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The map label for this gene is rimK [H]

Identifier: 59711265

GI number: 59711265

Start: 722775

End: 723680

Strand: Reverse

Name: rimK [H]

Synonym: VF_0658

Alternate gene names: 59711265

Gene position: 723680-722775 (Counterclockwise)

Preceding gene: 59711266

Following gene: 172087673

Centisome position: 24.98

GC content: 41.61

Gene sequence:

>906_bases
ATGAAAATCGGTATTTTATCGCGTAACCAATCTCTTTACTCTACTAGCCGTTTAATTGAAGCTGCTGAGAGCCGTGGGCA
TGAAGTAAAAGTGATTGATGCATTACGTTGTTATATGAATATCAATTCAGAAAAACCTCAAATTCACTTCAAAGGTGAAG
AGCTGGTTGATTACGATGCAATCATTCCACGTATTGGTGCTTCTGTTACTTTCTACGGTACTGCAGTATTACGTCAATTT
GAAATGATGGGTGTATACCCAGTAAACGAATCTGTTGCAATTACTCGTTCTCGTGACAAATTACGTTCAATGCAATTACT
GTCACGTAAAGGCATCGGTATGCCTATTACTGGCTTTGCAAGTAAACCAGATGACGTTAAAGATTTATTAGACATGGTTG
GCGGTGCGCCAGTTGTGATCAAGCTCTTAGAAGGCACTCAAGGTATTGGTGTTGTTCTTGCAGAAACGCGTAAAGCAGCA
GAAAGTGTTGTTGAAGCATTCATGGGCTTAAAAGCAAATATCATGGTTCAAGAGTTCATCAAAGAAGCTGGCGGTGCTGA
TATCCGTTGCTTCGTTATTGGTGGCAAAGTGATTGCAGCAATGAAACGTCAAGGTGCAGAAGGCGAGTTCCGCTCTAACT
TACACCGTGGCGGTTCTGCATCACTGGTTAAATTAACTCCTGAAGAGCGTAAGACAGCTGTAGCTGCTGCAAATATCATG
GGCTTAAATGTTGCAGGTGTAGATTTACTTCGCTCTGATCGTGGTCCACTGGTTATGGAAGTGAACTCATCTCCGGGCTT
AGAAGGCATTGAAAAAGCAACAGGTAAAGATGTTGCAGGCCTAATTATTGATTTTATTGAGAAAAATGCAGCGACTAAAA
GGACTAAAACTCGTGGCAAAGGCTAA

Upstream 100 bases:

>100_bases
CAATGGTGTTTCGTATGTTACTTGGAAGAACCTCAATGCAAGACCGCATTGTTGTAGATCCATCGTCATCTTACCTTGTT
GATTTTGAGGAAACTGTATA

Downstream 100 bases:

>100_bases
AAAAAATCAAGCGTTTGAATTATTAACGCACTCGGTTGCACCTGGTGAAAGAAAGGTAATTGAAATTGAAGCCGCTAAGC
TTTATACGCATTCACCTTTA

Product: ribosomal protein S6 modification protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MKIGILSRNQSLYSTSRLIEAAESRGHEVKVIDALRCYMNINSEKPQIHFKGEELVDYDAIIPRIGASVTFYGTAVLRQF
EMMGVYPVNESVAITRSRDKLRSMQLLSRKGIGMPITGFASKPDDVKDLLDMVGGAPVVIKLLEGTQGIGVVLAETRKAA
ESVVEAFMGLKANIMVQEFIKEAGGADIRCFVIGGKVIAAMKRQGAEGEFRSNLHRGGSASLVKLTPEERKTAVAAANIM
GLNVAGVDLLRSDRGPLVMEVNSSPGLEGIEKATGKDVAGLIIDFIEKNAATKRTKTRGKG

Sequences:

>Translated_301_residues
MKIGILSRNQSLYSTSRLIEAAESRGHEVKVIDALRCYMNINSEKPQIHFKGEELVDYDAIIPRIGASVTFYGTAVLRQF
EMMGVYPVNESVAITRSRDKLRSMQLLSRKGIGMPITGFASKPDDVKDLLDMVGGAPVVIKLLEGTQGIGVVLAETRKAA
ESVVEAFMGLKANIMVQEFIKEAGGADIRCFVIGGKVIAAMKRQGAEGEFRSNLHRGGSASLVKLTPEERKTAVAAANIM
GLNVAGVDLLRSDRGPLVMEVNSSPGLEGIEKATGKDVAGLIIDFIEKNAATKRTKTRGKG
>Mature_301_residues
MKIGILSRNQSLYSTSRLIEAAESRGHEVKVIDALRCYMNINSEKPQIHFKGEELVDYDAIIPRIGASVTFYGTAVLRQF
EMMGVYPVNESVAITRSRDKLRSMQLLSRKGIGMPITGFASKPDDVKDLLDMVGGAPVVIKLLEGTQGIGVVLAETRKAA
ESVVEAFMGLKANIMVQEFIKEAGGADIRCFVIGGKVIAAMKRQGAEGEFRSNLHRGGSASLVKLTPEERKTAVAAANIM
GLNVAGVDLLRSDRGPLVMEVNSSPGLEGIEKATGKDVAGLIIDFIEKNAATKRTKTRGKG

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6 [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI164663810, Length=214, Percent_Identity=31.7757009345794, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI134254462, Length=213, Percent_Identity=31.4553990610329, Blast_Score=92, Evalue=5e-19,
Organism=Escherichia coli, GI1787076, Length=293, Percent_Identity=65.8703071672355, Blast_Score=417, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 32408; Mature: 32408

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGILSRNQSLYSTSRLIEAAESRGHEVKVIDALRCYMNINSEKPQIHFKGEELVDYDA
CEEEEECCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCEEEECCCHHCCHHH
IIPRIGASVTFYGTAVLRQFEMMGVYPVNESVAITRSRDKLRSMQLLSRKGIGMPITGFA
HHHHHCCCEEEHHHHHHHHHHHHEEEECCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCC
SKPDDVKDLLDMVGGAPVVIKLLEGTQGIGVVLAETRKAAESVVEAFMGLKANIMVQEFI
CCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHH
KEAGGADIRCFVIGGKVIAAMKRQGAEGEFRSNLHRGGSASLVKLTPEERKTAVAAANIM
HHCCCCCEEEEEECCHHHHHHHHCCCCCHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHE
GLNVAGVDLLRSDRGPLVMEVNSSPGLEGIEKATGKDVAGLIIDFIEKNAATKRTKTRGK
ECEEHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCHHHHHHHHHHHCCCHHHHHHCCCC
G
C
>Mature Secondary Structure
MKIGILSRNQSLYSTSRLIEAAESRGHEVKVIDALRCYMNINSEKPQIHFKGEELVDYDA
CEEEEECCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCEEEECCCHHCCHHH
IIPRIGASVTFYGTAVLRQFEMMGVYPVNESVAITRSRDKLRSMQLLSRKGIGMPITGFA
HHHHHCCCEEEHHHHHHHHHHHHEEEECCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCC
SKPDDVKDLLDMVGGAPVVIKLLEGTQGIGVVLAETRKAAESVVEAFMGLKANIMVQEFI
CCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHH
KEAGGADIRCFVIGGKVIAAMKRQGAEGEFRSNLHRGGSASLVKLTPEERKTAVAAANIM
HHCCCCCEEEEEECCHHHHHHHHCCCCCHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHE
GLNVAGVDLLRSDRGPLVMEVNSSPGLEGIEKATGKDVAGLIIDFIEKNAATKRTKTRGK
ECEEHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCHHHHHHHHHHHCCCHHHHHHCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA