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The map label for this gene is cocE [H]

Identifier: 57652331

GI number: 57652331

Start: 2668555

End: 2670237

Strand: Direct

Name: cocE [H]

Synonym: SACOL2612

Alternate gene names: 57652331

Gene position: 2668555-2670237 (Clockwise)

Preceding gene: 57652329

Following gene: 57652335

Centisome position: 94.99

GC content: 36.72

Gene sequence:

>1683_bases
ATGAACCAACATTTACTAGGAAATCCAAAATTAACTGTAACTCATGTCAATGAAGTTAAAGCCGGTATTAACCACATCGT
TGTCGACAGTGTTCAATATGGAAACCAAGAAATGATTATGGAAAAAGATGGCACTGTGGAAATGCGCGATGGCGAAAAAT
TATATATTAATATTTTCAGACCAAATAAAGATGGCAAATTCCCTGTAGTTATGTCTGCAGATACTTACGGTAAAGATAAT
AAGCCTAAAATCACAAATATGGGTGCCCTTTGGCCAACATTAGGTACCATTCCGACATCTAGTTTTACACCTGAAGAATC
ACCAGACCCAGGATTTTGGGTGCCAAATGATTATGTTGTAGTTAAAGTTGCATTACGCGGTAGTGACAAATCCAAAGGCG
TCTTATCTCCATGGTCAAAAAGAGAAGCGGAAGATTATTACGAAGTGATTGAATGGGCAGCAAATCAGTCATGGAGTAAT
GGAAATATCGGGACAAATGGTGTTTCTTATCTTGCGGTGACTCAATGGTGGGTCGCATCATTAAATCCACCACATTTAAA
AGCAATGATTCCTTGGGAAGGCTTAAATGATATGTATAGAGAAGTAGCCTTTCACGGAGGTATACCAGATACTGGCTTTT
ATCGTTTCTGGACTCAAGGTATTTTTGCGAGATGGACAGATAATCCAAATATCGAAGATTTGATTCAAGCACAACAAGAA
CATCCTCTGTTCGATGATTTTTGGAAACAGCGTCAAGTGCCATTATCACAAATTAAAACACCTCTACTAACATGTGCTAG
TTGGTCTACACAAGGTTTGCACAACCGTGGCTCTTTTGAAGGATTTAAACAAGCTGCATCTGAAGAAAAATGGCTATATG
TGCATGGACGTAAAGAGTGGGAAAGTTACTACGCTAGAGAAAATCTCGAACGCCAAAAATCATTCTTTGATTTTTACCTT
AAAGAAGAAAATAACGATTGGAAAGATACGCCTCATGTCATTTATGAAGTTAGAGATCAATTTTATAAAGGCGAATTCAA
ATCAGCGTCAGCGTTCCCTTTACCTAACGCAGAATATACACCATTGTATTTGAATGCTGAAAATCACACATTGAATCATG
CAAAGATTAGTAGCGCGCATGTCGCACAATATGACTCTGAAGATAAACAACAAGATGTAAGTTTTAAATATACGTTTGAC
AAAGATACTGAGTTAGTTGGAAACATGAACTTAAAACTATGGGTAAGCACTAAAGACTCAGATGATATGGATTTATTTGC
AGGTATTAAAAAGTTAGATCGTCGTGGTAATGAAGTTAACTTCCCTGATTTTAATCATATTGAAAATGGTCAAGTAGCTA
CTGGTTGGTTACGCGTATCACATCGTGAATTAGATCAAGAAAAATCCTCAATCGCGCAACCTTGGCATAAACATGAAACA
GAATTAAAGTTGTCACAAGATGAGATTGTACCTGTTGAAATCGAATTGTTACCTTCAGGCACGCTATTTAAACAAGGCGA
AACATTGGAAGTTGTTGTAAAGGGTAGTGAAATTGTAATTGGTAATAGTACTCCTGGCATGAAAACACGTTATGAACATG
AAGAAACCGTAAATAAAGGCATGCACATGATTTATACTGGTGGTAAATATGATTCACAATTAATCATTCCTATCGTTAAT
TGA

Upstream 100 bases:

>100_bases
TTATGTTCATTATCCCACAAATCTCCAACATTGATGATTGGCACACAATGTTTACCTGTTTAATATAGGTGATACAAACA
AACAGAAAAAGGTGATAACA

Downstream 100 bases:

>100_bases
TATGCAGCAATTACGGTCGCTTTTGATTAAAAGTGACATAGTGATAGGACTGTATAACAAGAGAAAGCCACACGCTTGGA
ATCTTAAACCAAGGTGTGGC

Product: CocE/NonD family hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MNQHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEMIMEKDGTVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDN
KPKITNMGALWPTLGTIPTSSFTPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSN
GNIGTNGVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVAFHGGIPDTGFYRFWTQGIFARWTDNPNIEDLIQAQQE
HPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNRGSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYL
KEENNDWKDTPHVIYEVRDQFYKGEFKSASAFPLPNAEYTPLYLNAENHTLNHAKISSAHVAQYDSEDKQQDVSFKYTFD
KDTELVGNMNLKLWVSTKDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENGQVATGWLRVSHRELDQEKSSIAQPWHKHET
ELKLSQDEIVPVEIELLPSGTLFKQGETLEVVVKGSEIVIGNSTPGMKTRYEHEETVNKGMHMIYTGGKYDSQLIIPIVN

Sequences:

>Translated_560_residues
MNQHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEMIMEKDGTVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDN
KPKITNMGALWPTLGTIPTSSFTPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSN
GNIGTNGVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVAFHGGIPDTGFYRFWTQGIFARWTDNPNIEDLIQAQQE
HPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNRGSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYL
KEENNDWKDTPHVIYEVRDQFYKGEFKSASAFPLPNAEYTPLYLNAENHTLNHAKISSAHVAQYDSEDKQQDVSFKYTFD
KDTELVGNMNLKLWVSTKDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENGQVATGWLRVSHRELDQEKSSIAQPWHKHET
ELKLSQDEIVPVEIELLPSGTLFKQGETLEVVVKGSEIVIGNSTPGMKTRYEHEETVNKGMHMIYTGGKYDSQLIIPIVN
>Mature_560_residues
MNQHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEMIMEKDGTVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDN
KPKITNMGALWPTLGTIPTSSFTPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSN
GNIGTNGVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVAFHGGIPDTGFYRFWTQGIFARWTDNPNIEDLIQAQQE
HPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNRGSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYL
KEENNDWKDTPHVIYEVRDQFYKGEFKSASAFPLPNAEYTPLYLNAENHTLNHAKISSAHVAQYDSEDKQQDVSFKYTFD
KDTELVGNMNLKLWVSTKDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENGQVATGWLRVSHRELDQEKSSIAQPWHKHET
ELKLSQDEIVPVEIELLPSGTLFKQGETLEVVVKGSEIVIGNSTPGMKTRYEHEETVNKGMHMIYTGGKYDSQLIIPIVN

Specific function: Hydrolyzes cocaine to benzoate and ecgonine methyl ester, endowing the bacteria with the ability to utilize cocaine as a sole source of carbon and energy for growth, as this bacterium lives in the rhizosphere of coca plants [H]

COG id: COG2936

COG function: function code R; Predicted acyl esterases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha/beta hydrolase family. Carboxylesterase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005674
- InterPro:   IPR008979
- InterPro:   IPR013736
- InterPro:   IPR000383 [H]

Pfam domain/function: PF02129 Peptidase_S15; PF08530 PepX_C [H]

EC number: NA

Molecular weight: Translated: 64249; Mature: 64249

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEMIMEKDGTVEMRDGEKLYINIFR
CCCCCCCCCCEEEEEHHHHHCCCCEEEEECEECCCCEEEEECCCEEEEECCCEEEEEEEC
PNKDGKFPVVMSADTYGKDNKPKITNMGALWPTLGTIPTSSFTPEESPDPGFWVPNDYVV
CCCCCCEEEEEECCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCEECCCCEEE
VKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSNGNIGTNGVSYLAVTQWWVAS
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEHHHHC
LNPPHLKAMIPWEGLNDMYREVAFHGGIPDTGFYRFWTQGIFARWTDNPNIEDLIQAQQE
CCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHCCEEEEECCCCCHHHHHHHHHC
HPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNRGSFEGFKQAASEEKWLYVHGRKEW
CCCHHHHHHHCCCCHHHHCCHHEEECCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCHHH
ESYYARENLERQKSFFDFYLKEENNDWKDTPHVIYEVRDQFYKGEFKSASAFPLPNAEYT
HHHHHHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
PLYLNAENHTLNHAKISSAHVAQYDSEDKQQDVSFKYTFDKDTELVGNMNLKLWVSTKDS
EEEEECCCCCCCCEECCCCHHHHCCCCCCCCCCEEEEEECCCCEEEECCEEEEEEECCCC
DDMDLFAGIKKLDRRGNEVNFPDFNHIENGQVATGWLRVSHRELDQEKSSIAQPWHKHET
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHCCHHCCCC
ELKLSQDEIVPVEIELLPSGTLFKQGETLEVVVKGSEIVIGNSTPGMKTRYEHEETVNKG
EEEECCCCEEEEEEEEECCCCEEECCCEEEEEEECCEEEEECCCCCCCCCCCHHHHHHCC
MHMIYTGGKYDSQLIIPIVN
CEEEEECCCCCCEEEEEECC
>Mature Secondary Structure
MNQHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEMIMEKDGTVEMRDGEKLYINIFR
CCCCCCCCCCEEEEEHHHHHCCCCEEEEECEECCCCEEEEECCCEEEEECCCEEEEEEEC
PNKDGKFPVVMSADTYGKDNKPKITNMGALWPTLGTIPTSSFTPEESPDPGFWVPNDYVV
CCCCCCEEEEEECCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCEECCCCEEE
VKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSNGNIGTNGVSYLAVTQWWVAS
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEHHHHC
LNPPHLKAMIPWEGLNDMYREVAFHGGIPDTGFYRFWTQGIFARWTDNPNIEDLIQAQQE
CCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHCCEEEEECCCCCHHHHHHHHHC
HPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNRGSFEGFKQAASEEKWLYVHGRKEW
CCCHHHHHHHCCCCHHHHCCHHEEECCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCHHH
ESYYARENLERQKSFFDFYLKEENNDWKDTPHVIYEVRDQFYKGEFKSASAFPLPNAEYT
HHHHHHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
PLYLNAENHTLNHAKISSAHVAQYDSEDKQQDVSFKYTFDKDTELVGNMNLKLWVSTKDS
EEEEECCCCCCCCEECCCCHHHHCCCCCCCCCCEEEEEECCCCEEEECCEEEEEEECCCC
DDMDLFAGIKKLDRRGNEVNFPDFNHIENGQVATGWLRVSHRELDQEKSSIAQPWHKHET
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHCCHHCCCC
ELKLSQDEIVPVEIELLPSGTLFKQGETLEVVVKGSEIVIGNSTPGMKTRYEHEETVNKG
EEEECCCCEEEEEEEEECCCCEEECCCEEEEEEECCEEEEECCCCCCCCCCCHHHHHHCC
MHMIYTGGKYDSQLIIPIVN
CEEEEECCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10698749; 11742345; 12369817 [H]