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The map label for this gene is leuS [H]

Identifier: 57652056

GI number: 57652056

Start: 1862816

End: 1865233

Strand: Reverse

Name: leuS [H]

Synonym: SACOL1808

Alternate gene names: 57652056

Gene position: 1865233-1862816 (Counterclockwise)

Preceding gene: 57652057

Following gene: 57652055

Centisome position: 66.39

GC content: 34.41

Gene sequence:

>2418_bases
GTGTTGAATTACAACCACAATCAAATTGAAAAGAAATGGCAAGACTATTGGGACGAAAATAAAACATTTAAAACAAATGA
TAACTTAGGTCAAAAGAAATTTTATGCTTTAGACATGTTTCCATATCCATCAGGTGCTGGTTTACATGTTGGACATCCTG
AGGGCTATACAGCAACAGATATCATTTCAAGATATAAAAGAATGCAAGGATATAATGTATTACATCCGATGGGGTGGGAT
GCATTCGGATTACCAGCAGAGCAATATGCTTTAGACACTGGCAACGACCCACGTGAATTTACAAAGAAAAATATCCAAAC
TTTTAAACGACAAATTAAAGAATTAGGGTTCAGTTATGATTGGGATCGTGAAGTTAATACAACAGATCCAGAATACTATA
AATGGACACAGTGGATTTTCATACAGTTATATAACAAAGGTTTAGCATACGTTGATGAAGTTGCAGTTAACTGGTGTCCA
GCATTAGGCACTGTTTTATCTAACGAAGAAGTGATTGATGGTGTCTCTGAACGTGGTGGACATCCAGTTTATCGTAAGCC
GATGAAACAATGGGTACTTAAAATCACAGAATATGCAGATCAATTATTAGCAGATTTAGATGATTTAGATTGGCCTGAGT
CTTTAAAAGATATGCAGCGCAATTGGATTGGACGTTCTGAAGGGGCCAAAGTTTCATTTGATGTAGATAATACGGAAGGA
AAAGTAGAAGTATTTACGACTAGACCAGATACAATCTATGGTGCATCATTCTTAGTCTTAAGTCCTGAACATGCATTAGT
TAATTCAATTACAACAGATGAATATAAAGAAAAAGTAAAAGCTTATCAAACAGAAGCTTCTAAAAAGTCAGATTTAGAAC
GTACAGATTTAGCAAAAGATAAATCAGGTGTATTTACTGGTGCATATGCAACTAATCCTTTATCTGGTGAAAAAGTACAA
ATTTGGATTGCTGATTATGTATTATCAACATATGGTACTGGAGCAATTATGGCAGTACCAGCGCATGATGACAGAGATTA
TGAATTTGCTAAAAAGTTTGATTTGCCAATCATTGAAGTCATCGAAGGTGGAAATGTTGAAGAAGCAGCATACACTGGTG
AAGGTAAACATATTAATTCTGGTGAACTTGATGGTTTAGAAAATGAAGCGGCAATTACTAAAGCTATTCAATTATTAGAG
CAAAAAGGTGCTGGCGAAAAGAAAGTTAATTACAAATTAAGAGATTGGTTATTCAGTCGTCAGCGTTATTGGGGCGAACC
AATTCCTGTCATTCATTGGGAAGATGGAACAATGACAACTGTTCCTGAAGAAGAGCTACCATTGTTGTTACCTGAAACAG
ATGAAATCAAGCCATCAGGGACTGGTGAGTCTCCACTAGCTAATATTGATTCATTTGTAAATGTTGTAGATGAAAAAACA
GGTATGAAAGGACGTCGTGAAACAAATACAATGCCACAATGGGCAGGTAGTTGTTGGTATTATTTACGTTACATCGATCC
TAAAAATGAAAATATGTTAGCAGATCCTGAAAAATTAAAACATTGGTTACCTGTTGATTTATATATCGGTGGAGTAGAAC
ATGCGGTTCTTCACTTATTATATGCAAGATTTTGGCATAAAGTCCTTTATGATTTGGCTATCGTACCTACTAAAGAACCT
TTCCAAAAATTATTTAACCAAGGTATGATTTTAGGAGAAGGTAATGAGAAGATGAGTAAATCTAAAGGAAATGTAATCAA
TCCTGATGATATAGTACAGTCTCATGGTGCAGATACTTTGCGTCTTTACGAAATGTTTATGGGACCTTTAGATGCTGCAA
TTGCATGGAGTGAAAAAGGATTAGATGGGTCTCGTCGATTCTTAGATCGCGTATGGCGTTTAATGGTAAATGAAGATGGG
ACATTGAGTTCAAAAATTGTAACTACAAATAATAAATCTTTAGATAAAGTTTATAACCAAACTGTTAAAAAGGTAACAGA
AGACTTTGAAACATTAGGATTTAATACTGCTATTAGTCAATTAATGGTATTTATTAATGAGTGTTATAAAGTTGATGAAG
TTTATAAACCTTACATTGAAGGCTTCGTTAAAATGTTAGCACCTATTGCACCACATATCGGTGAAGAATTATGGTCAAAA
TTAGGACATGAAGAGTCTATTACGTACCAACCTTGGCCAACTTATGACGAAGCACTACTTGTAGATGATGAAGTAGAAAT
CGTTGTTCAAGTGAATGGTAAATTGAGAGCTAAAATTAAAATTGCTAAAGATACATCAAAAGAAGAAATGCAAGAAATTG
CCTTATCTAATGACAATGTTAAAGCGAGTATTGAAGGTAAAGACATCATGAAAGTCATCGCTGTTCCTCAAAAATTAGTC
AATATTGTAGCTAAATAA

Upstream 100 bases:

>100_bases
GAGTGCACAATTTTTGTGAAATAGGGTGGTAACGCGGCAAATGTCGTCCCTATGTAAATAGAATAGTTAGAGGTGTCTTT
TTTATTGAATAGGAGGAAAT

Downstream 100 bases:

>100_bases
TGTTTTAAGGAGGACTTTGAAATGAAGTCAATTACTACAGATGAATTAAAAAATAAACTTTTAGAATCTAAACCAGTTCA
AATTGTTGATGTTCGTACTG

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 805; Mature: 805

Protein sequence:

>805_residues
MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPSGAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWD
AFGLPAEQYALDTGNDPREFTKKNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCP
ALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDMQRNWIGRSEGAKVSFDVDNTEG
KVEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYKEKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYATNPLSGEKVQ
IWIADYVLSTYGTGAIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAITKAIQLLE
QKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLLPETDEIKPSGTGESPLANIDSFVNVVDEKT
GMKGRRETNTMPQWAGSCWYYLRYIDPKNENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLAIVPTKEP
FQKLFNQGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGSRRFLDRVWRLMVNEDG
TLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLMVFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSK
LGHEESITYQPWPTYDEALLVDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQKLV
NIVAK

Sequences:

>Translated_805_residues
MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPSGAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWD
AFGLPAEQYALDTGNDPREFTKKNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCP
ALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDMQRNWIGRSEGAKVSFDVDNTEG
KVEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYKEKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYATNPLSGEKVQ
IWIADYVLSTYGTGAIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAITKAIQLLE
QKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLLPETDEIKPSGTGESPLANIDSFVNVVDEKT
GMKGRRETNTMPQWAGSCWYYLRYIDPKNENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLAIVPTKEP
FQKLFNQGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGSRRFLDRVWRLMVNEDG
TLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLMVFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSK
LGHEESITYQPWPTYDEALLVDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQKLV
NIVAK
>Mature_805_residues
MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPSGAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWD
AFGLPAEQYALDTGNDPREFTKKNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCP
ALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDMQRNWIGRSEGAKVSFDVDNTEG
KVEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYKEKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYATNPLSGEKVQ
IWIADYVLSTYGTGAIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAITKAIQLLE
QKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLLPETDEIKPSGTGESPLANIDSFVNVVDEKT
GMKGRRETNTMPQWAGSCWYYLRYIDPKNENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLAIVPTKEP
FQKLFNQGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGSRRFLDRVWRLMVNEDG
TLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLMVFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSK
LGHEESITYQPWPTYDEALLVDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQKLV
NIVAK

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=870, Percent_Identity=33.5632183908046, Blast_Score=457, Evalue=1e-128,
Organism=Homo sapiens, GI94721239, Length=188, Percent_Identity=29.2553191489362, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI94721241, Length=188, Percent_Identity=29.2553191489362, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI5454158, Length=192, Percent_Identity=26.0416666666667, Blast_Score=69, Evalue=3e-11,
Organism=Escherichia coli, GI1786861, Length=862, Percent_Identity=42.1113689095128, Blast_Score=677, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=888, Percent_Identity=22.4099099099099, Blast_Score=140, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI71997510, Length=281, Percent_Identity=39.1459074733096, Blast_Score=197, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI71997517, Length=274, Percent_Identity=39.7810218978102, Blast_Score=196, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI212645227, Length=345, Percent_Identity=29.5652173913043, Blast_Score=148, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17510661, Length=455, Percent_Identity=23.7362637362637, Blast_Score=88, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17554638, Length=301, Percent_Identity=27.2425249169435, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323414, Length=810, Percent_Identity=37.037037037037, Blast_Score=464, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6321531, Length=385, Percent_Identity=25.4545454545455, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6319395, Length=180, Percent_Identity=30.5555555555556, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21355409, Length=862, Percent_Identity=32.1345707656613, Blast_Score=397, Evalue=1e-110,
Organism=Drosophila melanogaster, GI28574730, Length=174, Percent_Identity=29.3103448275862, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24668547, Length=174, Percent_Identity=29.3103448275862, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24668543, Length=174, Percent_Identity=29.3103448275862, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 91787; Mature: 91787

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPSGAGLHVGHPEGYTATD
CCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCEEEEEEECCCCCCCCEECCCCCCCCHHH
IISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTKKNIQTFKRQIKELGFSYD
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
WDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCPALGTVLSNEEVIDGVSERGG
CCCCCCCCCCCHHHHHHHHEEHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHCHHHCCC
HPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDMQRNWIGRSEGAKVSFDVDNTEG
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCC
KVEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYKEKVKAYQTEASKKSDLERTDLAKD
EEEEEEECCCCEECCEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCC
KSGVFTGAYATNPLSGEKVQIWIADYVLSTYGTGAIMAVPAHDDRDYEFAKKFDLPIIEV
CCCEEEECCCCCCCCCCEEEEEEHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCCCEEEE
IEGGNVEEAAYTGEGKHINSGELDGLENEAAITKAIQLLEQKGAGEKKVNYKLRDWLFSR
ECCCCCCHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
QRYWGEPIPVIHWEDGTMTTVPEEELPLLLPETDEIKPSGTGESPLANIDSFVNVVDEKT
HHHCCCCCCEEEECCCCEECCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
GMKGRRETNTMPQWAGSCWYYLRYIDPKNENMLADPEKLKHWLPVDLYIGGVEHAVLHLL
CCCCCCCCCCCCHHCCCEEEEEEEECCCCCCCCCCHHHHHCCCCEEEEECCHHHHHHHHH
YARFWHKVLYDLAIVPTKEPFQKLFNQGMILGEGNEKMSKSKGNVINPDDIVQSHGADTL
HHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEECCCHHHHHCCCCCCCHHHHHHHCCCCHH
RLYEMFMGPLDAAIAWSEKGLDGSRRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQ
HHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHH
TVKKVTEDFETLGFNTAISQLMVFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSK
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGHEESITYQPWPTYDEALLVDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNV
CCCCCCCCCCCCCCCCCEEEECCCEEEEEEECCCEEEEEEEECCCCHHHHHHHHHCCCCE
KASIEGKDIMKVIAVPQKLVNIVAK
EEEECHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPSGAGLHVGHPEGYTATD
CCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCEEEEEEECCCCCCCCEECCCCCCCCHHH
IISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTKKNIQTFKRQIKELGFSYD
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
WDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCPALGTVLSNEEVIDGVSERGG
CCCCCCCCCCCHHHHHHHHEEHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHCHHHCCC
HPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDMQRNWIGRSEGAKVSFDVDNTEG
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCC
KVEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYKEKVKAYQTEASKKSDLERTDLAKD
EEEEEEECCCCEECCEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCC
KSGVFTGAYATNPLSGEKVQIWIADYVLSTYGTGAIMAVPAHDDRDYEFAKKFDLPIIEV
CCCEEEECCCCCCCCCCEEEEEEHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCCCEEEE
IEGGNVEEAAYTGEGKHINSGELDGLENEAAITKAIQLLEQKGAGEKKVNYKLRDWLFSR
ECCCCCCHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
QRYWGEPIPVIHWEDGTMTTVPEEELPLLLPETDEIKPSGTGESPLANIDSFVNVVDEKT
HHHCCCCCCEEEECCCCEECCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
GMKGRRETNTMPQWAGSCWYYLRYIDPKNENMLADPEKLKHWLPVDLYIGGVEHAVLHLL
CCCCCCCCCCCCHHCCCEEEEEEEECCCCCCCCCCHHHHHCCCCEEEEECCHHHHHHHHH
YARFWHKVLYDLAIVPTKEPFQKLFNQGMILGEGNEKMSKSKGNVINPDDIVQSHGADTL
HHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEECCCHHHHHCCCCCCCHHHHHHHCCCCHH
RLYEMFMGPLDAAIAWSEKGLDGSRRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQ
HHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHH
TVKKVTEDFETLGFNTAISQLMVFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSK
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGHEESITYQPWPTYDEALLVDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNV
CCCCCCCCCCCCCCCCCEEEECCCEEEEEEECCCEEEEEEEECCCCHHHHHHHHHCCCCE
KASIEGKDIMKVIAVPQKLVNIVAK
EEEECHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12044378 [H]