSAGKSGTSQVFGLKEDE
EYNADEIAEHLRDHALFTGFAPAKDPKVIISLVLENGGGGSSQGGPIARHVFDYVLLPKTEEAAKK
>Mature_626_residues
MIKRKRTQIRDYHAETRLFASRAIISFSGIIVLIGLLLTNLYNIQINEFQDYKTRSNDNRIKIVPIAPNRGLIFDRSGNP
LAENRPVFNLEVTPDKVSDLSLLFTELQTILPITQDNIDDFQKERRRTRRFKSVPLLTQLTQEQVAIFSVNQHKFQGVEI
KAYLKRFYPYGKTLTHVLGYVAKINDRDLARLAREEKDANYQATRDIGKLGIERFYEDILHGTAGYQEVEVNSRGRVIRT
LKYVPPIPGKDIVLNLDIKLQKFVEKQLDGRRGSAIVLDPKTSAVLAMVSSPSYDPNLFVHGISSKNYSALLNDPDRPLV
NRSTLGIYPPASTVKPFIAVAALKEKIVTTETTRDDPGYWRIPNSKTKPFRDWKRWGHGTVNLKQSIEESVDTFFYQIAY
DMGIDRLSTWMMKFGFGDYTGIDIREESKANMPTREWKVARFRTPWYQGDTIPVGIGQGYWTATPMQLAKATAVLTNHGK
VQAPHLLKGIIENNKEVPYEPEPYPPIEGVKDSYWQLAIDGMILVNNGRKGTARRAFAGTPYVSAGKSGTSQVFGLKEDE
EYNADEIAEHLRDHALFTGFAPAKDPKVIISLVLENGGGGSSQGGPIARHVFDYVLLPKTEEAAKK

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=612, Percent_Identity=58.4967320261438, Blast_Score=772, Evalue=0.0,
Organism=Escherichia coli, GI1786272, Length=630, Percent_Identity=23.6507936507936, Blast_Score=120, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 70755; Mature: 70755

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRKRTQIRDYHAETRLFASRAIISFSGIIVLIGLLLTNLYNIQINEFQDYKTRSNDNR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHCCCCCCE
IKIVPIAPNRGLIFDRSGNPLAENRPVFNLEVTPDKVSDLSLLFTELQTILPITQDNIDD
EEEEEECCCCCEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHH
FQKERRRTRRFKSVPLLTQLTQEQVAIFSVNQHKFQGVEIKAYLKRFYPYGKTLTHVLGY
HHHHHHHHHHHHCCCHHHHHHHHCEEEEEECCHHCCCCHHHHHHHHHCCCCHHHHHHHHH
VAKINDRDLARLAREEKDANYQATRDIGKLGIERFYEDILHGTAGYQEVEVNSRGRVIRT
HHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEE
LKYVPPIPGKDIVLNLDIKLQKFVEKQLDGRRGSAIVLDPKTSAVLAMVSSPSYDPNLFV
EECCCCCCCCCEEEEECHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEECCCCCCCCEEE
HGISSKNYSALLNDPDRPLVNRSTLGIYPPASTVKPFIAVAALKEKIVTTETTRDDPGYW
EEECCCCCEEECCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
RIPNSKTKPFRDWKRWGHGTVNLKQSIEESVDTFFYQIAYDMGIDRLSTWMMKFGFGDYT
ECCCCCCCCHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCC
GIDIREESKANMPTREWKVARFRTPWYQGDTIPVGIGQGYWTATPMQLAKATAVLTNHGK
CCEECCCCCCCCCCHHEEEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHEECCCC
VQAPHLLKGIIENNKEVPYEPEPYPPIEGVKDSYWQLAIDGMILVNNGRKGTARRAFAGT
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCEEEEECCCCCCCCCEECCC
PYVSAGKSGTSQVFGLKEDEEYNADEIAEHLRDHALFTGFAPAKDPKVIISLVLENGGGG
CCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCEEEEEEEEECCCCC
SSQGGPIARHVFDYVLLPKTEEAAKK
CCCCCHHHHHHHHHHCCCCCHHHHCC
>Mature Secondary Structure
MIKRKRTQIRDYHAETRLFASRAIISFSGIIVLIGLLLTNLYNIQINEFQDYKTRSNDNR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHCCCCCCE
IKIVPIAPNRGLIFDRSGNPLAENRPVFNLEVTPDKVSDLSLLFTELQTILPITQDNIDD
EEEEEECCCCCEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHH
FQKERRRTRRFKSVPLLTQLTQEQVAIFSVNQHKFQGVEIKAYLKRFYPYGKTLTHVLGY
HHHHHHHHHHHHCCCHHHHHHHHCEEEEEECCHHCCCCHHHHHHHHHCCCCHHHHHHHHH
VAKINDRDLARLAREEKDANYQATRDIGKLGIERFYEDILHGTAGYQEVEVNSRGRVIRT
HHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEE
LKYVPPIPGKDIVLNLDIKLQKFVEKQLDGRRGSAIVLDPKTSAVLAMVSSPSYDPNLFV
EECCCCCCCCCEEEEECHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEECCCCCCCCEEE
HGISSKNYSALLNDPDRPLVNRSTLGIYPPASTVKPFIAVAALKEKIVTTETTRDDPGYW
EEECCCCCEEECCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
RIPNSKTKPFRDWKRWGHGTVNLKQSIEESVDTFFYQIAYDMGIDRLSTWMMKFGFGDYT
ECCCCCCCCHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCC
GIDIREESKANMPTREWKVARFRTPWYQGDTIPVGIGQGYWTATPMQLAKATAVLTNHGK
CCEECCCCCCCCCCHHEEEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHEECCCC
VQAPHLLKGIIENNKEVPYEPEPYPPIEGVKDSYWQLAIDGMILVNNGRKGTARRAFAGT
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCEEEEECCCCCCCCCEECCC
PYVSAGKSGTSQVFGLKEDEEYNADEIAEHLRDHALFTGFAPAKDPKVIISLVLENGGGG
CCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCEEEEEEEEECCCCC
SSQGGPIARHVFDYVLLPKTEEAAKK
CCCCCHHHHHHHHHHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]