Click here to switch to the map view.

The map label for this gene is queF

Identifier: 59711205

GI number: 59711205

Start: 658364

End: 659209

Strand: Reverse

Name: queF

Synonym: VF_0598

Alternate gene names: 59711205

Gene position: 659209-658364 (Counterclockwise)

Preceding gene: 59711208

Following gene: 59711204

Centisome position: 22.75

GC content: 38.42

Gene sequence:

>846_bases
ATGACTAAATACACCAATGCAGATGAGCTAAAATCACTGACCCTTGGACAAAAAACAGAATACAAACACACTTACGAGCC
TGAATTACTACAAGCGGTCCCTCGAAGTCTTAATCGTGATGATCTTGCACTAGGTGATGAACTTCCTTTTGTAGGTTGTG
ATGTATGGACGCTATATGAACTGTCGTGGCTAAATCAAAATGGTTTACCACAAGTGGCTGTTGGTGAAGTAGCACTACCT
GCAACGAGCCCAAACCTTGTTGAATCAAAATCGTTTAAACTTTATCTAAACAGTTTTAACCAAACAAAATTCACCTCATG
GGATGAAGTAAAAGAGACATTGGTAAAAGATTTAAGTGCATGTGCAGGTGAAACCGTAAAAGTAGATATATTCCCTGTAC
AACGATACTCACAACAGCCAATTGTCGATATGCAGGGCGAATGCATTGATGATCAAGATATTATTATTGATGACTACGAG
TTCAATGCTGCTTATTTAGAGTCAAGTACATCTGACGTAAATATCGAAGAAACACTGCACAGTCATTTATTGAAATCTAA
CTGCCTAATTACAAACCAACCCGATTGGGGCAGTGTTGAAATTCAATACAAAGGCAAAAAAATTGATCGTGAGAAGCTAC
TACGTTATTTAATCTCTTTCCGTCAACATAATGAGTTTCATGAACAATGTGTAGAACGAATTTACACGGATATTATGAAA
TATTGCGCACCAGAATCATTAACTGTATTTGCTCGTTATACGCGACGCGGTGGTCTCGATATCAATCCATTCCGTAGCTC
GCACCTATTGGCACCTAAAGACAATTTACGTTTAGCTCGCCAGTAA

Upstream 100 bases:

>100_bases
TTCTCCTCATAAGATAAAATTTCAAGTAAACTATCCAATATTAAACGAAAGGCTGTCTACTTAGTACGCCTTATTCTAAT
CTACTTTTGGTATTTTCAAT

Downstream 100 bases:

>100_bases
GACATTTTGATTAATTTTGTCTACAAAGAACGTTTCTTTGTAGACAAATCCCCTCACAACGATAGTATGTAATGGTTGTT
TTTTTGTGGATCCTTTACAT

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MTKYTNADELKSLTLGQKTEYKHTYEPELLQAVPRSLNRDDLALGDELPFVGCDVWTLYELSWLNQNGLPQVAVGEVALP
ATSPNLVESKSFKLYLNSFNQTKFTSWDEVKETLVKDLSACAGETVKVDIFPVQRYSQQPIVDMQGECIDDQDIIIDDYE
FNAAYLESSTSDVNIEETLHSHLLKSNCLITNQPDWGSVEIQYKGKKIDREKLLRYLISFRQHNEFHEQCVERIYTDIMK
YCAPESLTVFARYTRRGGLDINPFRSSHLLAPKDNLRLARQ

Sequences:

>Translated_281_residues
MTKYTNADELKSLTLGQKTEYKHTYEPELLQAVPRSLNRDDLALGDELPFVGCDVWTLYELSWLNQNGLPQVAVGEVALP
ATSPNLVESKSFKLYLNSFNQTKFTSWDEVKETLVKDLSACAGETVKVDIFPVQRYSQQPIVDMQGECIDDQDIIIDDYE
FNAAYLESSTSDVNIEETLHSHLLKSNCLITNQPDWGSVEIQYKGKKIDREKLLRYLISFRQHNEFHEQCVERIYTDIMK
YCAPESLTVFARYTRRGGLDINPFRSSHLLAPKDNLRLARQ
>Mature_280_residues
TKYTNADELKSLTLGQKTEYKHTYEPELLQAVPRSLNRDDLALGDELPFVGCDVWTLYELSWLNQNGLPQVAVGEVALPA
TSPNLVESKSFKLYLNSFNQTKFTSWDEVKETLVKDLSACAGETVKVDIFPVQRYSQQPIVDMQGECIDDQDIIIDDYEF
NAAYLESSTSDVNIEETLHSHLLKSNCLITNQPDWGSVEIQYKGKKIDREKLLRYLISFRQHNEFHEQCVERIYTDIMKY
CAPESLTVFARYTRRGGLDINPFRSSHLLAPKDNLRLARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=283, Percent_Identity=63.2508833922262, Blast_Score=374, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_VIBF1 (Q5E7A3)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_203981.1
- ProteinModelPortal:   Q5E7A3
- SMR:   Q5E7A3
- STRING:   Q5E7A3
- GeneID:   3278306
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_0598
- NMPDR:   fig|312309.3.peg.694
- eggNOG:   COG0780
- HOGENOM:   HBG289139
- OMA:   PFRSNFE
- PhylomeDB:   Q5E7A3
- ProtClustDB:   PRK11792
- BioCyc:   VFIS312309:VF0598-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00817
- InterPro:   IPR016428
- InterPro:   IPR020602
- PIRSF:   PIRSF004750
- TIGRFAMs:   TIGR03138

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 32315; Mature: 32184

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKYTNADELKSLTLGQKTEYKHTYEPELLQAVPRSLNRDDLALGDELPFVGCDVWTLYE
CCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEECCCHHHHEE
LSWLNQNGLPQVAVGEVALPATSPNLVESKSFKLYLNSFNQTKFTSWDEVKETLVKDLSA
EEECCCCCCCCEEECCEECCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHH
CAGETVKVDIFPVQRYSQQPIVDMQGECIDDQDIIIDDYEFNAAYLESSTSDVNIEETLH
HCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEECCCCCCCCHHHHHH
SHLLKSNCLITNQPDWGSVEIQYKGKKIDREKLLRYLISFRQHNEFHEQCVERIYTDIMK
HHHHHCCCEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
YCAPESLTVFARYTRRGGLDINPFRSSHLLAPKDNLRLARQ
HCCCCCCEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TKYTNADELKSLTLGQKTEYKHTYEPELLQAVPRSLNRDDLALGDELPFVGCDVWTLYE
CCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEECCCHHHHEE
LSWLNQNGLPQVAVGEVALPATSPNLVESKSFKLYLNSFNQTKFTSWDEVKETLVKDLSA
EEECCCCCCCCEEECCEECCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHH
CAGETVKVDIFPVQRYSQQPIVDMQGECIDDQDIIIDDYEFNAAYLESSTSDVNIEETLH
HCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEECCCCCCCCHHHHHH
SHLLKSNCLITNQPDWGSVEIQYKGKKIDREKLLRYLISFRQHNEFHEQCVERIYTDIMK
HHHHHCCCEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
YCAPESLTVFARYTRRGGLDINPFRSSHLLAPKDNLRLARQ
HCCCCCCEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA