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The map label for this gene is ureF

Identifier: 59711278

GI number: 59711278

Start: 734548

End: 735213

Strand: Reverse

Name: ureF

Synonym: VF_0671

Alternate gene names: 59711278

Gene position: 735213-734548 (Counterclockwise)

Preceding gene: 59711279

Following gene: 59711277

Centisome position: 25.37

GC content: 42.64

Gene sequence:

>666_bases
GTGTTAGAGGATTTACGGCTCTACCAATTAATTAGCCCATCGTTACCTGTTGGCTCATTCACCTATTCACAAGGTTTAGA
ATGGGCGATTGAAAAAGGCTGGGTAACCAATGTAACTGAGCTAAAACATTGGTTAAGTAATCAATTAATGGACAGCCTAG
CAACCCTAGAGTTGCCTGTTTTAGCCAAATTAACTCAGTTACTGCAGCAAGAAGAGTGGCAACAAGCTCAAGATTGGTGT
GATTTTATTATCGCTAATCGAGAAACTAAAGAGTTACGCCTTGAAGAAAGACAAAGAGGACTCGCCTTCTCTATGTTATT
ACCAAAATTAGGGATCGAATTAAATCAAACCACCCTACCAATGGTAAAACAAACTCAAGTTGCTGCTTTTGCGTTAGCCG
CTAACCATTGGAACTTAACACCAACCAAACTCGCTGCAGCGTATGCGTGGGGATGGTTAGAGAATGCCGTTATTGTCGGC
ATTAAATTGGTTCCATTAGGACAAAGTGCCGGACAACAACTGCTATTAGAAATGGCTGATGTTATCCCGCAAGCAGTAGA
AAAATCACAGCACTGGCCTGAACACTTGATAGGTAGCTTCACCCCAGCGCAAGTATTGGCAAGCTCTCGACACGAGAGCC
AATACACTCGACTATTTAGATCGTGA

Upstream 100 bases:

>100_bases
ACGTAAAAGTAGAGCAAGCGAAATACCAACCAGAGCCAGGTGCTTACGGTGGTTCAAGTGCAGGCTCACACGATGGTCAT
CACCACTAAGGGCGGCCATT

Downstream 100 bases:

>100_bases
GAAAATAAAATGCAAGATTACAAACAACCGTTACGTATTGGTGTTGGTGGCCCTGTTGGTTCAGGTAAAACAGCACTATT
AGAAGTACTGTGTAAAACAC

Product: urease accessory protein UreF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MLEDLRLYQLISPSLPVGSFTYSQGLEWAIEKGWVTNVTELKHWLSNQLMDSLATLELPVLAKLTQLLQQEEWQQAQDWC
DFIIANRETKELRLEERQRGLAFSMLLPKLGIELNQTTLPMVKQTQVAAFALAANHWNLTPTKLAAAYAWGWLENAVIVG
IKLVPLGQSAGQQLLLEMADVIPQAVEKSQHWPEHLIGSFTPAQVLASSRHESQYTRLFRS

Sequences:

>Translated_221_residues
MLEDLRLYQLISPSLPVGSFTYSQGLEWAIEKGWVTNVTELKHWLSNQLMDSLATLELPVLAKLTQLLQQEEWQQAQDWC
DFIIANRETKELRLEERQRGLAFSMLLPKLGIELNQTTLPMVKQTQVAAFALAANHWNLTPTKLAAAYAWGWLENAVIVG
IKLVPLGQSAGQQLLLEMADVIPQAVEKSQHWPEHLIGSFTPAQVLASSRHESQYTRLFRS
>Mature_221_residues
MLEDLRLYQLISPSLPVGSFTYSQGLEWAIEKGWVTNVTELKHWLSNQLMDSLATLELPVLAKLTQLLQQEEWQQAQDWC
DFIIANRETKELRLEERQRGLAFSMLLPKLGIELNQTTLPMVKQTQVAAFALAANHWNLTPTKLAAAYAWGWLENAVIVG
IKLVPLGQSAGQQLLLEMADVIPQAVEKSQHWPEHLIGSFTPAQVLASSRHESQYTRLFRS

Specific function: Required for maturation of urease via the functional incorporation of the urease nickel metallocenter

COG id: COG0830

COG function: function code O; Urease accessory protein UreF

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ureF family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UREF_VIBF1 (Q5E730)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_204054.1
- STRING:   Q5E730
- GeneID:   3277360
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_0671
- NMPDR:   fig|312309.3.peg.1170
- eggNOG:   COG0830
- HOGENOM:   HBG709295
- OMA:   SPQLPIG
- ProtClustDB:   CLSK936965
- BioCyc:   VFIS312309:VF0671-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01385
- InterPro:   IPR002639
- PIRSF:   PIRSF009467

Pfam domain/function: PF01730 UreF

EC number: NA

Molecular weight: Translated: 25059; Mature: 25059

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEDLRLYQLISPSLPVGSFTYSQGLEWAIEKGWVTNVTELKHWLSNQLMDSLATLELPV
CCCHHHHHHHHCCCCCCCCCHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LAKLTQLLQQEEWQQAQDWCDFIIANRETKELRLEERQRGLAFSMLLPKLGIELNQTTLP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHCCEECCCCCC
MVKQTQVAAFALAANHWNLTPTKLAAAYAWGWLENAVIVGIKLVPLGQSAGQQLLLEMAD
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEEEEEEECCCHHHHHHHHHHHH
VIPQAVEKSQHWPEHLIGSFTPAQVLASSRHESQYTRLFRS
HHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLEDLRLYQLISPSLPVGSFTYSQGLEWAIEKGWVTNVTELKHWLSNQLMDSLATLELPV
CCCHHHHHHHHCCCCCCCCCHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LAKLTQLLQQEEWQQAQDWCDFIIANRETKELRLEERQRGLAFSMLLPKLGIELNQTTLP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHCCEECCCCCC
MVKQTQVAAFALAANHWNLTPTKLAAAYAWGWLENAVIVGIKLVPLGQSAGQQLLLEMAD
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEEEEEEECCCHHHHHHHHHHHH
VIPQAVEKSQHWPEHLIGSFTPAQVLASSRHESQYTRLFRS
HHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA