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The map label for this gene is mrcA [H]

Identifier: 57652028

GI number: 57652028

Start: 1820270

End: 1821175

Strand: Direct

Name: mrcA [H]

Synonym: SACOL1779

Alternate gene names: 57652028

Gene position: 1820270-1821175 (Clockwise)

Preceding gene: 57652026

Following gene: 57652032

Centisome position: 64.79

GC content: 30.13

Gene sequence:

>906_bases
ATGACGAATCAAGACAACAATCATCAATTGAATCATCGTATATATCATTTTGAAAAGATATATAAAGCTATCAAACATGT
CATTGTTTTTATATTTATGATTTTCATTGCCATCGTTGCTATCGCTGTGATTGCGATGTCTTTATATTTTCATCATTTAA
CTAAAACGTCCGACTCATTATCAGATGATGCTTTAATAAAAAAAGTTCGACAAATACCTGGCGATGAATTATTAGATCAT
AATAACAAAAATTTATTATATGAGTATAACCATTCTCAAAACTCACTCATTATAGGCCCTAAAACATCAAGTCCAAATGT
CATTAAAGCATTAACGTCATCTGAAGACACTTTATTTTATAAACATGATGGCATCTTACCAAAGGCGATTTTAAGAGCAA
TGATACAAGATATTTTTAATACTGATCAAAGTTCAGGTGGTAGCACAATTACACAACAACTTGTTAAAAATCAAGTTCTT
ACCAACGAAAAAACATATAGTAGAAAAGCAAATGAACTTCGCCTAGCAATTAGATTAGAACACCTACTCTCAAAAGATGA
AATTATATATACATATTTAAATATAGTTCCCTTCGGTAAAGATTATAATGGCGCTAATATTTCCGGAATTGCATCCGCTT
CATATAGTCTATTTGGTATTCCACCAAAAGATTTATCAATTGCACAATCTGCATACCTTATCGGTTTGTTGCAAAGCCCA
TATGGCTATACACCCTACGAAAAAGATGGAACGTTAAAATCGGATAAAGATTTGAAATATAGTATTCAAAGACAACATTA
TGTATTAAAGCGTATGTTAATCGAAGATCAAATCACTGAAAAAGAATACAACGACGCATTAAAATATGATATTAAATCAC
ATTTGTTAAATCGAAAAAAGCGTTAA

Upstream 100 bases:

>100_bases
TATCCTATTCTTTATGCATTTTCCCTAATTATAATTAACGTTAAAATAAAAGTCAAATTGCTTAAATATGGTATACTATA
CGTAATTTAGGAGGTTAAAG

Downstream 100 bases:

>100_bases
TTGATGCTCACTTTTTAAAGTAACCACAACAATGAATCCAAATATTAAAAACAGCAGTAAGATTATTTTCAATTAGAAAA
TTTCTCACTGCTGTTCTTTT

Product: transglycosylase domain-containing protein

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MTNQDNNHQLNHRIYHFEKIYKAIKHVIVFIFMIFIAIVAIAVIAMSLYFHHLTKTSDSLSDDALIKKVRQIPGDELLDH
NNKNLLYEYNHSQNSLIIGPKTSSPNVIKALTSSEDTLFYKHDGILPKAILRAMIQDIFNTDQSSGGSTITQQLVKNQVL
TNEKTYSRKANELRLAIRLEHLLSKDEIIYTYLNIVPFGKDYNGANISGIASASYSLFGIPPKDLSIAQSAYLIGLLQSP
YGYTPYEKDGTLKSDKDLKYSIQRQHYVLKRMLIEDQITEKEYNDALKYDIKSHLLNRKKR

Sequences:

>Translated_301_residues
MTNQDNNHQLNHRIYHFEKIYKAIKHVIVFIFMIFIAIVAIAVIAMSLYFHHLTKTSDSLSDDALIKKVRQIPGDELLDH
NNKNLLYEYNHSQNSLIIGPKTSSPNVIKALTSSEDTLFYKHDGILPKAILRAMIQDIFNTDQSSGGSTITQQLVKNQVL
TNEKTYSRKANELRLAIRLEHLLSKDEIIYTYLNIVPFGKDYNGANISGIASASYSLFGIPPKDLSIAQSAYLIGLLQSP
YGYTPYEKDGTLKSDKDLKYSIQRQHYVLKRMLIEDQITEKEYNDALKYDIKSHLLNRKKR
>Mature_300_residues
TNQDNNHQLNHRIYHFEKIYKAIKHVIVFIFMIFIAIVAIAVIAMSLYFHHLTKTSDSLSDDALIKKVRQIPGDELLDHN
NKNLLYEYNHSQNSLIIGPKTSSPNVIKALTSSEDTLFYKHDGILPKAILRAMIQDIFNTDQSSGGSTITQQLVKNQVLT
NEKTYSRKANELRLAIRLEHLLSKDEIIYTYLNIVPFGKDYNGANISGIASASYSLFGIPPKDLSIAQSAYLIGLLQSPY
GYTPYEKDGTLKSDKDLKYSIQRQHYVLKRMLIEDQITEKEYNDALKYDIKSHLLNRKKR

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=257, Percent_Identity=32.6848249027237, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1786343, Length=150, Percent_Identity=38.6666666666667, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788867, Length=188, Percent_Identity=32.4468085106383, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789601, Length=169, Percent_Identity=28.9940828402367, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.- [C]

Molecular weight: Translated: 34499; Mature: 34368

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNQDNNHQLNHRIYHFEKIYKAIKHVIVFIFMIFIAIVAIAVIAMSLYFHHLTKTSDSL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SDDALIKKVRQIPGDELLDHNNKNLLYEYNHSQNSLIIGPKTSSPNVIKALTSSEDTLFY
CHHHHHHHHHHCCCHHHHCCCCCCEEEEECCCCCEEEEECCCCCCHHHHHCCCCCCEEEE
KHDGILPKAILRAMIQDIFNTDQSSGGSTITQQLVKNQVLTNEKTYSRKANELRLAIRLE
ECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
HLLSKDEIIYTYLNIVPFGKDYNGANISGIASASYSLFGIPPKDLSIAQSAYLIGLLQSP
HHHCCCCEEEEEEEEEECCCCCCCCCCCCCHHCCCEEECCCCCCHHHHHHHHHHHHHCCC
YGYTPYEKDGTLKSDKDLKYSIQRQHYVLKRMLIEDQITEKEYNDALKYDIKSHLLNRKK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
R
C
>Mature Secondary Structure 
TNQDNNHQLNHRIYHFEKIYKAIKHVIVFIFMIFIAIVAIAVIAMSLYFHHLTKTSDSL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SDDALIKKVRQIPGDELLDHNNKNLLYEYNHSQNSLIIGPKTSSPNVIKALTSSEDTLFY
CHHHHHHHHHHCCCHHHHCCCCCCEEEEECCCCCEEEEECCCCCCHHHHHCCCCCCEEEE
KHDGILPKAILRAMIQDIFNTDQSSGGSTITQQLVKNQVLTNEKTYSRKANELRLAIRLE
ECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
HLLSKDEIIYTYLNIVPFGKDYNGANISGIASASYSLFGIPPKDLSIAQSAYLIGLLQSP
HHHCCCCEEEEEEEEEECCCCCCCCCCCCCHHCCCEEECCCCCCHHHHHHHHHHHHHCCC
YGYTPYEKDGTLKSDKDLKYSIQRQHYVLKRMLIEDQITEKEYNDALKYDIKSHLLNRKK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3882429; 9278503; 3920658; 7006606; 9841666 [H]