Click here to switch to the map view.

The map label for this gene is mtlA

Identifier: 57652174

GI number: 57652174

Start: 2208813

End: 2210351

Strand: Direct

Name: mtlA

Synonym: SACOL2146

Alternate gene names: 57652174

Gene position: 2208813-2210351 (Clockwise)

Preceding gene: 57652169

Following gene: 57652175

Centisome position: 78.62

GC content: 35.67

Gene sequence:

>1539_bases
ATGTCACAAACTGAAGAGAAAAAAGGAATTGGTCGTCGTGTTCAAGCATTTGGATCATTCTTAAGTAGTATGATCATGCC
AAACATTGGCGCTTTCATCGCTTGGGGTTTTATCGCGGCCATTTTTATAGATAATGGATGGTTACCTAACAAAGATTTAG
CTACTTTAGCAGGACCAATGATTACTTATTTAATCCCATTATTGATCGCATTTAGTGGTGGTCGTTTAATTTATGATTTA
CGTGGTGGTATCATCGCAGCAACAGCAACTATGGGGGTCATCGTTGCATTACCTGATACACCAATGTTACTTGGTGCAAT
GATTATGGGTCCACTTGTTGGTTGGTTAATGAAGAAGACCGACCAATTGATTCAACCTAGAACGCCGCAAGGTTTTGAAA
TGTTATTTAATAACTTTTCTGCTGGTATTTTAGGATTTATCATGACGATTGCAGGATTTAAAATTTTAGCACCACTTATG
AAATTTATTATGCATATTTTATCAGTGGCAGTTGAAGCATTAGTACATGCACACCTACTTCCACTTGTAAGTATTTTAGT
AGAGCCTGCAAAAATTGTATTTTTAAACAATGCGATTAACCATGGTGTATTCACACCGCTTGGTGCAGATCAAGCTGCAA
AAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTT
GGAAAAGGCACTGCAAAAACAACGTCATATGGTGCTGGAATTATCCACTTCTTAGGTGGTATTCATGAAATTTATTTCCC
ATATGTATTAATGCGTCCTTTATTATTTATCGCTGTTATTTTAGGTGGAATGACTGGTGTTGCAACTTATCAAGCAACAG
GATTTGGATTTAAAAGTCCCGCGTCACCAGGTTCATTTATAGTTTACTGTTTAAATGCACCTAGAGGTGAATTCTTGCAC
ATGTTACTCGGTGTCTTCCTTGCTGCACTTGTATCATTCGTTGTAGCTGCTTTAATTATGAAGTTCACTAGAGAACCAAA
GCAGGATTTAGAAGCTGCGACAGCTCAAATGGAAAATACTAAAGGGAAAAAATCAAGCGTTGCTTCTAAGTTAGTATCTT
CTGATAAAAATGTTAATACAGAAGAAAATGCTAGTGGTAATGTTAGTGAAACATCTTCATCAGATGATGATCCTGAAGCG
CTATTGGATAATTACAACACTGAAGATGTTGATGCACACAATTACAATAATATAAATCATGTTATTTTTGCCTGCGATGC
GGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGTAATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTA
CAAATACTGCGATTAATCAATTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAA
CAAACACCAAATGCCATCCATATTTCAGTGGATAATTTCCTTAATTCACCAAGATATGAAGAACTTTTAAATAATCTAAA
AAAAGATGATCAAGCATAA

Upstream 100 bases:

>100_bases
CACATTTATTTTGACAAAGTTGATTTTTGTTTATATTGAGTAACAAGTAACCTCTCTATACACTATATATAGTCACATAT
ATTAAAAAAGAGGTGTAAAC

Downstream 100 bases:

>100_bases
TAATTAAATAAATTAAAAAATGGAGGATACCGCCATGTTATTGAGTACACGTGAAAAAGAAATGATAGCCCTATTGATTA
AGTACCACGGTCAATATATC

Product: PTS system, mannitol-specific IIBC components

Products: NA

Alternate protein names: EIICB-Mtl; EII-Mtl; Mannitol permease IIC component; PTS system mannitol-specific EIIC component; Mannitol-specific phosphotransferase enzyme IIB component; PTS system mannitol-specific EIIB component

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFIDNGWLPNKDLATLAGPMITYLIPLLIAFSGGRLIYDL
RGGIIAATATMGVIVALPDTPMLLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLM
KFIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQSILYTIESNPGPGLGILLAYMIF
GKGTAKTTSYGAGIIHFLGGIHEIYFPYVLMRPLLFIAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLH
MLLGVFLAALVSFVVAALIMKFTREPKQDLEAATAQMENTKGKKSSVASKLVSSDKNVNTEENASGNVSETSSSDDDPEA
LLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGINDITVTNTAINQLPKDAQLVITQKKLTDRAIK
QTPNAIHISVDNFLNSPRYEELLNNLKKDDQA

Sequences:

>Translated_512_residues
MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFIDNGWLPNKDLATLAGPMITYLIPLLIAFSGGRLIYDL
RGGIIAATATMGVIVALPDTPMLLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLM
KFIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQSILYTIESNPGPGLGILLAYMIF
GKGTAKTTSYGAGIIHFLGGIHEIYFPYVLMRPLLFIAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLH
MLLGVFLAALVSFVVAALIMKFTREPKQDLEAATAQMENTKGKKSSVASKLVSSDKNVNTEENASGNVSETSSSDDDPEA
LLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGINDITVTNTAINQLPKDAQLVITQKKLTDRAIK
QTPNAIHISVDNFLNSPRYEELLNNLKKDDQA
>Mature_511_residues
SQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFIDNGWLPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLR
GGIIAATATMGVIVALPDTPMLLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLMK
FIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQSILYTIESNPGPGLGILLAYMIFG
KGTAKTTSYGAGIIHFLGGIHEIYFPYVLMRPLLFIAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHM
LLGVFLAALVSFVVAALIMKFTREPKQDLEAATAQMENTKGKKSSVASKLVSSDKNVNTEENASGNVSETSSSDDDPEAL
LDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGINDITVTNTAINQLPKDAQLVITQKKLTDRAIKQ
TPNAIHISVDNFLNSPRYEELLNNLKKDDQA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2213

COG function: function code G; Phosphotransferase system, mannitol-specific IIBC component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain

Homologues:

Organism=Escherichia coli, GI1790027, Length=495, Percent_Identity=50.9090909090909, Blast_Score=476, Evalue=1e-135,
Organism=Escherichia coli, GI1789301, Length=506, Percent_Identity=42.4901185770751, Blast_Score=406, Evalue=1e-114,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTMCB_STAAC (Q5HE48)

Other databases:

- EMBL:   CP000046
- RefSeq:   YP_186959.1
- ProteinModelPortal:   Q5HE48
- SMR:   Q5HE48
- STRING:   Q5HE48
- EnsemblBacteria:   EBSTAT00000009586
- GeneID:   3237438
- GenomeReviews:   CP000046_GR
- KEGG:   sac:SACOL2146
- TIGR:   SACOL2146
- eggNOG:   COG2213
- GeneTree:   EBGT00050000024561
- HOGENOM:   HBG296680
- OMA:   GMIMPNI
- ProtClustDB:   CLSK2460866
- BioCyc:   SAUR93062:SACOL2146-MONOMER
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004718
- TIGRFAMs:   TIGR00851

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB

EC number: =2.7.1.69

Molecular weight: Translated: 55096; Mature: 54965

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: PS51099 PTS_EIIB_TYPE_2; PS51104 PTS_EIIC_TYPE_2

Important sites: ACT_SITE 425-425

Signals:

None

Transmembrane regions:

HASH(0xbb19c6c)-; HASH(0xfcaf018)-; HASH(0xb71e014)-; HASH(0xee502ec)-; HASH(0xfdb8f78)-; HASH(0xfd628c0)-; HASH(0xfa4f458)-; HASH(0xf69dec8)-; HASH(0xf834aa4)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFIDNGWLPNKDLATLAGPM
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
ITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPMLLGAMIMGPLVGWLMKKT
HHHHHHHHHHHCCCCEEEEECCCEEEEHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
DQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLMKFIMHILSVAVEALVHAHLL
HHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQSILYTIESNPGPGLGILLAYMIF
HHHHHHHCCHHEEEEECCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCHHHHHHHHHH
GKGTAKTTSYGAGIIHFLGGIHEIYFPYVLMRPLLFIAVILGGMTGVATYQATGFGFKSP
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEECCCCCCCC
ASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFVVAALIMKFTREPKQDLEAATAQMENT
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
KGKKSSVASKLVSSDKNVNTEENASGNVSETSSSDDDPEALLDNYNTEDVDAHNYNNINH
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCE
VIFACDAGMGSSAMGASMLRNKFKKAGINDITVTNTAINQLPKDAQLVITQKKLTDRAIK
EEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHCCCCHHEEEEHHHHHHHHHH
QTPNAIHISVDNFLNSPRYEELLNNLKKDDQA
CCCCEEEEEHHHHHCCCCHHHHHHHHHCCCCH
>Mature Secondary Structure 
SQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFIDNGWLPNKDLATLAGPM
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
ITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPMLLGAMIMGPLVGWLMKKT
HHHHHHHHHHHCCCCEEEEECCCEEEEHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
DQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLMKFIMHILSVAVEALVHAHLL
HHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQSILYTIESNPGPGLGILLAYMIF
HHHHHHHCCHHEEEEECCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCHHHHHHHHHH
GKGTAKTTSYGAGIIHFLGGIHEIYFPYVLMRPLLFIAVILGGMTGVATYQATGFGFKSP
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEECCCCCCCC
ASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFVVAALIMKFTREPKQDLEAATAQMENT
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
KGKKSSVASKLVSSDKNVNTEENASGNVSETSSSDDDPEALLDNYNTEDVDAHNYNNINH
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCE
VIFACDAGMGSSAMGASMLRNKFKKAGINDITVTNTAINQLPKDAQLVITQKKLTDRAIK
EEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHCCCCHHEEEEHHHHHHHHHH
QTPNAIHISVDNFLNSPRYEELLNNLKKDDQA
CCCCEEEEEHHHHHCCCCHHHHHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA