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The map label for this gene is dacB [H]

Identifier: 59711081

GI number: 59711081

Start: 509280

End: 510686

Strand: Reverse

Name: dacB [H]

Synonym: VF_0474

Alternate gene names: 59711081

Gene position: 510686-509280 (Counterclockwise)

Preceding gene: 59711082

Following gene: 59711071

Centisome position: 17.62

GC content: 37.03

Gene sequence:

>1407_bases
ATGTTTTTTTCTCGTTACTCCCTTATCGCACTCAGTCTTATTTCTTTTTCAAGTATTGCTGATATCAAAGAGACACTTTC
ATCTCTCCCATCTGGTAGCAGAACCGCTATTACTGTCGAAAATCTACAATCAAATAAGATAGAGTTTCAACAAAAACAAG
ATGAACTATTGCCACCAGCAAGCACCCTAAAAATTGCCACTGCATTAGCGGCTCGTTTAGCTTTAGGGACCGAATTCACT
TTTACAACAACATTATATAAAGCGGATAAAGATGTTGTTTTGACTTTCTCTGGCGATCCTTCTCTATCACGAAAACAACT
TTCTGAGCTATTTGATGATAGTGAACTAAAAACCATACAAGGTGATTTATGGATCGATGGAAGTATCTTTACTGGATATG
AGCGAGCAGTGGGCTGGCCTTGGGATATTTTAGGCGTCTGTTATAGCGCACCATCAAGTGCATTAAGTATTGATGAAAAC
TGCGTTCAAGCTTCAATCACCACTCATGAGGATGGAAGTACCCGAGTCTTTGTGCCTATTCATCAGCCTATTTCTTTTTC
TAGCTATGCTAAAAGTGTGAGCAGAGAAGAACAAAAATCGACGTTCTGCGACTTAGAGTTAACCACTCAAGGTAACCATT
ATGAGCTAAACGGTTGTTTAGTTAGGCGTAATAAGCCTCTGCCGTTAAAATTTGCTGTGCAAGATACAAGAGATTTCACA
AAAAGAACCGTATCTAAAATCCTAGCTGAACATAATATTAAACTTAATGGTGAAATTCATTTTGGTGCACCAAAGTCTGA
ATTAACCAACTTAAATGTATTAGCTAACCATAACTCTAAACCATTAATGGAATTATTGGACCACATGCTCAAAAAGTCTG
ATAACCATTATGCTGATAACTTGACTAAGATGTTAGGCCATTTAAATTACCAGCAGCCAGGTAGCTTCACCAATGGCACA
GCTGCAATTAAAGACATCTTATTAAAGCAAGCGGATATTGATTTAAGTAATGCCGTATTAGTTGATGGTTCTGGTTTATC
ACGTAATAACCGCATGACAGCTAATCAAATGAAAAGCATTCTTCAATTCATTCATAAATACGATAAAGAGCTAGGTTTAA
TTGAACTATTACCAACCTCTGGTATTGATGGCACCCTAAAGTATCGTAATAGCATGCGTAAAGAGCCAATTAAAGGCAGC
ATGAAATCCAAGAGTGGTTCACTATTTGGTAGCTATAATATGGCGGGATTTGTTGGTTTAGAAAATAAAGAGCCTCAGCT
GTTCGTTCAATTTGTTGCAGATTATCACCCTGTTGTAGCAAAAGAAGGAACAATTCCGGTTGAACGACCAATAGATTCCT
TTGAGAAAAGCTTTTATCAAGAATTAATTAAATTATCACAAGAGTAA

Upstream 100 bases:

>100_bases
GCGTTTTTTATTTGTGACGCCTTCTGAAATTTTTCTAATTCATTACACATCATTATCAACAGACGTTAAGATACCTCATT
ATTTTCAAATTGAGCTCATC

Downstream 100 bases:

>100_bases
CGTTTTCTAGCAATTAGTAATACCAATTCCATTAATAATCTAATCTGTTTAGTTATCTTGGTATAAACCAAAAAAATCCC
GAAGAACTCTCATTCTTCGG

Product: D-alanyl-D-alanine carboxypeptidase/endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MFFSRYSLIALSLISFSSIADIKETLSSLPSGSRTAITVENLQSNKIEFQQKQDELLPPASTLKIATALAARLALGTEFT
FTTTLYKADKDVVLTFSGDPSLSRKQLSELFDDSELKTIQGDLWIDGSIFTGYERAVGWPWDILGVCYSAPSSALSIDEN
CVQASITTHEDGSTRVFVPIHQPISFSSYAKSVSREEQKSTFCDLELTTQGNHYELNGCLVRRNKPLPLKFAVQDTRDFT
KRTVSKILAEHNIKLNGEIHFGAPKSELTNLNVLANHNSKPLMELLDHMLKKSDNHYADNLTKMLGHLNYQQPGSFTNGT
AAIKDILLKQADIDLSNAVLVDGSGLSRNNRMTANQMKSILQFIHKYDKELGLIELLPTSGIDGTLKYRNSMRKEPIKGS
MKSKSGSLFGSYNMAGFVGLENKEPQLFVQFVADYHPVVAKEGTIPVERPIDSFEKSFYQELIKLSQE

Sequences:

>Translated_468_residues
MFFSRYSLIALSLISFSSIADIKETLSSLPSGSRTAITVENLQSNKIEFQQKQDELLPPASTLKIATALAARLALGTEFT
FTTTLYKADKDVVLTFSGDPSLSRKQLSELFDDSELKTIQGDLWIDGSIFTGYERAVGWPWDILGVCYSAPSSALSIDEN
CVQASITTHEDGSTRVFVPIHQPISFSSYAKSVSREEQKSTFCDLELTTQGNHYELNGCLVRRNKPLPLKFAVQDTRDFT
KRTVSKILAEHNIKLNGEIHFGAPKSELTNLNVLANHNSKPLMELLDHMLKKSDNHYADNLTKMLGHLNYQQPGSFTNGT
AAIKDILLKQADIDLSNAVLVDGSGLSRNNRMTANQMKSILQFIHKYDKELGLIELLPTSGIDGTLKYRNSMRKEPIKGS
MKSKSGSLFGSYNMAGFVGLENKEPQLFVQFVADYHPVVAKEGTIPVERPIDSFEKSFYQELIKLSQE
>Mature_468_residues
MFFSRYSLIALSLISFSSIADIKETLSSLPSGSRTAITVENLQSNKIEFQQKQDELLPPASTLKIATALAARLALGTEFT
FTTTLYKADKDVVLTFSGDPSLSRKQLSELFDDSELKTIQGDLWIDGSIFTGYERAVGWPWDILGVCYSAPSSALSIDEN
CVQASITTHEDGSTRVFVPIHQPISFSSYAKSVSREEQKSTFCDLELTTQGNHYELNGCLVRRNKPLPLKFAVQDTRDFT
KRTVSKILAEHNIKLNGEIHFGAPKSELTNLNVLANHNSKPLMELLDHMLKKSDNHYADNLTKMLGHLNYQQPGSFTNGT
AAIKDILLKQADIDLSNAVLVDGSGLSRNNRMTANQMKSILQFIHKYDKELGLIELLPTSGIDGTLKYRNSMRKEPIKGS
MKSKSGSLFGSYNMAGFVGLENKEPQLFVQFVADYHPVVAKEGTIPVERPIDSFEKSFYQELIKLSQE

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=481, Percent_Identity=33.6798336798337, Blast_Score=298, Evalue=4e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 52100; Mature: 52100

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFSRYSLIALSLISFSSIADIKETLSSLPSGSRTAITVENLQSNKIEFQQKQDELLPPA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCH
STLKIATALAARLALGTEFTFTTTLYKADKDVVLTFSGDPSLSRKQLSELFDDSELKTIQ
HHHHHHHHHHHHHHHCCCEEEEEEEEECCCCEEEEECCCCCCCHHHHHHHHCCCCCEEEE
GDLWIDGSIFTGYERAVGWPWDILGVCYSAPSSALSIDENCVQASITTHEDGSTRVFVPI
CCEEECCCEECCHHHHCCCCHHHHHHHHCCCCCCEECCCHHHEEEEEECCCCCEEEEEEE
HQPISFSSYAKSVSREEQKSTFCDLELTTQGNHYELNGCLVRRNKPLPLKFAVQDTRDFT
CCCCCHHHHHHHHHHHHHHCCEEEEEEEECCCEEEECEEEEECCCCCCEEEEECCHHHHH
KRTVSKILAEHNIKLNGEIHFGAPKSELTNLNVLANHNSKPLMELLDHMLKKSDNHYADN
HHHHHHHHHHCCEEECCEEEECCCHHHHCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHH
LTKMLGHLNYQQPGSFTNGTAAIKDILLKQADIDLSNAVLVDGSGLSRNNRMTANQMKSI
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHH
LQFIHKYDKELGLIELLPTSGIDGTLKYRNSMRKEPIKGSMKSKSGSLFGSYNMAGFVGL
HHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEECCCCEEEEEC
ENKEPQLFVQFVADYHPVVAKEGTIPVERPIDSFEKSFYQELIKLSQE
CCCCHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFFSRYSLIALSLISFSSIADIKETLSSLPSGSRTAITVENLQSNKIEFQQKQDELLPPA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCH
STLKIATALAARLALGTEFTFTTTLYKADKDVVLTFSGDPSLSRKQLSELFDDSELKTIQ
HHHHHHHHHHHHHHHCCCEEEEEEEEECCCCEEEEECCCCCCCHHHHHHHHCCCCCEEEE
GDLWIDGSIFTGYERAVGWPWDILGVCYSAPSSALSIDENCVQASITTHEDGSTRVFVPI
CCEEECCCEECCHHHHCCCCHHHHHHHHCCCCCCEECCCHHHEEEEEECCCCCEEEEEEE
HQPISFSSYAKSVSREEQKSTFCDLELTTQGNHYELNGCLVRRNKPLPLKFAVQDTRDFT
CCCCCHHHHHHHHHHHHHHCCEEEEEEEECCCEEEECEEEEECCCCCCEEEEECCHHHHH
KRTVSKILAEHNIKLNGEIHFGAPKSELTNLNVLANHNSKPLMELLDHMLKKSDNHYADN
HHHHHHHHHHCCEEECCEEEECCCHHHHCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHH
LTKMLGHLNYQQPGSFTNGTAAIKDILLKQADIDLSNAVLVDGSGLSRNNRMTANQMKSI
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHH
LQFIHKYDKELGLIELLPTSGIDGTLKYRNSMRKEPIKGSMKSKSGSLFGSYNMAGFVGL
HHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEECCCCEEEEEC
ENKEPQLFVQFVADYHPVVAKEGTIPVERPIDSFEKSFYQELIKLSQE
CCCCHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2040429; 9278503; 2046551 [H]