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The map label for this gene is gatA [H]

Identifier: 57652121

GI number: 57652121

Start: 2021724

End: 2023181

Strand: Reverse

Name: gatA [H]

Synonym: SACOL1961

Alternate gene names: 57652121

Gene position: 2023181-2021724 (Counterclockwise)

Preceding gene: 57652122

Following gene: 57652120

Centisome position: 72.01

GC content: 34.09

Gene sequence:

>1458_bases
ATGAGCATTCGCTACGAATCGGTTGAGAATTTATTAACTTTAATAAAAGACAAAAAAATCAAACCATCTGATGTTGTTAA
AGATATATATGATGCAATTGAAGAGACTGATCCAACAATTAAGTCTTTTCTAGCGCTGGATAAAGAAAATGCAATCAAAA
AAGCGCAAGAATTGGATGAATTACAAGCAAAAGATCAAATGGATGGCAAATTATTTGGTATTCCAATGGGTATAAAAGAT
AACATTATTACAAACGGATTAGAAACAACATGTGCAAGTAAAATGTTAGAAGGTTTTGTGCCAATTTACGAATCTACTGT
AATGGAAAAACTACATAATGAAAATGCCGTTTTAATCGGTAAATTAAATATGGATGAGTTTGCAATGGGTGGTTCAACAG
AAACATCTTATTTCAAAAAAACAGTTAACCCATTTGACCATAAAGCAGTGCCAGGTGGTTCATCAGGTGGATCTGCAGCA
GCAGTTGCAGCTGGCTTAGTACCATTTAGCTTAGGTTCAGACACAGGTGGTTCAATTAGACAACCGGCTGCATATTGTGG
CGTTGTCGGTATGAAACCAACATACGGTCGTGTATCTCGATTTGGATTAGTTGCTTTTGCATCTTCATTAGACCAAATTG
GTCCATTGACTCGAAATGTAAAAGATAATGCAATCGTATTAGAAGCTATTTCTGGTGCAGATGTTAATGACTCTACAAGT
GCACCAGTTGATGATGTAGACTTTACATCTGAAATTGGTAAAGATATTAAAGGATTAAAAGTTGCATTACCTAAAGAATA
CTTAGGTGAAGGTGTAGCTGATGACGTAAAAGAAGCAGTTCAAAACGCTGTAGAAACTTTAAAATCTTTAGGTGCTGTCG
TTGAGGAAGTATCATTGCCAAATACTAAATTTGGTATTCCATCATATTACGTGATTGCATCATCAGAAGCTTCGTCAAAC
CTTTCTCGTTTTGACGGAATTCGTTATGGTTATCATTCTAAAGAAGCTCATTCATTAGAAGAATTATATAAAATGTCAAG
ATCTGAAGGTTTCGGTAAAGAAGTAAAACGTCGTATTTTCTTAGGTACATTTGCATTAAGTTCAGGTTACTATGATGCTT
ACTATAAAAAATCTCAAAAAGTTAGAACATTGATTAAAAATGACTTTGATAAAGTATTCGAAAATTATGATGTAGTAGTT
GGTCCAACAGCGCCTACAACTGCGTTTAATTTAGGTGAAGAAATTGATGATCCATTAACAATGTATGCCAATGATTTATT
AACAACACCAGTAAACTTAGCTGGATTACCTGGTATTTCTGTTCCTTGTGGACAATCAAATGGCCGACCAATCGGTTTAC
AGTTCATTGGTAAACCATTCGATGAAAAAACGTTATATCGTGTCGCTTATCAATATGAAACACAATACAATTTACATGAC
GTTTATGAAAAATTATAA

Upstream 100 bases:

>100_bases
ATTAAAGGTATTCCACAAGAATTAGCTTTGAAAAATGCCAAAGAAACAGAAGATGGACAATTTAAAGTGCCTACAATCAT
GAATGAGGAGGACGCGTAAG

Downstream 100 bases:

>100_bases
GGAGTGGAAATCATGCATTTTGAAACAGTTATAGGACTTGAAGTTCACGTAGAGTTAAAAACGGACTCAAAAATGTTTTC
TCCATCACCAGCGCATTTTG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFLALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKD
NIITNGLETTCASKMLEGFVPIYESTVMEKLHNENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAA
AVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTRNVKDNAIVLEAISGADVNDSTS
APVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDVKEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSN
LSRFDGIRYGYHSKEAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKVFENYDVVV
GPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRPIGLQFIGKPFDEKTLYRVAYQYETQYNLHD
VYEKL

Sequences:

>Translated_485_residues
MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFLALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKD
NIITNGLETTCASKMLEGFVPIYESTVMEKLHNENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAA
AVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTRNVKDNAIVLEAISGADVNDSTS
APVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDVKEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSN
LSRFDGIRYGYHSKEAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKVFENYDVVV
GPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRPIGLQFIGKPFDEKTLYRVAYQYETQYNLHD
VYEKL
>Mature_484_residues
SIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFLALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDN
IITNGLETTCASKMLEGFVPIYESTVMEKLHNENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAAA
VAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTRNVKDNAIVLEAISGADVNDSTSA
PVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDVKEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNL
SRFDGIRYGYHSKEAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKVFENYDVVVG
PTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRPIGLQFIGKPFDEKTLYRVAYQYETQYNLHDV
YEKL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=496, Percent_Identity=38.9112903225806, Blast_Score=326, Evalue=3e-89,
Organism=Homo sapiens, GI195972892, Length=484, Percent_Identity=25.2066115702479, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI166795287, Length=164, Percent_Identity=34.1463414634146, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=429, Percent_Identity=37.995337995338, Blast_Score=288, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI17537465, Length=489, Percent_Identity=23.9263803680982, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17556264, Length=265, Percent_Identity=30.5660377358491, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=231, Percent_Identity=30.3030303030303, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=320, Percent_Identity=26.25, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=270, Percent_Identity=30.3703703703704, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17556278, Length=270, Percent_Identity=30.3703703703704, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17538254, Length=255, Percent_Identity=29.0196078431373, Blast_Score=65, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=416, Percent_Identity=37.5, Blast_Score=243, Evalue=5e-65,
Organism=Saccharomyces cerevisiae, GI6319685, Length=451, Percent_Identity=31.4855875831486, Blast_Score=135, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24648113, Length=488, Percent_Identity=39.5491803278689, Blast_Score=311, Evalue=8e-85,
Organism=Drosophila melanogaster, GI24644968, Length=504, Percent_Identity=26.5873015873016, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24652985, Length=505, Percent_Identity=24.5544554455446, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19922090, Length=505, Percent_Identity=24.5544554455446, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24652981, Length=505, Percent_Identity=24.5544554455446, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24652983, Length=505, Percent_Identity=24.5544554455446, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161078093, Length=507, Percent_Identity=26.0355029585799, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI45550774, Length=234, Percent_Identity=30.7692307692308, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648435, Length=234, Percent_Identity=30.7692307692308, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648437, Length=234, Percent_Identity=30.7692307692308, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21356731, Length=228, Percent_Identity=28.5087719298246, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648441, Length=166, Percent_Identity=33.1325301204819, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648439, Length=166, Percent_Identity=33.1325301204819, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52821; Mature: 52690

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFLALDKENAIKKAQELDE
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHH
LQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPIYESTVMEKLHNENAVLIG
HHHHHCCCCEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
KLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAAAVAAGLVPFSLGSDTGGSIR
ECCHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCEEECCCCCCCCCC
QPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTRNVKDNAIVLEAISGADVNDSTS
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHCCCCCCEEEEEECCCCCCCCCCC
APVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDVKEAVQNAVETLKSLGAVVEEVSLP
CCCCCCHHHHHHCCCCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSKEAHSLEELYKMSRSEGFGKEVKRRIF
CCCCCCCCEEEEECCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
LGTFALSSGYYDAYYKKSQKVRTLIKNDFDKVFENYDVVVGPTAPTTAFNLGEEIDDPLT
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHCCHHCCCHHH
MYANDLLTTPVNLAGLPGISVPCGQSNGRPIGLQFIGKPFDEKTLYRVAYQYETQYNLHD
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEHEECCCCCCHHHHHHHHHHHCCCCCHHH
VYEKL
HHHHC
>Mature Secondary Structure 
SIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFLALDKENAIKKAQELDE
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHH
LQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPIYESTVMEKLHNENAVLIG
HHHHHCCCCEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
KLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAAAVAAGLVPFSLGSDTGGSIR
ECCHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCEEECCCCCCCCCC
QPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTRNVKDNAIVLEAISGADVNDSTS
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHCCCCCCEEEEEECCCCCCCCCCC
APVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDVKEAVQNAVETLKSLGAVVEEVSLP
CCCCCCHHHHHHCCCCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSKEAHSLEELYKMSRSEGFGKEVKRRIF
CCCCCCCCEEEEECCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
LGTFALSSGYYDAYYKKSQKVRTLIKNDFDKVFENYDVVVGPTAPTTAFNLGEEIDDPLT
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHCCHHCCCHHH
MYANDLLTTPVNLAGLPGISVPCGQSNGRPIGLQFIGKPFDEKTLYRVAYQYETQYNLHD
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEHEECCCCCCHHHHHHHHHHHCCCCCHHH
VYEKL
HHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA