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The map label for this gene is glyS

Identifier: 57651958

GI number: 57651958

Start: 1653436

End: 1654827

Strand: Direct

Name: glyS

Synonym: SACOL1622

Alternate gene names: 57651958

Gene position: 1653436-1654827 (Clockwise)

Preceding gene: 57651928

Following gene: 57651978

Centisome position: 58.85

GC content: 34.27

Gene sequence:

>1392_bases
ATGGCAAAAGATATGGATACAATTGTTTCATTAGCAAAACACAGAGGTTTTGTGTTCCCTGGTAGTGATATTTACGGTGG
TTTATCAAACACATGGGATTATGGTCCTTTAGGTGTTGAATTAAAGAATAATGTTAAAAAAGCTTGGTGGCAAAAATTCA
TTACACAATCACCGTTTAACGTTGGTATCGATGCTGCAATCTTAATGAATCCAAAAGTATGGGAAGCTTCAGGACACTTA
AACAACTTCAACGACCCAATGATTGATAATAAAGATAGTAAAATTCGATATCGCGCTGATAAATTAATTGAAGATTATAT
GCAAGATGTTAAAGGTGATGAAAACTTCATTGCCGATGGTTTAAGTTTTGAACAAATGAAAAAAATTATTGACGATGAAG
GTATTGTTTGTCCTGTAAGTAAAACTGCTAACTGGACTGAAATTCGCCAATTCAATTTAATGTTTAAAACATTCCAAGGT
GTAACTGAAGATTCTACAAATGAAATTTTCTTACGTCCTGAAACAGCACAAGGTATTTTTGTAAACTATAAAAACGTGCA
ACGTTCAATGCGTAAAAAATTACCATTTGGTATCGGTCAAATTGGTAAATCATTCCGTAATGAAATCACTCCAGGTAACT
TCATTTTCAGAACAAGAGAATTTGAACAAATGGAACTTGAATTCTTCTGTAAACCTGGAGAAGAAATCGAATGGCAAAAT
TATTGGAAAACTTTTGCAAGTGACTGGTTAACAAGCTTAAATATGAGCAGTGAAAATATGCGTTTACGTGATCATGATGA
AGATGAATTATCTCATTACTCAAATGCAACAACTGATATTGAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTA
TCGCAAGTCGTACAGACTTCGACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACG
AACGAAAAATATATTCCATATTGTATCGAGCCATCACTTGGTGCAGATCGTGTAACATTAGCTTTCTTATGTGATGCATA
TGATGAAGAAGGCGTTGAAGGTAGTAAAGATGCACGTACAGTTTTACACTTCCATCCTGCATTAGCACCATATAAAGCAG
CGATTTTACCTTTAAGTAAGAAATTATCTGGCGAAGCGATTAAGATTTTTGAGCAATTAAGTTCTAAATTCTCAATCGAT
TTCGATGAATCACAATCTATCGGTAAAAGATACCGTCGTCAAGATGAAATCGGTACACCTTATTGTGTAACATTCGACTT
TGATTCATTAGAAGATAATCAAGTTACAGTACGTGACAGAGATTCAATGGAACAAGTTCGTATGCCAATCTCAGAGTTAG
AAGCTTTCTTAACTGAAAAAACAAAATTCTAA

Upstream 100 bases:

>100_bases
TATATACAATTTAAAATATTAACTCGTCCCATGGAAGTAAAGCATATTTACTATGCTGTTATTTTCCATGGGGCGTTTTC
ATGTATGAGGAGAGGTAATT

Downstream 100 bases:

>100_bases
TTAAAAAGACTTTCCACCATATGTGGTGGCTATATAATTTATAAAAAGAGATACTGGTTAGTAAAATTGCCAGTATCTCT
TTTCTATTCAAAAAGGTATT

Product: glycyl-tRNA synthetase

Products: NA

Alternate protein names: Glycine--tRNA ligase; GlyRS

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNNVKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHL
NNFNDPMIDNKDSKIRYRADKLIEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQG
VTEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRTREFEQMELEFFCKPGEEIEWQN
YWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNATTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPET
NEKYIPYCIEPSLGADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQLSSKFSID
FDESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPISELEAFLTEKTKF

Sequences:

>Translated_463_residues
MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNNVKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHL
NNFNDPMIDNKDSKIRYRADKLIEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQG
VTEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRTREFEQMELEFFCKPGEEIEWQN
YWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNATTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPET
NEKYIPYCIEPSLGADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQLSSKFSID
FDESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPISELEAFLTEKTKF
>Mature_462_residues
AKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNNVKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHLN
NFNDPMIDNKDSKIRYRADKLIEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQGV
TEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRTREFEQMELEFFCKPGEEIEWQNY
WKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNATTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPETN
EKYIPYCIEPSLGADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQLSSKFSIDF
DESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPISELEAFLTEKTKF

Specific function: Catalyzes the attachment of glycine to tRNA(Gly)

COG id: COG0423

COG function: function code J; Glycyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI116805340, Length=390, Percent_Identity=31.025641025641, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI70887790, Length=445, Percent_Identity=22.9213483146067, Blast_Score=89, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17554996, Length=396, Percent_Identity=28.2828282828283, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI32564541, Length=396, Percent_Identity=28.2828282828283, Blast_Score=157, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6325338, Length=365, Percent_Identity=30.4109589041096, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6319597, Length=393, Percent_Identity=27.4809160305344, Blast_Score=142, Evalue=1e-34,
Organism=Drosophila melanogaster, GI21357965, Length=355, Percent_Identity=31.2676056338028, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24664462, Length=355, Percent_Identity=31.2676056338028, Blast_Score=172, Evalue=5e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYG_STAA1 (A7X2W2)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442142.1
- ProteinModelPortal:   A7X2W2
- SMR:   A7X2W2
- STRING:   A7X2W2
- EnsemblBacteria:   EBSTAT00000003944
- GeneID:   5560133
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1552
- eggNOG:   COG0423
- GeneTree:   EBGT00050000024915
- HOGENOM:   HBG319978
- OMA:   GSEWGEL
- ProtClustDB:   PRK04173
- BioCyc:   SAUR418127:SAHV_1552-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00253_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR022961
- InterPro:   IPR002315
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR10745
- PRINTS:   PR01043
- TIGRFAMs:   TIGR00389

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.14

Molecular weight: Translated: 53621; Mature: 53489

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: BINDING 98-98 BINDING 174-174

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNNVKKAWWQKFITQSPFN
CCCHHHHHHHHHHHCCEECCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
VGIDAAILMNPKVWEASGHLNNFNDPMIDNKDSKIRYRADKLIEDYMQDVKGDENFIADG
CCCCEEEEECCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHCCC
LSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQGVTEDSTNEIFLRPETAQGIF
CCHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEE
VNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRTREFEQMELEFFCKPGEEIEWQN
EEHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECCCHHHCCEEEEECCCCCCCHHH
YWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNATTDIEYKFPFGWGELWGIASRTDF
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCHHHHHCCCCCCCH
DLRKHAEHSGEDFRYHDPETNEKYIPYCIEPSLGADRVTLAFLCDAYDEEGVEGSKDART
HHHHHHCCCCCCCEECCCCCCCCCCCEEECCCCCCCCEEEEHHHHHHCCCCCCCCCCHHE
VLHFHPALAPYKAAILPLSKKLSGEAIKIFEQLSSKFSIDFDESQSIGKRYRRQDEIGTP
EEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHCCCCC
YCVTFDFDSLEDNQVTVRDRDSMEQVRMPISELEAFLTEKTKF
EEEEEECCCCCCCEEEECCCHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNNVKKAWWQKFITQSPFN
CCHHHHHHHHHHHCCEECCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
VGIDAAILMNPKVWEASGHLNNFNDPMIDNKDSKIRYRADKLIEDYMQDVKGDENFIADG
CCCCEEEEECCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHCCC
LSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQGVTEDSTNEIFLRPETAQGIF
CCHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEE
VNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRTREFEQMELEFFCKPGEEIEWQN
EEHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECCCHHHCCEEEEECCCCCCCHHH
YWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNATTDIEYKFPFGWGELWGIASRTDF
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCHHHHHCCCCCCCH
DLRKHAEHSGEDFRYHDPETNEKYIPYCIEPSLGADRVTLAFLCDAYDEEGVEGSKDART
HHHHHHCCCCCCCEECCCCCCCCCCCEEECCCCCCCCEEEEHHHHHHCCCCCCCCCCHHE
VLHFHPALAPYKAAILPLSKKLSGEAIKIFEQLSSKFSIDFDESQSIGKRYRRQDEIGTP
EEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHCCCCC
YCVTFDFDSLEDNQVTVRDRDSMEQVRMPISELEAFLTEKTKF
EEEEEECCCCCCCEEEECCCHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA