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The map label for this gene is dfrA [H]

Identifier: 57652588

GI number: 57652588

Start: 405468

End: 406493

Strand: Direct

Name: dfrA [H]

Synonym: SACOL0399

Alternate gene names: 57652588

Gene position: 405468-406493 (Clockwise)

Preceding gene: 57652587

Following gene: 57652593

Centisome position: 14.43

GC content: 35.58

Gene sequence:

>1026_bases
ATGAATAATAAAGTATTAGCAACCGGTGGTACAGGGTTTGTTGGCATGCGAATTATTTCACGATTATTAGAACAAGGTTA
TGACGTACAAACGACGATACGTGATTTAAGTAAAGCTGATAAAGTAATTAAAACAATGCAAGACAATGGCATTTCCACAG
AGCGATTAATGTTTGTCGAAGCGGATTTATCACAAGATGAACATTGGGATGAAGCAATGAAAGATTGCAAGTATGTCTTG
AGTGTAGCATCTCCGGTGTTTTTCGGTAAAACAGACGATGCAGAAGTGATGGCGAAGCCTGCAATTGAAGGTATACAACG
TATTTTAAGAGCTGCAGAACATGCGGGTGTTAAACGTGTGGTAATGACTGCAAACTTTGGTGCAGTTGGTTTTAGTAATA
AAGATAAAAATTCAATCACAAATGAAAGTCATTGGACAAATGAAGATGAACCAGGCTTATCAGTATATGAAAAATCAAAA
TTGTTAGCTGAAAAGGCAGCGTGGGATTTTGTTGAGAATGAAAATACAACAGTAGAATTTGCCACAATCAATCCAGTTGC
AATTTTTGGGCCATCATTAGATGCACACGTTTCAGGAAGCTTTCATTTATTAGAAAATTTATTGAATGGTTCAATGAAAC
GTGTACCGCAAATTCCGTTAAATGTTGTTGATGTGAGAGACGTAGCTGAACTGCACATTTTGGCAATGACAAATGAACAA
GCTAATGGCAAGCGATTTATTGCGACGGCTGATGGACAAATTAATTTGTTGGAAATTGCAAAATTAATTAAAGAAAAGAG
ACCTGAAATAGCTCAAAAAGTTTCTACTAAAAAATTACCAGACTTTATTTTGAGTCTAGGTGCTAAATTTAATCATCAAG
CTAAAGAAGGTAAACTTTTATTAGATATGAACCGCAATGTAAGTAACGAACGTGCAAAAACATTACTTGGTTGGGAACCG
ATTGCGACACAAGAAGAAGCAATTTTAGCAGCTGTCGATAGTATGGCTAAGTATCATTTAATATAA

Upstream 100 bases:

>100_bases
AATTCATATAGAATATTAAGCAATATAAGTCTGTTGATATATACAAAATATAATGACTGCTATAATGAGTAATCAATAGA
CACAAAGAGGAGATTATGTG

Downstream 100 bases:

>100_bases
TACAAAACACCGTCCATAAGTAAGTGGCTTTCCTCTTGATAAAAGGGGCATTCACTCAATATGTGACGGTGTTTTATATT
TCTACGTTAACACTATTGCT

Product: oxidoreductase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MNNKVLATGGTGFVGMRIISRLLEQGYDVQTTIRDLSKADKVIKTMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVL
SVASPVFFGKTDDAEVMAKPAIEGIQRILRAAEHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSK
LLAEKAAWDFVENENTTVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQIPLNVVDVRDVAELHILAMTNEQ
ANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVSTKKLPDFILSLGAKFNHQAKEGKLLLDMNRNVSNERAKTLLGWEP
IATQEEAILAAVDSMAKYHLI

Sequences:

>Translated_341_residues
MNNKVLATGGTGFVGMRIISRLLEQGYDVQTTIRDLSKADKVIKTMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVL
SVASPVFFGKTDDAEVMAKPAIEGIQRILRAAEHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSK
LLAEKAAWDFVENENTTVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQIPLNVVDVRDVAELHILAMTNEQ
ANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVSTKKLPDFILSLGAKFNHQAKEGKLLLDMNRNVSNERAKTLLGWEP
IATQEEAILAAVDSMAKYHLI
>Mature_341_residues
MNNKVLATGGTGFVGMRIISRLLEQGYDVQTTIRDLSKADKVIKTMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVL
SVASPVFFGKTDDAEVMAKPAIEGIQRILRAAEHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSK
LLAEKAAWDFVENENTTVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQIPLNVVDVRDVAELHILAMTNEQ
ANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVSTKKLPDFILSLGAKFNHQAKEGKLLLDMNRNVSNERAKTLLGWEP
IATQEEAILAAVDSMAKYHLI

Specific function: Unknown

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI71987463, Length=327, Percent_Identity=34.2507645259939, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6320750, Length=341, Percent_Identity=29.0322580645161, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6321399, Length=335, Percent_Identity=28.3582089552239, Blast_Score=123, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6324421, Length=338, Percent_Identity=27.810650887574, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6321281, Length=318, Percent_Identity=29.2452830188679, Blast_Score=115, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 37749; Mature: 37749

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00152 ATPASE_ALPHA_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKVLATGGTGFVGMRIISRLLEQGYDVQTTIRDLSKADKVIKTMQDNGISTERLMFVE
CCCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
ADLSQDEHWDEAMKDCKYVLSVASPVFFGKTDDAEVMAKPAIEGIQRILRAAEHAGVKRV
CCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
VMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSKLLAEKAAWDFVENENTTVEF
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
ATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQIPLNVVDVRDVAELHILAMTNEQ
EECCEEEEECCCCCCCCCCHHHHHHHHHCCHHHHCCCCCCEEECHHHHHHEEEEEEECCC
ANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVSTKKLPDFILSLGAKFNHQAKEGKLL
CCCCEEEEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
LDMNRNVSNERAKTLLGWEPIATQEEAILAAVDSMAKYHLI
EEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNNKVLATGGTGFVGMRIISRLLEQGYDVQTTIRDLSKADKVIKTMQDNGISTERLMFVE
CCCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
ADLSQDEHWDEAMKDCKYVLSVASPVFFGKTDDAEVMAKPAIEGIQRILRAAEHAGVKRV
CCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
VMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSKLLAEKAAWDFVENENTTVEF
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
ATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQIPLNVVDVRDVAELHILAMTNEQ
EECCEEEEECCCCCCCCCCHHHHHHHHHCCHHHHCCCCCCEEECHHHHHHEEEEEEECCC
ANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVSTKKLPDFILSLGAKFNHQAKEGKLL
CCCCEEEEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
LDMNRNVSNERAKTLLGWEPIATQEEAILAAVDSMAKYHLI
EEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]