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The map label for this gene is dnaG [H]

Identifier: 57651955

GI number: 57651955

Start: 1649789

End: 1651588

Strand: Reverse

Name: dnaG [H]

Synonym: SACOL1619

Alternate gene names: 57651955

Gene position: 1651588-1649789 (Counterclockwise)

Preceding gene: 57651956

Following gene: 57651954

Centisome position: 58.79

GC content: 31.61

Gene sequence:

>1800_bases
TTGCGAATAGATCAATCGATCATTAATGAAATAAAAGATAAAACCGACATTTTAGACTTGGTAAGTGAATATGTAAAATT
AGAAAAGAGAGGACGCAATTATATAGGTTTGTGTCCTTTTCATGATGAAAAGACACCTTCATTTACAGTTTCTGAAGATA
AACAAATTTGTCATTGTTTTGGTTGTAAAAAAGGTGGCAATGTTTTTCAATTTACTCAAGAAATTAAAGACATATCATTT
GTTGAAGCGGTTAAAGAATTAGGTGATAGAGTTAATGTTGCTGTAGATATTGAGGCAACACAATCTAACTCAAATGTTCA
AATTGCTTCTGATGATTTACAAATGATTGAAATGCATGAGTTAATACAAGAATTTTATTATTACGCTTTAACAAAGACAG
TCGAAGGCGAACAAGCATTAACGTACTTACAAGAACGTGGTTTTACAGATGCGCTTATTAAAGAGCGAGGCATTGGCTTT
GCACCCGATAGCTCACATTTTTGTCATGATTTTCTTCAAAAAAAGGGTTACGATATTGAATTAGCATATGAAGCCGGATT
ATTATCACGTAACGAAGAAAATTTCAGTTATTACGATAGATTTCGAAATCGTATTATGTTTCCTTTGAAAAATGCGCAAG
GAAGAATTGTTGGATATTCAGGTCGAACATATACCGGTCAAGAACCAAAATACTTAAATAGTCCTGAAACACCTATCTTT
CAAAAAAGAAAGTTGTTATACAACTTAGATAAAGCGCGTAAATCAATTAGAAAATTAGATGAAATCGTATTACTAGAAGG
TTTTATGGATGTTATAAAATCTGATACTGCTGGCTTGAAAAACGTTGTTGCAACAATGGGTACACAGTTGTCAGATGAAC
ATATTACTTTTATACGAAAGTTAACATCAAATATAACATTAATGTTTGATGGGGATTTTGCGGGTAGTGAAGCAACACTT
AAAACAGGTCAAAATTTGTTACAGCAAGGGCTAAATGTATTTGTTATACAATTGCCATCAGGCATGGATCCGGATGAATA
CATTGGTAAGTATGGCAACGATGCATTTACTGCTTTTGTAAAAAATGACAAAAAGTCATTTGCACATTATAAAGTGAGTA
TATTAAAAGATGAAATTGCACATAATGACCTTTCATATGAACGTTATTTGAAAGAACTAAGTCATGATATTTCGCTTATG
AAATCATCGATTTTGCAACAAAAGGCTTTAAATGATGTTGCACCATTTTTCAATGTTAGTCCTGAGCAATTAGCTAACGA
AATACAATTCAATCAAGCACCAGCCAATTATTATCCAGAAGATGAGTATGGCGGTTACATTGAACCTGAGCCAATTGGTA
TGGCACAATTTGACAATTTGAGCCGTCAAGAAAAAGCGGAGCGAGCATTTTTAAAACATTTAATGAGAGATAAAGATACA
TTTTTAAATTATTATGAAAGTGTTGATAAGGATAACTTCACAAATCAGCATTTTAAATATGTATTCGAAGTCTTACATGA
TTTTTATGCGGAAAATGATCAATATAATATCAGTGATGCTGTGCAGTATGTTAATTCAAATGAGTTGAGAGAAACACTAA
TTAGCTTAGAACAATATAATTTGAATGACGAACCATATGAAAATGAAATTGATGATTATGTCAATGTTATTAATGAAAAA
GGACAAGAAACAATTGAGTCATTGAATCATAAATTAAGGGAAGCTACAAGGATTGGCGATGTAGAATTACAAAAATACTA
TTTACAGCAAATTGTTGCTAAGAATAAAGAACGCATGTAG

Upstream 100 bases:

>100_bases
CGATATAATCCATTATATTGAACAAAATAAATCGAAATGATTTCATTTTTGTCGAAAATTAGGTATAATAGTATAACTAA
TGCTTAATAGGTGATTTAAT

Downstream 100 bases:

>100_bases
CATGTGATTTTAAAGAATAATACGAATAATGATTATGTCAAAATGTATAAGGGTAAATGATAGTTACCGCATTTAAACAA
CACTATTGAAAAATAAATAT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEKTPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISF
VEAVKELGDRVNVAVDIEATQSNSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVGYSGRTYTGQEPKYLNSPETPIF
QKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDTAGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATL
KTGQNLLQQGLNVFVIQLPSGMDPDEYIGKYGNDAFTAFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKELSHDISLM
KSSILQQKALNDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYIEPEPIGMAQFDNLSRQEKAERAFLKHLMRDKDT
FLNYYESVDKDNFTNQHFKYVFEVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYVNVINEK
GQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM

Sequences:

>Translated_599_residues
MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEKTPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISF
VEAVKELGDRVNVAVDIEATQSNSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVGYSGRTYTGQEPKYLNSPETPIF
QKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDTAGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATL
KTGQNLLQQGLNVFVIQLPSGMDPDEYIGKYGNDAFTAFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKELSHDISLM
KSSILQQKALNDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYIEPEPIGMAQFDNLSRQEKAERAFLKHLMRDKDT
FLNYYESVDKDNFTNQHFKYVFEVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYVNVINEK
GQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM
>Mature_599_residues
MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEKTPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISF
VEAVKELGDRVNVAVDIEATQSNSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVGYSGRTYTGQEPKYLNSPETPIF
QKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDTAGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATL
KTGQNLLQQGLNVFVIQLPSGMDPDEYIGKYGNDAFTAFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKELSHDISLM
KSSILQQKALNDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYIEPEPIGMAQFDNLSRQEKAERAFLKHLMRDKDT
FLNYYESVDKDNFTNQHFKYVFEVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYVNVINEK
GQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=364, Percent_Identity=37.9120879120879, Blast_Score=245, Evalue=7e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007693
- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF00772 DnaB; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 69295; Mature: 69295

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEKTPSFTVSEDKQICHCF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEECCCCHHHHHHH
GCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQSNSNVQIASDDLQMIEMHE
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEEEECCHHHHHHHHH
LIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGFAPDSSHFCHDFLQKKGYDIE
HHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEE
LAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVGYSGRTYTGQEPKYLNSPETPIF
EEECCCCCCCCCCCHHHHHHHHHCEECEEECCCCCEEECCCCCCCCCCCCCCCCCCCCHH
QKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDTAGLKNVVATMGTQLSDEHITFIRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHH
LTSNITLMFDGDFAGSEATLKTGQNLLQQGLNVFVIQLPSGMDPDEYIGKYGNDAFTAFV
HHCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCCCCEEEEH
KNDKKSFAHYKVSILKDEIAHNDLSYERYLKELSHDISLMKSSILQQKALNDVAPFFNVS
HCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PEQLANEIQFNQAPANYYPEDEYGGYIEPEPIGMAQFDNLSRQEKAERAFLKHLMRDKDT
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHH
FLNYYESVDKDNFTNQHFKYVFEVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYN
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHHHHHHHHHHHCC
LNDEPYENEIDDYVNVINEKGQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM
CCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEKTPSFTVSEDKQICHCF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEECCCCHHHHHHH
GCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQSNSNVQIASDDLQMIEMHE
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEEEECCHHHHHHHHH
LIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGFAPDSSHFCHDFLQKKGYDIE
HHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEE
LAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVGYSGRTYTGQEPKYLNSPETPIF
EEECCCCCCCCCCCHHHHHHHHHCEECEEECCCCCEEECCCCCCCCCCCCCCCCCCCCHH
QKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDTAGLKNVVATMGTQLSDEHITFIRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHH
LTSNITLMFDGDFAGSEATLKTGQNLLQQGLNVFVIQLPSGMDPDEYIGKYGNDAFTAFV
HHCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCCCCEEEEH
KNDKKSFAHYKVSILKDEIAHNDLSYERYLKELSHDISLMKSSILQQKALNDVAPFFNVS
HCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PEQLANEIQFNQAPANYYPEDEYGGYIEPEPIGMAQFDNLSRQEKAERAFLKHLMRDKDT
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHH
FLNYYESVDKDNFTNQHFKYVFEVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYN
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHHHHHHHHHHHCC
LNDEPYENEIDDYVNVINEKGQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM
CCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]