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The map label for this gene is ytzG [H]

Identifier: 57652051

GI number: 57652051

Start: 1851452

End: 1852147

Strand: Reverse

Name: ytzG [H]

Synonym: SACOL1803

Alternate gene names: 57652051

Gene position: 1852147-1851452 (Counterclockwise)

Preceding gene: 57652052

Following gene: 57652050

Centisome position: 65.93

GC content: 29.74

Gene sequence:

>696_bases
ATGAGAATAGATAAATTTTTAGCAAATATGGGCGTCGGTACACGTAATGAGGTCAAACAGTTACTTAAAAAAGGCCTTGT
AAATGTCAACGAACAAGTAATTAAATCACCAAAAACACATATAGAACCAGAAAACGATAAGATAACAGTTCGTGGAGAAT
TAATAGAATACATTGAAAATGTCTATATTATGTTAAATAAGCCAAAAGGTTATATATCAGCAACTGAAGATCATCATTCA
AAAACCGTTATTGATTTAATACCTGAATACCAACATCTAAATATTTTTCCGGTTGGTCGTCTTGATAAAGATACAGAAGG
GCTTTTATTGATAACGAATGATGGTGATTTTAATCATGAATTAATGAGTCCAAACAAACATGTTTCTAAAAAATATGAAG
TTATTTCAGCAAATCCTATCACTGAAGACGACATTCAAGCTTTTAAAGAGGGTGTAACACTGACTGATGGCAAGGTTAAG
CCTGCAATATTAACTTATATTGATAATCAAACGTCACACGTAACAATTTATGAAGGAAAATATCATCAAGTTAAACGAAT
GTTTCATAGCATTCAAAATGAGGTATTACATTTAAGACGTATCAAAATTGCTGATTTAGAATTGGACAGCAATTTAGATT
CAGGAGAATATCGTTTATTAACTGAAAATGATTTTGACAAATTAAATTATAAATAA

Upstream 100 bases:

>100_bases
GTATTCTAATTTACGGTACAATCACAATTAAAACGCGTCTTGCGGACGAATTCTTAGGTGAAATTCCTGAAAAATTAAGA
CGTAGAGTTAGGTTTTTACG

Downstream 100 bases:

>100_bases
AAGGGAGACGATTGATATGGCAAAAGGTAATTTATTTAAAGCGATTTTAGGTATAGGTGGCGCTGTAGCAGCTGTACTTG
TTACACGTAAAGATAGTCGT

Product: pseudouridine synthase family 1 protein

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPENDKITVRGELIEYIENVYIMLNKPKGYISATEDHHS
KTVIDLIPEYQHLNIFPVGRLDKDTEGLLLITNDGDFNHELMSPNKHVSKKYEVISANPITEDDIQAFKEGVTLTDGKVK
PAILTYIDNQTSHVTIYEGKYHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYRLLTENDFDKLNYK

Sequences:

>Translated_231_residues
MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPENDKITVRGELIEYIENVYIMLNKPKGYISATEDHHS
KTVIDLIPEYQHLNIFPVGRLDKDTEGLLLITNDGDFNHELMSPNKHVSKKYEVISANPITEDDIQAFKEGVTLTDGKVK
PAILTYIDNQTSHVTIYEGKYHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYRLLTENDFDKLNYK
>Mature_231_residues
MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPENDKITVRGELIEYIENVYIMLNKPKGYISATEDHHS
KTVIDLIPEYQHLNIFPVGRLDKDTEGLLLITNDGDFNHELMSPNKHVSKKYEVISANPITEDDIQAFKEGVTLTDGKVK
PAILTYIDNQTSHVTIYEGKYHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYRLLTENDFDKLNYK

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=227, Percent_Identity=37.0044052863436, Blast_Score=154, Evalue=3e-39,
Organism=Escherichia coli, GI1787524, Length=243, Percent_Identity=30.8641975308642, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI1790453, Length=228, Percent_Identity=30.7017543859649, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI87081838, Length=180, Percent_Identity=32.7777777777778, Blast_Score=92, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26616; Mature: 26616

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPENDKITVRGELIEYIEN
CCHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCEEEEHHHHHHHHCC
VYIMLNKPKGYISATEDHHSKTVIDLIPEYQHLNIFPVGRLDKDTEGLLLITNDGDFNHE
EEEEEECCCCCEECCCCCCCCCCEECCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCH
LMSPNKHVSKKYEVISANPITEDDIQAFKEGVTLTDGKVKPAILTYIDNQTSHVTIYEGK
HCCCCHHHHHHEEEEECCCCCHHHHHHHHCCCEEECCCCCCEEEEEECCCCCEEEEEECC
YHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYRLLTENDFDKLNYK
HHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPENDKITVRGELIEYIEN
CCHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCEEEEHHHHHHHHCC
VYIMLNKPKGYISATEDHHSKTVIDLIPEYQHLNIFPVGRLDKDTEGLLLITNDGDFNHE
EEEEEECCCCCEECCCCCCCCCCEECCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCH
LMSPNKHVSKKYEVISANPITEDDIQAFKEGVTLTDGKVKPAILTYIDNQTSHVTIYEGK
HCCCCHHHHHHEEEEECCCCCHHHHHHHHCCCEEECCCCCCEEEEEECCCCCEEEEEECC
YHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYRLLTENDFDKLNYK
HHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]