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The map label for this gene is thiF [H]

Identifier: 58617616

GI number: 58617616

Start: 1420492

End: 1421274

Strand: Reverse

Name: thiF [H]

Synonym: ERGA_CDS_08890

Alternate gene names: 58617616

Gene position: 1421274-1420492 (Counterclockwise)

Preceding gene: 58617617

Following gene: 58617615

Centisome position: 94.76

GC content: 30.78

Gene sequence:

>783_bases
GTGTTTGAGCGGTATAAAAAGCAGATTTTACTTTCAGAAGTAGGAGTAGTAGGTCAGGATAAGTTATGTAATAGTAGTGT
TCTAATAGTTGGATGTGGTGGTTTGGGAAGTACAGTTATTCCTTTACTTGCAGCTAGTGGTATTGGTAGCTTGATATTAT
CTGATAGTGATAAAATTCAAATATCAAATTTGAATAGGCAGATAATATATAAAGAATCTGATGTTAGTAATAGTAAAGTG
GTAACAGCTGCTAAATTTGTTAGTATGCTTAATTCTGCAGTAAATGTACGTACATTGAATGTCTTTATTACTCCTAAAAA
CTTTGAACAAGTTTTTAGAGAAGTAGATCTTGTTGTAGATTGTACAGATCGTTTAGCAACTAAGTTATTTCTTAATGATG
CATGTGTTATTTTAGGAAAACCTTTAATACATAGTGCAGCAATTGGTTTTGTTGGTCAAATATTAACCATATATCCATAT
GGTAAACCTTGCCTCAGATGTTTTTTTGAACATCAGTATATGAGTATACATCTAAACTGTTCTAATGCTGGTGTGCTAGG
GCCAACAGTTGGTGTAGTAGGTAGTATTGTAGCAGCAGAAGCTATAAAGTATTTATTAGAAATTTCATATAATGTAATTG
GAAAGTTACAGAGGATTGACTTAAAAAATAATGAATTTACTCATTACACATTTCAAAAAAACGAGAGATGTATAGTGTGT
AGCAATGATGTTAAAGTGGATCCATATGATTATGATTATTATGAAAGTAAGTTGTATTTTTAG

Upstream 100 bases:

>100_bases
GTTAAATGTAAAAAGTTATGTTGATGAAGATATTCCAGAATATTTAAGAAGAATTCCTGTATCTACACCTGGAAGTAGAA
AATATTTGAGTGAATAGTTA

Downstream 100 bases:

>100_bases
TAAAAAATTCTTGATCAATTGTTATAATATATTGGTGATGAATTAAATGTATAACACGTAGTAGTCTTTTTGAAATTGAT
CTGTATGTGTAGTTATTAGT

Product: adenylyltransferase

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MFERYKKQILLSEVGVVGQDKLCNSSVLIVGCGGLGSTVIPLLAASGIGSLILSDSDKIQISNLNRQIIYKESDVSNSKV
VTAAKFVSMLNSAVNVRTLNVFITPKNFEQVFREVDLVVDCTDRLATKLFLNDACVILGKPLIHSAAIGFVGQILTIYPY
GKPCLRCFFEHQYMSIHLNCSNAGVLGPTVGVVGSIVAAEAIKYLLEISYNVIGKLQRIDLKNNEFTHYTFQKNERCIVC
SNDVKVDPYDYDYYESKLYF

Sequences:

>Translated_260_residues
MFERYKKQILLSEVGVVGQDKLCNSSVLIVGCGGLGSTVIPLLAASGIGSLILSDSDKIQISNLNRQIIYKESDVSNSKV
VTAAKFVSMLNSAVNVRTLNVFITPKNFEQVFREVDLVVDCTDRLATKLFLNDACVILGKPLIHSAAIGFVGQILTIYPY
GKPCLRCFFEHQYMSIHLNCSNAGVLGPTVGVVGSIVAAEAIKYLLEISYNVIGKLQRIDLKNNEFTHYTFQKNERCIVC
SNDVKVDPYDYDYYESKLYF
>Mature_260_residues
MFERYKKQILLSEVGVVGQDKLCNSSVLIVGCGGLGSTVIPLLAASGIGSLILSDSDKIQISNLNRQIIYKESDVSNSKV
VTAAKFVSMLNSAVNVRTLNVFITPKNFEQVFREVDLVVDCTDRLATKLFLNDACVILGKPLIHSAAIGFVGQILTIYPY
GKPCLRCFFEHQYMSIHLNCSNAGVLGPTVGVVGSIVAAEAIKYLLEISYNVIGKLQRIDLKNNEFTHYTFQKNERCIVC
SNDVKVDPYDYDYYESKLYF

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=255, Percent_Identity=37.6470588235294, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI23510338, Length=166, Percent_Identity=28.3132530120482, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI23510340, Length=166, Percent_Identity=28.3132530120482, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI4885649, Length=142, Percent_Identity=31.6901408450704, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI38045942, Length=157, Percent_Identity=28.6624203821656, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI38045944, Length=157, Percent_Identity=28.6624203821656, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI150417996, Length=150, Percent_Identity=24.6666666666667, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI87082356, Length=248, Percent_Identity=37.0967741935484, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI1787048, Length=241, Percent_Identity=36.5145228215768, Blast_Score=152, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17540406, Length=253, Percent_Identity=38.7351778656126, Blast_Score=174, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI193203301, Length=211, Percent_Identity=26.0663507109005, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71984476, Length=164, Percent_Identity=31.0975609756098, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI86565433, Length=166, Percent_Identity=30.7228915662651, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI86565431, Length=166, Percent_Identity=31.3253012048193, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321903, Length=214, Percent_Identity=35.981308411215, Blast_Score=142, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6320598, Length=163, Percent_Identity=31.9018404907975, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6322639, Length=168, Percent_Identity=31.547619047619, Blast_Score=79, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24582879, Length=241, Percent_Identity=39.0041493775934, Blast_Score=157, Evalue=6e-39,
Organism=Drosophila melanogaster, GI28573937, Length=166, Percent_Identity=30.7228915662651, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI20129973, Length=163, Percent_Identity=29.4478527607362, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24660640, Length=158, Percent_Identity=29.746835443038, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012731
- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 28834; Mature: 28834

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFERYKKQILLSEVGVVGQDKLCNSSVLIVGCGGLGSTVIPLLAASGIGSLILSDSDKIQ
CCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEECCCCEEE
ISNLNRQIIYKESDVSNSKVVTAAKFVSMLNSAVNVRTLNVFITPKNFEQVFREVDLVVD
EECCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHEEE
CTDRLATKLFLNDACVILGKPLIHSAAIGFVGQILTIYPYGKPCLRCFFEHQYMSIHLNC
CHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHEEEEECCCHHHHHHHHCCCEEEEEEEC
SNAGVLGPTVGVVGSIVAAEAIKYLLEISYNVIGKLQRIDLKNNEFTHYTFQKNERCIVC
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEEECCCCEEEEE
SNDVKVDPYDYDYYESKLYF
CCCCEECCCCCCHHCCEECC
>Mature Secondary Structure
MFERYKKQILLSEVGVVGQDKLCNSSVLIVGCGGLGSTVIPLLAASGIGSLILSDSDKIQ
CCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEECCCCEEE
ISNLNRQIIYKESDVSNSKVVTAAKFVSMLNSAVNVRTLNVFITPKNFEQVFREVDLVVD
EECCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHEEE
CTDRLATKLFLNDACVILGKPLIHSAAIGFVGQILTIYPYGKPCLRCFFEHQYMSIHLNC
CHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHEEEEECCCHHHHHHHHCCCEEEEEEEC
SNAGVLGPTVGVVGSIVAAEAIKYLLEISYNVIGKLQRIDLKNNEFTHYTFQKNERCIVC
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEEECCCCEEEEE
SNDVKVDPYDYDYYESKLYF
CCCCEECCCCCCHHCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 9632726; 10082377 [H]