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The map label for this gene is metB [H]

Identifier: 57652619

GI number: 57652619

Start: 437181

End: 438284

Strand: Reverse

Name: metB [H]

Synonym: SACOL0431

Alternate gene names: 57652619

Gene position: 438284-437181 (Counterclockwise)

Preceding gene: 57652624

Following gene: 57652618

Centisome position: 15.6

GC content: 35.87

Gene sequence:

>1104_bases
ATGAAGGATACACAGTTAGCCCAAATCACATTAACCGATGATTCAACCGGTGCTATAGCGAATCCAATCCATTTATCTAC
TGCCTACAAGCATCCAAAACTAGGACAATCGACAGGTTTTGATTATACACGTACTAAAAATCCTACACGCTCAACATTTG
AAACCTGTTTTGCCAAACTTGAGCATGGTATTGCATCATTCGCTACATCAAGTGGAATGTCAGCCATTCAATTAATATGT
AATCTATTTAAACCTCATGATGAAATTTTAGTTTCATTCGATTTATATGGTGGCACATTTAGATTATTTGAATTTTACGA
GCAACAATACGATATCAAATTTAAGTACGTTGATTTTACAGATTATGAACAAGTTGAAAAAGAAATCACTGATAAAACAG
TTGCATTATTCATTGAACCAATATCTAACCCACAAATGATTGCTATTGATGTAAAGCCATACTATCAACTTTGTAAAGCT
AAAGGCTTATTGTCAATTATCGACAATACTTTTTTAACACCTTATCTTTCAACACCACTAGCAGAAGGTGCTGATATAGT
CTTACATTCAGCCACGAAATATATTGGCGGACATAACGATGTACTAGCAGGTGTCGTAACCGTCAAAGATGAATCACTCG
CGCAACAGTTGTTTGATTTTCACAACATGACTGGCGCAACACTTTCACCAATAGATAGTTATTTGTTGTTACGTGGACTT
AAAACTTTGCATTTACGCATTGAGCGTGCGCAATCAAACGCTAGAAAACTTGCTAAAAAATGTCAGTCACTTCAAGCAAT
TGACGAAGTACTATATAGCGGGCAAACTGGCATGCTTAGTTTAAGACTTAACAAGGCCTATAGCGTCGCTAAATTATTAG
AAAATTTAGACATTTGCATTTTTGCAGAAAGTTTAGGAGGTACTGAAACATTAGTGACCTTCCCTTACACCCAAACACAT
GTTGATATGCCAGATGCTGAAAAAGATAAACGTGGCATTGATGAGTATTTAATCCGCTTATCACTAGGTGTTGAAAATTA
TGAAGACATCGAACGCGATATCATCCAAGCATTAGATAAAGCTCAGATTGGAGAGATTGTATGA

Upstream 100 bases:

>100_bases
GGTGGTACCGCGAAACATAAGCTTTCGTCCTTTTTATCCGATTCATTCGGGTACGAAGGACGGAAGCTTTTTTTATTTTT
TCTTATAGGAGGGTCTTAAT

Downstream 100 bases:

>100_bases
CACTTTCAAAAGAGACAGAAGTGATATTCGATTGGCGTAGAGGTGTGGAATATCATTCAGCTAACCCACCACTCTATGAT
TCTTCAACATTCCATCAAAC

Product: trans-sulfuration enzyme family protein

Products: NA

Alternate protein names: CGS; O-acetylhomoserine (thiol)-lyase; O-acetylhomoserine sulfhydrylase; OAH sulfhydrylase [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTRTKNPTRSTFETCFAKLEHGIASFATSSGMSAIQLIC
NLFKPHDEILVSFDLYGGTFRLFEFYEQQYDIKFKYVDFTDYEQVEKEITDKTVALFIEPISNPQMIAIDVKPYYQLCKA
KGLLSIIDNTFLTPYLSTPLAEGADIVLHSATKYIGGHNDVLAGVVTVKDESLAQQLFDFHNMTGATLSPIDSYLLLRGL
KTLHLRIERAQSNARKLAKKCQSLQAIDEVLYSGQTGMLSLRLNKAYSVAKLLENLDICIFAESLGGTETLVTFPYTQTH
VDMPDAEKDKRGIDEYLIRLSLGVENYEDIERDIIQALDKAQIGEIV

Sequences:

>Translated_367_residues
MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTRTKNPTRSTFETCFAKLEHGIASFATSSGMSAIQLIC
NLFKPHDEILVSFDLYGGTFRLFEFYEQQYDIKFKYVDFTDYEQVEKEITDKTVALFIEPISNPQMIAIDVKPYYQLCKA
KGLLSIIDNTFLTPYLSTPLAEGADIVLHSATKYIGGHNDVLAGVVTVKDESLAQQLFDFHNMTGATLSPIDSYLLLRGL
KTLHLRIERAQSNARKLAKKCQSLQAIDEVLYSGQTGMLSLRLNKAYSVAKLLENLDICIFAESLGGTETLVTFPYTQTH
VDMPDAEKDKRGIDEYLIRLSLGVENYEDIERDIIQALDKAQIGEIV
>Mature_367_residues
MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTRTKNPTRSTFETCFAKLEHGIASFATSSGMSAIQLIC
NLFKPHDEILVSFDLYGGTFRLFEFYEQQYDIKFKYVDFTDYEQVEKEITDKTVALFIEPISNPQMIAIDVKPYYQLCKA
KGLLSIIDNTFLTPYLSTPLAEGADIVLHSATKYIGGHNDVLAGVVTVKDESLAQQLFDFHNMTGATLSPIDSYLLLRGL
KTLHLRIERAQSNARKLAKKCQSLQAIDEVLYSGQTGMLSLRLNKAYSVAKLLENLDICIFAESLGGTETLVTFPYTQTH
VDMPDAEKDKRGIDEYLIRLSLGVENYEDIERDIIQALDKAQIGEIV

Specific function: Catalyzes the transformation of O-succinylhomoserine into cystathionine, as well as O-acetylhomoserine and sulfide into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=363, Percent_Identity=38.0165289256198, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI299473759, Length=363, Percent_Identity=34.7107438016529, Blast_Score=195, Evalue=7e-50,
Organism=Homo sapiens, GI25453487, Length=362, Percent_Identity=34.5303867403315, Blast_Score=186, Evalue=2e-47,
Organism=Escherichia coli, GI1790375, Length=371, Percent_Identity=36.9272237196765, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1789383, Length=386, Percent_Identity=23.0569948186528, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17560056, Length=363, Percent_Identity=35.5371900826446, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17538055, Length=363, Percent_Identity=36.0881542699724, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI71980708, Length=366, Percent_Identity=33.879781420765, Blast_Score=187, Evalue=8e-48,
Organism=Saccharomyces cerevisiae, GI6319307, Length=375, Percent_Identity=33.6, Blast_Score=203, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6321254, Length=406, Percent_Identity=30.0492610837438, Blast_Score=169, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6323333, Length=424, Percent_Identity=30.188679245283, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6321904, Length=386, Percent_Identity=27.720207253886, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6321170, Length=331, Percent_Identity=27.4924471299094, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI19922554, Length=365, Percent_Identity=34.5205479452055, Blast_Score=217, Evalue=1e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48; =2.5.1.49 [H]

Molecular weight: Translated: 41072; Mature: 41072

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTRTKNPTRSTFETCFAKL
CCCCEEEEEEEECCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
EHGIASFATSSGMSAIQLICNLFKPHDEILVSFDLYGGTFRLFEFYEQQYDIKFKYVDFT
HHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCC
DYEQVEKEITDKTVALFIEPISNPQMIAIDVKPYYQLCKAKGLLSIIDNTFLTPYLSTPL
CHHHHHHHHCCCEEEEEEEECCCCCEEEEECCHHHHHHHHCCHHHHHHHHHCCHHHCCCC
AEGADIVLHSATKYIGGHNDVLAGVVTVKDESLAQQLFDFHNMTGATLSPIDSYLLLRGL
CCCCCEEEEHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
KTLHLRIERAQSNARKLAKKCQSLQAIDEVLYSGQTGMLSLRLNKAYSVAKLLENLDICI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHCCCEEE
FAESLGGTETLVTFPYTQTHVDMPDAEKDKRGIDEYLIRLSLGVENYEDIERDIIQALDK
EEECCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AQIGEIV
HHCCCCC
>Mature Secondary Structure
MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTRTKNPTRSTFETCFAKL
CCCCEEEEEEEECCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
EHGIASFATSSGMSAIQLICNLFKPHDEILVSFDLYGGTFRLFEFYEQQYDIKFKYVDFT
HHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCC
DYEQVEKEITDKTVALFIEPISNPQMIAIDVKPYYQLCKAKGLLSIIDNTFLTPYLSTPL
CHHHHHHHHCCCEEEEEEEECCCCCEEEEECCHHHHHHHHCCHHHHHHHHHCCHHHCCCC
AEGADIVLHSATKYIGGHNDVLAGVVTVKDESLAQQLFDFHNMTGATLSPIDSYLLLRGL
CCCCCEEEEHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
KTLHLRIERAQSNARKLAKKCQSLQAIDEVLYSGQTGMLSLRLNKAYSVAKLLENLDICI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHCCCEEE
FAESLGGTETLVTFPYTQTHVDMPDAEKDKRGIDEYLIRLSLGVENYEDIERDIIQALDK
EEECCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AQIGEIV
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]