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The map label for this gene is putP

Identifier: 57652123

GI number: 57652123

Start: 2023853

End: 2025391

Strand: Direct

Name: putP

Synonym: SACOL1963

Alternate gene names: 57652123

Gene position: 2023853-2025391 (Clockwise)

Preceding gene: 57652115

Following gene: 57650660

Centisome position: 72.04

GC content: 35.93

Gene sequence:

>1539_bases
ATGCTTACAATGGGGACAGCATTAAGTCAACAAGTAGATGCCAATTGGCAAACTTATATTATGATTGCCGTCTACTTCTT
GATACTAATCGTTATTGGCTTTTACGGTTACAAGCAAGCAACTGGTAACCTAAGCGAGTACATGTTAGGTGGACGTAGTA
TTGGACCGTATATTACTGCATTATCAGCTGGAGCTTCAGATATGAGTGGATGGATGATTATGGGGCTACCTGGTTCTGTC
TATAGCACTGGTCTATCAGCTATGTGGATTACAATCGGTTTAACATTAGGTGCTTATATAAATTACTTTGTTGTTGCTCC
TAGACTTCGTGTTTATACCGAATTAGCTGGAGATGCAATTACATTACCAGATTTCTTTAAAAATCGTTTAAACGATAAAA
ATAATGTGTTAAAGATTATTTCTGGATTGATTATCGTAGTATTCTTTACATTATATACACATTCTGGTTTCGTATCTGGT
GGTAAACTATTTGAAAGTGCTTTTGGATTAGATTATCATTTCGGTTTAATATTAGTTGCTTTCATTGTCATTTTCTATAC
TTTCTTTGGTGGATATTTAGCTGTATCAATTACAGATTTCTTCCAAGGTGTCATTATGTTAATTGCGATGGTTATGGTCC
CTATTGTTGCTATGATGAATTTAAACGGCTGGGGAACGTTTCATGATGTAGCAGCTATGAAACCTACAAATTTAAATTTA
TTTAAAGGGTTATCATTTATAGGAATTATCTCTCTATTTTCATGGGGATTAGGTTATTTCGGTCAACCTCATATCATTGT
AAGGTTTATGTCTATTAAATCACACAAGATGCTACCTAAAGCTAGACGTTTAGGTATTAGCTGGATGGCTGTTGGTTTAT
TAGGCGCTGTGGCTGTTGGTTTAACAGGTATTGCATTCGTACCTGCTTATCATATTAAACTAGAAGATCCTGAGACATTA
TTCATCGTGATGAGTCAAGTACTCTTCCATCCTCTTGTAGGTGGTTTCTTACTTGCTGCGATTCTAGCTGCAATTATGAG
CACGATTTCTTCACAATTACTTGTAACATCTAGTTCACTAACGGAAGACTTTTATAAATTAATTCGTGGTGAAGAAAAAG
CTAAAACGCACCAAAAAGAATTTGTTATGATTGGAAGATTATCTGTATTAGTTGTAGCAATTGTTGCCATCGCGATTGCA
TGGAATCCAAACGACACAATTCTAAACTTAGTAGGTAACGCTTGGGCCGGATTTGGTGCATCGTTCAGTCCACTTGTGCT
ATTTGCACTTTACTGGAAAGGTTTGACACGTGCCGGTGCTGTAAGTGGAATGGTTTCAGGTGCCTTAGTCGTTATCGTTT
GGATTGCGTGGATTAAACCATTGGCACATATCAACGAAATATTCGGCTTATATGAAATTATTCCTGGATTTATTGTAAGT
GTAATCGTTACATATGTTGTAAGTAAACTTACTAAAAAACCTGGTGCATTTGTTGAAACTGACTTAAACAAAGTTCGTGA
CATCGTTAGAGAAAAATAA

Upstream 100 bases:

>100_bases
AAAAGAATAAATTTAATTTTACTACTTTTCTAAACATTTATCTTTATGTATAATGTTTTCAAGTAACTAAATTATAAATT
AAATAAAGGGAGTGTTTATC

Downstream 100 bases:

>100_bases
TTCATAAGTCTTAACAAATTAAAAAGGTACTAATGTTAATCAAAATTATGACTAACATTGGTACCTTTTTATTATCTTTA
ATTTACAATTAATTACTGTA

Product: high affinity proline permease

Products: NA

Alternate protein names: Proline permease

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNLSEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSV
YSTGLSAMWITIGLTLGAYINYFVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAMMNLNGWGTFHDVAAMKPTNLNL
FKGLSFIGIISLFSWGLGYFGQPHIIVRFMSIKSHKMLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETL
FIVMSQVLFHPLVGGFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVVAIVAIAIA
WNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALVVIVWIAWIKPLAHINEIFGLYEIIPGFIVS
VIVTYVVSKLTKKPGAFVETDLNKVRDIVREK

Sequences:

>Translated_512_residues
MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNLSEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSV
YSTGLSAMWITIGLTLGAYINYFVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAMMNLNGWGTFHDVAAMKPTNLNL
FKGLSFIGIISLFSWGLGYFGQPHIIVRFMSIKSHKMLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETL
FIVMSQVLFHPLVGGFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVVAIVAIAIA
WNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALVVIVWIAWIKPLAHINEIFGLYEIIPGFIVS
VIVTYVVSKLTKKPGAFVETDLNKVRDIVREK
>Mature_512_residues
MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNLSEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSV
YSTGLSAMWITIGLTLGAYINYFVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAMMNLNGWGTFHDVAAMKPTNLNL
FKGLSFIGIISLFSWGLGYFGQPHIIVRFMSIKSHKMLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETL
FIVMSQVLFHPLVGGFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVVAIVAIAIA
WNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALVVIVWIAWIKPLAHINEIFGLYEIIPGFIVS
VIVTYVVSKLTKKPGAFVETDLNKVRDIVREK

Specific function: Catalyzes the sodium-dependent uptake of extracellular L-proline. Since most S.aureus strains are L- proline auxotrophs, this transporter may aid the bacterial persistence during an infection of tissues with low proline concentrations (By similarity)

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family

Homologues:

Organism=Homo sapiens, GI310128183, Length=512, Percent_Identity=73.6328125, Blast_Score=760, Evalue=0.0,
Organism=Escherichia coli, GI1787251, Length=494, Percent_Identity=50.2024291497976, Blast_Score=443, Evalue=1e-125,
Organism=Escherichia coli, GI87082237, Length=475, Percent_Identity=29.2631578947368, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1790503, Length=480, Percent_Identity=25.4166666666667, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1790113, Length=393, Percent_Identity=24.1730279898219, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24645928, Length=374, Percent_Identity=26.2032085561497, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24640370, Length=401, Percent_Identity=25.9351620947631, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24648033, Length=401, Percent_Identity=24.6882793017456, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356865, Length=401, Percent_Identity=24.6882793017456, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI221459584, Length=356, Percent_Identity=23.0337078651685, Blast_Score=69, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUTP_STAA1 (A7X430)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442477.1
- STRING:   A7X430
- EnsemblBacteria:   EBSTAT00000004667
- GeneID:   5560501
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1887
- eggNOG:   COG0591
- GeneTree:   EBGT00050000025067
- HOGENOM:   HBG499520
- OMA:   FRRNASD
- ProtClustDB:   CLSK885638
- BioCyc:   SAUR418127:SAHV_1887-MONOMER
- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR019900
- PANTHER:   PTHR11819
- TIGRFAMs:   TIGR02121
- TIGRFAMs:   TIGR00813

Pfam domain/function: PF00474 SSF

EC number: NA

Molecular weight: Translated: 55976; Mature: 55976

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS00456 NA_SOLUT_SYMP_1; PS00457 NA_SOLUT_SYMP_2; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xe5461d4)-; HASH(0xf6d1020)-; HASH(0xef62908)-; HASH(0xf6a0288)-; HASH(0xfab6c14)-; HASH(0xf1add1c)-; HASH(0xfab10ec)-; HASH(0xfab69b0)-; HASH(0xfa27ba0)-; HASH(0xfab692c)-; HASH(0xec5ec84)-; HASH(0xf5a7aa8)-; HASH(0xe3d5738)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCH
>Mature Secondary Structure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HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA