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The map label for this gene is nfo [H]

Identifier: 57651950

GI number: 57651950

Start: 1644188

End: 1645078

Strand: Reverse

Name: nfo [H]

Synonym: SACOL1614

Alternate gene names: 57651950

Gene position: 1645078-1644188 (Counterclockwise)

Preceding gene: 57651951

Following gene: 57651949

Centisome position: 58.56

GC content: 33.22

Gene sequence:

>891_bases
ATGTTATTAGGATCACATGTTTCAATGAGTGGTAAAAAGATGTTAGAAGGTTCTGCTATAGAAGCGCATGAATATGGTGA
AACAACATTTATGATTTATACTGGTGCACCTCAAAACACGCGCCGTAAAAGTATTGAAGATTTAAATATAACTAAAGGTC
ATGAAGTGATGGAAAAATATGGTTTATCTAATATTGTTGTTCATGCACCATACATCATTAATATTGCAAATACAACCAAA
CCAGAAACGTTTAATCTTGGTGTAGATTTCTTGCAACAAGAAATTGAAAGAACGCAAGCTATAGGGGCTAAAGATATTGT
ATTACATCCAGGTGCGCATGTTGGAGCTGGTGTTGATGCCGGAATTAATAAGATTATTGAAGGATTAAATGAAGTTTTAA
CGAATGACAATAATGTACGTATTGCGCTTGAAACGATGGCAGGTAAAGGTACAGAGATTGGTCGCTCATTTGAAGAGTTA
GCACGTATTATTGATGGAGTTCATAATAACGAACGTTTATCAGTATGTTTTGATACATGTCATACGCATGATGCTGGATA
TAATGTTAAAGAGGACTTTGATGGCGTATTAAATGAATTTGATAAAATCATTGGAGTCGACAGAATCAAAGTTGTTCATG
TCAATGATTCTAAAAATGACCGTGGCGCTCAAAAAGACCGACATGAAAATATTGGCTTCGGTTACATTGGTTTTGATGCA
TTAAATTACATTGTGCATCATGATAGTTTTAAAGATATTCCGAAAATCTTAGAAACACCTTATGTTGGTGAAGATAAGAA
AAATAAAAAACCGCCATATAAACTTGAAATTGAAATGTTAAAACAACAGCAATTTGACCCAGAATTAAAAAATAAGGTTA
TGCAACAATAA

Upstream 100 bases:

>100_bases
ACAAGAAGTTGAAAAAATGAAACGTCAAGAGCGTAAGCAATTTAGTAAGCAGCAAAATAGACAAAAACGTAAGCAAAACA
AAAAAGGTTAGGTGTGTTTT

Downstream 100 bases:

>100_bases
AATGATAATGCTCTCTTCAAGAAATGCGATAAATAAAACTTGAAGGGGGCATATCTCTTTATTTTGTCTAATTTTGAGTC
GTAAACATTACTGTTTACAT

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MLLGSHVSMSGKKMLEGSAIEAHEYGETTFMIYTGAPQNTRRKSIEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTK
PETFNLGVDFLQQEIERTQAIGAKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSKNDRGAQKDRHENIGFGYIGFDA
LNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLEIEMLKQQQFDPELKNKVMQQ

Sequences:

>Translated_296_residues
MLLGSHVSMSGKKMLEGSAIEAHEYGETTFMIYTGAPQNTRRKSIEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTK
PETFNLGVDFLQQEIERTQAIGAKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSKNDRGAQKDRHENIGFGYIGFDA
LNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLEIEMLKQQQFDPELKNKVMQQ
>Mature_296_residues
MLLGSHVSMSGKKMLEGSAIEAHEYGETTFMIYTGAPQNTRRKSIEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTK
PETFNLGVDFLQQEIERTQAIGAKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSKNDRGAQKDRHENIGFGYIGFDA
LNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLEIEMLKQQQFDPELKNKVMQQ

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=289, Percent_Identity=32.1799307958478, Blast_Score=148, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17531193, Length=264, Percent_Identity=32.5757575757576, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6322735, Length=264, Percent_Identity=31.4393939393939, Blast_Score=145, Evalue=7e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 33159; Mature: 33159

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00729 AP_NUCLEASE_F2_1 ; PS00730 AP_NUCLEASE_F2_2 ; PS00731 AP_NUCLEASE_F2_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLGSHVSMSGKKMLEGSAIEAHEYGETTFMIYTGAPQNTRRKSIEDLNITKGHEVMEKY
CCCCCCCCCCCHHHHCCCCCCHHHCCCEEEEEEECCCCCHHHHCCCCCCCCCHHHHHHHH
GLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIGAKDIVLHPGAHVGAGVDA
CCCCEEEECCEEEEECCCCCCCEEECCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHH
GINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEELARIIDGVHNNERLSVCFDTC
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEHH
HTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSKNDRGAQKDRHENIGFGYIGFDA
HCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHCCCCCEEEHHHH
LNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLEIEMLKQQQFDPELKNKVMQQ
HHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MLLGSHVSMSGKKMLEGSAIEAHEYGETTFMIYTGAPQNTRRKSIEDLNITKGHEVMEKY
CCCCCCCCCCCHHHHCCCCCCHHHCCCEEEEEEECCCCCHHHHCCCCCCCCCHHHHHHHH
GLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIGAKDIVLHPGAHVGAGVDA
CCCCEEEECCEEEEECCCCCCCEEECCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHH
GINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEELARIIDGVHNNERLSVCFDTC
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEHH
HTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSKNDRGAQKDRHENIGFGYIGFDA
HCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHCCCCCEEEHHHH
LNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLEIEMLKQQQFDPELKNKVMQQ
HHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA